For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Adipose/Fat
Mouse

Mouse, Adult

Hay, A. , Murphy, T. , Popichak, K. , Zabel, M. and Moreno, J.
Adipose-derived mesenchymal stromal cells decrease prion-induced glial inflammation in vitro. ,
Sci Rep
 12 , 22567 , 2022
Cells
Mesenchymal stromal
Enzymes
Stemxyme 1 : 400u/ml
Medium
DMEM/F12
Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Colon
Human
Human
Hisamatsu, T. , Watanabe, M. , Ogata, H. , Ezaki, T. , Hozawa, S. , Ishii, H. , Kanai, T. and Hibi, T.
Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis. ,
Cancer Res
 59 , 5927-31 , 1999
Cells
Epithelial and mucosal lymphocytes
Enzymes
CLSPA : 0.02%
Medium
RPMI 1640
Endothelial
Mouse
Mouse, 2 month
Sabbagh, M. and Nathans, J.
A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture ,
Elife
 , 1 , 2020
Cells
Endothelial, CNS
Enzymes
PDS kit : with modifications
Stemxyme 1 : 0.1%
Medium
EC growth medium
Eye
Mouse
Mouse
Schmidt, T. and Kofuji, P.
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells. ,
J Vis Exp
 47 , 2367 , 2011
Cells
Retinal ganglion
Enzymes
CLSPA : 240 u/ml
Hyaluronidase : 0.2%
Medium
Ames
Heart
Mouse

Mouse, neonatal

Angelini, A. , Pi, X. and Xie, L.
Evaluation of Long-Chain Fatty Acid Respiration in Neonatal Mouse Cardiomyocytes using SeaHorse Instrument. ,
Star Protocols
 3 , 101392 , 2022
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.05%
CLSPA : 150 u/ml
Medium
L-15
Heart
Rat
Rat, SD, adult female
Puri S, Bansal DD, Uskokovic MR. and MacGregor RR.
Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) ,
Am J Physiol/Endo
 278(2) , E293 , 2000
Cells
Heart microvascular cells
Enzymes
CLSPA : 250u/ml
Papain : 5 u/ml
Elastase : 0.8 u/ml
Medium
L-15
Intestine
Human
Human
Trapecar, M. , Khan, S. , Roan, N. , Chen, T. , Telwatte, S. , Deswal, M. , Pao, M. , Somsouk, M. , Deeks, S. , Hunt, P. , Yukl, S. and Sanjabi, S.
An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies. ,
AIDS Res Hum Retroviruses
 33 , S31-S39 , 2017
Cells
Lymphocytes
Enzymes
CLSPA : 0.05%
Deoxyribonuclease I : 0.00075%
Medium
RPMI-1640
Miscellaneous
Human
Human
Stern MH, Dreizen S, Mackler BF, Levy BM.
Isolation and characterization of inflammatory cells from the human periapical granuloma ,
J Dent Res
 61 , 1408-12 , 1982
Cells
Periapical granuloma
Enzymes
CLSPA : 0.25%
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse
Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Neural
Human

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Monkey
Macaque
Kupari, J. , Usoskin, D. , Parisien, M. , Lou, D. , Hu, Y. , Fatt, M. , Lonnerberg, P. , Spangberg, M. , Eriksson, B. , Barkas, N. , Kharchenko, P. , Lore, K, Khoury, S. , Diatchenko, L. and Ernfors, P.
Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain. ,
Nat Commun
 12 , 1510 , 2021
Cells
Dorsal root ganglia
Enzymes
Papain : 35 u/ml
Stemxyme 1 : 0.2%
Medium
see reference
Neural
Mouse

Mouse, 4-6 week

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Mouse

Mouse

Yousuf, M. , Moreno, M. , Li, J. , He, L. , Royer, D. , Zhang, J. , Woodall, B. , Grace, P. and Price, T.
Diroximel fumarate acts hrough Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons. ,
bioRxivorg
 Version 1 , Preprint Dec 23 , 2023
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Mouse
Mouse
Yousuf, M. , Samtleben, S. , Lamothe, S. , Friedman, T. , Catuneanu, A. and et al.
ER Stress in the Dorsal Root Ganglia Regulates BK Channel Physiology and Contributes to Pain Hypersensitivity in a Mouse Model of Multiple Schlerosis ,
bioRxivorg
 , 2020
Cells
Dorsal root ganglion
Enzymes
Stemxyme 1 : 0.1-0.2%
Medium
HBSS
Neural
Rat
Rat, adult
George, D. , Ahrens, P. and Lambert, S.
Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells. ,
Glia
 , 2018
Cells
Schwann
Enzymes
Stemxyme 1 : 0.05%
Medium
DMEM
Organoids
Mouse

Mouse

Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, adult
Voronina, S. , Barrow, S. , Gerasimenko, O. , Petersen, O. and Tepikin, A.
Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi. ,
J Biol Chem
 279 , 27327 , 2004
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
see reference
Pancreas
Mouse
Mouse, albino, 25 g
Fogarty, K. , Kidd, J. , Tuft, R. and Thorn, P.
A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells. ,
Biophys J
 78 , 2298 , 2000
Cells
Acinar
Enzymes
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse
Toivola, D. , Ku, N. , Ghori, N. , Lowe, A. , Michie, S. and Omary, M.
Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury. ,
Exp Cell Res
 255 , 156 , 2000
Cells
Acinar cells and acini
Enzymes
Collagenase Type 1 : see reference
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse, male, 18-24 g
Burnham DB, Williams JA.
Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini ,
J Biol Chem
 257 , 10523-8 , 1982
Cells
Acinar
Enzymes
CLSPA : 70-90 u/ml
Medium
Krebs-Henseleit
Pancreas
Rat
Rat
Williams, J.
Isolation of Rodent Pancreatic Acinar Cells and Acini by Collagenase Digestion ,
Pancreapedia
 , 2010
Cells
Acinar
Enzymes
CLSPA : 100 u/ml
Medium
DMEM
Pancreas
Rat
Rat, SD, male
Ji B, Kopin AS, Logsdon CD.
Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180 ,
J Biol Chem
 275 , 19115-20 , 2000
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Rat
Rat
Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS.
Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase ,
Am J Physiol Cell Physiol
 279 , C1993-2003 , 2000
Cells
Pancreatic acini
Enzymes
CLSPA : 30 u/ml
Collagenase Type 4 : 30 u/ml
Medium
M199
Stem
Mouse
Mouse, xenograft
Pham, K. , Delitto, D. , Knowlton, A. , Hartlage, E. , Madhavan, R. , Gonzalo, D. , Thomas, R. , Behrns, K. , George, T. , Hughes, S. , Wallet, S. , Liu, C. and Trevino, J.
Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. ,
Am J Pathol
 186 , 2016
Cells
Pancreatic cancer
Enzymes
Stemxyme 1 : 0.2%
Medium
HBSS
Tumor
Human
Human
Pham, K. , Delitto, D. , Knowlton, A. , Hartlage, E. , Madhavan, R. , Gonzalo, D. , Thomas, R. , Behrns, K. , George, T. , Hughes, S. , Wallet, S. , Liu, C. and Trevino, J.
Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. ,
Am J Pathol
 186 , 2016
Cells
Pancreatic cancer
Enzymes
Stemxyme 1 : 0.2%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.