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Adipose/Fat
Ovine

Ovine

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Brain
Monkey
Monkey, rhesus
Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS.
Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection ,
J Immunol
 167 , 5429-38 , 2001
Cells
Brain cells
Enzymes
Medium
HBSS
Brain
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Cartilage
Ovine
Sheep, 2 month
Kojima Koji, Bonassar Lawrence J, Roy Amit K, Mizuno Hirokazu, Cortiella Joaquin, Vacanti Charles A.
A Composite Tissue-Engineered Trachea Using Sheep Nasal Chondrocyte and Epithelial Cells ,
FASEB J
 17 , 823-8 , 2003
Cells
Chondrocytes
Enzymes
Medium
Ham's F-12
Eye
Monkey
Macaques and baboon
Han ,Y. , Jacoby, R. and Wu, S.
Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells. ,
Brain Res
 875 , 175 , 2000
Cells
Retinal
Enzymes
Papain : 20-40 u/ml
Deoxyribonuclease I : 400 u/ml
Medium
Ames' solution
Eye
Monkey
Monkey, cynomolgus, young adult
Whittum-Hudson JA, Taylor HR.
Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection ,
Infect Immun
 57 , 2977 , 1989
Cells
Conjunctival lymphocytes
Enzymes
Medium
RPMI 1640
Heart
Feline
Cat, mongrel, adult, 1.8-2.8 Kg
Silver, L. , Hemwall, E. , Marino, T. and Houser, S.
Isolation and Morphology of Calcium-Tolerant Feline Ventricular Myocytes ,
Am J Physiol
 245 , H891 , 1983
Cells
Myocytes
Ventricular
Enzymes
Medium
Kreb's Henseleit, CF
Kidney
Monkey
Monkey
Melnick, J. and Wallis, C.
Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells ,
Dev Biol
 37 , 77 , 1976
Cells
Kidney
Enzymes
Trypsin : 0.01% - 0.00001%
Medium
Eagle's MEM
Liver
Monkey
Monkey, Macaca mulatta, 3-5.5 kg
Weber, A. and Groyer-Picard, M.and Dagher, I.
Hepatocyte Transplantation Techniques: Large Animal Models. ,
Methods Mol Biol
 481 , 83 , 2009
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 129 u/ml
Medium
DMEM/F12
Liver
Monkey
Monkey (Macaca fascicularis), adult, 5-6 Kg
Ulrich, R. , Aspar, D. , Cramer, C. , Kletzien, R. and Ginsberg, L.
Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis) ,
In Vitro Cell Dev Biol
 26 , 815 , 1990
Cells
Hepatocytes
Enzymes
Trypsin : 160 u/ml
Medium
HEPES buffer
Muscle
Feline
Cat, adult mongrel, either sex, 2.5-4.0 kg
Madden, J. , Vadula, M. and Kurup, V.
Effects of Hypoxia and Other Vasoactive Agents on Pulmonary and Cerebral Artery Smooth Muscle Cells ,
Am J Physiol
 263 , L384 , 1992
Cells
Cerebral arteries
Enzymes
Elastase : 50 u/ml
Medium
Puck's solution
Muscle
Feline
Cat, mongrel, adult, 2-4 kg
Follmer, C.H. , Ten Eick, R.E. and Yeh, J.Z.
Sodium Current Kinetics in Cat Atrial Myocytes ,
J Physiol
 384 , 169 , 1987
Cells
Myocytes
Enzymes
Collagenase : 0.12%
Medium
Kreb's Henseleit, CF
Muscle
Monkey
Monkey (Macaca nemestrina)
Chait, A. , Ross, R. , Albers, J. and Bierman, E.
Platelet-Derived Growth Factor Stimulates Activity of LDL Receptors ,
Proc Natl Acad Sci U S A
 77 , 4084 , 1980
Cells
Smooth muscle
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt
Muscle
Monkey
Rhesus monkey, 1 year
(also human, rabbits)
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture ,
Cell Tissue Res
 177 , 503 , 1977
Cells
Smooth muscle, saphenous vein
Enzymes
Elastase : 0.05%
Medium
BSS
Muscle
Ovine
Sheep, adult and neonatal
Driska S. , Laudadio R. , Wolfson M. and Shaffer T.
A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity ,
J Appl Physiol
 86(1) , 427 , 1999
Cells
Tracheal smooth muscle cells
Enzymes
Papain : 0.2%
Medium
MOPS-PSS
Neural
Monkey
Macaque
Kupari, J. , Usoskin, D. , Parisien, M. , Lou, D. , Hu, Y. , Fatt, M. , Lonnerberg, P. , Spangberg, M. , Eriksson, B. , Barkas, N. , Kharchenko, P. , Lore, K, Khoury, S. , Diatchenko, L. and Ernfors, P.
Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain. ,
Nat Commun
 12 , 1510 , 2021
Cells
Dorsal root ganglia
Enzymes
Papain : 35 u/ml
Stemxyme 1 : 0.2%
Medium
see reference
Neural
Monkey

Rhesus macaque, female

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.
Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
 188 , 114520 , 2021
Cells
Dorsal root ganglia
Neural
Enzymes
Papain : 30 u/ml
Medium
HBSS
Neural
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Pancreas
Monkey
Monkey, 3-5 Kg
Scharp, D. , Murphy, J. , Newton, W. , Ballinger, W. and Lacy, P.
Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats ,
Transplant Proc
 7 , 739 , 1975
Cells
Islets
Enzymes
Hyaluronidase : 0.05%
Medium
HBSS
Reproductive
Monkey
Rhesus macaque
Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB. and Stouffer, RL
Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin. ,
Hum Reprod
 26 , 1061-72 , 2011
Cells
Follicle
Enzymes
Medium
HEPES
Reproductive
Ovine
Sheep
Salamonsen, L. , Sum O, W. , Doughton, B. and Findlay, J.
The Effects of Estrogen and Progesterone In Vivo on Protein Synthesis and Secretion by Cultured Epithelial Cells from Sheep Endometrium ,
Endocrinology
 117 (5) , 2148 , 1985
Cells
Epithelial
Enzymes
Collagenase : 125 - 190 u/ml
Medium
DMEM
Stem
Monkey
Monkey
Chen, S. , Revoltella, R. , Papini, S. , Michelini, M. , Fitzgerald, W. and Zimmerberg, J.,and Margolis, L.
Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems ,
Stem Cells
 21(3) , 281 , 2003
Cells
Embryonic stem
Enzymes
Medium
DMEM
Stem
Monkey
Baboons, 1yr, 7yr, 14 yr
Nagano, M. , McCarrey, J. and Brinster, R.
Primate Spermatogonial Stem Cells Colonize Mouse Testes ,
Biol Reprod
 64 , 1409 , 2001
Cells
Primate spermatogonial
Enzymes
Medium
DMEM
Thyroid/Parathyroid
Ovine
Sheep
Kerkof, P.
Preparation of Primary Cultures of Ovine Thyroid Gland Cells ,
J Tiss Cul Meth
 7 , 23 , 1982
Cells
Thyroid
Enzymes
Collagenase : 0.2%
Medium
Puck's Saline F

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.