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Tissue References Search Tool

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Adrenal
Ovine
Ovine, adult and fetal
Valego, N. and Rose, J.
A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells. ,
Reprod Sci
 17 , 477 , 2010
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, fetal
Valego, N. , Su, Y. , Carey, L. , Young, S. , Tatter, S. , Wang, J. and Rose, J.
Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression. ,
Am J Physiol Regul Integr Comp Physiol
 289 , R410 , 2005
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, adult
Keating, D. , Rychkov, G. , Adams, M. , Holgert, H. , McMillen, I.C. and Roberts, M.
Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels. ,
J Physiol
 555 , 489 , 2004
Cells
Chromaffin cells
Enzymes
Medium
Locke's solution
Adrenal
Ovine
Ovine, 3 year
Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J.
The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep ,
J Endocrinol
 162 , 215-25 , 1999
Cells
Anterior pituitary
Enzymes
Trypsin : 2.5%
Medium
DMEM
Brain
Monkey
Monkey, rhesus
Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS.
Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection ,
J Immunol
 167 , 5429-38 , 2001
Cells
Brain cells
Enzymes
Medium
HBSS
Brain
Ovine
Ovine
Reddy, R. , Amodei, R. , Estill, C. , Stormshak, F. , Meaker, M. and Roselli, C.
Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture. ,
PLoS ONE
 10 , e0129521 , 2015
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Brain
Ovine
Ovine
Lepore G, Gadau S, Mura A, Zedda M, Farina V.
Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury. ,
Eur J Histochem
 53 , e28 , 2009
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Brain
Shellfish
Helisoma trivolvis
Hadley, R.D. , Bodnar, D.A. and Kater, S.B.
Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation ,
J Neurosci
 5 , 3145 , 1985
Cells
Neurons
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Saline, Sterile
Eye
Monkey
Macaques and baboon
Han ,Y. , Jacoby, R. and Wu, S.
Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells. ,
Brain Res
 875 , 175 , 2000
Cells
Retinal
Enzymes
Papain : 20-40 u/ml
Deoxyribonuclease I : 400 u/ml
Medium
Ames' solution
Eye
Monkey
Monkey, cynomolgus, young adult
Whittum-Hudson JA, Taylor HR.
Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection ,
Infect Immun
 57 , 2977 , 1989
Cells
Conjunctival lymphocytes
Enzymes
Medium
RPMI 1640
Heart
Ovine
Ovine
Voigt, N. , Pearman, C. , Dobrev, D. and Dibb, K.
Methods for Isolating Atrial Cells from Large Mammals and Humans. ,
J Mol Cell Cardiol
 86 , 187-98 , 2015
Cells
Atrial myocytes
Enzymes
Protease XIV : 0.006%
Medium
Tyrode's solution
Heart
Ovine
Ovine
Dibb, K. , Clarke, J. , Horn, M. , Richards, M. , Graham, H. , Eisner, D. and Trafford, A.
Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure. ,
Circ Heart Fail
 2 , 482-9 , 2009
Cells
Cardiomyocytes
Enzymes
Protease Type XIV : 0.006%
Medium
See reference
Heart
Ovine
Ovine
Dhamoon, A, Pandit, S. , Sarmast, F. , Parisian, K. , Guha, P. , Li, Y. , Bagwe, S. , Taffet, S. and Anumonwo, J.
Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. ,
Circ Res
 94 , 1332-9 , 2004
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 100-200 u/ml
Medium
See reference
Heart
Shellfish
Pacific Oysters
Le Duff, R. , Lipart, C. and Renault, T.
Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells ,
J Tiss Cul Meth
 16 , 67 , 1994
Cells
Haemocytes
Medium
L15 medium
Heart
Shellfish
Clams, 3.0 to 4.5 cm
Wen, C. , Kou, G. and Chen, S.
Cultivation of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding) ,
J Tiss Cul Meth
 15 , 123 , 1993
Cells
Myocytes
Fibroblasts
Enzymes
Medium
L15 medium
Kidney
Monkey
Monkey
Melnick, J. and Wallis, C.
Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells ,
Dev Biol
 37 , 77 , 1976
Cells
Kidney
Enzymes
Trypsin : 0.01% - 0.00001%
Medium
Eagle's MEM
Liver
Monkey
Monkey, Macaca mulatta, 3-5.5 kg
Weber, A. and Groyer-Picard, M.and Dagher, I.
Hepatocyte Transplantation Techniques: Large Animal Models. ,
Methods Mol Biol
 481 , 83 , 2009
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 129 u/ml
Medium
DMEM/F12
Liver
Monkey
Monkey (Macaca fascicularis), adult, 5-6 Kg
Ulrich, R. , Aspar, D. , Cramer, C. , Kletzien, R. and Ginsberg, L.
Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis) ,
In Vitro Cell Dev Biol
 26 , 815 , 1990
Cells
Hepatocytes
Enzymes
Trypsin : 160 u/ml
Medium
HEPES buffer
Muscle
Monkey
Monkey (Macaca nemestrina)
Chait, A. , Ross, R. , Albers, J. and Bierman, E.
Platelet-Derived Growth Factor Stimulates Activity of LDL Receptors ,
Proc Natl Acad Sci U S A
 77 , 4084 , 1980
Cells
Smooth muscle
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt
Muscle
Monkey
Rhesus monkey, 1 year
(also human, rabbits)
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture ,
Cell Tissue Res
 177 , 503 , 1977
Cells
Smooth muscle, saphenous vein
Enzymes
Elastase : 0.05%
Medium
BSS
Neural
Monkey
Macaque
Kupari, J. , Usoskin, D. , Parisien, M. , Lou, D. , Hu, Y. , Fatt, M. , Lonnerberg, P. , Spangberg, M. , Eriksson, B. , Barkas, N. , Kharchenko, P. , Lore, K, Khoury, S. , Diatchenko, L. and Ernfors, P.
Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain. ,
Nat Commun
 12 , 1510 , 2021
Cells
Dorsal root ganglia
Enzymes
Papain : 35 u/ml
Stemxyme 1 : 0.2%
Medium
see reference
Neural
Monkey

Rhesus macaque, female

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.
Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
 188 , 114520 , 2021
Cells
Dorsal root ganglia
Neural
Enzymes
Papain : 30 u/ml
Medium
HBSS
Neural
Shellfish
Aplysia californica
Lee, A. , Decourt, B. and Suter, D.
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones. ,
J Vis Exp
 12 , 662 , 2008
Cells
Neurons
Enzymes
Medium
L-15-ASW
Neural
Shellfish
Snails (Helisoma trivolis), albino, adult
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Neural
Shellfish
Pond snail (Helisoma) albino, adult
Haydon, P.
The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata ,
J Neurosci
 8 , 1032 , 1988
Cells
Somata, buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Antibiotic saline, Leibowitz 50%
Neural
Shellfish
Helisoma trivovlis
Cohan, C. , Haydon, P. and Kater, S.
Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma ,
J Neurosci Res
 13 , 285 , 1985
Cells
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
L-15 medium
Neural
Shellfish
Mollusc, juvenile 1-2 gm or adult 50-100 gm
Schacher, S. and Proshanksy, E.
Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment ,
J Neurosci
 3 (12) , 2403 , 1983
Cells
Neurons
LUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Neural
Shellfish
Aplysia californica
Camardo, J. , Proshansky, E. and Schacher, S.
Identified Aplysia Neurons Form Specific Chemical Synapses in Culture ,
J Neurosci
 3 (12) , 2614 , 1983
Cells
LUQ cells
RUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Pancreas
Monkey
Monkey, 3-5 Kg
Scharp, D. , Murphy, J. , Newton, W. , Ballinger, W. and Lacy, P.
Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats ,
Transplant Proc
 7 , 739 , 1975
Cells
Islets
Enzymes
Hyaluronidase : 0.05%
Medium
HBSS
Pituitary
Ovine
Ovine, adult
Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen.
Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes ,
Endocrinology
 143 , 4609-19 , 2002
Cells
Somatotropes
Enzymes
Medium
Medium 199
Reproductive
Monkey
Rhesus macaque
Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB. and Stouffer, RL
Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin. ,
Hum Reprod
 26 , 1061-72 , 2011
Cells
Follicle
Enzymes
Medium
HEPES
Reproductive
Ovine
Ovine, 3-5 year
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. ,
PLoS ONE
 10 , e0127531 , 2015
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Stem
Monkey
Monkey
Chen, S. , Revoltella, R. , Papini, S. , Michelini, M. , Fitzgerald, W. and Zimmerberg, J.,and Margolis, L.
Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems ,
Stem Cells
 21(3) , 281 , 2003
Cells
Embryonic stem
Enzymes
Medium
DMEM
Stem
Monkey
Baboons, 1yr, 7yr, 14 yr
Nagano, M. , McCarrey, J. and Brinster, R.
Primate Spermatogonial Stem Cells Colonize Mouse Testes ,
Biol Reprod
 64 , 1409 , 2001
Cells
Primate spermatogonial
Enzymes
Medium
DMEM
Stem
Ovine
Ovine, 3-5 year
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. ,
PLoS ONE
 10 , e0127531 , 2015
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.