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Tissue References Search Tool

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Adipose/Fat
Mouse

Mouse, Adult

Hay, A. , Murphy, T. , Popichak, K. , Zabel, M. and Moreno, J.
Adipose-derived mesenchymal stromal cells decrease prion-induced glial inflammation in vitro. ,
Sci Rep
 12 , 22567 , 2022
Cells
Mesenchymal stromal
Enzymes
Stemxyme 1 : 400u/ml
Medium
DMEM/F12
Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Cartilage
Rabbit
Rabbit, New Zealand, white, 4-6 wk & 22-25 wk
Plaas, A. , Sandy, J. and Kimura, J.
Biosynthesis of Cartilage Proteoglycan & Link Protein Articular Chondrocytes ,
J Biol Chem
 263 , 7560 , 1988
Cells
Chondrocytes
Enzymes
Protease XIV : 5 mg/g of tissue
Medium
Ham's F-12
Endothelial
Mouse
Mouse, 2 month
Sabbagh, M. and Nathans, J.
A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture ,
Elife
 , 1 , 2020
Cells
Endothelial, CNS
Enzymes
PDS kit : with modifications
Stemxyme 1 : 0.1%
Medium
EC growth medium
Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Heart
Canine
Canine
Harleton, E. , Besana, A. , Comas, G. , Danilo, P. , Rosen, T. , Argenziano, M. , Rosen, M. , Robinson, R. and Feinmark, S.
Ability to Induce Atrial Fibrillation in the Peri-Operative Period is Associated with Phosphorylation-Dependent Inhibition of TWIK Protein-Related Acid-Sensitive Potassium Channel 1 (TASK-1). ,
J Biol Chem
 288 , 2829-38 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 60 u/ml
Protease XIV : 0.5 u/ml
Medium
See reference
Heart
Fish
Zebrafish
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Human
Human
Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N.
Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 283 , H990-5 , 2002
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.013%
Medium
HEPES solution
Heart
Mouse
Mouse, male, 12-18 week
Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R. and Chen-Izu, Y
In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. ,
PLoS ONE
 11 , e0160605 , 2016
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease XIV : 0.004%
Medium
Perfusion solution (see reference)
Heart
Mouse

Mouse, 8-12 week

Ackers-Johnson, M. , Li, P. , Holmes, A. , O'Brien, S. , Pavlovic, D. and Foo, R.
A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. ,
Circ Res
 119 , 909-20 , 2016
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.005%
Medium
Perfusion buffer (see reference)
Heart
Mouse
Mouse, adult, 9-13 week
Kabaeva, Z. , Zhao, M. and Michele, D.
Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. ,
Am J Physiol Heart Circ Physiol
 294 , H1667-74 , 2008
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 620 u/ml
Protease XIV : 0.104 u/ml
Medium
Myocyte buffer see reference
Heart
Mouse
Mouse, adult, 6-12 week
Shioya, T.
A Simple Technique for Isolating Healthy Heart Cells from Mouse Models. ,
J Physiol Sci
 57 , 327-35 , 2007
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.006%
Protease XIV : 0.006%
Medium
see reference
Heart
Mouse
Mouse, 2-4 mo
Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.
Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
 279 , H429-36 , 2000
Cells
Ventricular myocytes
Enzymes
Protease XIV : 0.002%
Medium
MEM
Heart
Ovine
Ovine
Voigt, N. , Pearman, C. , Dobrev, D. and Dibb, K.
Methods for Isolating Atrial Cells from Large Mammals and Humans. ,
J Mol Cell Cardiol
 86 , 187-98 , 2015
Cells
Atrial myocytes
Enzymes
Protease XIV : 0.006%
Medium
Tyrode's solution
Heart
Rabbit
Rabbit, New Zealand, 3-3.5 kg
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Rat
Rat, adult
Xu, X. and Colecraft, H.
Primary Culture of Adult Rat Heart Myocytes. ,
J Vis Exp
 28 , 1308 , 2009
Cells
Myocytes
Enzymes
Protease XIV : 0.02%
Medium
Media 199
Mammary
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse
Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Muscle
Fish
Dogfish, Squalus acanthias , 2-6 Kg
(also guinea-pig, rabbit, frog)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Frog
Frog, Rana pipiens (50-100 g)
(also guinea-pig, rabbit, dogfish, Squalus acanthias )
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Guinea-Pig
Guinea-pig, 200-400 g
(also rabbit, frog, dogfish)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Rabbit
Rabbit, 0.5-1 Kg
(also guinea-pig, frog, dogfish)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Neural
Human

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Monkey
Macaque
Kupari, J. , Usoskin, D. , Parisien, M. , Lou, D. , Hu, Y. , Fatt, M. , Lonnerberg, P. , Spangberg, M. , Eriksson, B. , Barkas, N. , Kharchenko, P. , Lore, K, Khoury, S. , Diatchenko, L. and Ernfors, P.
Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain. ,
Nat Commun
 12 , 1510 , 2021
Cells
Dorsal root ganglia
Enzymes
Papain : 35 u/ml
Stemxyme 1 : 0.2%
Medium
see reference
Neural
Mouse

Mouse, 4-6 week

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Mouse

Mouse

Yousuf, M. , Moreno, M. , Li, J. , He, L. , Royer, D. , Zhang, J. , Woodall, B. , Grace, P. and Price, T.
Diroximel fumarate acts hrough Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons. ,
bioRxivorg
 Version 1 , Preprint Dec 23 , 2023
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Mouse
Mouse
Yousuf, M. , Samtleben, S. , Lamothe, S. , Friedman, T. , Catuneanu, A. and et al.
ER Stress in the Dorsal Root Ganglia Regulates BK Channel Physiology and Contributes to Pain Hypersensitivity in a Mouse Model of Multiple Schlerosis ,
bioRxivorg
 , 2020
Cells
Dorsal root ganglion
Enzymes
Stemxyme 1 : 0.1-0.2%
Medium
HBSS
Neural
Rat
Rat, adult
George, D. , Ahrens, P. and Lambert, S.
Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells. ,
Glia
 , 2018
Cells
Schwann
Enzymes
Stemxyme 1 : 0.05%
Medium
DMEM
Organoids
Mouse

Mouse

Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Stem
Mouse
Mouse, xenograft
Pham, K. , Delitto, D. , Knowlton, A. , Hartlage, E. , Madhavan, R. , Gonzalo, D. , Thomas, R. , Behrns, K. , George, T. , Hughes, S. , Wallet, S. , Liu, C. and Trevino, J.
Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. ,
Am J Pathol
 186 , 2016
Cells
Pancreatic cancer
Enzymes
Stemxyme 1 : 0.2%
Medium
HBSS
Tumor
Human
Human
Pham, K. , Delitto, D. , Knowlton, A. , Hartlage, E. , Madhavan, R. , Gonzalo, D. , Thomas, R. , Behrns, K. , George, T. , Hughes, S. , Wallet, S. , Liu, C. and Trevino, J.
Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. ,
Am J Pathol
 186 , 2016
Cells
Pancreatic cancer
Enzymes
Stemxyme 1 : 0.2%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.