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Tissue References Search Tool

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Neural
Rat
Rat, SD, day 2
Maurel, P.
Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies. ,
Methods Mol Biol
 1739 , 17-37 , 2018
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Rat
Rat, Wistar, 3 day
Fangmann, L. , Teller, S. , Stupakov, P. , Friess, H. , Ceyhan, G. and Demir, I.
3D Cancer Migration Assay with Schwann Cells. ,
Methods Mol Biol
 1739 , 317-325 , 2018
Cells
Dorsal root ganglia neurons
Enzymes
Medium
MEM
Neural
Rat
Rat, adult
George, D. , Ahrens, P. and Lambert, S.
Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells. ,
Glia
 , 2018
Cells
Schwann
Enzymes
Stemxyme 1 : 0.05%
Medium
DMEM
Neural
Rat
Rat, SD, p7
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, SD
Andersen, N. , Srinivas, S. , Pinero, G. and Monje, P.
A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves. ,
Sci Rep
 6 , 31781 , 2016
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, SD
Schuh, C. , Hercher, D. , Stainer, M. , Hopf, R. , Teuschl, A. , Schmidhammer, R. and Redl, H.
Extracorporeal Shockwave Treatment: A Novel Tool to Improve Schwann Cell Isolation and Culture. ,
Cytotherapy
 18 , 760-70 , 2016
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, SD, 6-9 week
Murayama, C. , Watanabe, S. and Nakamura, M. and, Norimoto, H.
Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons. ,
Molecules
 20 , 14959-69 , 2015
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Rat
Rat, embryonic and neonatal
Rheaume, C. , Cai, B. , Wang, J. , Fernandez-Salas, E. , Aoki, K. , Francis, J. and Broide, R.
A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25. ,
Toxins
 7 , 2354-70 , 2015
Cells
Cortical neurons, DRG
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Neural
Rat
Rat
Yu, R. , Seymour, V. , Berecki, G. , Jia, X. , Akca, M. , Adams, D. , Kaas, Q. and Craik, D.
Less is More: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic α-Conotoxin Vc1.1. ,
Sci Rep
 5 , 13264 , 2015
Cells
Dorsal root ganglion neurons
Enzymes
Medium
HBSS
Neural
Rat
Rat, SD
Wei, X. , Yan, J. , Tillu, D. , Asiedu, M. , Weinstein, N. , Melemedjian, O. , Price, T. and Dussor, G.
Meningeal Norepinephrine Produces Headache Behaviors in Rats via Actions Both on Dural Afferents and Fibroblasts. ,
Cephalalgia
 35 , 1054-64 , 2015
Cells
Trigeminal ganglion
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Neural
Rat
Rat, neonatal
Stettner, M. , Lohmann, B. , Wolffram, K. , Weinberger, J. , Dehmel, T. , Hartung, H. , Mausberg, A. and Kieseier, B.
Interleukin-17 Impedes Schwann Cell-Mediated Myelination. ,
J Neuroinflammation
 11 , 63 , 2014
Cells
Schwann cells
Enzymes
Collagenase : 0.05-0.1%
Trypsin : 0.125-0.25%
Medium
DMEM
Neural
Rat
Rat, SD, 175-200 g
Yan, J. , Melemedjian, O. , Price, T. and Dussor, G.
Sensitization of Dural Afferents Underlies Migraine-Related Behavior Following Meningeal Application of Interleukin-6 (IL-6). ,
Mol Pain
 8 , 6 , 2012
Cells
Trigeminal ganglia
Enzymes
Papain : 20 u/ml
Medium
HBSS
Neural
Rat
Rat, E19-21 and P1-3
Ghogha, A. , Bruun, D. and Lein, P.
Inducing Dendritic Growth in Cultured Sympathetic Neurons. ,
J Vis Exp
 61 , e3546 , 2012
Cells
Sympathetic neurons
Enzymes
Medium
DMEM/F-12
Neural
Rat
Rat, 20-30 day
Bosmans, F. , Puopolo, M. , Martin-Eauclaire, M. , Bean, B. and Swartz, K.
Functional Properties and Toxin Pharmacology of a Dorsal Root Ganglion Sodium Channel Viewed through its Voltage Sensors. ,
J Gen Physiol
 138 , 59 , 2011
Cells
Dorsal root ganglion
Enzymes
Papain : 20 u/ml
Medium
L-15
Neural
Rat
Rat, SD, 200-300 g
East, E. , de Oliveira, D. , Golding, J. and Phillips, J.
Alignment of Astrocytes Increases Neuronal Growth in Three-Dimensional Collagen Gels and is Maintained Following Plastic Compression to Form a Spinal Cord Repair Conduit. ,
Tissue Eng Part A
 16 , 3173 , 2010
Cells
Dorsal root ganglia, fibroblast
Enzymes
Collagenase : 0.125%
Medium
DMEM
Neural
Rat
Rat, Wistar, 7 day
Sakisaka, T. , Yamamoto, Y. , Mochida, S. , Nakamura, M. , Nishikawa, K. , Ishizaki, H. , Okamoto-Tanaka, M. , Miyoshi, J. , Fujiyoshi, Y. , Manabe, T. and Takai, Y.
Dual Inhibition of SNARE Complex Formation by Tomosyn Ensures Controlled Neurotransmitter Release. ,
J Cell Biol
 183 , 323 , 2008
Cells
Superior cervical ganglion
Enzymes
Collagenase : 0.05%
Medium
L-15
Neural
Rat
Rat, SD, male, 5-8 week
Connor, M, Naves, L. and McCleskey, E.
Contrasting Phenotypes of Putative Proprioceptive and Nociceptive Trigeminal Neurons Innervating Jaw Muscle in Rat. ,
Mol Pain
 1 , 31 , 2005
Cells
Trigeminal neurons
Enzymes
Papain : 20 u/ml
Collagenase : 0.3%
Medium
CMF Hanks
Neural
Rat
Rat, SD, 0-2 day
Pedraza Carlos E, Podlesniy Petar, Vidal Noemi­, Arevalo Juan Carlos, Lee Ramee, Hempstead Barbara, Ferrer Isidre, Iglesias Montse, Espinet Carme.
Pro-NGF isolated from the human brain affected by Alzheimer's disease induces neuronal apoptosis mediated by p75NTR ,
Am J Pathol
 166 , 533-43 , 2005
Cells
Superior cervical ganglion
Enzymes
Collagenase Type 4 : 20 u/ml
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, Wistar, E15
Moriya-Ito K, Osada T, Ishimatsu Y, Muramoto K, Kobayashi T, Ichikawa M.
Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons ,
Chem Senses
 30 , 111 , 2005
Cells
Vomeronasal receptor neurons
Enzymes
Papain : 0.5 u/ml
Medium
DMEM/F12
Neural
Rat
Rat, SD, male 150-220 g
Hu Hong-Zhen, Gu Qihai, Wang Chunbo, Colton Craig K, Tang Jisen, Kinoshita-Kawada Mariko, Lee Lu-Yuan, Wood Jackie D, Zhu Michael X.
2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3 ,
J Biol Chem
 279 , 35741-8 , 2004
Cells
Dorsal root ganglion neurons
Enzymes
Trypsin : 0.05%
Medium
DMEM/Ham's F12
Neural
Rat
Rat, SD, embryonic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
Neural
Rat
Rat, SD, female, pregnant
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
Neural
Rat
Rat, SD, embryonic
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
Neural
Rat
Rat, 1-6 day
Robertson, S. , Rae, M. , Rowan, E. and Kennedy, C.
Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia. ,
Br J Pharmacol
 118 , 951 , 1996
Cells
DRG neurons
Enzymes
Papain : 20 u/ml
Collagenase Type 1 : 150 u/ml
Medium
HBSS
Neural
Rat
Rat
Stemple, D. and Anderson, D.
Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest ,
Cell
 71 , 973 , 1992
Cells
Stem, neural crest
Enzymes
Collagenase : 0.075%
Medium
Ringer's solution
Neural
Rat
Rat, newborn
Reichardt, L. and Patterson, P.
Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures ,
Nature
 270 , 147 , 1977
Cells
Neurons, sympathetic
Enzymes
Medium
Hank's solution, CF
Neural
Shellfish
Mollusc, juvenile 1-2 gm or adult 50-100 gm
Schacher, S. and Proshanksy, E.
Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment ,
J Neurosci
 3 (12) , 2403 , 1983
Cells
Neurons
LUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Neural
Shellfish
Aplysia californica
Camardo, J. , Proshansky, E. and Schacher, S.
Identified Aplysia Neurons Form Specific Chemical Synapses in Culture ,
J Neurosci
 3 (12) , 2614 , 1983
Cells
LUQ cells
RUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Organoids
Human

Human

Gamero-Estevez, E. , Hensel, I. , Steinhauer, M. , Müllertz, O. , Savochkina, E. , Sektioglu, I. , Stoll, B. , Derakhshani, S. , Devriese, S. , Kim, K. and Resnik-Docampo, M.
Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine. ,
Bio Protoc
 15 , e5157 , 2025
Cells
Intestinal organoids
Enzymes
Medium
RPMI
Organoids
Human

Human

Chiu, M. , Li, C. , Yu, Y. , Liu, X. , Huang, J. , Wan, Z. , Yuen, K. and Zhou, J.
Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids. ,
Bio Protoc
 13 , e4657 , 2023
Cells
Lung Organoids
Enzymes
Medium
Basal medium
Organoids
Human

Human, adolescent

Hemeryck, L. , Hermans, F. , Chappell, J. , Kobayashi, H. , Lambrechts, D. , Lambrichts, I. , Bronckaers, A. and Vankelecom, H.
Organoids from Human Tooth Showing Epithelial Stemness Phenotype and Differentiation Potential. ,
Cell Mol Life Sci
 79 , 153 , 2022
Cells
Epithelial organoids
Enzymes
Medium
MEM
Organoids
Human

Human

Vaart, J. , Böttinger, L. , Geurts, M. , Wetering, W. , Knoops, K. , Sachs, N. , Begthel, H. , Korving, J. , Lopez-Iglesias, C. , Peters, P. , Eitan, K. , Gileles-Hillel, A. and Clevers, H.
Modelling of Primary Ciliary Dyskinesia using Patient-derived Airway Organoids. , EMBO Rep Vol. 22 , e52058 , 2021
Cells
Airway organoids
Enzymes
Pronase : 0.15%
Collagenase : 0.05%
Medium
Airway medium
Organoids
Human

Human

Marinucci, M. , Ercan, C. , Taha-Mehlitz, S. , Fourie, L. , Panebianco, F. , Bianco, G. , Gallon, J. , Staubli, S. , Soysal, S. , Zettl, A. , Rauthe, S. , Vosbeck, J. , Droeser, R. , Bolli, M. , Peterli, R. , Flüe, M. , Ng, C. , Kollmar, O. , Coto-Llerena, M. and Piscuoglio, S.
Standardizing Patient-Derived Organoid Generation Workflow to Avoid Microbial Contamination From Colorectal Cancer Tissues. , Front Oncol Vol. 11 , 781833 , 2021
Cells
Colorectal cancer organoids
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Schreurs, R. , Baumdick, M. , Drewniak, A. and Bunders, M.
Co-culture of Human Intestinal Organoids and Lamina Propria-derived CD4 T Cells. , STAR Protoc Vol. 2 , 100519 , 2021
Cells
Lamina propria lymphocytes
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Mansour, A. , Gonçalves, J. , Bloyd, C. , Li, H. , Fernandes, S. , Quang, D. , Johnston, S. , Parylak, S. , Jin, X. and Gage, F.
An In Vivo Model of Functional and Vascularized Human Brain Organoids. ,
Nat Biotechnol
 36 , 432-441 , 2018
Cells
Cerebral organoids
Enzymes
Medium
DMEM/F12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.