For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Adipose/Fat
Hamster
Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, 5 week-12 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Hamster
Hamster (Mesocricetus auratus) 100-150 g
Liang, B.T. and Perlman, R.L.
Catecholamine Secretion by Hamster Adrenal Cells ,
J Neurochem
 32 , 927 , 1979
Cells
Adrenal
Chromaffin
Enzymes
Hyaluronidase : 0.20%
Medium
Kreb's Ringer bicarbonate buffer
Brain
Insect
Drosophilia
Hadzic, T. , Park, D. , Abruzzi, K. , Yang, L. , Trigg, J. , Rohs, R. , Rosbash, M. and Taghert, P.
Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED. ,
Nucleic Acids Res
 43 , 2199-215 , 2015
Cells
Neurons
Enzymes
Papain : 50 u/ml
Medium
Modified Dissecting Saline
Brain
Insect
Drosophila
Gu, H. and O'Dowd, D.
Whole Cell Recordings from Brain of Adult Drosophila. ,
J Vis Exp
 6 , 248 , 2007
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Saline
Brain
Insect
Drosophila
Sicaeros, B. Campusano, J. and O'Dowd, D.
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. ,
J Vis Exp
 4 , 200 , 2007
Cells
Neurons
Enzymes
Papain : 4 u/ml
Medium
DMEM
Brain
Insect
Drosophilia
Gu, H. and O'Dowd, D.
Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ. ,
J Neurosci
 26 , 265-72 , 2006
Cells
Kenyon
Enzymes
Papain : 20 u/ml
Medium
Recording saline
Brain
Monkey
Monkey, rhesus
Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS.
Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection ,
J Immunol
 167 , 5429-38 , 2001
Cells
Brain cells
Enzymes
Medium
HBSS
Epithelial
Hamster
Hamster, Syrian gold, male, 100-120 g
Niles,R. , Kim, K.C. , Hyman, B. , Christensen, T. , Wasano, K. and Brody, J.
Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells ,
In Vitro Cell Dev Biol
 24 , 457 , 1988
Cells
Tracheal
Enzymes
Pronase : 0.1%
Medium
MEM with Hepes, CMF
Epithelial
Hamster
Hamster, Syrian golden (strain CR:RGH)
McDowell, E. and et al.
Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters ,
Am J Pathol
 129 , 511 , 1987
Cells
Tracheal
Enzymes
Trypsin : 0.1%
Medium
Ham's F-12
Epithelial
Hamster
Hamster (strain 1516 EHS and Lakeview), 8-12 weeks
Lee, T. , Wu, R. , Brody, A. , Barrett, J. and Nettesheim, P.
Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture ,
Exp Lung Res
 6 , 27 , 1984
Cells
Tracheal
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Epithelial
Hamster
Hamster, Syrian gold, male, 6 weeks- 4 months
Goldman, W.E. and Baseman, J.B.
Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea ,
In Vitro
 16 , 313 , 1980
Cells
Tracheal
Enzymes
Trypsin : 0.25%
Medium
PBS with EDTA
Eye
Monkey
Macaques and baboon
Han ,Y. , Jacoby, R. and Wu, S.
Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells. ,
Brain Res
 875 , 175 , 2000
Cells
Retinal
Enzymes
Papain : 20-40 u/ml
Deoxyribonuclease I : 400 u/ml
Medium
Ames' solution
Eye
Monkey
Monkey, cynomolgus, young adult
Whittum-Hudson JA, Taylor HR.
Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection ,
Infect Immun
 57 , 2977 , 1989
Cells
Conjunctival lymphocytes
Enzymes
Medium
RPMI 1640
Kidney
Hamster
Hamster
(also rat, SD and Wistar, 150-225 g, rabbit, bovine)
Husted, R. , Hayashi, M. and Stokes, J.
Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
 255 , F1160 , 1988
Cells
Papillary duct
Enzymes
Hyaluronidase : 0.2%
Medium
Keri's buffer
HEPES buffered saline
Kidney
Monkey
Monkey
Melnick, J. and Wallis, C.
Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells ,
Dev Biol
 37 , 77 , 1976
Cells
Kidney
Enzymes
Trypsin : 0.01% - 0.00001%
Medium
Eagle's MEM
Liver
Monkey
Monkey, Macaca mulatta, 3-5.5 kg
Weber, A. and Groyer-Picard, M.and Dagher, I.
Hepatocyte Transplantation Techniques: Large Animal Models. ,
Methods Mol Biol
 481 , 83 , 2009
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 129 u/ml
Medium
DMEM/F12
Liver
Monkey
Monkey (Macaca fascicularis), adult, 5-6 Kg
Ulrich, R. , Aspar, D. , Cramer, C. , Kletzien, R. and Ginsberg, L.
Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis) ,
In Vitro Cell Dev Biol
 26 , 815 , 1990
Cells
Hepatocytes
Enzymes
Trypsin : 160 u/ml
Medium
HEPES buffer
Miscellaneous
Insect
Insect, Lepidoptera
Goodwin, R. and McCawley, P
Initiating Attached Cell Lines From the Lepidoptera (Insecta) ,
Meth Cell Sci
 3 , 567 , 1977
Cells
Lepitopteran
Enzymes
Medium
Dulbecco PBS
Muscle
Hamster
Hamster, male, 60-70 day
Nakamura, T. , Iwata, Y. , Sampaolesi, M. , Hanada, H. , Saito, N. , Artman, M. , Coetzee, W. and Shigekawa, M.
Stretch-Activated Cation Channels in Skeletal Muscle Myotubes From Sarcoglycan-Deficient Hamsters ,
Am J Physiol Cell Physiol
 281 , C690 , 2001
Cells
Satellite
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Monkey
Monkey (Macaca nemestrina)
Chait, A. , Ross, R. , Albers, J. and Bierman, E.
Platelet-Derived Growth Factor Stimulates Activity of LDL Receptors ,
Proc Natl Acad Sci U S A
 77 , 4084 , 1980
Cells
Smooth muscle
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt
Muscle
Monkey
Rhesus monkey, 1 year
(also human, rabbits)
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture ,
Cell Tissue Res
 177 , 503 , 1977
Cells
Smooth muscle, saphenous vein
Enzymes
Elastase : 0.05%
Medium
BSS
Neural
Hamster
Hamster, male
Liman ER.
Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons ,
J Physiol
 548 , 777 , 2003
Cells
Vomeronasal organ neurons
Enzymes
Trypsin : 0.02%
Medium
PBS
Neural
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Neural
Insect
Drosophila
Sanchez-Soriano, N. , Bottenberg, W. , Fiala, A. , Haessler, U. , Kerassoviti, A. , Knust, E. , Lohr, R. and Prokop, A.
Are Dendrites in Drosophila Homologous to Vertebrate Dendrites? ,
Dev Biol
 288 , 126 , 2005
Cells
Dendrites
Enzymes
Collagenase : 0.05%
Medium
HBSS
Neural
Insect
Gryllus Bimaculatus
Kloppenburg, P. and Horner, M.
Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus ,
J Exp Biol
 201 (Pt 17) , 2529 , 1998
Cells
Giant interneurons
Enzymes
Collagenase : 0.05%
Medium
Leibovitz's L15
Neural
Monkey
Macaque
Kupari, J. , Usoskin, D. , Parisien, M. , Lou, D. , Hu, Y. , Fatt, M. , Lonnerberg, P. , Spangberg, M. , Eriksson, B. , Barkas, N. , Kharchenko, P. , Lore, K, Khoury, S. , Diatchenko, L. and Ernfors, P.
Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain. ,
Nat Commun
 12 , 1510 , 2021
Cells
Dorsal root ganglia
Enzymes
Papain : 35 u/ml
Stemxyme 1 : 0.2%
Medium
see reference
Neural
Monkey

Rhesus macaque, female

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.
Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
 188 , 114520 , 2021
Cells
Dorsal root ganglia
Neural
Enzymes
Papain : 30 u/ml
Medium
HBSS
Pancreas
Hamster
Hamster
Feldman, J. and Chapman, B.
Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique ,
Acta Diabetol
 12 , 208 , 1975
Cells
Islets
Enzymes
Collagenase Type 4 : 1.3% - 2.0%
Medium
HBSS
Pancreas
Monkey
Monkey, 3-5 Kg
Scharp, D. , Murphy, J. , Newton, W. , Ballinger, W. and Lacy, P.
Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats ,
Transplant Proc
 7 , 739 , 1975
Cells
Islets
Enzymes
Hyaluronidase : 0.05%
Medium
HBSS
Reproductive
Hamster
Hamster, Chinese
Wallis, R. and Drickamer, K.
Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins ,
J Biol Chem
 274 (6) , 3580 , 1999
Cells
Ovary
Enzymes
Trypsin : 5%
Medium
Dialyzed fetal calf serum, 10% and 0.5M Methotrexate
Reproductive
Insect
Drosophila
Ma, X. , Wang, S. , Do, T. , Song, X. , Inaba, M. , Nishimoto, Y. , Liu, L. , Gao, Y. , Mao, Y. , Li, H. , McDowell, W. , Park, J. , Malanowski, K. , Peak, A. , Perera, A. and Li, H. et al.
Piwi is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary. ,
PLoS ONE
 9 , e90267 , 2014
Cells
Ovarian
Enzymes
Medium
Grace's
Reproductive
Insect
Drosophila, embryonic
Salmand, P. Iche-Torres, M. and Perrin, L.
Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis. , Fly (Austin) Vol. 5 , 261
Cells
Cardiac differentiating
Enzymes
Collagenase Type 1 : 20 u/ml
Trypsin : 0.25%
Medium
Schneider
Reproductive
Monkey
Rhesus macaque
Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB. and Stouffer, RL
Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin. ,
Hum Reprod
 26 , 1061-72 , 2011
Cells
Follicle
Enzymes
Medium
HEPES
Stem
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Stem
Monkey
Monkey
Chen, S. , Revoltella, R. , Papini, S. , Michelini, M. , Fitzgerald, W. and Zimmerberg, J.,and Margolis, L.
Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems ,
Stem Cells
 21(3) , 281 , 2003
Cells
Embryonic stem
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.