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Neural
Mouse

Mouse, 4-6 week

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Mouse

Mouse, adult

Golan, N. and Cafferty, W.
Dissociation of Intact Adult Mouse Cortical Projection Neurons for Single-Cell RNA-seq. ,
Star Protocols
 2 , 100941 , 2021
Cells
Cortical neurons
Enzymes
Papain : 13 u/ml
Medium
Artifical Cerebrospinal Fluid
Neural
Mouse
Mouse, 2-3 month
Sun, H. , Tsai, W. , Li, B. , Tao, W. , Chen, P. and Rubart, M.
Voltage-Induced Calcium Release in Postganglionic Sympathetic Neurons in Adult Mice. ,
PLoS ONE
 11 , e0148962 , 2016
Cells
Sympathetic neurons
Enzymes
Papain : 10 u/ml
Medium
EBSS
Neural
Mouse
Mouse
Beaudet, M. , Yang, Q. , Cadau, S. , Blais, M. , Bellenfant, S. , Gros-Louis, F. and Berthod, F.
High Yield Extraction of Pure Spinal Motor Neurons, Astrocytes and Microglia from Single Embryo and Adult Mouse Spinal Cord. ,
Sci Rep
 5 , 16763 , 2015
Cells
Spinal motor neurons, astrocytes, microglia
Enzymes
Papain : 0.2%
Medium
HBSS
Neural
Mouse
Mouse, adult
Choi, H. , Sun, D. and Jakobs, T.
Isolation of Intact Astrocytes from the Optic Nerve Head of Adult Mice. ,
Exp Eye Res
 137 , 103-10 , 2015
Cells
Astrocytes
Enzymes
Papain : ~20 u/ml
Medium
HBSS
Neural
Mouse
Mouse
Harris, M. , Hulseberg, P. , Ling, C. , Karman, J. , Clarkson, B. , Harding, J. , Zhang, M. , Sandor, A. , Christensen, K. , Nagy, A. , Sandor, Matyas,S. and Fabry, Z.
Immune Privilege of the CNS is not the Consequence of Limited Antigen Sampling. ,
Sci Rep
 4 , 4422 , 2014
Cells
Oligodendrocytes
Enzymes
Papain : 0.1%
Medium
HBSS
Neural
Mouse
Mouse
Stuart, M. , Corrigan, F. and Baune, B.
Knockout of CXCR5 Increases the Population of Immature Neural Cells and Decreases Proliferation in the Hippocampal Dentate Gyrus. ,
J Neuroinflammation
 11 , 31 , 2014
Cells
Neurosperes
Enzymes
Papain : 0.1%
Medium
NeuroCult NSC
Neural
Mouse
Mouse, 2 month
Liu, G. , Rustom, N. , Litteljohn, D. , Bobyn, J. , Rudyk, C. , Anisman, H. and Hayley, S.
Use of Induced Pluripotent Stem Cell Derived Neurons Engineered to Express BDNF for Modulation of Stressor Related Pathology. ,
Front Cell Neurosci
 8 , 316 , 2014
Cells
Fibroblasts
Enzymes
Papain : 0.2%
Medium
Hibernate
Neural
Mouse
Mouse
Medina-Rodriguez, E. , Arenzana, F. , Bribian, A. and de Castro, F.
Protocol to Isolate a Large Amount of Functional Oligodendrocyte Precursor Cells from the Cerebral Cortex of Adult Mice and Humans. ,
PLoS ONE
 8 , e81620 , 2013
Cells
Oligodendrocyte precursor cells
Enzymes
Papain : 0.09%
Medium
DMEM
Neural
Mouse
Mouse, adult
Goetz, J. and Trimarchi, J.
Single-Cell Profiling of Developing and Mature Retinal Neurons. ,
J Vis Exp
 62 , e3824 , 2012
Cells
Retinal neurons
Enzymes
Papain : 10-20 ul
Medium
HBSS
Neural
Mouse
Mouse
Li, Y. , Roy, B. , Wang, W. , Zhang, L. , Sampson, S. and Lin, D.
Imaging pHluorin-Tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons. ,
J Vis Exp
 69 , e4450 , 2012
Cells
Neurons
Enzymes
Papain : 100 ul
Medium
DMEM
Neural
Mouse
Mouse, 6-8 week
Bracko, O. , Singer, T. , Aigner, S. , Knobloch, M. , Winner, B. , Ray, J. , Clemenson, G. , Suh, H. , Couillard-Despres, S. , Aigner, L. , Gage, F. and Jessberger, S.
Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis. ,
J Neurosci
 32 , 3376-87 , 2012
Cells
Neural stem cells
Enzymes
Medium
DMEM/F12
Neural
Mouse
Mouse, P4-5
Ziegler, A. , Schneider, J. , Qin, M. , Tyler, W. , Pintar, J. , Fraidenraich, D. , Wood, T. and Levison, S.
IGF-II Promotes Stemness of Neural Restricted Precursors. ,
Stem Cells
 30 , 1265 , 2012
Cells
Neuroshere
Enzymes
Trypsin : 0.25%
Papain : 100 u
Medium
Pro-N
Neural
Mouse
Mouse, 1-2 day
O'Meara, R. , Ryan, S. , Colognato, H. and Kothary, R.
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues. ,
J Vis Exp
 54 , 3324 , 2011
Cells
Oligodendrocytes, dorsal root ganglia
Enzymes
Papain : 0.15%
Medium
DMEM
Neural
Mouse
Mouse
Babu, H. , Claasen, J. , Kannan, S. , Runker, A. , Palmer, T. and Kempermann, G.
A Protocol for Isolation and Enriched Monolayer Cultivation of Neural Precursor Cells from Mouse Dentate Gyrus. ,
Front Neurosci
 5 , 89 , 2011
Cells
Dentate gyrus neural precursor
Enzymes
Papain : 2.5 u/ml
Neutral Protease : 1.0 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Neurobasal A
Neural
Mouse
Mouse, embryo, 14 day
Pollari, E. , Savchenko, E. , Jaronen, M. , Kanninen, K. , Malm, T. , Wojciechowski, S. , Ahtoniemi, T. , Goldsteins, G. , Giniatullina, R. , Giniatullin, R. , Koistinaho, J. and Magga, J.
Granulocyte Colony Stimulating Factor Attenuates Inflammation in a Mouse Model of Amyotrophic Lateral Sclerosis. ,
J Neuroinflammation
 8 , 74 , 2011
Cells
Spinal cord neurons
Enzymes
Papain : 0.05%
Medium
PBS/DMEM
Neural
Mouse
Mouse, 4-8 week
Radtke, C. , Sasaki, M. , Lankford, K. , Gallo, V. and Kocsis, J.
CNPase Expression in Olfactory Ensheathing Cells. ,
J Biomed Biotechnol
 2011 , 608496 , 2011
Cells
Olfactory ensheathing
Enzymes
Collagenase : 0.15%
Papain : 12 u/ml
Medium
DMEM
Neural
Mouse
Mouse, 30 day
Milligan, C. and Gifondorwa, D.
Isolation and Culture of Postnatal Spinal Motoneurons. ,
Methods Mol Biol
 793 , 77 , 2011
Cells
Motoneurons
Enzymes
Papain : 0.2%
Medium
Hibernate A
Neural
Mouse
Mouse
Brown, J. , Gianino, S. and Gutmann, D.
Defective cAMP Generation Underlies the Sensitivity of CNS Neurons to Neurofibromatosis-1 Heterozygosity ,
J Neurosci
 30 , 5579 , 2010
Cells
Hippocampal and retinal neurons
Enzymes
Papain : 1%
Medium
HBSS
Neural
Mouse
Mouse, adult
Yip, P. , Kaan, T. , Fenesan, D. and Malcangio, M.
Rapid Isolation and Culture of Primary Microglia from Adult Mouse Spinal Cord. ,
J Neurosci Methods
 183 , 223-37 , 2009
Cells
Spinal microganglia
Enzymes
Papain : 0.2%
Medium
Hibernate A
Neural
Mouse
Mouse, 6 mo
Brewer Gregory J, Torricelli John R.
Isolation and culture of adult neurons and neurospheres ,
Nat Protoc
 2 , 1490-8 , 2007
Cells
Neurons, neurospheres
Enzymes
Papain : 0.2%
Medium
Hibernate
Neural
Mouse
Mouse, 6-8 week
Malin, S. , Davis, B. and Molliver, D.
Production of Dissociated Sensory Neuron Cultures and Considerations for their use in Studying Neuronal Function and Plasticity ,
Nat Protoc
 2 , 152 , 2007
Cells
Sensory neurons, DRG
Enzymes
Papain : 20 u/ml
Medium
HBSS
Neural
Mouse
Mouse
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Mouse
Mouse, C57BL/6, 1 year
Eide, L. and McMurray, C
Culture of Adult Mouse Neurons ,
Biotechniques
 38(1) , 99-104 , 2005
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
DMEM
Neural
Mouse
Mouse, C57BL
Okano-Uchida T, Himi T, Komiya Y. and Ishizaki Y
Cerebellar granule cell precursors can differentiate into astroglial cells ,
Proc Natl Acad Sci U S A
 101 , 1211 , 2004
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 16.5 u/ml
Medium
Dulbecco's PBS
Neural
Mouse
Mouse, 1-3 day
Savchenko V, Sung U, Blakely RD.
Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody ,
Mol Cell Neurosci
 24 , 1131 , 2003
Cells
Neurons, ganglia
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 100 u/ml
Collagenase : 0.3%
Trypsin : 0.05%
Medium
HBSS
Neural
Mouse
Mouse, male
Roberts, L. and MacDonald, C.and Mark, C.
Anandamide is a Partial Agonist at Native Vanilloid Receptors in Acutely Isolated Mouse Trigeminal Sensory Neurons. ,
Br J Pharmacol
 137 , 421 , 2002
Cells
Trigeminal senesory neurons
Enzymes
Papain : 20 u/ml
Medium
HEPES buffered saline

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.