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Tissue References Search Tool

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Adipose/Fat
Human
Human
Esteve, D. , Boulet, N. , Volat, F. , Zakaroff-Girard, A. , Ledoux, S. , Coupaye, M. , Decaunes, P. , Belles, C. , Gaits-Iacovoni, F. , Iacovoni, J. , Remaury, A. , Castel, B. , Ferrara, P. , Heymes, C. and Lafonta, M. Boloumie, J.
Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells. ,
Stem Cells
 33 , 1277-91 , 2015
Cells
Adipose stroma-vascular
Enzymes
Neutral Protease : 2.4 u/ml
Collagenase : 250 u/ml
Medium
PBS
Adipose/Fat
Human
Human
Nie, J. , Chang, B. , Traktuev, D. , Sun, J. , March, K. , Chan, L. , Sage, H. , Pasqualini, R. , Arap, W. and Kolonin, M.
IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells. ,
Stem Cells
 26 , 2735 , 2008
Cells
Stromal
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Adrenal
Mouse
Mouse, 8-10 week
Kolski-Andreaco, A. , Cai, H. , Currle, D. , Chandy, K. and Chow, R.
Mouse Adrenal Chromaffin Cell Isolation. ,
J Vis Exp
 2 , 129 , 2007
Cells
Chromaffin
Enzymes
Papain : 40 u/ml
Medium
DMEM
Adrenal
Mouse
Mouse, embryonic
Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang.
The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells ,
J Neurosci
 25 , 10546-55 , 2005
Cells
Chromafin cells
Enzymes
Papain : 20-25 u/ml
Medium
DMEM
Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Bone
Mouse
Mouse
Lee, D. , Choung, H. , Kim, H. , Gronostajski, R. , Yang, Y. , Ryoo, H. , Lee, Z. , Kim, H. , Cho, E. and Park, J.
NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. ,
Stem Cells
 32 , 2467-79 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Brain
Bovine
Bovine
Kanda, T. , Yoshino, H. , Ariga, T. , Yamawaki, M. and Yu, R.
Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy ,
J Cell Biol
 126 (1) , 235 , 1994
Cells
Microvascular endothelial
Enzymes
Neutral Protease : 0.005%
Medium
Medium 199
Brain
Bovine
Bovine
Miller, D. , Audus, K. and Borchardt, R.
Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier ,
J Tiss Cul Meth
 14 , 217 , 1992
Cells
Endothelial
Enzymes
Neutral Protease : 0.125%
Medium
MEM
Brain
Bovine
Bovine
Audus, K. and Borchardt, R.
Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism ,
Pharm Res
 3 (2) , 81 , 1986
Cells
Endothelial
Enzymes
Medium
MEM
Brain
Human

Human

Nolle, A. , Dijken, I. , Waelti, C. , Calini, D. , Bryois, J. and Lezan, E.
Enrichment of Glial Cells From Human Post-mortem Tissue for Transcriptome and Proteome Analysis Using Immunopanning. ,
Front Cell Neurosci
 15 , 772011 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Human
Human, adult
Spaethling, J. , Na, Y. , Lee, J. , Ulyanova, A. , Baltuch, G. , Bell, T. , Brem, S. , Chen, H. , Dueck, H. , Fisher, S. , Garcia, M. , Khaladkar, M. , Kung, D. , Lucas, T. , O'Rourke, D. and Stefanik, D.
Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics. ,
Cell Rep
 18 , 791-803 , 2017
Cells
Neuronal
Enzymes
Papain : 20 u/ml
Medium
Neurobasal
Brain
Human
Human
Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M.
Isolation of Highly Enriched Primary Human Microglia for Functional Studies. ,
Sci Rep
 6 , 19371 , 2016
Cells
Microglia
Enzymes
Neutral Protease : 10 u/ml
Papain : 2.5 u/ml
Medium
Hibernate A
Brain
Human
Human, fetal and mature
Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B.
Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. ,
Neuron
 89 , 37-53 , 2016
Cells
Astrocytes and neurons
Enzymes
Papain : 7.5-20 u/ml
Medium
RPMI
Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Insect
Drosophilia
Hadzic, T. , Park, D. , Abruzzi, K. , Yang, L. , Trigg, J. , Rohs, R. , Rosbash, M. and Taghert, P.
Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED. ,
Nucleic Acids Res
 43 , 2199-215 , 2015
Cells
Neurons
Enzymes
Papain : 50 u/ml
Medium
Modified Dissecting Saline
Brain
Insect
Drosophila
Gu, H. and O'Dowd, D.
Whole Cell Recordings from Brain of Adult Drosophila. ,
J Vis Exp
 6 , 248 , 2007
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Saline
Brain
Insect
Drosophila
Sicaeros, B. Campusano, J. and O'Dowd, D.
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. ,
J Vis Exp
 4 , 200 , 2007
Cells
Neurons
Enzymes
Papain : 4 u/ml
Medium
DMEM
Brain
Insect
Drosophilia
Gu, H. and O'Dowd, D.
Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ. ,
J Neurosci
 26 , 265-72 , 2006
Cells
Kenyon
Enzymes
Papain : 20 u/ml
Medium
Recording saline
Brain
Mouse
Adult and neonatal
Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P.
Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Cells
Microglia, astrocytes, lymphocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Papain : 20 u/ml
Neutral Protease : 1.2 u/ml
Medium
EBSS
Brain
Mouse
Mouse
Kramer, D. , Risso, D. , Kosillo,. , Ngai, J. and Bateup, H.
Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability , eNeuro Vol. 5 , 1 , 2018
Cells
Neurons
Enzymes
Papain : 10 u/ml
Medium
Artificial CSF
Brain
Mouse
Mouse, adult
Swartzlander, D. , Propson, N. , Roy, E. , Saito, T. , Saido, T. , Wang, B. and Zheng, H.
Concurrent Cell Type-Specific Isolation and Profiling of Mouse Brains in Inflammation and Alzheimer's Disease. ,
JCI Insight
 3 , 2018
Cells
Astrocytes, microglia, endothelia, oligodendrocytes
Enzymes
Papain : 20 u/ml
Medium
HBSS
Brain
Mouse
Mouse, fetal and mature
Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B.
Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. ,
Neuron
 89 , 37-53 , 2016
Cells
Astrocytes and neurons
Enzymes
Papain : 7.5-20 u/ml
Medium
RPMI
Brain
Mouse
Mouse, 3 week
Luo,Y.,Coskun, V. , Liang, A.,Yu, J. , Cheng, L.,Ge, W. , Shi, Z. , Zhang, K. , Li, C. , Lin, H. , Luo, D. , Wang, J. , Lin, C. , Dai, Z. , Zhu, H. , Zhang, J. , Liu, J. , Liu, H.,deVellis, J. , Horvath, S. , Sun, Y. and Li, S.
Different Patterns of Molecular Forms of Somatostatin are Released by the Rat Median Eminence and Hypothalamus. ,
Cell
 161 , 1175 , 2015
Cells
Neural
Enzymes
Papain : 20 u/ml
Medium
PBS
Brain
Mouse
Mouse, P13-15
Haseleu, J. , Anlauf, E. , Blaess, S. , Endl, E. and Derouiche, A.
Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs). ,
Front Cell Neurosci
 7 , 54 , 2013
Cells
Glial
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Mouse
Mouse, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
Brain
Mouse
Mouse, 1-2 day
O'Meara, R. , Ryan, S. , Colognato, H. and Kothary, R.
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues. ,
J Vis Exp
 54 , 3324 , 2011
Cells
Oligodendrocytes, dorsal root ganglia
Enzymes
Papain : 0.15%
Medium
DMEM
Brain
Mouse
Mouse, 6-12 week
Chung, W. , Farley, J. , Swenson, A. , Barnard, J. , Hamilton, G. , Chiposi, R. and Drummond, H.
Extracellular Acidosis Activates ASIC-like Channels in Freshly Isolated Cerebral Artery Smooth Muscle Cells. ,
Am J Physiol Cell Physiol
 298 , C1198 , 2010
Cells
Vascular smooth muscle cells
Enzymes
Papain : 0.05%
Elastase : 0.05%
Medium
PBS
Brain
Mouse
Mouse, neonatal
Fasano, C. , Thibault, D. and Trudeau, L.
Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer , Current Protocols in Neuroscience Vol. 44 , 3.21.1 , 2008
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Neurobasal
Brain
Mouse
Mouse, embryonic and postnatal
Hilgenberg, L. and Smith, M.
Preparation of Dissociated Mouse Cortical Neuron Cultures. ,
J Vis Exp
 10 , 562 , 2007
Cells
Cortical neurons
Enzymes
Papain : 4-10 u/ml
Medium
Neurobasal
Brain
Mouse
Mouse
Oliver, T. , Read, T. , Kessler, J. , Mehmeti, A. , Wells, J. , Huynh, T. , Lin, S. and Wechsler-Reya, R.
Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma. ,
Development
 132 , 2425 , 2005
Cells
Granule cell precursors, pre-neoplastic and tumor cells
Enzymes
Papain : 10 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Neurobasal/B27
Brain
Mouse
Mouse, 3 day
Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H.
Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia ,
J Biol Chem
 277 , 4816 , 2002
Cells
Microglia
Enzymes
Papain : 90 u/ml
Deoxyribonuclease I : 2000 u/ml
Medium
Eagle's MEM
Brain
Mouse
Mouse
Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S. and Ishizaki Y.
A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation ,
J Neurosci
 20 , 5756 , 2000
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 0.435%
Medium
EBSS
Brain
Mouse
Mouse, newborn
Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS.
Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice ,
Learn Mem
 5 , 317-30 , 1998
Cells
Hippocampal cells
Enzymes
Papain : 10 u/ml
Medium
DMKM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.