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Tissue References Search Tool

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Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Bone
Mouse
Mouse
Wang, W. , Majihail, G. , Lui, C. and Zhou, L.
Osteoblast Sorting and Intracellular Staining of CXCL12. ,
Bio Protoc
 8 , 2018
Cells
Osteoblast
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. ,
Stem Cells
 34 , 1692-701 , 2016
Cells
Bone-cartilage-stromal
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Igarashi, H. , Akahoshi, N. , Ohto-Nakanishi, T. , Yasuda, D. and Ishii, S.
The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells. ,
Sci Rep
 5 , 11410 , 2015
Cells
Bone stromal
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al.
Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts ,
Nature
 506 , 240 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse
Lee, D. , Choung, H. , Kim, H. , Gronostajski, R. , Yang, Y. , Ryoo, H. , Lee, Z. , Kim, H. , Cho, E. and Park, J.
NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. ,
Stem Cells
 32 , 2467-79 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse, 2-5 day
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Mouse
Mouse, 4-22 month
Stern, A. , Stern, M. , Van Dyke, M. , Jahn, K. , Prideaux, M. and Bonewald, L.
Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice. ,
Biotechniques
 52 , 361 , 2012
Cells
Osteocytes
Enzymes
Collagenase Type 1 : 300 u/ml
Medium
MEM
Bone
Mouse
Mouse
Nakamura, Y. , Arai, F. , Iwasaki, H. , Hosokawa, K. , Kobayashi, I. , Gomei, Y. , Matsumoto, Y. , Yoshihara, H. and Suda, T.
Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells. ,
Blood
 116 , 1422 , 2010
Cells
Endosteal cells
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Morikawa, S. , Mabuchi, Y. , Kubota, Y. , Nagai, Y. , Niibe, K. , Hiratsu, E. , Suzuki, S. , Miyauchi-Hara, C. , Nagoshi, N. , Sunabori, T. , Shimmura, S. , Miyawaki, A. , Nakagawa, T. , Suda, T. , Okano, H. and Matsuzaki, Y.
Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow. ,
J Exp Med
 206 , 2483-96 , 2009
Cells
Bone marrow
Enzymes
Collagenase : 0.2%
Medium
DMEM
Bone
Mouse
Mouse, male, 6-8 week old
Sakai, E. , Miyamoto, H. , Okamoto, K. , Kato, Y. , Yamamoto, K. and Sakai, H.
Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages ,
J. Biochem.
 130 , 823 , 2001
Cells
Osteoclasts
Enzymes
Medium
DMEM
Bone
Mouse
Mouse, 3-5 day, 6-8 week
Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M.
Thy-1 antigen Expression by Cells in the Osteoblast Lineage ,
J Bone Miner Res
 14 , 362 , 1999
Cells
Bone Cells, Osteoblasts
Enzymes
Collagenase Type 2 : 200-250 u/ml
Medium
DMEM
Bone
Mouse
Mouse, BALB/c
Takanashi, H. , Matsuishi, T. and Yoshizato, K.
Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells ,
In Vitro Cell Dev Biol
 30A , 384 , 1994
Cells
Osteoblast-like Cells
Stromal Cell Lines
Hematopoietic Blast Cells
Enzymes
Trypsin : 0.1%
Medium
Eagle's MEM
Bone
Mouse
Mouse, Swiss-Webster
Chen,T. and Feldman,D.
Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells ,
J Biol Chem
 256 , 5561 , 1981
Cells
Neonatal bone
Enzymes
Medium
Tris-buffered saline
Eye
Mouse

Mouse, 2 month

Murenu, E. , Pavlou, M. , Richter, L. , Rapti, K. , Just, S. and Cehajic-Kapetanovic, J.
A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species via Fluorescence-Activated Cell Sorting. , Mol Ther Methods Clin Dev Vol. 20 , 587-600 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Eye
Mouse
Mouse, 2-4 week
Liu, X. , Tang, L. and Liu Y.
Mouse Muller Cell Isolation and Culture. ,
Bio-protocol
 7 , 2017
Cells
Muller
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Mouse
Mouse
Ortin-Martinez, A. , Tsai, E. , Nickerson, P. , Bergeret, M. , Lu, Y. , Smiley, S. , Comanita, L. and Wallace, V.
A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors. ,
Stem Cells
 35 , 932-939 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
PBS
Eye
Mouse
Mouse
Wohl, S. , Jorstad, N. , Levine, E. and Reh, T.
Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture. ,
Nat Commun
 8 , 1603 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
HBSS
Eye
Mouse
Mouse, C57BL/6
Blandford, S. and Baldridge, W.
The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i. ,
Biomed Res Int
 2016 , 8178162 , 2016
Cells
Retinal astrocytes
Enzymes
Papain : 16.5 u/ml
Medium
HBSS
Eye
Mouse
Mouse, adult
Feodorova, Y. , Koch, M. , Bultman, S. , Michalakis, S. and Solovei, I.
Quick and Reliable Method for Retina Dissociation and Separation of Rod Photoreceptor Perikarya from Adult Mice. ,
MethodsX
 2 , 39 , 2015
Cells
Retinal photoreceptor
Enzymes
PDS kit : with modifications
Medium
EBSS
Eye
Mouse
Mouse, 4-8 day
Balmer, J. , Zulliger, R. , Roberti, S. and Enzmann, V.
Retinal Cell Death Caused by Sodium Iodate Involves Multiple Caspase-Dependent and Caspase-Independent Cell-Death Pathways. ,
Int J Mol Sci
 16 , 15086-103 , 2015
Cells
Photoreceptors
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Eye
Mouse
Mouse, embryonic and P0
Dvoriantchikova, G. , Perea-Martinez, I. , Pappas, S. , Barry, A. , Danek, D. , Dvoriantchikova, X. , Pelaez, D. and Ivanov, D.
Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina. ,
PLoS ONE
 10 , e0131054 , 2015
Cells
Retinal
Enzymes
Papain : 8 u/ml
Deoxyribonuclease I : 124 u/ml
Medium
DPBS
Eye
Mouse
Mouse
Iqbal, N. , Xu, L. , Devitt, C. and Skapek, S.
Isolation and Characterization of Mammalian Cells Expressing the Arf Promoter During Eye Development. ,
Biotechniques
 56 , 239-49 , 2014
Cells
Vitreous cells
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.03%
Medium
DMEM
Eye
Mouse
Mouse, 6 week
Singh, M. , Issa, P, Butler, R. , Martin, C. , Lipinski, D. , Sekaran, S. , Barnard, A. and Maclaren, R.
Reversal of End-Stage Retinal Degeneration and Restoration of Visual Bunction by Photoreceptor Transplantation. ,
Proc Natl Acad Sci U S A
 , 2013
Cells
Retinal
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Mouse
Mouse, adult
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1H.4 , 2011
Cells
Cilary epithelial
Enzymes
PDS kit : per instructions
Medium
EBSS
Eye
Mouse
Mouse
Schmidt, T. and Kofuji, P.
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells. ,
J Vis Exp
 47 , 2367 , 2011
Cells
Retinal ganglion
Enzymes
CLSPA : 240 u/ml
Hyaluronidase : 0.2%
Medium
Ames
Eye
Mouse
Mouse, 4-5 week
Ebihara, L. , Tong, J. , Vertel, B. , White, T. and Chen, T.
Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells. ,
Inv Ophthal Visual Sci
 52 , 882-9 , 2011
Cells
Lens Fiber
Enzymes
Protease : 0.5%
Medium
PBS
Eye
Mouse
Mouse, postnatal day 1
Jiang, C. Klassen, H. , Zhang, X. and Young, M.
Laser Injury Promotes Migration and Integration of Retinal Progenitor Cells into Host Retina. ,
Mol Vis
 16 , 983 , 2010
Cells
Retinal progenitor
Enzymes
Collagenase : 0.1%
Medium
HBSS
Eye
Mouse
Mouse, 4-8 week
Kobayashi, T. , Yoshioka, R. , Shiraishi, A. and Ohashi, Y.
New Technique for Culturing Corneal Epithelial Cells of Normal Mice. ,
Mol Vis
 15 , 1589-93 , 2009
Cells
Corneal epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Eye
Mouse
Mouse
Amadi-Obi, A. , Yu, C. , Liu, X. , Mahdi, R. , Clarke, G. , Nussenblatt, R, Gery, I. , Lee, Y. and Egwuagu, C.
TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1. ,
Nat Med
 13 , 711 , 2007
Cells
Retinal
Enzymes
Medium
RPMI
Eye
Mouse
mouse, adult
Jadhav AP, Cho SH. and Cepko CL
Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property ,
Proc Natl Acad Sci U S A
 103 , 18998 , 2006
Cells
Retinal
Enzymes
Papain : 50 u/ml
Medium
DMEM/F-12
Eye
Mouse
Mouse, adult
Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K.
Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. ,
Stem Cells
 24 , 2714-22 , 2006
Cells
Stromal
Enzymes
Trypsin : 0.05%
Collagenase : 78 u/ml
Hyaluronidase : 38 u/ml
Medium
DMEM/F-12
Eye
Mouse
Mouse, 2-5 month
Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto.
Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission ,
J Neurosci
 25 , 566-76 , 2005
Cells
Mouse retinal and bipolar
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
HBSS
Eye
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.