Tissue References Search Tool

Mouse

Mouse, adult, 6-12 week

Shioya, T.

A Simple Technique for Isolating Healthy Heart Cells from Mouse Models. ,
J Physiol Sci
57 , 327-35 , 2007

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 0.1%

Trypsin : 0.006%

Protease XIV : 0.006%

Medium

see reference

Mouse

Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi.

Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms ,
J Physiol
575 , 627-44 , 2006

Cells

Ventricular myocytes and mesenteric arterial SMC

Enzymes

Collagenase Type 2 : 0.06%

Papain : 0.175%

Medium

Krebs-Ringer

Mouse

Mouse, 6-8 week

Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.

Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
12(15) , 1875 , 2003

Cells

Vascular smooth muscle

Enzymes

Papain : 10 u/ml

Elastase : .005%

Collagenase : 0.05%

Deoxyribonuclease I : 1000 u/ml

PDS kit : with modifications

Medium

EBSS

Mouse

Mouse, 2-4 mo

Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.

Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
279 , H429-36 , 2000

Cells

Ventricular myocytes

Enzymes

Collagenase Type 2 : 0.05%

Collagenase Type 4 : 0.05%

Protease XIV : 0.002%

Medium

MEM

Ovine

Voigt, N. , Pearman, C. , Dobrev, D. and Dibb, K.

Methods for Isolating Atrial Cells from Large Mammals and Humans. ,
J Mol Cell Cardiol
86 , 187-98 , 2015

Cells

Atrial myocytes

Enzymes

Collagenase Type 2 : 0.055%

Protease XIV : 0.006%

Medium

Tyrode's solution

Rabbit

Rabbit, New Zealand, 3-3.5 kg

Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.

Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
117 , 2687-2692 , 2020

Cells

Cardiac Myocytes

Enzymes

Trypsin : 0.08%

Protease XIV : 0.005%

Collagenase Type 1 : 0.1-0.2%

Medium

HEPES

Rat

Rat, adult

Xu, X. and Colecraft, H.

Primary Culture of Adult Rat Heart Myocytes. ,
J Vis Exp
28 , 1308 , 2009

Cells

Myocytes

Enzymes

Collagenase Type 2 : 0.05%

Protease XIV : 0.02%

Medium

Media 199

Rat

Rat, SD, 200-250 g

Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi.

Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms ,
J Physiol
575 , 627-44 , 2006

Cells

Ventricular myocytes and mesenteric arterial SMC

Enzymes

Collagenase Type 2 : 0.06%

Papain : 0.175%

Medium

Krebs-Ringer

Rat

Rat, SD, adult female

Puri S, Bansal DD, Uskokovic MR. and MacGregor RR.

Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) ,
Am J Physiol/Endo
278(2) , E293 , 2000

Cells

Heart microvascular cells

Enzymes

CLSPA : 250u/ml

Papain : 5 u/ml

Elastase : 0.8 u/ml

Medium

L-15

Intestine

Mouse

Mouse, neonatal and adult

Joseph, N. , He, S. , Quintana, E. , Kim, Y. , Nunez, G. and Morrison, S.

Enteric Glia are Multipotent in Culture but Primarily Form Glia in the Adult Rodent Gut. ,
J Clin Invest
121 , 3398 , 2011

Cells

Myenteric plexus

Enzymes

Collagenase Type 4 : 0.025-0.1%

Papain : 10 u/ml

Deoxyribonuclease I : 100 u/ml

Medium

HBSS

Kidney

Human

Hemstreet, G. , Enoch, P. and Pretlow, T.

Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification ,
Cancer Res
40 , 1043 , 1980

Cells

Malignant

Stromal

Enzymes

Papain : 0.009%

Medium

Sacks solution

Liver

Porcine

Almalla, A. , Elomaa, L. , Bechtella, L. , Daneshgar, A. , Yavvari, P. and Mahfouz, Z.

Papain-Based Solubilization of Decellularized Extracellular Matrix for the Preparation of Bioactive, Thermosensitive Pregels. , Biomacromolecules Vol. 24 , 5620-5637 , 2023

Cells

Decellularized Extracellular Matrix

Enzymes

Papain : 0.05%

Medium

PBS

Lung

Rat

Rat, male, 200-250g

Bakhramov, A, Evans, M. and Kozlowski, R

Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
83 , 337-47 , 1998

Cells

Pulmonary arterial myocytes

Enzymes

Collagenase : 0.15%

Papain : 0.15%

Elastase : 0.05%

Medium

PBS

Mammary

Mouse

Mouse, 11 week

Mueller, S. , Clark, J. , Myers, P. and Korach, K.

Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
143 , 2357 , 2002

Cells

Epithelial

Enzymes

Collagenase Type 3 : 25 u/ml

Hyaluronidase : 0.1%

Protease XIV : 0.05%

Deoxyribonuclease I : 0.04%

Medium

DMEM/F12

Human

Yoon, S. , Elahi, L. , Pașca, A. , Marton, R. , Gordon, A. , Revah, O. , Miura, Y. , Walczak, E. , Holdgate, G. , Fan, H. , Huguenard, J. , Geschwind, D. and Pașca, S.

Reliability of Human Cortical Organoid Generation. ,
Nat Methods
16 , 75-78 , 2019

Cells

Cortical spheroids

Enzymes

Papain : 40 u/ml

Medium

EBSS

Invertebrate

Octopus vulgaris

Maselli, V. , Xu, F. , Syed, N. , Polese, G. and Cosmo, A.

A Novel Approach to Primary Cell Culture for Octopus vulgaris Neurons. ,
Front Physiol
9 , 220 , 2018

Cells

Neurons

Enzymes

Papain : 0.1%

Collagenase Type 4 : 0.4%

Trypsin : 0.1%

Medium

L15-ASW

Invertebrate

Squid

Raman, M. and Mathew, S.

Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny. ,
J Food Sci Technol
51 , 1509-16 , 2014

Cells

Mantle

Enzymes

Collagenase : see reference

Papain : see reference

Medium

Phosphate buffer

Mouse

Mouse, 8-12 week

Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
, 2020

Cells

Urinary bladder

Enzymes

Collagenase Type 1 : 50 u/ml

Collagenase Type 3 : 40 u/ml

Papain : 45 u/ml

Deoxyribonuclease I : 4 u/ml

Collagenase Type 6 : 20 u/ml

Medium

Tyrode solution

Mouse

Kim, Y. , Wang, S. , Tymanskyj, S. , Ma, L. , Tao, H. and Zhang, L.

Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. ,
Sci Rep
6 , 23799 , 2016

Cells

Cochlear

Enzymes

Papain : 20 u/ml

Trypsin : 0.05%

Medium

DMEM

Fish

Dogfish, Squalus acanthias , 2-6 Kg
(also guinea-pig, rabbit, frog)

Mitra, R. and Morad, M.

A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
249 , H1056 , 1985

Cells

Myocytes, heart and stomach

Enzymes

Protease XIV : 0.028%

Medium

Solution C

(see reference)

Frog

Frog, Rana pipiens (50-100 g)
(also guinea-pig, rabbit, dogfish, Squalus acanthias )

Mitra, R. and Morad, M.

A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
249 , H1056 , 1985

Cells

Myocytes, heart and stomach

Enzymes

Protease XIV : 0.028%

Medium

Solution C

(see reference)

Guinea-Pig

Guinea pig, 2-4 wk old, male, female

Firth, T. , Mawe, G. and Nelson, M.

Pharmacology and Modulation of KATP Channels by Protein Kinase C and Phosphatates in Gallbladder Smooth Muscle ,
Am J Physiol Cell Physiol
278 , C1031 , 2000

Cells

Smooth muscle

Gallbladder

Enzymes

Papain : 0.1%

Medium

Krebs solution

Guinea-Pig

Guinea-pig, adult, 250-350 g

Jennings, L. , Xu, Q. , Firth, T. , Nelson, M. and Mawe, G.

Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle ,
Am J Physiol
277 , G1017 , 1999

Cells

Smooth muscle

Gallbladder

Enzymes

Papain : 0.1%

Medium

NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES

Guinea-Pig

Guinea-pig, 200-400 g
(also rabbit, frog, dogfish)

Mitra, R. and Morad, M.

A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
249 , H1056 , 1985

Cells

Myocytes, heart and stomach

Enzymes

Protease XIV : 0.028%

Medium

Solution C

(see reference)

Human

Malysz, J. , Rovner, E. , Wake, R. and Petkov, G.

Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents. ,
J Vis Exp
, 2020

Cells

Bladder smooth muscle

Enzymes

Collagenase Type 2 : 0.1-0.2%

Papain : 0.1-0.2%

Medium

Digestion solution (see reference)

Ovine

Sheep, adult and neonatal

Driska S. , Laudadio R. , Wolfson M. and Shaffer T.

A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity ,
J Appl Physiol
86(1) , 427 , 1999

Cells

Tracheal smooth muscle cells

Enzymes

Papain : 0.2%

Deoxyribonuclease I : 0.1%

Medium

MOPS-PSS

Rabbit

Rabbit, 0.5-1 Kg
(also guinea-pig, frog, dogfish)

Mitra, R. and Morad, M.

A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
249 , H1056 , 1985

Cells

Myocytes, heart and stomach

Enzymes

Protease XIV : 0.028%

Medium

Solution C

(see reference)

Rat

Rat, SD, 250 g

Jaggar JH.

Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells ,
Am J Physiol Cell Physiol
281 , C439-48 , 2001

Cells

Arterial smooth muscle

Enzymes

Papain : 0.03%

Collagenase : 0.1%

Medium

see reference

Rat

Rat, SHRs and WKY, male, 10 - 14 weeks

Bolzon, B. and Cheung, D.

Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
14 , 137 , 1989

Cells

Smooth muscle, tail arteries

Enzymes

Papain : 0.1%

Medium

HEPES buffer

(see reference)

Chicken

Chicken, White Leghorn, embryos, 17-21 day

Raman, I. and Trussell, L.

The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus ,
Neuron
9 , 173 , 1992

Cells

Neurons

Enzymes

Papain : 40 u/ml

Medium

HEPES

Chicken

Chick, White Leghorn, embryos, 7 day

Wiseman, L. and Hammond, W.

The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
197 , 429 , 1976

Cells

Neural retina

Enzymes

Collagenase : 0.25%

Elastase : 0.2%

Hyaluronidase : 1.0%

Papain : 1.0%

Protease : 0.1%

Trypsin : 0.05%

Medium

HBSS

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.

Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024

Cells

DRG neurons

Enzymes

Papain : 0.1%

Collagenase Type 2 : 0.3%

Neutral Protease : 0.2%

Stemxyme 1 : 0.2%

Deoxyribonuclease I : 0.004%

Medium

HBSS

Human

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.

Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
188 , 114520 , 2021

Cells

Dorsal root ganglia

Neural

Enzymes

Papain : 30 u/ml

Collagenase Type 2 : 0.4%

Neutral Protease : 0.4%

Medium

HBSS

Human

Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.

Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
25 , 1560-70 , 2007

Cells

Neural, various

Enzymes

Papain : 12 u/ml

Collagenase/Dispase : see reference

Trypsin : see reference

Medium

see reference