For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Adipose/Fat
Mouse

Mouse, Adult

Hay, A. , Murphy, T. , Popichak, K. , Zabel, M. and Moreno, J.
Adipose-derived mesenchymal stromal cells decrease prion-induced glial inflammation in vitro. ,
Sci Rep
 12 , 22567 , 2022
Cells
Mesenchymal stromal
Enzymes
Stemxyme 1 : 400u/ml
Medium
DMEM/F12
Adrenal
Mouse
Mouse
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Bone
Mouse
Mouse
Lee, D. , Choung, H. , Kim, H. , Gronostajski, R. , Yang, Y. , Ryoo, H. , Lee, Z. , Kim, H. , Cho, E. and Park, J.
NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. ,
Stem Cells
 32 , 2467-79 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Brain
Mouse
Adult and neonatal
Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P.
Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Cells
Microglia, astrocytes, lymphocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Papain : 20 u/ml
Neutral Protease : 1.2 u/ml
Medium
EBSS
Brain
Mouse
Mouse
Kramer, D. , Risso, D. , Kosillo,. , Ngai, J. and Bateup, H.
Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability , eNeuro Vol. 5 , 1 , 2018
Cells
Neurons
Enzymes
Papain : 10 u/ml
Medium
Artificial CSF
Brain
Mouse
Mouse, P13-15
Haseleu, J. , Anlauf, E. , Blaess, S. , Endl, E. and Derouiche, A.
Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs). ,
Front Cell Neurosci
 7 , 54 , 2013
Cells
Glial
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Mouse
Mouse, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
Brain
Mouse
Mouse
Shah. G. , Price, T. , Banks, W. , Morofuji, Y. , Kovac, A. , Ercal, N. , Sorenson, C. , Shin, E. and Sheibani, N.
Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis. ,
J Pharmacol Exp Ther
 344 , 637-45 , 2013
Cells
Cerebral pericytes
Enzymes
Medium
DMEM
Brain
Mouse
Mouse
Ryg-Cornejo, V. , Ioannidis, L. and Hansen, D.
Isolation and Analysis of Brain-Sequestered Leukocytes from Plasmodium Berghei ANKA-Infected Mice. ,
J Vis Exp
 71 , e50112 , 2013
Cells
Brain-sequestered leukocytes
Enzymes
Medium
RPMI
Brain
Mouse
Mouse, 1-2 day
O'Meara, R. , Ryan, S. , Colognato, H. and Kothary, R.
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues. ,
J Vis Exp
 54 , 3324 , 2011
Cells
Oligodendrocytes, dorsal root ganglia
Enzymes
Papain : 0.15%
Medium
DMEM
Brain
Mouse
Mouse, postnatal
Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S.
Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. ,
Stem Cells
 26 , 2875 , 2008
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Deoxyribonuclease I : 1,000 u/ml
Medium
HBSS
Brain
Mouse
Mouse
Oliver, T. , Read, T. , Kessler, J. , Mehmeti, A. , Wells, J. , Huynh, T. , Lin, S. and Wechsler-Reya, R.
Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma. ,
Development
 132 , 2425 , 2005
Cells
Granule cell precursors, pre-neoplastic and tumor cells
Enzymes
Papain : 10 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Neurobasal/B27
Brain
Mouse
Mouse, 3 day
Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H.
Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia ,
J Biol Chem
 277 , 4816 , 2002
Cells
Microglia
Enzymes
Papain : 90 u/ml
Deoxyribonuclease I : 2000 u/ml
Medium
Eagle's MEM
Brain
Mouse
Mouse, SD, 8-12 week
O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL.
IGF-I and microglia/macrophage proliferation in the ischemic mouse brain ,
Glia
 39 , 85 , 2002
Cells
Microglia
Enzymes
Medium
RPMI-1640
Brain
Mouse
Mouse
Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S. and Ishizaki Y.
A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation ,
J Neurosci
 20 , 5756 , 2000
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 0.435%
Medium
EBSS
Colon
Mouse
Mouse
Larmonier, C. , Shehab, K. , Laubitz, D. , Jamwal, D. , Ghishan, F. and Kiela, P.
Transcriptional Reprogramming and Resistance to Colonic Mucosal Injury in Poly(ADP-ribose) Polymerase 1 (PARP1)-deficient Mice. ,
J Biol Chem
 291 , 8918-30 , 2016
Cells
Lamina propria
Enzymes
Collagenase : 100 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse
Fattahi, F. , Steinbeck, J. , Kriks, S. , Tchieu, J. , Zimmer, B. , Kishinevsky, S. , Zeltner, N. , Mica, Y. , El-Nachef, W. , Zhao, H. , de Stanchina, E. , Gershon, M. , Grikscheit, T. , Chen, S. and Studer, L.
Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease. ,
Nature
 531 , 105-9 , 2016
Cells
Colon organoid
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Colon
Mouse
Mouse, 8-10 week
Round, J. and Mazmanian, S.
Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota. ,
Proc Natl Acad Sci U S A
 107 , 12204-9 , 2010
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Atarashi, K. , Nishimura, J. , Shima, T. , Umesaki, Y. , Yamamoto, M. , Onoue, M. , Yagita, H. , Ishii, N. , Evans, R. , Honda, K. and Takeda, K.
ATP Drives Lamina Propria T(H)17 Cell Differentiation. ,
Nature
 455 , 808 , 2008
Cells
Lamina propria
Enzymes
Medium
RPMI 1640
Colon
Mouse
Mouse
Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F.
Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue ,
Nat Protoc
 2 , 2307-11 , 2007
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse, 6-8 week
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F.
Phenotypic characterization of human colorectal cancer stem cells ,
Proc Natl Acad Sci U S A
 104 , 10158-63 , 2007
Cells
Cancer stem cell
Enzymes
Collagenase Type 3 : 200 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
RPMI-1640
Colon
Mouse
Mouse, 6-8 week
Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M.
Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis ,
Am J Physiol/Gastro
 284 , G595-603 , 2003
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Endothelial
Mouse
Mouse
Luo, S. , Truong, A. and Makino, A.
Isolation of Mouse Coronary Endothelial Cells. ,
J Vis Exp
 , 2016
Cells
Coronary endothelial
Enzymes
Medium
Kreb's
Endothelial
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.
Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
 3 , 2014
Cells
Cerebral artery endothelial
Enzymes
Neutral Protease : 4 u/ml
Elastase : 1 u/ml
Collagenase Type 1 : 120 u/ml
Medium
DMEM
Endothelial
Mouse
Mouse
Daneman, R. , Zhou, L. , Agalliu, D. , Cahoy, J. , Kaushal, A. and Barres, B.
The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells. ,
PLoS ONE
 5 , e13741 , 2010
Cells
Endothelial
Enzymes
Papain : 40 u/ml
Deoxyribonuclease I : 125 u/ml
Medium
DPBS
Endothelial
Mouse
Mouse, embryonic or yolk sac
Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong.
Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation ,
J Cell Biol
 172 , 151-62 , 2006
Cells
Endothelial
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse, 8-12 week
Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.
Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
 6 , 2020
Cells
Nasal epithelium
Enzymes
Papain : 20 u/ml
Medium
EBSS
Epithelial
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
 446 , 77-106 , 2008
Cells
Epithelial
Enzymes
Collagenase : 0.025%
Medium
DMEM
Epithelial
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.