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Tissue References Search Tool

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Endothelial
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.
Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
 3 , 2014
Cells
Cerebral artery endothelial
Enzymes
Neutral Protease : 4 u/ml
Elastase : 1 u/ml
Collagenase Type 1 : 120 u/ml
Medium
DMEM
Endothelial
Mouse
Mouse
Kazerounian, S. , Gerald, D. , Huang, M. , Chin, R, Udayakumar, D, Zheng, N. , O'Donnell, R. , Perruzzi, C. , Mangiante, L. , Pourat, J. , Phung, T. , Bravo-Nuevo, A. , Shechter, S. , McNamara, S. and Duhadaway, K.
RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis. ,
Cancer Res
 73 , 50 , 2013
Cells
Tumor endothelial
Enzymes
Medium
HBSS
Endothelial
Mouse
Mouse, 8-14 week
Imoukhuede, P. and Popel, A.
Expression of VEGF Receptors on Endothelial Cells in Mouse Skeletal Muscle. ,
PLoS ONE
 7 , e44791 , 2012
Cells
Endothelial
Enzymes
Medium
HBSS
Endothelial
Mouse
Mouse
Daneman, R. , Zhou, L. , Agalliu, D. , Cahoy, J. , Kaushal, A. and Barres, B.
The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells. ,
PLoS ONE
 5 , e13741 , 2010
Cells
Endothelial
Enzymes
Papain : 40 u/ml
Deoxyribonuclease I : 125 u/ml
Medium
DPBS
Endothelial
Mouse
Mouse, 7-10 week
Follenzi, A. , Benten, D. , Novikoff, P. , Faulkner, L. , Raut, S. and Gupta, S.
Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. ,
J Clin Invest
 118 , 935 , 2008
Cells
Liver endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Kondo, S. , Scheef, E. , Sheibani, N. and Sorenson, C.
PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis. ,
Am J Physiol Cell Physiol
 292 , C2070 , 2007
Cells
Endothelial kidney
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, embryonic or yolk sac
Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong.
Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation ,
J Cell Biol
 172 , 151-62 , 2006
Cells
Endothelial
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, male
Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K. and Miyasaka M
Expression profile of active genes in mouse lymph node high endothelial cells ,
Int Immunol
 11(12) , 1989 , 1999
Cells
Endothelial cells from lymph node
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, 2-4 week
Sapatino, B. , Welsh, C. , Smith, C. , Bebo, B. and Linticum, D.
Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme ,
In Vitro Cell Dev Biol
 29A , 923 , 1993
Cells
Cerebrovascular Endothelial
Enzymes
Medium
PBS
Endothelial
Rat
Rat, SD, male, 250-300 g
Schwertschlag, U.S. and Whorton, A.R.
Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells ,
J Biol Chem
 263 , 13791 , 1988
Cells
Smooth muscle, aorta
Enzymes
Medium
HBSS with 0.2 mM Ca++
Endothelial
Rat
Rat, SD, male, 350 - 450 g
Friedman, S. and Roll, F.
Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan ,
Anal Biochem
 161 , 207 , 1987
Cells
Lipocytes
Kupffer
Sinusoidal endothelial
Enzymes
Collagenase : 0.015%
Medium
DMEM/Ham's F-12
Endothelial
Rat
Rat, Wistar, male, 3 mo
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Endothelial
Rat
Rat, Wistar-Kyoto, male, 100 - 200 g
Diglio, C.A. , Grammas, P. , Filiberto Giacomelli, M.S. and Wiener, J.
Primary Culture of Rat Cerebral Microvascular Endothelial Cells ,
Lab Invest
 46 , 554 , 1982
Cells
Endothelial
Cerebral
Enzymes
Medium
HBSS
Endothelial
Rat
Rat, 300 G, and pig, 30-40 Kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Rat
Rat
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Endothelial
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS
Epithelial
Mouse
Mouse, 8-12 week
Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.
Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
 6 , 2020
Cells
Nasal epithelium
Enzymes
Papain : 20 u/ml
Medium
EBSS
Epithelial
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J.
Parent Stem Cells Can Serve as Niches for Their Daughter Cells. ,
Nature
 523 , 597-601 , 2015
Cells
Tracheal epithelial
Enzymes
Papain : 50 u/ml
Medium
See reference
Epithelial
Mouse
Mouse, adult
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1H.4 , 2011
Cells
Cilary epithelial
Enzymes
PDS kit : per instructions
Medium
EBSS
Epithelial
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Epithelial
Mouse
Mouse
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
 446 , 77-106 , 2008
Cells
Epithelial
Enzymes
Collagenase : 0.025%
Medium
DMEM
Epithelial
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Epithelial
Mouse
Mouse, female
Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y.
Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis ,
Am J Pathol
 155 , 173-81 , 1999
Cells
Salivary gland epithelial
Enzymes
Collagenase Type 1 : 750 u/ml
Hyaluronidase : 500 u/ml
Medium
DMEM/F12
Epithelial
Mouse
Mouse, fetal 12-13 day
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Mouse
Mouse
Durban, E.
Submandibular Salivary Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:2.1 , 1995
Cells
Submandibular salivary
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Katayama, M. and Kan, M.
Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation ,
In Vitro Cell Dev Biol
 27 , 533 , 1991
Cells
Esophageal
Enzymes
Trypsin : 0.25%
Medium
PBS, CMF
Epithelial
Mouse
Mouse, mature, female, 6-8-wk-old
Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.
Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes ,
In Vitro Cell Dev Biol
 27A , 713 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
HBSS
Epithelial
Mouse
Mouse, female, 18 - 20 days
(also 20 - 22 days)
Fukamachi, H. and McLachlan, J.
Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum ,
In Vitro Cell Dev Biol
 27A , 907 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.25%
Medium
HBSS
Epithelial
Mouse
Mouse, (BALB/c), male, 3-5 months
Durban, E.M.
Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix ,
In Vitro Cell Dev Biol
 26 , 33 , 1990
Cells
Epithelial
Submandibular salivary gland
Enzymes
Collagenase Type 3 : 0.16% , 1:1 v/v
Medium
DMEM with 15 mM HEPES
Epithelial
Mouse
Mouse, female
Riser, M. , Huff, B. and Medina, D.
Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
 19 , 730 , 1983
Cells
Epithelial
Enzymes
Pepsin : 0.1% and 0.05%
Pepsin : 0.1%
Medium
HBSS with 0.2% EDTA, CMF
Epithelial
Mouse
Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant
Yang, J. , Flynn, D. , Larson, L. and Hamamoto, S.
Growth in Primary Culture of Mouse Submandibular Epithelial Cells ,
In Vitro
 18 , 435 , 1982
Cells
Epithelial
Submandibular gland
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.