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Tissue References Search Tool

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Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Bone
Mouse
Mouse
Wang, W. , Majihail, G. , Lui, C. and Zhou, L.
Osteoblast Sorting and Intracellular Staining of CXCL12. ,
Bio Protoc
 8 , 2018
Cells
Osteoblast
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. ,
Stem Cells
 34 , 1692-701 , 2016
Cells
Bone-cartilage-stromal
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Igarashi, H. , Akahoshi, N. , Ohto-Nakanishi, T. , Yasuda, D. and Ishii, S.
The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells. ,
Sci Rep
 5 , 11410 , 2015
Cells
Bone stromal
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al.
Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts ,
Nature
 506 , 240 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse
Lee, D. , Choung, H. , Kim, H. , Gronostajski, R. , Yang, Y. , Ryoo, H. , Lee, Z. , Kim, H. , Cho, E. and Park, J.
NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. ,
Stem Cells
 32 , 2467-79 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse, 2-5 day
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Mouse
Mouse, 4-22 month
Stern, A. , Stern, M. , Van Dyke, M. , Jahn, K. , Prideaux, M. and Bonewald, L.
Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice. ,
Biotechniques
 52 , 361 , 2012
Cells
Osteocytes
Enzymes
Collagenase Type 1 : 300 u/ml
Medium
MEM
Bone
Mouse
Mouse
Nakamura, Y. , Arai, F. , Iwasaki, H. , Hosokawa, K. , Kobayashi, I. , Gomei, Y. , Matsumoto, Y. , Yoshihara, H. and Suda, T.
Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells. ,
Blood
 116 , 1422 , 2010
Cells
Endosteal cells
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Morikawa, S. , Mabuchi, Y. , Kubota, Y. , Nagai, Y. , Niibe, K. , Hiratsu, E. , Suzuki, S. , Miyauchi-Hara, C. , Nagoshi, N. , Sunabori, T. , Shimmura, S. , Miyawaki, A. , Nakagawa, T. , Suda, T. , Okano, H. and Matsuzaki, Y.
Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow. ,
J Exp Med
 206 , 2483-96 , 2009
Cells
Bone marrow
Enzymes
Collagenase : 0.2%
Medium
DMEM
Bone
Mouse
Mouse, male, 6-8 week old
Sakai, E. , Miyamoto, H. , Okamoto, K. , Kato, Y. , Yamamoto, K. and Sakai, H.
Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages ,
J. Biochem.
 130 , 823 , 2001
Cells
Osteoclasts
Enzymes
Medium
DMEM
Bone
Mouse
Mouse, 3-5 day, 6-8 week
Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M.
Thy-1 antigen Expression by Cells in the Osteoblast Lineage ,
J Bone Miner Res
 14 , 362 , 1999
Cells
Bone Cells, Osteoblasts
Enzymes
Collagenase Type 2 : 200-250 u/ml
Medium
DMEM
Bone
Mouse
Mouse, BALB/c
Takanashi, H. , Matsuishi, T. and Yoshizato, K.
Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells ,
In Vitro Cell Dev Biol
 30A , 384 , 1994
Cells
Osteoblast-like Cells
Stromal Cell Lines
Hematopoietic Blast Cells
Enzymes
Trypsin : 0.1%
Medium
Eagle's MEM
Bone
Mouse
Mouse, Swiss-Webster
Chen,T. and Feldman,D.
Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells ,
J Biol Chem
 256 , 5561 , 1981
Cells
Neonatal bone
Enzymes
Medium
Tris-buffered saline
Neural
Mouse

Mouse, 4-6 week

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Mouse

Mouse

Yousuf, M. , Moreno, M. , Li, J. , He, L. , Royer, D. , Zhang, J. , Woodall, B. , Grace, P. and Price, T.
Diroximel fumarate acts hrough Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons. ,
bioRxivorg
 Version 1 , Preprint Dec 23 , 2023
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Mouse

Mouse, adult

Golan, N. and Cafferty, W.
Dissociation of Intact Adult Mouse Cortical Projection Neurons for Single-Cell RNA-seq. ,
Star Protocols
 2 , 100941 , 2021
Cells
Cortical neurons
Enzymes
Papain : 13 u/ml
Medium
Artifical Cerebrospinal Fluid
Neural
Mouse
Mouse
Yousuf, M. , Samtleben, S. , Lamothe, S. , Friedman, T. , Catuneanu, A. and et al.
ER Stress in the Dorsal Root Ganglia Regulates BK Channel Physiology and Contributes to Pain Hypersensitivity in a Mouse Model of Multiple Schlerosis ,
bioRxivorg
 , 2020
Cells
Dorsal root ganglion
Enzymes
Stemxyme 1 : 0.1-0.2%
Medium
HBSS
Neural
Mouse
Mouse, embryonic
Kramer, N. , Haney, M. , Morgens, D. , Jovicic, A. , Couthouis, J. , Li, A. , Ousey, J. , Ma, R. , Bieri, G. , Tsui, C. , Shi, Y. , Hertz, N. , Tessier-Lavigne, M. , Ichida, J. , Bassik, M. and Gitler, A.
CRISPR-Cas9 Screens in Human Cells and Primary Neurons Identify Modifiers of C9ORF72 Dipeptide-Repeat-Protein Toxicity. ,
Nat Genet
 , 2018
Cells
Cortical neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Neural
Mouse
Mouse
Meas, S. , Nishimura, K. , Scheibinger, M. and Dabdoub, A.
,
Front Neurosci
 12 , 822 , 2018
Cells
Spiral ganglion
Enzymes
Medium
MEM
Neural
Mouse
Mouse, adult
Weng, Y. , An, R. , Cassin, J. , Joseph, J. , Mi, R. , Wang, C. , Zhong, C. , Jin, S. , Pfeifer, G. , Bellacosa, A. , Dong, X. , Hoke, A. , He, Z. , Song, H. and Ming, G.
An Intrinsic Epigenetic Barrier for Functional Axon Regeneration ,
Neuron
 94 , 337 , 2018
Cells
Lumbar DRG
Enzymes
Collagenase Type 2 : 200 u/ml
Neutral Protease : 2.5 u/ml
Medium
HBSS
Neural
Mouse
Mouse, embryonic 12.5
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Mouse
Mouse, P2-P12
Fangmann, L. , Teller, S. , Stupakov, P. , Friess, H. , Ceyhan, G. and Demir, I.
3D Cancer Migration Assay with Schwann Cells. ,
Methods Mol Biol
 1739 , 317-325 , 2018
Cells
Dorsal root ganglia neurons
Enzymes
Medium
MEM
Neural
Mouse
Mouse
Xu, W. , Sachewsky, N. , Azimi, A. , Hung, M. , Gappasov, A. and Morshead, C.
Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. ,
Stem Cells
 35 , 485-496 , 2017
Cells
Neurospheres
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.08%
Medium
Neurobasal A
Neural
Mouse
Mouse, day 2
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Mouse
Mouse, 4 month
Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.
Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
 7 , 13915 , 2017
Cells
Satellite glial
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, 2-3 month
Sun, H. , Tsai, W. , Li, B. , Tao, W. , Chen, P. and Rubart, M.
Voltage-Induced Calcium Release in Postganglionic Sympathetic Neurons in Adult Mice. ,
PLoS ONE
 11 , e0148962 , 2016
Cells
Sympathetic neurons
Enzymes
Papain : 10 u/ml
Medium
EBSS
Neural
Mouse
Mouse
Beaudet, M. , Yang, Q. , Cadau, S. , Blais, M. , Bellenfant, S. , Gros-Louis, F. and Berthod, F.
High Yield Extraction of Pure Spinal Motor Neurons, Astrocytes and Microglia from Single Embryo and Adult Mouse Spinal Cord. ,
Sci Rep
 5 , 16763 , 2015
Cells
Spinal motor neurons, astrocytes, microglia
Enzymes
Papain : 0.2%
Medium
HBSS
Neural
Mouse
Mouse
Ren, H. , Lu, T. , McGraw, T. and Accili, D.
Anorexia and Impaired Glucose Metabolism in Mice with Hypothalamic Ablation of Glut4 Neurons. ,
Diabetes
 64 , 405-17 , 2015
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
EBSS
Neural
Mouse
Mouse, adult
Choi, H. , Sun, D. and Jakobs, T.
Isolation of Intact Astrocytes from the Optic Nerve Head of Adult Mice. ,
Exp Eye Res
 137 , 103-10 , 2015
Cells
Astrocytes
Enzymes
Papain : ~20 u/ml
Medium
HBSS
Neural
Mouse
Mouse
Harris, M. , Hulseberg, P. , Ling, C. , Karman, J. , Clarkson, B. , Harding, J. , Zhang, M. , Sandor, A. , Christensen, K. , Nagy, A. , Sandor, Matyas,S. and Fabry, Z.
Immune Privilege of the CNS is not the Consequence of Limited Antigen Sampling. ,
Sci Rep
 4 , 4422 , 2014
Cells
Oligodendrocytes
Enzymes
Papain : 0.1%
Medium
HBSS
Neural
Mouse
Mouse
Williams, E. , Zhong, X. , Mohamed, A. , Li, R. , Liu, Y. , Dong, Q. , Ananiev, G. , Mok, J. , Lin, B. , Lu, J. , Chiao, C. , Cherney, R. , Li, H. , Zhang, S. and Chang, Q
Mutant Astrocytes Differentiated from Rett Syndrome Patients-Specific iPSCs Have Adverse Effects on Wild-Type Neurons. ,
Hum Mol Genet
 23 , 2968-80 , 2014
Cells
Hippocampal neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Mouse
Mouse
Stuart, M. , Corrigan, F. and Baune, B.
Knockout of CXCR5 Increases the Population of Immature Neural Cells and Decreases Proliferation in the Hippocampal Dentate Gyrus. ,
J Neuroinflammation
 11 , 31 , 2014
Cells
Neurosperes
Enzymes
Papain : 0.1%
Medium
NeuroCult NSC
Neural
Mouse
Mouse, neonatal
Stettner, M. , Lohmann, B. , Wolffram, K. , Weinberger, J. , Dehmel, T. , Hartung, H. , Mausberg, A. and Kieseier, B.
Interleukin-17 Impedes Schwann Cell-Mediated Myelination. ,
J Neuroinflammation
 11 , 63 , 2014
Cells
Schwann cells
Enzymes
Collagenase : 0.05-0.1%
Trypsin : 0.125-0.25%
Medium
DMEM
Neural
Mouse
Mouse, 2 month
Liu, G. , Rustom, N. , Litteljohn, D. , Bobyn, J. , Rudyk, C. , Anisman, H. and Hayley, S.
Use of Induced Pluripotent Stem Cell Derived Neurons Engineered to Express BDNF for Modulation of Stressor Related Pathology. ,
Front Cell Neurosci
 8 , 316 , 2014
Cells
Fibroblasts
Enzymes
Papain : 0.2%
Medium
Hibernate

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.