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Tissue References Search Tool

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Stem
Human
Human
Gentile, P. , Orlandi, A. , Scioli, M. , Di Pasquali, C. , Bocchini, I. and Cervelli, V.
Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery. ,
Stem Cells Transl Med
 1 , 230-6 , 2012
Cells
Adipose derived stromal vascular
Enzymes
Medium
PBS
Stem
Human
Human
Salehinejad, P. , Alitheen, N. , Ali, A. , Omar, A. , Mohit, M. , Janzamin, E. , Samani, F. , Torshizi, Z. and Nematollahi-Mahani, S.
Comparison of Different Methods for the Isolation of Mesenchymal Stem Cells from Human Umbilical Cord Wharton's Jelly. ,
In Vitro Cell Dev Biol Anim
 48 , 75 , 2012
Cells
Wharton jelly mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM
Stem
Human
Human
Sakai, K. , Yamamoto, A. , Matsubara, K. , Nakamura, S. , Naruse, M. , Yamagata, M. , Sakamoto, K. , Tauchi, R. , Wakao, N. , Imagama, S. , Hibi, H. , Kadomatsu, K. , Ishiguro, N. and Ueda, M
Human Dental Pulp-Derived Stem Cells Promote Locomotor Recovery After Complete Transection of the Rat Spinal Cord by Multiple Neuro-Regenerative Mechanisms. ,
J Clin Invest
 122 , 80 , 2012
Cells
Dental pulp derived stem
Enzymes
Medium
DMEM
Stem
Human
Human
Yang, X. , He, X. , He, J. , Zhang, L. , Su, X. , Dong, Z. , Xu, Y. , Li, Y. and Li, YL.
High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells. ,
J Biomed Sci
 18 , 59 , 2011
Cells
Adipose derived mesenchymal
Enzymes
Medium
L-DMEM
File
10930.pdf (7.71 MB)
Stem
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
File
11598.pdf (768.38 KB)
Stem
Human
Human
Klein, D. , Weisshardt, P. , Kleff, V. , Jastrow, H. , Jakob, H. and Ergun, S.
Vascular Wall-Resident CD44+ Multipotent Stem Cells Vive Rise to Pericytes and Smooth Muscle Cells and Contribute to New Vessel Maturation. ,
PLoS ONE
 6 , e20540 , 2011
Cells
Vascular wall-resident multipotent stem cells
Enzymes
Elastase : 5 u/ml
Medium
see reference
File
10879.pdf (1009.69 KB)
Stem
Human
Human
Yu, G. Floyd, ZE. , Wu, X. , Halvorsen, Y. and Gimble, J.
Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates. ,
Methods Mol Biol
 702 , 17-27 , 2011
Cells
Adipose derived stem
Enzymes
Medium
DMEM/Ham's F-12
Stem
Human
Human, male 40-60 years
Blasi, A. , Martino, C. , Balducci, L. , Saldarelli, M. , Soleti, A. , Navone, S. , Canzi, L. , Cristini, S. , Invernici, G. , Parati, E. and Alessandri, G.
Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential ,
Vasc Cell
 3 , 5 , 2011
Cells
Adipose derived stem cells
Enzymes
Collagenase : 0.25%
Medium
PBS
Stem
Human
Human
Farias, V. , Linares-Fernandez, J. , Penalver, J. , Paya Colmenero, J. , Ferron, G. , Duran, E. , Fernandez, R. , Olivares, E. , O'Valle, F. , Puertas, A. , Oliver, F. and Ruiz de Almodovar, J.
Human Umbilical Cord Stromal Stem Cell Express CD10 and Exert Contractile Properties. ,
Placenta
 32 , 86 , 2011
Cells
Umbilical cord stromal stem
Enzymes
Medium
DMEM
Stem
Human
Human
Yu, G. , Wu, X. , Dietrich, M. , Polk, P. , Scott, LK, Ptitsyn, A. and Gimble, J.
Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes. ,
Cytotherapy
 12 , 538 , 2010
Cells
Adipose stromal
Enzymes
Medium
DMEM/Hams F-12
File
11463.pdf (7.32 MB)
Stem
Human
Human
Roig, A. , Eskiocak, U. , Hight, S. , Kim, S. , Delgado, O. , Souza, R. , Spechler, S. , Wright, W. and Shay, J.
Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro. ,
Gastroenterol
 138 , 1012 , 2010
Cells
Colonic epithelial
Enzymes
Collagenase : 150 u/ml
Neutral Protease : 0.04 mg/ml
Medium
Basal X media
Stem
Human
Human
Tan, H. , DeFail, A. , Rubin, J. , Chu, C. and Marra, K.
Novel Multiarm PEG-Based Hydrogels for Tissue Engineering. ,
J Biomed Mater Res
 92 , 979 , 2010
Cells
Adipose tissue-derived stem
Enzymes
Medium
DMEM/F12
File
10925.pdf (704.73 KB)
Stem
Human
Human
Zeddou, M. , Briquet, A. , Relic, B. , Josse, C. , Malaise, M. , Gothot, A. , Lechanteur, C. and Beguin, Y.
The Umbilical Cord Matrix is a Better Source of Mesenchymal Stem Cells (MSC) Than the Umbilical Cord Blood. ,
Cell Biol Int
 34 , 693-701 , 2010
Cells
Umbilical cord mesenchymal
Enzymes
Hyaluronidase : 0.05%
Collagenase : 0.08%
Medium
DMEM
File
10929.pdf (709.43 KB)
Stem
Human
Human
Hareendran, S. , Sathishkumar, S. , Abbas, S. , Mackay, A. and Rajan, P.
A Novel Composition for the Culture of Human Adipose Stem Cells which Includes Complement C3. ,
Cytotechnology
 62 , 389-402 , 2010
Cells
Adipose stem
Enzymes
Medium
DMEM
File
11001.pdf (633.5 KB)
Stem
Human
Human
Huang, G. , Yamaza, T. , Shea, L. , Djouad, F. , Kuhn, N. , Tuan, R. and Shi, S.
Stem/Progenitor Cell-Mediated De Novo Regeneration of Dental Pulp with Newly Deposited Continuous Layer of Dentin in an In Vivo Model. ,
Tissue Eng Part A
 16 , 605 , 2010
Cells
Dental pulp and apical papilla stem cells
Enzymes
Medium
MEM
Stem
Human
Human
Du, Y. , Roh, D. , Funderburgh, M. , Mann, M. , Marra, K. , Rubin, J. , Li, X. and Funderburgh, J.
Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. ,
Mol Vis
 16 , 2680 , 2010
Cells
Corneal stromal stem
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase : 0.1%
Medium
DMEM
File
10602.pdf (1.08 MB)
Stem
Human
Human
Hjelmeland, A. , Wu, Q. , Wickman, S. , Eyler, C. , Heddleston, J. , Shi, Q. , Lathia, J. , Macswords, J. , Lee, J. , McLendon, R. and Rich, J.
Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth. ,
PLoS Biol
 8 , e1000319 , 2010
Cells
Glioma stem cells
Enzymes
PDS kit : per instructions
Medium
neurobasal medium
Stem
Human
Human
Cai, L. , Johnstone, B. , Cook, T. , Tan, J. , Fishbein, M. , Chen, P. and March, K.
IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function. ,
Stem Cells
 27 , 230 , 2009
Cells
Adipose stromal
Enzymes
Medium
DMEM
Stem
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
File
10592.pdf (587.72 KB)
Stem
Human
Human
Huang, E. , Hynes, M. , Zhang, T. , Ginestier, C. , Dontu, G. , Appelman, H. , Fields, J. , Wicha, M. and Boman, B.
Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis. ,
Cancer Res
 69 , 3382-9 , 2009
Cells
Colonic epithelial
Enzymes
Medium
not listed
Stem
Human
Human
Varnat, F. , Duquet, A. , Malerba, M. , Zbinden, M. , Mas, C. , Gervaz, P. and Ruiz i Altaba, A.
Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. ,
EMBO Mol Med
 1 , 338-51 , 2009
Cells
Colon cancer
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F12
File
11082.pdf (1.61 MB)
Stem
Human
Human
Zhang, Q. , Yamaza, T. , Kelly, AP, Shi, S. , Wang, S. , Brown, J. , Wang, L. , French, S. , Shi, S. and Le, A.
Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis. ,
PLoS ONE
 4 , e7798 , 2009
Cells
Skin and keloid progenitor
Enzymes
Medium
PBS
File
10991.pdf (1.12 MB)
Stem
Human
Human, 40-65 year
Sun, N. , Panetta, N. , Gupta, D. , Wilson, K. , Lee, A. , Jia, F. , Hu, S. , Cherry, A. , Robbins, R. , Longaker, M. and Wu, J.
Feeder-Free Derivation of Induced Pluripotent Stem Cells from Adult Human Adipose Stem Cells. ,
Proc Natl Acad Sci U S A
 106 , 15720 , 2009
Cells
Adult human adipose stem cells
Enzymes
Medium
DMEM
Stem
Human
Human
Varghese, M. , Olstorn, H. , Berg-Johnsen, J. , Moe, M. , Murrell, W. and Langmoen, I.
Isolation of Human Multipotent Neural Progenitors from Adult Filum Terminale. ,
Stem Cells Dev
 18 , 603 , 2009
Cells
Filum terminale neural progenitor
Enzymes
Trypsin : see reference
Medium
DMEM/F-12
Stem
Human
Human
Pilgaard, L. , Lund, P. , Rasmussen, J. , Fink, T.. and Zachar, V.
Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells. ,
Regen Med
 3 , 705-15 , 2008
Cells
Stem
Enzymes
Collagenase : 280 u/ml
Medium
D-PBS
File
11261.pdf (413.9 KB)
Stem
Human
Human
Secco, M. , Zucconi, E. , Vieira, N. , Fogaca, L.,Cerqueira, A. , Carvalho, M. , Jazedje, T. , Okamoto, O. , Muotri, A. and Zatz, M.
Multipotent Stem Cells from Umbilical Cord: Cord is Richer Than Blood! ,
Stem Cells
 26 , 146 , 2008
Cells
Umbilical mesenchymal stem
Enzymes
Collagenase : 0.1%
Medium
DMEM
File
11075.pdf (1.5 MB)
Stem
Human
Human
Wauthier, E. , Schmelzer, E. , Turner, W. , Zhang, L. , LeCluyse, E. , Ruiz, J. , Turner, R. , Furth, M. , Kubota, H. , Lozoya, O. , Barbier, C. , McClelland, R. , Yao, H. , Moss, N. , Bruce, A. , Ludlow, J. and Reid, L.
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance. ,
Methods Cell Biol
 86 , 137 , 2008
Cells
Hepatic stem cells and heptoblasts
Enzymes
Collagenase Type 4 : 0.014-0.06%
Medium
various
Stem
Human
Human, male
Kossack, N. , Meneses, J. , Shefi, S. , Nguyen, H. , Chavez, S. , Nicholas, C. , Gromoll, J. , Turek, P. and Reijo-Pera, R.
Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells. ,
Stem Cells
 , 2008
Cells
Spermatogonial stem cells
Enzymes
Medium
DMEM
Stem
Human
Human
Nesti, L. , Jackson, W. , Shanti, R. , Koehler, S. , Aragon, A. , Bailey, J. , Sracic, M. , Freedman, B. , Giuliani, J. and Tuan, R.
Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue. ,
J Bone Joint Surg Am
 90 , 2390 , 2008
Cells
Muscle derived multiprogenitor cells
Enzymes
Medium
DMEM
Stem
Human
Human
Jeong, J.
Adipose Stem Cells as a Clinically Available and Effective Source of Adult Stem Cell Therapy ,
Int J Stem Cells
 1 , 43 , 2008
Cells
Adipose derived stem cells
Enzymes
Medium
DMEM
Stem
Human
Human
Kilroy, G. , Foster, S. , Wu, X. , Ruiz, J. , Sherwood, S. , Heifetz, A. , Ludlow, J. , Stricker, D. , Potiny, S. , Green, P. , Halvorsen, Y. , Cheatham, B. , Storms, R. and Gimble, J.
Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors. ,
J Cell Physiol
 212 , 702-9 , 2007
Cells
Adipose derived stem cells
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Nakamura, T. , Endo, K. and Kinoshita, S.
Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling. ,
Stem Cells
 25 , 628-38 , 2007
Cells
Oral epithelial
Enzymes
Neutral Protease : 1.2 u/ml
Trypsin : 0.05%
Medium
PBS
File
11487.pdf (3.01 MB)
Stem
Human
Human
Steigman, S. and Fauza, D.
Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1E.2 , 2007
Cells
Mesenchymal stem
Enzymes
Neutral Protease : see reference
Medium
CMF-DPBS
Stem
Human
Human
Welter, J. and Solchaga, L.and Penick, K.
Simplification of Aggregate Culture of Human Mesenchymal Stem Cells as a Chondrogenic Screening Assay ,
Biotechniques
 42 , 732 , 2007
Cells
Bone marrow derived MSC
Enzymes
Trypsin : 0.05%
Papain : 0.0025%
Medium
DMEM
Stem
Human
Human, 8-12 year
Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F.
Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche ,
Nat Med
 13 , 1219-27 , 2007
Cells
Tendon stem/progenitor
Enzymes
Medium
DMEM
Stem
Human
Human
Li, C. , Heidt, D. , Dalerba, P. , Burant, C. , Zhang, L. , Adsay, V. , Wicha, M. , Clarke, M. and Simeone, D.
Identification of Pancreatic Cancer Stem Cells. ,
Cancer Res
 67 , 1030 , 2007
Cells
Pancreatic cancer stem cells
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
medium 199

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.