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Tissue References Search Tool

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Eye
Bovine
Bovine
Terlizzi, V. , Kolibabka, M. , Burgess, J. , Hammes, H. and Harmsen, M.
The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2. ,
Stem Cells
 36 , 240-251 , 2018
Cells
Retinal endothelial
Enzymes
Pronase : 0.02%
Medium
DMEM
Eye
Chicken
Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.
Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
 180 , 159-68 , 2005
Cells
Retinal cells
Enzymes
Trypsin : 0.005%
Medium
DMEM/F12
Eye
Monkey
Macaques and baboon
Han ,Y. , Jacoby, R. and Wu, S.
Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells. ,
Brain Res
 875 , 175 , 2000
Cells
Retinal
Enzymes
Papain : 20-40 u/ml
Deoxyribonuclease I : 400 u/ml
Medium
Ames' solution
Eye
Mouse

Mouse, 2 month

Murenu, E. , Pavlou, M. , Richter, L. , Rapti, K. , Just, S. and Cehajic-Kapetanovic, J.
A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species via Fluorescence-Activated Cell Sorting. , Mol Ther Methods Clin Dev Vol. 20 , 587-600 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Eye
Mouse
Mouse
Ortin-Martinez, A. , Tsai, E. , Nickerson, P. , Bergeret, M. , Lu, Y. , Smiley, S. , Comanita, L. and Wallace, V.
A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors. ,
Stem Cells
 35 , 932-939 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
PBS
Eye
Mouse
Mouse
Wohl, S. , Jorstad, N. , Levine, E. and Reh, T.
Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture. ,
Nat Commun
 8 , 1603 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
HBSS
Eye
Mouse
Mouse, C57BL/6
Blandford, S. and Baldridge, W.
The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i. ,
Biomed Res Int
 2016 , 8178162 , 2016
Cells
Retinal astrocytes
Enzymes
Papain : 16.5 u/ml
Medium
HBSS
Eye
Mouse
Mouse, embryonic and P0
Dvoriantchikova, G. , Perea-Martinez, I. , Pappas, S. , Barry, A. , Danek, D. , Dvoriantchikova, X. , Pelaez, D. and Ivanov, D.
Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina. ,
PLoS ONE
 10 , e0131054 , 2015
Cells
Retinal
Enzymes
Papain : 8 u/ml
Deoxyribonuclease I : 124 u/ml
Medium
DPBS
Eye
Mouse
Mouse
Amadi-Obi, A. , Yu, C. , Liu, X. , Mahdi, R. , Clarke, G. , Nussenblatt, R, Gery, I. , Lee, Y. and Egwuagu, C.
TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1. ,
Nat Med
 13 , 711 , 2007
Cells
Retinal
Enzymes
Medium
RPMI
Eye
Mouse
Mouse, 2-5 month
Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto.
Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission ,
J Neurosci
 25 , 566-76 , 2005
Cells
Mouse retinal and bipolar
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
HBSS
Eye
Rat

Rat, SD, 1-2 day

Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q.
Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. ,
Neuroreport
 34 , 385-394 , 2023
Cells
Retinal ganglion
Enzymes
Papain : 0.5%
Medium
MEM
Eye
Rat
Rat, Wister, 3-30 wk
Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y.
Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. ,
PLoS ONE
 11 , e0155468 , 2016
Cells
Retinal
Enzymes
Papain : 30 u/ml
Medium
EBSS
Neural
Chicken
Chick, White Leghorn, embryos
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Neural
Human

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Human
Human, 26 week
Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.
Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells ,
J Neurosci Res
 75 , 782 , 2004
Cells
Neural progenitor cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Neural
Human
Human, adult
Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA.
Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone ,
J Neurosci Res
 59 , 321 , 2000
Cells
Ventricular epithelial
Enzymes
Papain : 11.4 u/ml
Medium
DMEM/F12
Neural
Monkey
Macaque
Kupari, J. , Usoskin, D. , Parisien, M. , Lou, D. , Hu, Y. , Fatt, M. , Lonnerberg, P. , Spangberg, M. , Eriksson, B. , Barkas, N. , Kharchenko, P. , Lore, K, Khoury, S. , Diatchenko, L. and Ernfors, P.
Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain. ,
Nat Commun
 12 , 1510 , 2021
Cells
Dorsal root ganglia
Enzymes
Papain : 35 u/ml
Stemxyme 1 : 0.2%
Medium
see reference
Neural
Mouse

Mouse, 4-6 week

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Mouse

Mouse

Yousuf, M. , Moreno, M. , Li, J. , He, L. , Royer, D. , Zhang, J. , Woodall, B. , Grace, P. and Price, T.
Diroximel fumarate acts hrough Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons. ,
bioRxivorg
 Version 1 , Preprint Dec 23 , 2023
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, day 2
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Mouse
Mouse
Harris, M. , Hulseberg, P. , Ling, C. , Karman, J. , Clarkson, B. , Harding, J. , Zhang, M. , Sandor, A. , Christensen, K. , Nagy, A. , Sandor, Matyas,S. and Fabry, Z.
Immune Privilege of the CNS is not the Consequence of Limited Antigen Sampling. ,
Sci Rep
 4 , 4422 , 2014
Cells
Oligodendrocytes
Enzymes
Papain : 0.1%
Medium
HBSS
Neural
Mouse
Mouse
Stuart, M. , Corrigan, F. and Baune, B.
Knockout of CXCR5 Increases the Population of Immature Neural Cells and Decreases Proliferation in the Hippocampal Dentate Gyrus. ,
J Neuroinflammation
 11 , 31 , 2014
Cells
Neurosperes
Enzymes
Papain : 0.1%
Medium
NeuroCult NSC
Neural
Mouse
Mouse
Zhang, Y. and Hu, W.
Mouse Enteric Neuronal Cell Culture. ,
Methods Mol Biol
 1078 , 55-63 , 2013
Cells
Enteric neurons
Enzymes
Trypsin : 0.05%
Deoxyribonuclease I : 400 u/ml
Medium
DMEM/F12
Neural
Mouse
Mouse, 6-8 week
Bracko, O. , Singer, T. , Aigner, S. , Knobloch, M. , Winner, B. , Ray, J. , Clemenson, G. , Suh, H. , Couillard-Despres, S. , Aigner, L. , Gage, F. and Jessberger, S.
Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis. ,
J Neurosci
 32 , 3376-87 , 2012
Cells
Neural stem cells
Enzymes
Medium
DMEM/F12
Neural
Mouse
Mouse, P4-5
Ziegler, A. , Schneider, J. , Qin, M. , Tyler, W. , Pintar, J. , Fraidenraich, D. , Wood, T. and Levison, S.
IGF-II Promotes Stemness of Neural Restricted Precursors. ,
Stem Cells
 30 , 1265 , 2012
Cells
Neuroshere
Enzymes
Trypsin : 0.25%
Papain : 100 u
Medium
Pro-N
Neural
Mouse
Mouse, 1-2 day
O'Meara, R. , Ryan, S. , Colognato, H. and Kothary, R.
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues. ,
J Vis Exp
 54 , 3324 , 2011
Cells
Oligodendrocytes, dorsal root ganglia
Enzymes
Papain : 0.15%
Medium
DMEM
Neural
Mouse
Mouse
Babu, H. , Claasen, J. , Kannan, S. , Runker, A. , Palmer, T. and Kempermann, G.
A Protocol for Isolation and Enriched Monolayer Cultivation of Neural Precursor Cells from Mouse Dentate Gyrus. ,
Front Neurosci
 5 , 89 , 2011
Cells
Dentate gyrus neural precursor
Enzymes
Papain : 2.5 u/ml
Neutral Protease : 1.0 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Neurobasal A
Neural
Mouse
Mouse, embryo, 14 day
Pollari, E. , Savchenko, E. , Jaronen, M. , Kanninen, K. , Malm, T. , Wojciechowski, S. , Ahtoniemi, T. , Goldsteins, G. , Giniatullina, R. , Giniatullin, R. , Koistinaho, J. and Magga, J.
Granulocyte Colony Stimulating Factor Attenuates Inflammation in a Mouse Model of Amyotrophic Lateral Sclerosis. ,
J Neuroinflammation
 8 , 74 , 2011
Cells
Spinal cord neurons
Enzymes
Papain : 0.05%
Medium
PBS/DMEM
Neural
Mouse
Mouse
Brown, J. , Gianino, S. and Gutmann, D.
Defective cAMP Generation Underlies the Sensitivity of CNS Neurons to Neurofibromatosis-1 Heterozygosity ,
J Neurosci
 30 , 5579 , 2010
Cells
Hippocampal and retinal neurons
Enzymes
Papain : 1%
Medium
HBSS
Neural
Mouse
Mouse, embryonic
Radad Khaled, Gille Gabriele, Rausch Wolf-Dieter.
Dopaminergic neurons are preferentially sensitive to long-term rotenone toxicity in primary cell culture ,
Toxicol In Vitro
 22 , 68-74 , 2008
Cells
Dopaminergic neurons
Enzymes
Trypsin : 0.1%
Medium
DMEM
Neural
Mouse
Mouse, C57BL
Okano-Uchida T, Himi T, Komiya Y. and Ishizaki Y
Cerebellar granule cell precursors can differentiate into astroglial cells ,
Proc Natl Acad Sci U S A
 101 , 1211 , 2004
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 16.5 u/ml
Medium
Dulbecco's PBS
Neural
Mouse
Mouse, 1-3 day
Savchenko V, Sung U, Blakely RD.
Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody ,
Mol Cell Neurosci
 24 , 1131 , 2003
Cells
Neurons, ganglia
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 100 u/ml
Collagenase : 0.3%
Trypsin : 0.05%
Medium
HBSS
Neural
Mouse
mouse, 65 days
Richards L. , Kilpatrick T. and Bartlett P.
De Novo Generation of Neuronal Cells from the Adult Mouse Brain ,
Proc Natl Acad Sci U S A
 89 , 8591 , 1992
Cells
Neurons, neuronal precursors
Enzymes
Trypsin : 0.1%
Medium
DMEM
Neural
Rat
Rat, SD, p7
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, embryonic
Oyanagi, K. , Tashiro, T. and Negishi, T.
Cell-Type-Specific and Differentiation-Status-Dependent Variations in Cytotoxicity of Tributyltin in Cultured Rat Cerebral Neurons and Astrocytes. ,
J Toxicol Sci
 40 , 459-68 , 2015
Cells
Cerebral neurons
Enzymes
Papain : 2 u/ml
Medium
DMEM-PBS
Neural
Rat
Rat, Fisher, 8-9 week
Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.
The adult substantia nigra contains progenitor cells with neurogenic potential ,
J Neurosci
 22 , 6639 , 2002
Cells
Adult progenitor
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.