Tissue References Search Tool

Human

Nolle, A. , Dijken, I. , Waelti, C. , Calini, D. , Bryois, J. and Lezan, E.

Enrichment of Glial Cells From Human Post-mortem Tissue for Transcriptome and Proteome Analysis Using Immunopanning. ,
Front Cell Neurosci
15 , 772011 , 2021

Enzymes

Papain : 20 u/ml

Medium

EBSS

Brain

Human

Human, adult

Spaethling, J. , Na, Y. , Lee, J. , Ulyanova, A. , Baltuch, G. , Bell, T. , Brem, S. , Chen, H. , Dueck, H. , Fisher, S. , Garcia, M. , Khaladkar, M. , Kung, D. , Lucas, T. , O'Rourke, D. and Stefanik, D.

Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics. ,
Cell Rep
18 , 791-803 , 2017

Cells

Neuronal

Enzymes

Papain : 20 u/ml

Medium

Neurobasal

Brain

Human

Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M.

Isolation of Highly Enriched Primary Human Microglia for Functional Studies. ,
Sci Rep
6 , 19371 , 2016

Cells

Microglia

Enzymes

Neutral Protease : 10 u/ml

Papain : 2.5 u/ml

Medium

Hibernate A

Brain

Human

Human, fetal and mature

Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B.

Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. ,
Neuron
89 , 37-53 , 2016

Cells

Astrocytes and neurons

Enzymes

Papain : 7.5-20 u/ml

Medium

RPMI

Eye

Salamander

Clarke, R, Wang, J. and Townes-Anderson, E.

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro. ,
J Vis Exp
19 , 911 , 2008

Cells

Neurons

Enzymes

Papain : 14 u/ml

Medium

Ringers

Eye

Salamander

Salamander, 18-25 cm

Townes-Anderson, E. , MacLeish, P. and Raviola, E.

Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase ,
J Cell Biol
100 , 175 , 1985

Cells

Retina

Enzymes

Papain : 14 u/ml

Medium

Saline

Eye

Salamander

Salamander (A. tigrinum)

Bader, C. , MacLeish, P. and Schwartz, E.

Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina ,
Proc Natl Acad Sci U S A
75 , 3507 , 1978

Cells

Photoreceptors, retina

Enzymes

Papain : 0.05%

Medium

(see reference)

Kidney

Human

Hemstreet, G. , Enoch, P. and Pretlow, T.

Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification ,
Cancer Res
40 , 1043 , 1980

Cells

Malignant

Stromal

Enzymes

Papain : 0.009%

Medium

Sacks solution

Miscellaneous

Human

Yoon, S. , Elahi, L. , Pașca, A. , Marton, R. , Gordon, A. , Revah, O. , Miura, Y. , Walczak, E. , Holdgate, G. , Fan, H. , Huguenard, J. , Geschwind, D. and Pașca, S.

Reliability of Human Cortical Organoid Generation. ,
Nat Methods
16 , 75-78 , 2019

Cells

Cortical spheroids

Enzymes

Papain : 40 u/ml

Medium

EBSS

Muscle

Human

Malysz, J. , Rovner, E. , Wake, R. and Petkov, G.

Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents. ,
J Vis Exp
, 2020

Cells

Bladder smooth muscle

Enzymes

Collagenase Type 2 : 0.1-0.2%

Papain : 0.1-0.2%

Medium

Digestion solution (see reference)

Neural

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.

Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024

Cells

DRG neurons

Enzymes

Papain : 0.1%

Collagenase Type 2 : 0.3%

Neutral Protease : 0.2%

Stemxyme 1 : 0.2%

Deoxyribonuclease I : 0.004%

Medium

HBSS

Neural

Human

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.

Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
188 , 114520 , 2021

Cells

Dorsal root ganglia

Neural

Enzymes

Papain : 30 u/ml

Collagenase Type 2 : 0.4%

Neutral Protease : 0.4%

Medium

HBSS

Neural

Human

Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.

Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
25 , 1560-70 , 2007

Cells

Neural, various

Enzymes

Papain : 12 u/ml

Collagenase/Dispase : see reference

Trypsin : see reference

Medium

see reference

Neural

Human

Human, 9 month

Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.

EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy ,
Nat Med
11 , 277 , 2005

Cells

Neurons

Enzymes

Collagenase Type 4 : 1.33%

Papain : 0.07 u/ml

Neutral Protease : 1 mg/ml

Medium

DMEM/F12

Neural

Human

Human, 26 week

Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.

Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells ,
J Neurosci Res
75 , 782 , 2004

Cells

Neural progenitor cells

Enzymes

Papain : 2.5 u/ml

Deoxyribonuclease I : 250 u/ml

Neutral Protease : 1 u/ml

Medium

DMEM/F-12

Neural

Human

Human, adult

Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA.

Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone ,
J Neurosci Res
59 , 321 , 2000

Cells

Ventricular epithelial

Enzymes

Papain : 11.4 u/ml

Deoxyribonuclease I : 10 u/ml

Medium

DMEM/F12

Neural

Salamander

Salamander, 18-25 cm

Townes-Anderson, E. , MacLeish, P. and Raviola, E.

Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase ,
J Cell Biol
100 , 175 , 1985

Cells

Retina

Enzymes

Papain : 14 u/ml

Medium

Saline

Neural

Salamander

Salamander (A.tigrinum)

Bader, C. , MacLeish, P. and Schwartz, E.

Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina ,
Proc Natl Acad Sci U S A
75 , 3507 , 1978

Cells

Photoreceptors, retina

Enzymes

Papain : 0.05%

Medium

(see reference)

Organoids

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.

Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024

Cells

Cerebral organoids

Enzymes

Papain : 20 u/ml

Deoxyribonuclease I : 0.01%

Medium

DMEM/F12

Organoids

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.

Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
15 , 7027 , 2024

Cells

Brain organoids

Enzymes

Papain : 20 u/ml

Deoxyribonuclease I : 0.005%

Medium

EBSS

Stem

Human

Rigamonti, A. , Repetti, G. , Sun, C. , Price, F. , Reny, D. , Rapino, F. , Weisinger, K. , Benkler, C. , Peterson, Q. , Davidow, L. , Hansson, E. and Rubin, L.

Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System. ,
Stem Cell Reports
6 , 993-1008 , 2016

Cells

Motor neurons

Enzymes

Papain : 20 u/ml

Medium

EBSS

Stem

Human

Welter, J. and Solchaga, L.and Penick, K.

Simplification of Aggregate Culture of Human Mesenchymal Stem Cells as a Chondrogenic Screening Assay ,
Biotechniques
42 , 732 , 2007

Cells