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Tissue References Search Tool

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Neural
Chicken
Chick, White Leghorn, embryos, 7 day
Wiseman, L. and Hammond, W.
The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
 197 , 429 , 1976
Cells
Neural retina
Enzymes
Collagenase : 0.25%
Elastase : 0.2%
Hyaluronidase : 1.0%
Papain : 1.0%
Protease : 0.1%
Trypsin : 0.05%
Medium
HBSS
Neural
Human

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Monkey
Macaque
Kupari, J. , Usoskin, D. , Parisien, M. , Lou, D. , Hu, Y. , Fatt, M. , Lonnerberg, P. , Spangberg, M. , Eriksson, B. , Barkas, N. , Kharchenko, P. , Lore, K, Khoury, S. , Diatchenko, L. and Ernfors, P.
Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain. ,
Nat Commun
 12 , 1510 , 2021
Cells
Dorsal root ganglia
Enzymes
Papain : 35 u/ml
Stemxyme 1 : 0.2%
Medium
see reference
Neural
Mouse

Mouse, 4-6 week

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Mouse

Mouse

Yousuf, M. , Moreno, M. , Li, J. , He, L. , Royer, D. , Zhang, J. , Woodall, B. , Grace, P. and Price, T.
Diroximel fumarate acts hrough Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons. ,
bioRxivorg
 Version 1 , Preprint Dec 23 , 2023
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Mouse
Mouse
Yousuf, M. , Samtleben, S. , Lamothe, S. , Friedman, T. , Catuneanu, A. and et al.
ER Stress in the Dorsal Root Ganglia Regulates BK Channel Physiology and Contributes to Pain Hypersensitivity in a Mouse Model of Multiple Schlerosis ,
bioRxivorg
 , 2020
Cells
Dorsal root ganglion
Enzymes
Stemxyme 1 : 0.1-0.2%
Medium
HBSS
Neural
Rat
Rat, adult
George, D. , Ahrens, P. and Lambert, S.
Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells. ,
Glia
 , 2018
Cells
Schwann
Enzymes
Stemxyme 1 : 0.05%
Medium
DMEM
Neural
Shellfish
Mollusc, juvenile 1-2 gm or adult 50-100 gm
Schacher, S. and Proshanksy, E.
Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment ,
J Neurosci
 3 (12) , 2403 , 1983
Cells
Neurons
LUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Neural
Shellfish
Aplysia californica
Camardo, J. , Proshansky, E. and Schacher, S.
Identified Aplysia Neurons Form Specific Chemical Synapses in Culture ,
J Neurosci
 3 (12) , 2614 , 1983
Cells
LUQ cells
RUQ cells
Enzymes
Protease : 1%
Medium
L15 medium

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.