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Tissue References Search Tool

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Muscle
Mouse
Mouse, 1-25 month
Ieronimakis Nicholas, Balasundaram Gayathri, Reyes Morayma.
Direct isolation, culture and transplant of mouse skeletal muscle derived endothelial cells with angiogenic potential ,
PLoS ONE
 3 , e0001753 , 2008
Cells
Myocytes, endothelial
Enzymes
Medium
PBS
Muscle
Ovine
Sheep, adult and neonatal
Driska S. , Laudadio R. , Wolfson M. and Shaffer T.
A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity ,
J Appl Physiol
 86(1) , 427 , 1999
Cells
Tracheal smooth muscle cells
Enzymes
Papain : 0.2%
Medium
MOPS-PSS
Muscle
Porcine
Porcine, 2 month
Lewis, F. , Henning, B. , Marazzi, G. , Sassoon, D. , Ellison, G. and Nadal-Ginard, B.
Porcine Skeletal Muscle-Derived Multipotent PW1pos/Pax7neg Interstitial Cells: Isolation, Characterization, and Long-Term Culture. ,
Stem Cells Transl Med
 3 , 702-12 , 2014
Cells
Skeletal muscle
Enzymes
Medium
HBSS
Muscle
Rat
Rat, day 7
Vilmont, V. , Cadot, B. , Ouanoumou, G. and Gomes, E.
A System for Studying Mechanisms of Neuromuscular Junction Development and Maintence ,
Development
 143 , 2464 , 2016
Cells
Skeletal muscle
Enzymes
Collagenase : 0.05%
Medium
DMEM
Muscle
Rat
Rat
Pacak, C. and Cowan, D.
Fabrication of Myogenic Engineered Tissue Constructs. ,
J Vis Exp
 27 , e1137 , 2009
Cells
Myogenic
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat
Larkin, L. , Calve, S. , Kostrominova, T. and Arruda, E.
Structure and Functional Evaluation of Tendon-Skeletal Muscle Constructs Engineered In Vitro. ,
Tiss Eng
 12 , 3149-58 , 2006
Cells
myogenic
Enzymes
Neutral Protease : 8 u/ml
Collagenase Type 4 : 200 u/ml
Medium
DMEM
Muscle
Rat
Rat, Lewis, neonatal
Kim, J. , Hadlock, T. , Cheney, M. , Varvares, M. and Marler, J.
Muscle Tissue Engineering for Partial Glossectomy Defects. ,
Arch Facial Plast Surg
 5 , 403 , 2003
Cells
Myoblasts
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat, SD, adult and neonatal w/in 1 day of birth
Dennis, R. , Kosnik II, P. , Gilbert, M. and Faulkner, J.
Excitability and Contractility of Skeletal Muscle Engineered from Primary Cultures and Cell Lines ,
Am J Physiol Cell Physiol
 280 , C288 , 2001
Cells
Myooids
Enzymes
Neutral Protease : 4 u/ml
Medium
Ham's F-12
Muscle
Rat
Rat, SD, 250 g
Jaggar JH.
Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells ,
Am J Physiol Cell Physiol
 281 , C439-48 , 2001
Cells
Arterial smooth muscle
Enzymes
Papain : 0.03%
Collagenase : 0.1%
Medium
see reference
Muscle
Rat
Rat, SHRs and WKY, male, 10 - 14 weeks
Bolzon, B. and Cheung, D.
Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
 14 , 137 , 1989
Cells
Smooth muscle, tail arteries
Enzymes
Papain : 0.1%
Medium
HEPES buffer
(see reference)
Neural
Avian
Quail and Chicken, embryonic
Rollo, B. , Zhang, D. , Simkin, J. , Menheniott, T. and Newgreen, D.
Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells. ,
F1000Res
 4 , 113 , 2015
Cells
Midgut DRG
Enzymes
Medium
Ham's F12
Neural
Chicken
Chicken, White Leghorn, embryos, 17-21 day
Raman, I. and Trussell, L.
The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus ,
Neuron
 9 , 173 , 1992
Cells
Neurons
Enzymes
Papain : 40 u/ml
Medium
HEPES
Neural
Chicken
Chick, White Leghorn, embryos, 7 day
Wiseman, L. and Hammond, W.
The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
 197 , 429 , 1976
Cells
Neural retina
Enzymes
Collagenase : 0.25%
Elastase : 0.2%
Hyaluronidase : 1.0%
Papain : 1.0%
Protease : 0.1%
Trypsin : 0.05%
Medium
HBSS
Neural
Human

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Human

Human

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.
Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
 188 , 114520 , 2021
Cells
Dorsal root ganglia
Neural
Enzymes
Papain : 30 u/ml
Medium
HBSS
Neural
Human
Human
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Human
Human, 9 month
Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.
EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy ,
Nat Med
 11 , 277 , 2005
Cells
Neurons
Enzymes
Papain : 0.07 u/ml
Neutral Protease : 1 mg/ml
Medium
DMEM/F12
Neural
Human
Human, 26 week
Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.
Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells ,
J Neurosci Res
 75 , 782 , 2004
Cells
Neural progenitor cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Neural
Human
Human, adult
Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA.
Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone ,
J Neurosci Res
 59 , 321 , 2000
Cells
Ventricular epithelial
Enzymes
Papain : 11.4 u/ml
Medium
DMEM/F12
Neural
Human
Human
Dib-Hajj SD, Tyrrell L, Cummins TR, Black JA, Wood PM, Waxman SG.
Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons ,
F.E.B.S. Lett.
 462 , 117 , 1999
Cells
Dorsal root ganglion neurons
Enzymes
Medium
DMEM/F-12
Neural
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Neural
Insect
Drosophila
Sanchez-Soriano, N. , Bottenberg, W. , Fiala, A. , Haessler, U. , Kerassoviti, A. , Knust, E. , Lohr, R. and Prokop, A.
Are Dendrites in Drosophila Homologous to Vertebrate Dendrites? ,
Dev Biol
 288 , 126 , 2005
Cells
Dendrites
Enzymes
Collagenase : 0.05%
Medium
HBSS
Neural
Insect
Gryllus Bimaculatus
Kloppenburg, P. and Horner, M.
Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus ,
J Exp Biol
 201 (Pt 17) , 2529 , 1998
Cells
Giant interneurons
Enzymes
Collagenase : 0.05%
Medium
Leibovitz's L15
Neural
Monkey
Macaque
Kupari, J. , Usoskin, D. , Parisien, M. , Lou, D. , Hu, Y. , Fatt, M. , Lonnerberg, P. , Spangberg, M. , Eriksson, B. , Barkas, N. , Kharchenko, P. , Lore, K, Khoury, S. , Diatchenko, L. and Ernfors, P.
Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain. ,
Nat Commun
 12 , 1510 , 2021
Cells
Dorsal root ganglia
Enzymes
Papain : 35 u/ml
Stemxyme 1 : 0.2%
Medium
see reference
Neural
Monkey

Rhesus macaque, female

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.
Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
 188 , 114520 , 2021
Cells
Dorsal root ganglia
Neural
Enzymes
Papain : 30 u/ml
Medium
HBSS
Neural
Mouse

Mouse, 4-6 week

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Mouse

Mouse, adult

Golan, N. and Cafferty, W.
Dissociation of Intact Adult Mouse Cortical Projection Neurons for Single-Cell RNA-seq. ,
Star Protocols
 2 , 100941 , 2021
Cells
Cortical neurons
Enzymes
Papain : 13 u/ml
Medium
Artifical Cerebrospinal Fluid
Neural
Mouse
Mouse, adult
Weng, Y. , An, R. , Cassin, J. , Joseph, J. , Mi, R. , Wang, C. , Zhong, C. , Jin, S. , Pfeifer, G. , Bellacosa, A. , Dong, X. , Hoke, A. , He, Z. , Song, H. and Ming, G.
An Intrinsic Epigenetic Barrier for Functional Axon Regeneration ,
Neuron
 94 , 337 , 2018
Cells
Lumbar DRG
Enzymes
Collagenase Type 2 : 200 u/ml
Neutral Protease : 2.5 u/ml
Medium
HBSS
Neural
Mouse
Mouse, 2-3 month
Sun, H. , Tsai, W. , Li, B. , Tao, W. , Chen, P. and Rubart, M.
Voltage-Induced Calcium Release in Postganglionic Sympathetic Neurons in Adult Mice. ,
PLoS ONE
 11 , e0148962 , 2016
Cells
Sympathetic neurons
Enzymes
Papain : 10 u/ml
Medium
EBSS
Neural
Mouse
Mouse
Beaudet, M. , Yang, Q. , Cadau, S. , Blais, M. , Bellenfant, S. , Gros-Louis, F. and Berthod, F.
High Yield Extraction of Pure Spinal Motor Neurons, Astrocytes and Microglia from Single Embryo and Adult Mouse Spinal Cord. ,
Sci Rep
 5 , 16763 , 2015
Cells
Spinal motor neurons, astrocytes, microglia
Enzymes
Papain : 0.2%
Medium
HBSS
Neural
Mouse
Mouse, adult
Choi, H. , Sun, D. and Jakobs, T.
Isolation of Intact Astrocytes from the Optic Nerve Head of Adult Mice. ,
Exp Eye Res
 137 , 103-10 , 2015
Cells
Astrocytes
Enzymes
Papain : ~20 u/ml
Medium
HBSS
Neural
Mouse
Mouse
Harris, M. , Hulseberg, P. , Ling, C. , Karman, J. , Clarkson, B. , Harding, J. , Zhang, M. , Sandor, A. , Christensen, K. , Nagy, A. , Sandor, Matyas,S. and Fabry, Z.
Immune Privilege of the CNS is not the Consequence of Limited Antigen Sampling. ,
Sci Rep
 4 , 4422 , 2014
Cells
Oligodendrocytes
Enzymes
Papain : 0.1%
Medium
HBSS
Neural
Mouse
Mouse
Stuart, M. , Corrigan, F. and Baune, B.
Knockout of CXCR5 Increases the Population of Immature Neural Cells and Decreases Proliferation in the Hippocampal Dentate Gyrus. ,
J Neuroinflammation
 11 , 31 , 2014
Cells
Neurosperes
Enzymes
Papain : 0.1%
Medium
NeuroCult NSC
Neural
Mouse
Mouse, 2 month
Liu, G. , Rustom, N. , Litteljohn, D. , Bobyn, J. , Rudyk, C. , Anisman, H. and Hayley, S.
Use of Induced Pluripotent Stem Cell Derived Neurons Engineered to Express BDNF for Modulation of Stressor Related Pathology. ,
Front Cell Neurosci
 8 , 316 , 2014
Cells
Fibroblasts
Enzymes
Papain : 0.2%
Medium
Hibernate
Neural
Mouse
Mouse
Medina-Rodriguez, E. , Arenzana, F. , Bribian, A. and de Castro, F.
Protocol to Isolate a Large Amount of Functional Oligodendrocyte Precursor Cells from the Cerebral Cortex of Adult Mice and Humans. ,
PLoS ONE
 8 , e81620 , 2013
Cells
Oligodendrocyte precursor cells
Enzymes
Papain : 0.09%
Medium
DMEM
Neural
Mouse
Mouse, adult
Goetz, J. and Trimarchi, J.
Single-Cell Profiling of Developing and Mature Retinal Neurons. ,
J Vis Exp
 62 , e3824 , 2012
Cells
Retinal neurons
Enzymes
Papain : 10-20 ul
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.