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Tissue References Search Tool

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Neural
Mouse
Mouse, adult
Yip, P. , Kaan, T. , Fenesan, D. and Malcangio, M.
Rapid Isolation and Culture of Primary Microglia from Adult Mouse Spinal Cord. ,
J Neurosci Methods
 183 , 223-37 , 2009
Cells
Spinal microganglia
Enzymes
Papain : 0.2%
Medium
Hibernate A
Neural
Mouse
Mouse, neonatal
Pedrola, L. , Espert, A. , Valdes-Sanchez, T. , Sanchez-Piris, M. , Sirkowski, E. , Scherer, S. , Farinas, I. and Palau, F.
Cell Expression of GDAP1 in the Nervous System and Pathogenesis of Charcot-Marie-Tooth Type 4A Disease. ,
J Cell Mol Med
 12 , 679 , 2008
Cells
DRG neurons
Enzymes
Collagenase : 0.2%
Trypsin : 0.05%
Medium
Ham's F12
Neural
Mouse
Mouse, 6 mo
Brewer Gregory J, Torricelli John R.
Isolation and culture of adult neurons and neurospheres ,
Nat Protoc
 2 , 1490-8 , 2007
Cells
Neurons, neurospheres
Enzymes
Papain : 0.2%
Medium
Hibernate
Neural
Mouse
Mouse, 6-8 week
Malin, S. , Davis, B. and Molliver, D.
Production of Dissociated Sensory Neuron Cultures and Considerations for their use in Studying Neuronal Function and Plasticity ,
Nat Protoc
 2 , 152 , 2007
Cells
Sensory neurons, DRG
Enzymes
Papain : 20 u/ml
Medium
HBSS
Neural
Mouse
Mouse
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Mouse
Mouse, C57BL/6, 1 year
Eide, L. and McMurray, C
Culture of Adult Mouse Neurons ,
Biotechniques
 38(1) , 99-104 , 2005
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
DMEM
Neural
Mouse
Mouse, C57BL
Okano-Uchida T, Himi T, Komiya Y. and Ishizaki Y
Cerebellar granule cell precursors can differentiate into astroglial cells ,
Proc Natl Acad Sci U S A
 101 , 1211 , 2004
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 16.5 u/ml
Medium
Dulbecco's PBS
Neural
Mouse
Mouse, 1-3 day
Savchenko V, Sung U, Blakely RD.
Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody ,
Mol Cell Neurosci
 24 , 1131 , 2003
Cells
Neurons, ganglia
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 100 u/ml
Collagenase : 0.3%
Trypsin : 0.05%
Medium
HBSS
Neural
Mouse
Mouse, male
Roberts, L. and MacDonald, C.and Mark, C.
Anandamide is a Partial Agonist at Native Vanilloid Receptors in Acutely Isolated Mouse Trigeminal Sensory Neurons. ,
Br J Pharmacol
 137 , 421 , 2002
Cells
Trigeminal senesory neurons
Enzymes
Papain : 20 u/ml
Medium
HEPES buffered saline
Neural
Mouse
Mouse (BALB/c), adult
Eagleson, K. and Bennett, M.
Survival of Purified Motor Neurons In Vitro: Effects of Skeletal Muscle-Conditioned Medium ,
Neurosci Lett
 38 , 187 , 1983
Cells
Neurons, spinal cord
Enzymes
Medium
Hank's BSS, CMF
Neural
Porcine
Porcine, adult, 60-100 kg
Si ML, Lee TJ.
Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries ,
J Pharmacol Exp Ther
 298 , 122 , 2001
Cells
Superior cervical ganglia
Enzymes
Papain : 2 u/ml
Collagenase : 0.12%
Medium
HBSS
Neural
Rat
Rat, 2-3 week
Lin, Y. and Chen, J.
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release. ,
J Vis Exp
 , 2018
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Rat
Rat, SD, 3 month
Andersen, N. and Monje, P.
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers. ,
Methods Mol Biol
 1739 , 49-66 , 2018
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, SD, day 2
Maurel, P.
Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies. ,
Methods Mol Biol
 1739 , 17-37 , 2018
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Rat
Rat, Wistar, 3 day
Fangmann, L. , Teller, S. , Stupakov, P. , Friess, H. , Ceyhan, G. and Demir, I.
3D Cancer Migration Assay with Schwann Cells. ,
Methods Mol Biol
 1739 , 317-325 , 2018
Cells
Dorsal root ganglia neurons
Enzymes
Medium
MEM
Neural
Rat
Rat, adult
George, D. , Ahrens, P. and Lambert, S.
Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells. ,
Glia
 , 2018
Cells
Schwann
Enzymes
Stemxyme 1 : 0.05%
Medium
DMEM
Neural
Rat
Rat, SD, p7
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, SD
Andersen, N. , Srinivas, S. , Pinero, G. and Monje, P.
A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves. ,
Sci Rep
 6 , 31781 , 2016
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, SD
Schuh, C. , Hercher, D. , Stainer, M. , Hopf, R. , Teuschl, A. , Schmidhammer, R. and Redl, H.
Extracorporeal Shockwave Treatment: A Novel Tool to Improve Schwann Cell Isolation and Culture. ,
Cytotherapy
 18 , 760-70 , 2016
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, embryonic
Oyanagi, K. , Tashiro, T. and Negishi, T.
Cell-Type-Specific and Differentiation-Status-Dependent Variations in Cytotoxicity of Tributyltin in Cultured Rat Cerebral Neurons and Astrocytes. ,
J Toxicol Sci
 40 , 459-68 , 2015
Cells
Cerebral neurons
Enzymes
Papain : 2 u/ml
Medium
DMEM-PBS
Neural
Rat
Rat, SD, 6-9 week
Murayama, C. , Watanabe, S. and Nakamura, M. and, Norimoto, H.
Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons. ,
Molecules
 20 , 14959-69 , 2015
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Rat
Rat, embryonic and neonatal
Rheaume, C. , Cai, B. , Wang, J. , Fernandez-Salas, E. , Aoki, K. , Francis, J. and Broide, R.
A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25. ,
Toxins
 7 , 2354-70 , 2015
Cells
Cortical neurons, DRG
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Neural
Rat
Rat
Yu, R. , Seymour, V. , Berecki, G. , Jia, X. , Akca, M. , Adams, D. , Kaas, Q. and Craik, D.
Less is More: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic α-Conotoxin Vc1.1. ,
Sci Rep
 5 , 13264 , 2015
Cells
Dorsal root ganglion neurons
Enzymes
Medium
HBSS
Neural
Rat
Rat, SD
Wei, X. , Yan, J. , Tillu, D. , Asiedu, M. , Weinstein, N. , Melemedjian, O. , Price, T. and Dussor, G.
Meningeal Norepinephrine Produces Headache Behaviors in Rats via Actions Both on Dural Afferents and Fibroblasts. ,
Cephalalgia
 35 , 1054-64 , 2015
Cells
Trigeminal ganglion
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Neural
Rat
Rat, neonatal
Stettner, M. , Lohmann, B. , Wolffram, K. , Weinberger, J. , Dehmel, T. , Hartung, H. , Mausberg, A. and Kieseier, B.
Interleukin-17 Impedes Schwann Cell-Mediated Myelination. ,
J Neuroinflammation
 11 , 63 , 2014
Cells
Schwann cells
Enzymes
Collagenase : 0.05-0.1%
Trypsin : 0.125-0.25%
Medium
DMEM
Neural
Rat
Rat, 1-3 day
Thurner, P. , Gsandtner, I. , Kudlacek, O. , Choquet, D. , Nanoff, C. , Freissmuth, M. and Zezula, J.
A Two-State Model for the Diffusion of the A2A Adenosine Receptor in Hippocampal Neurons: Agonist-Induced Switch to Slow Mobility is Modified by Synapse-Associated Protein 102 (SAP102). ,
J Biol Chem
 289 , 9263-74 , 2014
Cells
Hippocampal neurons
Enzymes
Papain : 25 u/ml
Medium
L-15
Neural
Rat
Rat, E18
Todd, G. , Boosalis, C. , Burzycki, A. , Steinman, M. , Hester, L. , Shuster, P. and Patterson, R.
Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons. ,
PLoS ONE
 8 , e58996 , 2013
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Neural
Rat
Rat, SD, 175-200 g
Yan, J. , Melemedjian, O. , Price, T. and Dussor, G.
Sensitization of Dural Afferents Underlies Migraine-Related Behavior Following Meningeal Application of Interleukin-6 (IL-6). ,
Mol Pain
 8 , 6 , 2012
Cells
Trigeminal ganglia
Enzymes
Papain : 20 u/ml
Medium
HBSS
Neural
Rat
Rat, E19-21 and P1-3
Ghogha, A. , Bruun, D. and Lein, P.
Inducing Dendritic Growth in Cultured Sympathetic Neurons. ,
J Vis Exp
 61 , e3546 , 2012
Cells
Sympathetic neurons
Enzymes
Medium
DMEM/F-12
Neural
Rat
Rat, Wistar, E18
Giacomello, M. , Girardi, S. , Scorzeto, M. , Peruffo, A. , Maschietto, M. , Cozzi, B. and Vassanelli, S.
Stimulation of Ca²+ Signals in Neurons by Electrically Coupled Electrolyte-Oxide-Semiconductor Capacitors. ,
J Neurosci Methods
 198 , 1 , 2011
Cells
Neurons
Enzymes
Papain : 0.05%
Medium
Neurobasal
Neural
Rat
Rat, 20-30 day
Bosmans, F. , Puopolo, M. , Martin-Eauclaire, M. , Bean, B. and Swartz, K.
Functional Properties and Toxin Pharmacology of a Dorsal Root Ganglion Sodium Channel Viewed through its Voltage Sensors. ,
J Gen Physiol
 138 , 59 , 2011
Cells
Dorsal root ganglion
Enzymes
Papain : 20 u/ml
Medium
L-15
Neural
Rat
Rat, SD, E18
Peltier, D. , Simms, A. , Farmer, J. and Miller, D.
Human Neuronal Cells Possess Functional Cytoplasmic and TLR-Mediated Innate Immune Pathways Influenced by Phosphatidylinositol-3-Kinase Signaling ,
J Immunol
 184 , 7010 , 2010
Cells
Neuronal
Enzymes
Papain : 0.2%
Medium
Neurobasal E
Neural
Rat
Rat, SD, 200-300 g
East, E. , de Oliveira, D. , Golding, J. and Phillips, J.
Alignment of Astrocytes Increases Neuronal Growth in Three-Dimensional Collagen Gels and is Maintained Following Plastic Compression to Form a Spinal Cord Repair Conduit. ,
Tissue Eng Part A
 16 , 3173 , 2010
Cells
Dorsal root ganglia, fibroblast
Enzymes
Collagenase : 0.125%
Medium
DMEM
Neural
Rat
Rat, Fisher, 7-21 month
Chen, N. , Newcomb, J. , Garbuzova-Davis, S. , Davis Sanberg, C. , Sanberg, P. and Willing, A.
Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro. ,
Aging Dis
 1 , 173 , 2010
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
Hibernate A
Neural
Rat
Rat, SD, embryonic day 18
Liu, Y. , Yohrling, G. , Wang, Y. , Hutchinson, T. , Brenneman, D. , Flores, C. and Zhao, B.
Carisbamate, a Novel Neuromodulator, Inhibits Voltage-Gated Sodium Channels and Action Potential Firing of Rat Hippocampal Neurons. ,
Epilepsy Res
 83 , 66 , 2009
Cells
Hippocampal neurons
Enzymes
Papain : 20 u/ml
Medium
Neurobasal/B27

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.