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Tissue References Search Tool

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Adipose/Fat
Mouse

Mouse, Adult

Hay, A. , Murphy, T. , Popichak, K. , Zabel, M. and Moreno, J.
Adipose-derived mesenchymal stromal cells decrease prion-induced glial inflammation in vitro. ,
Sci Rep
 12 , 22567 , 2022
Cells
Mesenchymal stromal
Enzymes
Stemxyme 1 : 400u/ml
Medium
DMEM/F12
Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Brain
Mouse
Mouse, 0-30 day
Shrier, B. , Wilson, S. and Nirenberg, M.
Cultured Cell Systems and Methods for Neurobiology , Vol. 32 , 765 , 1974
Cells
Neural
Enzymes
Medium
BSS
Endothelial
Human
Human
Lewis, L.J. , Haok, J.C. , Maca, R.D. and Fry, G.L.
Replication of Human Endothelial Cells in Culture ,
Science
 181 , 452 , 1973
Cells
Umbilical cord
Enzymes
Trypsin NF 1:250 : 0.125%
Medium
Saline, normal
Endothelial
Human
Human
Fryer, D.G. , Birnbaum, G. and Luttrell, C.N.
Human Endothelium in Cell Culture ,
J Atheroscler Res
 6 , 151 , 1966
Cells
Umbilical cord
Enzymes
Medium
CMF solution
Endothelial
Mouse
Mouse, 2 month
Sabbagh, M. and Nathans, J.
A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture ,
Elife
 , 1 , 2020
Cells
Endothelial, CNS
Enzymes
PDS kit : with modifications
Stemxyme 1 : 0.1%
Medium
EC growth medium
Heart
Chicken
Chick, 8 day embryo
(also rat, neonate and mouse)
Gross, W. , Schopf-Ebner, E. and Bucher, O.
Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells ,
Exp Cell Res
 53 , 1 , 1968
Cells
Myocytes
Enzymes
Medium
Rinaldini's buffer solution, CF
Heart
Mouse
Mouse
(also rat, neonate and chick, 8 day embryo)
Gross, W. , Schopf-Ebner, E. and Bucher, O.
Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells ,
Exp Cell Res
 53 , 1 , 1968
Cells
Myocytes
Enzymes
Medium
Rinaldini's buffer solution, CF
Mammary
Guinea-Pig
Guinea-pig, pregnant, 4-10 day
Turba, F. and Hilpert, N.
Secretion and Resorption of Proteins by Isolated Mammary Gland Cells. German ,
Biochem Z
 334 , 501 , 1961
Cells
Mammary gland
Enzymes
Medium
Dulbecco phosphate
Mammary
Mouse
Mouse, lactating, 14 day
Kopelovich, L. , Abraham, S. , McGrath, H. , DeOme, K. and Chaikoff, I.
Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland ,
Cancer Res
 26 , 1534 , 1966
Cells
Mammary
Enzymes
Medium
HBSS
Miscellaneous
Mouse
Mouse
Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Muscle
Rat
Rat, 3-4 day
Kasten, F.
Rat Myocardial Cells In Vitro: Mitosis and Differentiated Properties ,
In Vitro
 8 , 128 , 1972
Cells
Myocardial
Enzymes
Trypsin NF 1:250 : 0.125%
Medium
HBSS CMF
Muscle
Rat
Rat, Wistar, 3-10 day
Harary, I. and Farley, B.
In Vitro Studies on Single Beating Rat Heart Cells ,
Exp Cell Res
 29 , 451 , 1963
Cells
Heart
Enzymes
Trypsin NF 1:250 : 250: 0.1%
Medium
Saline A
(See reference)
Neural
Human

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Monkey
Macaque
Kupari, J. , Usoskin, D. , Parisien, M. , Lou, D. , Hu, Y. , Fatt, M. , Lonnerberg, P. , Spangberg, M. , Eriksson, B. , Barkas, N. , Kharchenko, P. , Lore, K, Khoury, S. , Diatchenko, L. and Ernfors, P.
Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain. ,
Nat Commun
 12 , 1510 , 2021
Cells
Dorsal root ganglia
Enzymes
Papain : 35 u/ml
Stemxyme 1 : 0.2%
Medium
see reference
Neural
Mouse

Mouse, 4-6 week

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Mouse

Mouse

Yousuf, M. , Moreno, M. , Li, J. , He, L. , Royer, D. , Zhang, J. , Woodall, B. , Grace, P. and Price, T.
Diroximel fumarate acts hrough Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons. ,
bioRxivorg
 Version 1 , Preprint Dec 23 , 2023
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Mouse
Mouse
Yousuf, M. , Samtleben, S. , Lamothe, S. , Friedman, T. , Catuneanu, A. and et al.
ER Stress in the Dorsal Root Ganglia Regulates BK Channel Physiology and Contributes to Pain Hypersensitivity in a Mouse Model of Multiple Schlerosis ,
bioRxivorg
 , 2020
Cells
Dorsal root ganglion
Enzymes
Stemxyme 1 : 0.1-0.2%
Medium
HBSS
Neural
Mouse
Mouse
Salisbury, E. , Lazard, Z. , Ubogu, E. , Davis, A. and Olmsted-Davis, E.
Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2. ,
Stem Cells Transl Med
 1 , 874-85 , 2012
Cells
Nerve progenitors
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, 0-30 day
Shrier, B. , Wilson, S. and Nirenberg, M.
Cultured Cell Systems and Methods for Neurobiology , Vol. 32 , 765 , 1974
Cells
Neural
Enzymes
Medium
BSS
Neural
Rat
Rat, adult
George, D. , Ahrens, P. and Lambert, S.
Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells. ,
Glia
 , 2018
Cells
Schwann
Enzymes
Stemxyme 1 : 0.05%
Medium
DMEM
Organoids
Mouse

Mouse

Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Stem
Human
Human
Lee, S. , Park, B. , Kim, J. , Jekarl, D. , Choi, H. , Lee, S. , Kim, M. , Kim, Y. and Park, M.
The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells. ,
Cytotherapy
 17 , 1723-31 , 2015
Cells
Wharton jelly mesenchymal stem
Enzymes
Medium
PBS
Stem
Mouse
Mouse, xenograft
Pham, K. , Delitto, D. , Knowlton, A. , Hartlage, E. , Madhavan, R. , Gonzalo, D. , Thomas, R. , Behrns, K. , George, T. , Hughes, S. , Wallet, S. , Liu, C. and Trevino, J.
Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. ,
Am J Pathol
 186 , 2016
Cells
Pancreatic cancer
Enzymes
Stemxyme 1 : 0.2%
Medium
HBSS
Stem
Mouse
Mouse
Salisbury, E. , Lazard, Z. , Ubogu, E. , Davis, A. and Olmsted-Davis, E.
Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2. ,
Stem Cells Transl Med
 1 , 874-85 , 2012
Cells
Nerve progenitors
Enzymes
Medium
HBSS
Tumor
Human
Human
Pham, K. , Delitto, D. , Knowlton, A. , Hartlage, E. , Madhavan, R. , Gonzalo, D. , Thomas, R. , Behrns, K. , George, T. , Hughes, S. , Wallet, S. , Liu, C. and Trevino, J.
Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. ,
Am J Pathol
 186 , 2016
Cells
Pancreatic cancer
Enzymes
Stemxyme 1 : 0.2%
Medium
HBSS
Tumor
Mouse
Mouse, lactating, 14 day
Kopelovich, L. , Abraham, S. , McGrath, H. , DeOme, K. and Chaikoff, I.
Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland ,
Cancer Res
 26 , 1534 , 1966
Cells
Mammary
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.