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Tissue References Search Tool

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Heart
Mouse
Mouse
Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi.
Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms ,
J Physiol
 575 , 627-44 , 2006
Cells
Ventricular myocytes and mesenteric arterial SMC
Enzymes
Papain : 0.175%
Medium
Krebs-Ringer
Heart
Mouse
Mouse, 1 day
Potts, Malia B. , Vaughn, Allyson E. , McDonough, Holly, Patterson, Cam. and Deshmukh, Mohanish
Reduced Apaf-1 levels in cardiomyocytes engage strict regulation of apoptosis by endogenous XIAP ,
J Cell Biol
 171 , 925 , 2005
Cells
Cardiomyoctes
Enzymes
NCIS kit : per instructions
Medium
EBSS
Heart
Mouse
Mouse
Kobayashi, M. , Inoue, K. , Warabi, E. , Minami, T. and Kodama, T.
A Simple Method of Isolating Mouse Aortic Endothelial Cells. ,
J Atheroscler Thromb
 12 , 138 , 2005
Cells
Aortic endothelial
Enzymes
Medium
DMEM
Heart
Mouse
Mouse, neonatal
Takahashi N, Wang X, Tanabe S, Uramoto H, Jishage K, Uchida S, Sasaki S, Okada Y.
ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes ,
Cell Physiol Biochem
 15 , 263 , 2005
Cells
Cardiomyoctes
Enzymes
Collagenase Type 1 : 150 u/ml
Trypsin : 0.01%
Medium
M199
Heart
Mouse
Mouse
Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori.
Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques ,
Circulation
 110 , 1953-9 , 2004
Cells
Smooth muscle cells
Enzymes
Elastase : 0.0125%
Medium
DMEM
Heart
Mouse
Mouse, female, 14-18 g
Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H.
Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo ,
Circulation
 110 , 3803-7 , 2004
Cells
Cardiomyocytes
Enzymes
Elastase : 0.03%
Pancreatin : 0.06%
Medium
see reference
Heart
Mouse
Mouse, 6-8 week
Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.
Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
 12(15) , 1875 , 2003
Cells
Vascular smooth muscle
Enzymes
Papain : 10 u/ml
Elastase : .005%
Collagenase : 0.05%
Deoxyribonuclease I : 1000 u/ml
PDS kit : with modifications
Medium
EBSS
Heart
Mouse
Mouse
Rybkin, I. , Markham, D. , Yan, Z. , Bassel-Duby, R. , Williams, R. and Olson, E.
Conditional Expression of SV40 T-antigen in Mouse Cardiomyocytes Facilitates an Inducible Switch from Proliferation to Differentiation ,
J Biol Chem
 278(18) , 15927 , 2003
Cells
Cardiomyocytes
Enzymes
Medium
DMEM/F-12
Heart
Mouse
Mouse, neonatal 2-4 day
Bettahi, I,, Marker, C. , Roman, M. and Wickman, K.
Contribution of the Kir3.1 Subunit to the Muscarinic-gated Atrial Potassium Channel IKACh ,
J Biol Chem
 277 , 48282 , 2002
Cells
Atrial myocytes
Enzymes
NCIS kit : with modifications see reference
Medium
L-15
Heart
Mouse
Mouse, neonatal and adult
Schreiber, K. , Paquet, L. , Allen, B. and Rindt, H.
Protein Kinase C Isoform Expression and Activity in the Mouse Heart ,
Am J Physiol Heart Circ Physiol
 281(5) , H2062 , 2001
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 0.03-0.2%
Medium
Joklik's MEM
Heart
Mouse
Mouse, 1-6 day
Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G. and Coles JG.
Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture ,
Transplantation
 70 , 1310 , 2000
Cells
Cardiomyocytes
Enzymes
Medium
PBS
Heart
Mouse
Mouse, NTg and Tg, 8 - 12 week
Nelson, D. , Setser, E. , Hall, D. , Schwartz, S. , Hewitt, T. , Klevitsky, R. , Osinska, H. , Bellgrau, D. , Duke, R. and Robbins, J.
Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart ,
J Clin Invest
 105 (9) , 1199 , 2000
Cells
Myocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
Joklik's MEM
Heart
Mouse
Mouse
Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR.
High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates ,
Circulation
 101 , 178-84 , 2000
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
Joklik's MEM
Heart
Mouse
Mouse, 2-4 mo
Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.
Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
 279 , H429-36 , 2000
Cells
Ventricular myocytes
Enzymes
Protease XIV : 0.002%
Medium
MEM
Heart
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Heart
Mouse
Mouse, 1-3 day
Wang, G. and Kang, Y.
Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels ,
J Pharmacol Exp Ther
 288(3) , 938 , 1999
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.25%
Medium
FBS-MEM
Heart
Mouse
Mouse, 1 day
Lader, A. , Kwiatkowski, D. and Cantiello, H.
Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels ,
Am J Physiol
 277 , C1277 , 1999
Cells
Cardiomyocytes
Enzymes
NCIS kit : with modifications see reference
Medium
L-15
Heart
Mouse
Mouse, 1 day
Lader, A. , Xiao, Y. , Ishikawa, Y. , Cui, Y. , Vatner, D. , Vatner, S. , Homcy, C. and Cantiello, H.
Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway ,
Proc Natl Acad Sci U S A
 95 , 9669 , 1998
Cells
Cardiomyocytes
Enzymes
NCIS kit : with modifications see reference
Medium
L-15
Heart
Mouse
Mouse (BALB/c), adult
Felzen, B. , Shilkrut, M. , Less, H. , Sarapov, I. , Maor, G. , Coleman, R. , Robinson, R. and et al.
Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice ,
Circ Res
 82 , 438 , 1998
Cells
Myocytes
Enzymes
Medium
Tyrode's solution
Heart
Mouse
Mouse, adult
Grupp, I. , Lorenz, J. , Walsh, R. , Boivin, G. and Rindt, H.
Overexpression of Alpha1B-Adrenergic Receptor Induces Left Ventricular Dysfunction in the Absence of Hypertrophy. ,
Am J Physiol
 275 , H1338-50 , 1998
Cells
Cardiomyocytes
Enzymes
Medium
Joklik's MEM
Heart
Mouse
Mouse, P0-P1
Arber, S. , Hunter, J. , Ross, J. , Hongo, M. , Sansig, G. , Borg, J. , Perriard, J. , Chien, K. and Caroni, P.
MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure ,
Cell
 88 , 393 , 1997
Cells
Cardiomyocytes
Enzymes
Collagenase : 0.045%
Medium
DMEM, Medium 199
Heart
Mouse
Mouse, 6-9 or 21-41 day
Valenzuela, D. , Han, X. , Mende, U. , Fankhauser, C. , Mashimo, H. , Huang, P. , Pfeffer, J. , Neer, E. and Fishman, M.
G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart ,
Proc Natl Acad Sci U S A
 94(5) , 1727 , 1997
Cells
Ventricular and atrial myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Hyaluronidase : 172.5 u/ml
Trypsin : 0.002%
Medium
DMEM/Tyrodes
Heart
Mouse
Mouse
Soonpaa, M. , Kim, K. , Pajak, aL. , Franklin, M. and Field, L.
Cardiomyocyte DNA Synthesis and Binucleation During Murine Development ,
Am J Physiol
 271 , H2183 , 1996
Cells
Cardiomyocyte
Enzymes
Collagenase : 0.17 %
Medium
PBS
Heart
Mouse
Mouse embryos
Wobus, A. , Kleppisch, T. , Maltsev, V. and Hescheler, J.
Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels ,
In Vitro Cell Dev Biol
 30A , 425 , 1994
Cells
Myocytes
Enzymes
Medium
DMEM
Heart
Mouse
Mouse
(also rat, neonate and chick, 8 day embryo)
Gross, W. , Schopf-Ebner, E. and Bucher, O.
Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells ,
Exp Cell Res
 53 , 1 , 1968
Cells
Myocytes
Enzymes
Medium
Rinaldini's buffer solution, CF
Intestine
Mouse

Mouse

Kumar, S. , Suman, S. , Li, H. , Angdisen, J. , Datta, K. and Fornace, A.
High-linear Energy Transfer Radiation Disrupts Natural Killer Cell Surveillance of Senescent Intestinal Cells in the Mouse Intestine. , Mol Biomed Vol. 7 , 2026
Cells
Organoids
Enzymes
Medium
HBSS
Intestine
Mouse

Mouse, adult

Kumar, S. , Suman, S. , Angdisen, J. , Moon, B. , Kallakury, B. , Datta, K. and Fornace, A.
Effects of High-Linear-Energy-Transfer Heavy Ion Radiation on Intestinal Stem Cells: Implications for Gut Health and Tumorigenesis. ,
Cancers
 16 , 2024
Cells
Intestinal stem
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse
Emgard, J. , Kammoun, H. , Garcia-Cassani, B. , Chesne, J. , Parigi, S. , Jacob, J. , Cheng, H, Evren, E. , Das, S. , Czarnewski, P. , Sleiers, N. Melo-Gonzalez, F. , Kvedaraite, E. Svensson, M. and Scandella, E.
Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. ,
Immunity
 48 , 120-132.e8 , 2018
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse, embryonic
Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J. and Ming L
Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase. ,
Braz J Med Biol Res
 50 , e5831 , 2017
Cells
Crypt
Enzymes
Collagenase Type 1 : 200 u/ml
Hyaluronidase : 100u/ml
Medium
DMEM
Intestine
Mouse
Mouse
O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
 6 , 2016
Cells
Intestinal organoids
Enzymes
Medium
Basal Medium
Intestine
Mouse
Mouse
Seo, S. , Kuffa, P. , Kitamoto, S. , Nagao-Kitamoto, H. , Rousseau, J. , Kim, Y. , Nunez, G. and Kamada, N.
Intestinal Macrophages Arising from CCR2(+) Monocytes Control Pathogen Infection by Activating Innate Lymphoid Cells. ,
Nat Commun
 6 , 8010 , 2015
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse
Beura, L. , Anderson, K. , Schenkel, J. , Locquiao, J. , Fraser, K. , Vezys, V. , Pepper, M. and Masopust, D.
Lymphocytic Choriomeningitis Virus Persistence Promotes Effector-Like Memory Differentiation and Enhances Mucosal T cell Distribution. ,
J Leukoc Biol
 97 , 217 , 2015
Cells
Lymphocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
RPMI-1640
Intestine
Mouse
Zhang, Y. and Hu, W.
Mouse Enteric Neuronal Cell Culture. ,
Methods Mol Biol
 1078 , 55-63 , 2013
Cells
Enteric neurons
Enzymes
Trypsin : 0.05%
Deoxyribonuclease I : 400 u/ml
Medium
DMEM/F12
Intestine
Mouse
Mouse, P4
Hotta, R. , Stamp, L. , Foong, J. , McConnell, S. , Bergner, A. , Anderson, R. , Enomoto, H. , Newgreen, D. , Obermayr, F. , Furness, J. and Young, H.
Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon. ,
J Clin Invest
 123 , 1182 , 2013
Cells
Enteric neural crest progenitors
Enzymes
Medium
DMEM/F12
Intestine
Mouse
Mouse
Xue, X. and Shah, Y.
In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
 , e50210 , 2013
Cells
Colon tumor organoids
Enzymes
Medium
DMEM
Intestine
Mouse
Mouse
Geem, D. , Medina-Contreras, O. , Kim, W. , Huang, C. and Denning T.
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine. ,
J Vis Exp
 63 , e4040 , 2012
Cells
Dendritic, macrophages
Enzymes
Collagenase : 0.15%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.