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Tissue References Search Tool

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Muscle
Rat
Rat, Wistar Kyoto, 10-15 weeks
McGuire, P. , Walker-Caprioglio, H. , Little, S. and McGuffee, L.
Isolation and Culture of Rat Superior Mesenteric Artery Smooth Muscle Cells ,
In Vitro Cell Dev Biol
 29 , 135 , 1993
Cells
Smooth muscle, mesenteric artery
Enzymes
Trypsin : 0.05%
Medium
MEM
Muscle
Rat
Rat
(also rabbit, guinea-pig)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Rat
Rat, SD, 19 days
Loch-Caruso, R. , Pahl, M. and Juberg, D.
Rat Myometrial Smooth Muscle Cells Show High Levels of Gap Junctional Communication Under a Variety of Culture Conditions ,
In Vitro Cell Dev Biol
 28 , 97 , 1992
Cells
Smooth muscle, myometrial
Enzymes
Trypsin : 150 µg/ml
Medium
HBSS or PSS, CMF
Muscle
Rat
Rat, SHRs and WKY, male, 10 - 14 weeks
Bolzon, B. and Cheung, D.
Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
 14 , 137 , 1989
Cells
Smooth muscle, tail arteries
Enzymes
Papain : 0.1%
Medium
HEPES buffer
(see reference)
Muscle
Rat
Rat, 1-3 day
Barone, L. , Wolfe, L. , Faris, B. and Franzblau, C.
Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat ,
Biochemistry
 27 , 3175 , 1988
Cells
Smooth muscle, aortic
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat
Bean, B. , Sturek, M. , Puga, A. and Hermsmeyer, K.
Calcium Channels in Muscle Cells Isolated From Rat Mesenteric Arteries: Modulation by Dihydropyridine Drugs ,
Circ Res
 59 , 229 , 1986
Cells
Muscle, mesenteric arteries
Enzymes
Trypsin : 0.05%
Medium
HEPES KG solution
(see reference)
Muscle
Rat
Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders
Gordon, D. , Mohai, L. and Schwartz, S.
Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells ,
Circ Res
 59 , 633 , 1986
Cells
Endothelial, aortic
Enzymes
Elastase : 0.05%
Medium
Waymouth's culture medium
Muscle
Rat
Rat, male, 150-250 g
Cole, O. , Fan, T. and Lewis, G.
Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta ,
Cell Biol Int Rep
 10 (6) , 399 , 1986
Cells
Endothelial, aortic
Medium
RPMI 1640
Muscle
Rat
Rat, SD, male, 200 - 250 g
Brock, T. , Alexander, R. , Ekstein, L. , Atkinson, W. and Gimbrone, M.
Angiotensin Increases Cytosolic Free Calcium in Cultured Vascular Smooth Muscle Cells ,
Hypertension
 7 , 105 , 1985
Cells
Smooth muscle, thoracic aorta
Enzymes
Trypsin : 0.0375%
Medium
Eagle's MEM with calcium
Muscle
Rat
Rat, Wistar, female, 10 weeks
Boulanger-Saunier, C. , Kattenburg, D. and Stoclet, J.
Cyclic AMP-dependent Phosphorylation of a 16kDa Protein in a Plasma Membrane-enriched Fraction of Rat Aortic Myocytes ,
F.E.B.S. Lett.
 193 , 283 , 1985
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
HBSS
Muscle
Rat
Rat, SD, 225-250g
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
 92 , 289 , 1982
Cells
Mesenteric artery smooth muscle cells
Enzymes
Medium
HBSS
Muscle
Rat
Rat, 3-4 day
Kasten, F.
Rat Myocardial Cells In Vitro: Mitosis and Differentiated Properties ,
In Vitro
 8 , 128 , 1972
Cells
Myocardial
Enzymes
Trypsin NF 1:250 : 0.125%
Medium
HBSS CMF
Muscle
Rat
Rat, 200 g
Kono, T.
Roles of Collagenases and Other Proteolytic Enzymes in the Dispersal of Animal Tissues ,
Biochim Biophys Acta
 178 , 397 , 1969
Cells
Muscle
Enzymes
Trypsin : 0.05%
Medium
Kreb's Henseleit bicarbonate buffer
Muscle
Rat
Rat, Wistar, 3-10 day
Harary, I. and Farley, B.
In Vitro Studies on Single Beating Rat Heart Cells ,
Exp Cell Res
 29 , 451 , 1963
Cells
Heart
Enzymes
Trypsin NF 1:250 : 250: 0.1%
Medium
Saline A
(See reference)
Neural
Human

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Human

Human

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.
Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
 188 , 114520 , 2021
Cells
Dorsal root ganglia
Neural
Enzymes
Papain : 30 u/ml
Medium
HBSS
Neural
Human
Human
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Neural
Human
Human
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Human
Human, 9 month
Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.
EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy ,
Nat Med
 11 , 277 , 2005
Cells
Neurons
Enzymes
Papain : 0.07 u/ml
Neutral Protease : 1 mg/ml
Medium
DMEM/F12
Neural
Human
Human, 26 week
Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.
Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells ,
J Neurosci Res
 75 , 782 , 2004
Cells
Neural progenitor cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Neural
Human
Human, adult
Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA.
Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone ,
J Neurosci Res
 59 , 321 , 2000
Cells
Ventricular epithelial
Enzymes
Papain : 11.4 u/ml
Medium
DMEM/F12
Neural
Human
Human, 5-65 years
Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
 69 , 97 , 1999
Cells
Retinal pigment epithelial (RPE)
Enzymes
Trypsin : 0.25%
Medium
HBSS
Neural
Human
Human
Dib-Hajj SD, Tyrrell L, Cummins TR, Black JA, Wood PM, Waxman SG.
Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons ,
F.E.B.S. Lett.
 462 , 117 , 1999
Cells
Dorsal root ganglion neurons
Enzymes
Medium
DMEM/F-12
Neural
Rat
Rat, 2-3 week
Lin, Y. and Chen, J.
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release. ,
J Vis Exp
 , 2018
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Rat
Rat, SD, 3 month
Andersen, N. and Monje, P.
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers. ,
Methods Mol Biol
 1739 , 49-66 , 2018
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, SD, day 2
Maurel, P.
Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies. ,
Methods Mol Biol
 1739 , 17-37 , 2018
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Rat
Rat, Wistar, 3 day
Fangmann, L. , Teller, S. , Stupakov, P. , Friess, H. , Ceyhan, G. and Demir, I.
3D Cancer Migration Assay with Schwann Cells. ,
Methods Mol Biol
 1739 , 317-325 , 2018
Cells
Dorsal root ganglia neurons
Enzymes
Medium
MEM
Neural
Rat
Rat, adult
George, D. , Ahrens, P. and Lambert, S.
Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells. ,
Glia
 , 2018
Cells
Schwann
Enzymes
Stemxyme 1 : 0.05%
Medium
DMEM
Neural
Rat
Rat, SD, p7
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, E17
Hayakawa, K. , Esposito, E. , Wang, X. , Terasaki, Y. , Liu, Y. , Xing, C. , Ji. X. and Lo, E.
Transfer of Mitochondria from Astrocytes to Neurons after Stroke. ,
Nature
 535 , 551-5 , 2016
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Rat
Rat, SD
Andersen, N. , Srinivas, S. , Pinero, G. and Monje, P.
A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves. ,
Sci Rep
 6 , 31781 , 2016
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, SD
Schuh, C. , Hercher, D. , Stainer, M. , Hopf, R. , Teuschl, A. , Schmidhammer, R. and Redl, H.
Extracorporeal Shockwave Treatment: A Novel Tool to Improve Schwann Cell Isolation and Culture. ,
Cytotherapy
 18 , 760-70 , 2016
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, 6-8 week
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Neural
Rat
Rat, embryonic
Oyanagi, K. , Tashiro, T. and Negishi, T.
Cell-Type-Specific and Differentiation-Status-Dependent Variations in Cytotoxicity of Tributyltin in Cultured Rat Cerebral Neurons and Astrocytes. ,
J Toxicol Sci
 40 , 459-68 , 2015
Cells
Cerebral neurons
Enzymes
Papain : 2 u/ml
Medium
DMEM-PBS
Neural
Rat
Rat, SD, 6-9 week
Murayama, C. , Watanabe, S. and Nakamura, M. and, Norimoto, H.
Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons. ,
Molecules
 20 , 14959-69 , 2015
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.