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Tissue References Search Tool

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Miscellaneous
Mouse
Mouse, embryonic
He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.
Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
 9 , 53085 , 2020
Cells
Trachea
Enzymes
Elastase : 0.1%
Trypsin : 0.125%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse
Bertoncello, I. and Williams, B.
Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining. ,
Methods Mol Biol
 263 , 181 , 2004
Cells
Bone marrow
Enzymes
Medium
PBS
Miscellaneous
Porcine
Porcine, 6 month
Kean, T. and Dennis, J.
Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions. ,
PLoS ONE
 10 , e0129961 , 2015
Cells
Synoviocytes
Enzymes
Hyaluronidase : 660 u/ml
Trypsin : 0.25%
Collagenase Type 2 : 583 u/ml
Medium
DMEM
Miscellaneous
Rabbit
Rabbit, New Zealand, 8-10 week
Zhang, J. and Wang, J.
Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes. ,
BMC Musculoskelet Disord
 11 , 10 , 2010
Cells
Tenocytes and tendon stem cells
Enzymes
Medium
DMEM
Miscellaneous
Rat
Rat, SD, 6-8 month
Zhou, B. , Zhou, Y. and Tang, K.
The Effects of Substance P on Pluripotent Tendon Cells: an In Vitro and In Vivo Study. ,
J Musculoskelet Neuronal Interact
 14 , 349-58 , 2014
Cells
Pluripotent tendon
Enzymes
Medium
DMEM
Muscle
Canine
Canine
Parker, M. , Loretz, C. , Tyler, A. , Snider, L. , Storb. R. and Tapscott, S.
Inhibition of CD26/DPP-IV Enhances Donor Muscle Cell Engraftment and Stimulates Sustained Donor Cell Proliferation. ,
Skelet Muscle
 2 , 4 , 2012
Cells
Skeletal muscle
Enzymes
Collagenase Type 4 : 200 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM
Muscle
Fish
Zebrafish
Alexander, M. , Kawahara, G. , Kho, A. , Howell, M. , Pusack, T. , Myers, J. , Montanaro, F. , Zon, L. , Guyon, J. and Kunkel, L.
Isolation and Transcriptome Analysis of Adult Zebrafish Cells Enriched for Skeletal Muscle Progenitors. ,
Muscle Nerve
 43 , 741-50 , 2011
Cells
Myogenic progenitor
Enzymes
Medium
PBS
Muscle
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
Muscle
Human
Human, male
Stadler, G. , Chen, J. , Wagner, K. , Robin, J. , Shay, J. , Emerson, C. and Wright, W.
Establishment of Clonal Myogenic Cell Lines from Severely Affected Dystrophic Muscles - CDK4 Maintains the Myogenic Population. ,
Skelet Muscle
 1 , 12 , 2011
Cells
Myogenic
Enzymes
Medium
HBSS
Muscle
Mouse

Mouse

Ghaibour, K. , Rizk, J. , Ebel, C. , Ye, T. , Philipps, M. , Schreiber, V. , Metzger, D. and Duteil, D.
An Efficient Protocol for CUT&RUN Analysis of FACS-Isolated Mouse Satellite Cells. ,
J Vis Exp
 , 2023
Cells
Satellite cells
Enzymes
Medium
DMEM/F-12
Muscle
Mouse
Mouse, 2 month
Abou-Khalil, R. , Yang, F. , Lieu, S. , Julien, A. , Perry, J. , Pereira, C. , Relaix, F. , Miclau, T. , Marcucio, R. and Colnot, C.
Role of Muscle Stem Cells During Skeletal Regeneration. ,
Stem Cells
 33 , 1501-11 , 2015
Cells
Myoblasts
Enzymes
Collagenase Type 2 : 400 u/ml
Neutral Protease : 0.5 u/ml
Medium
Hams F-10
Muscle
Mouse
Mouse, 1-25 month
Ieronimakis Nicholas, Balasundaram Gayathri, Reyes Morayma.
Direct isolation, culture and transplant of mouse skeletal muscle derived endothelial cells with angiogenic potential ,
PLoS ONE
 3 , e0001753 , 2008
Cells
Myocytes, endothelial
Enzymes
Medium
PBS
Muscle
Porcine
Porcine, 2 month
Lewis, F. , Henning, B. , Marazzi, G. , Sassoon, D. , Ellison, G. and Nadal-Ginard, B.
Porcine Skeletal Muscle-Derived Multipotent PW1pos/Pax7neg Interstitial Cells: Isolation, Characterization, and Long-Term Culture. ,
Stem Cells Transl Med
 3 , 702-12 , 2014
Cells
Skeletal muscle
Enzymes
Medium
HBSS
Muscle
Rabbit
Rabbit, chinchilla, 5-8 month, virgin, female, 2-3 kg
Peters, T. , Muller, M. and deDuve, C.
Lysosomes of the Arterial Wall. I. Isolation and Subcellular Fractionation of Cells from Normal Rabbit Aorta ,
J Exp Med
 136 , 1117 , 1972
Cells
Smooth muscle, aorta
Enzymes
Hyaluronidase : 800 u/ml
Medium
HBSS
Muscle
Rat
Rat, day 7
Vilmont, V. , Cadot, B. , Ouanoumou, G. and Gomes, E.
A System for Studying Mechanisms of Neuromuscular Junction Development and Maintence ,
Development
 143 , 2464 , 2016
Cells
Skeletal muscle
Enzymes
Collagenase : 0.05%
Medium
DMEM
Muscle
Rat
Rat
Pacak, C. and Cowan, D.
Fabrication of Myogenic Engineered Tissue Constructs. ,
J Vis Exp
 27 , e1137 , 2009
Cells
Myogenic
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat
Larkin, L. , Calve, S. , Kostrominova, T. and Arruda, E.
Structure and Functional Evaluation of Tendon-Skeletal Muscle Constructs Engineered In Vitro. ,
Tiss Eng
 12 , 3149-58 , 2006
Cells
myogenic
Enzymes
Neutral Protease : 8 u/ml
Collagenase Type 4 : 200 u/ml
Medium
DMEM
Muscle
Rat
Rat, Lewis, neonatal
Kim, J. , Hadlock, T. , Cheney, M. , Varvares, M. and Marler, J.
Muscle Tissue Engineering for Partial Glossectomy Defects. ,
Arch Facial Plast Surg
 5 , 403 , 2003
Cells
Myoblasts
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat, SD, adult and neonatal w/in 1 day of birth
Dennis, R. , Kosnik II, P. , Gilbert, M. and Faulkner, J.
Excitability and Contractility of Skeletal Muscle Engineered from Primary Cultures and Cell Lines ,
Am J Physiol Cell Physiol
 280 , C288 , 2001
Cells
Myooids
Enzymes
Neutral Protease : 4 u/ml
Medium
Ham's F-12
Neural
Avian
Quail and Chicken, embryonic
Rollo, B. , Zhang, D. , Simkin, J. , Menheniott, T. and Newgreen, D.
Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells. ,
F1000Res
 4 , 113 , 2015
Cells
Midgut DRG
Enzymes
Medium
Ham's F12
Neural
Canine
Canine
Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.
Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
 7 , 13915 , 2017
Cells
Satellite glial
Enzymes
Medium
HBSS
Neural
Chicken
Chick, White Leghorn, embryos, 7 day
Wiseman, L. and Hammond, W.
The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
 197 , 429 , 1976
Cells
Neural retina
Enzymes
Collagenase : 0.25%
Elastase : 0.2%
Hyaluronidase : 1.0%
Papain : 1.0%
Protease : 0.1%
Trypsin : 0.05%
Medium
HBSS
Neural
Human

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Human

Human

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.
Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
 188 , 114520 , 2021
Cells
Dorsal root ganglia
Neural
Enzymes
Papain : 30 u/ml
Medium
HBSS
Neural
Human
Human, 9 month
Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.
EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy ,
Nat Med
 11 , 277 , 2005
Cells
Neurons
Enzymes
Papain : 0.07 u/ml
Neutral Protease : 1 mg/ml
Medium
DMEM/F12
Neural
Human
Human, 26 week
Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.
Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells ,
J Neurosci Res
 75 , 782 , 2004
Cells
Neural progenitor cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Neural
Human
Human
Dib-Hajj SD, Tyrrell L, Cummins TR, Black JA, Wood PM, Waxman SG.
Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons ,
F.E.B.S. Lett.
 462 , 117 , 1999
Cells
Dorsal root ganglion neurons
Enzymes
Medium
DMEM/F-12
Neural
Insect
Drosophila
Sanchez-Soriano, N. , Bottenberg, W. , Fiala, A. , Haessler, U. , Kerassoviti, A. , Knust, E. , Lohr, R. and Prokop, A.
Are Dendrites in Drosophila Homologous to Vertebrate Dendrites? ,
Dev Biol
 288 , 126 , 2005
Cells
Dendrites
Enzymes
Collagenase : 0.05%
Medium
HBSS
Neural
Insect
Gryllus Bimaculatus
Kloppenburg, P. and Horner, M.
Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus ,
J Exp Biol
 201 (Pt 17) , 2529 , 1998
Cells
Giant interneurons
Enzymes
Collagenase : 0.05%
Medium
Leibovitz's L15
Neural
Monkey

Rhesus macaque, female

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.
Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
 188 , 114520 , 2021
Cells
Dorsal root ganglia
Neural
Enzymes
Papain : 30 u/ml
Medium
HBSS
Neural
Mouse

Mouse, 4-6 week

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, adult
Weng, Y. , An, R. , Cassin, J. , Joseph, J. , Mi, R. , Wang, C. , Zhong, C. , Jin, S. , Pfeifer, G. , Bellacosa, A. , Dong, X. , Hoke, A. , He, Z. , Song, H. and Ming, G.
An Intrinsic Epigenetic Barrier for Functional Axon Regeneration ,
Neuron
 94 , 337 , 2018
Cells
Lumbar DRG
Enzymes
Collagenase Type 2 : 200 u/ml
Neutral Protease : 2.5 u/ml
Medium
HBSS
Neural
Mouse
Mouse, embryonic 12.5
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Mouse
Mouse
Xu, W. , Sachewsky, N. , Azimi, A. , Hung, M. , Gappasov, A. and Morshead, C.
Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. ,
Stem Cells
 35 , 485-496 , 2017
Cells
Neurospheres
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.08%
Medium
Neurobasal A
Neural
Mouse
Mouse, 4 month
Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.
Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
 7 , 13915 , 2017
Cells
Satellite glial
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, 2-3 month
Sun, H. , Tsai, W. , Li, B. , Tao, W. , Chen, P. and Rubart, M.
Voltage-Induced Calcium Release in Postganglionic Sympathetic Neurons in Adult Mice. ,
PLoS ONE
 11 , e0148962 , 2016
Cells
Sympathetic neurons
Enzymes
Papain : 10 u/ml
Medium
EBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.