For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Cartilage
Mouse
Mouse, 2-5 day
Otsuru, S. , Hofmann, T. , Raman, P. , Olson, T. , Guess, A. , Dominici, M. and Horwitz, E.
Genomic and Functional Comparison of Mesenchymal Stromal Cells Prepared Using Two Isolation Methods. ,
Cytotherapy
 17 , 262-70 , 2015
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Collagenase Type 1 : 86.5 u/ml
Medium
DMEM
Cartilage
Mouse
Mouse, 1 day
Terpstra, L, Prud'homme, J, Arabian, A, Takeda, S, Karsenty, G, Dedhar, S. and St-Arnaud, R.
Reduced Chondrocyte Proliferation and Chondrodysplasia in Mice Lacking the Integrin-linked Kinase in Chondrocytes ,
J Cell Biol
 162 , 139 , 2003
Cells
Chondrocytes
Enzymes
Collagenase : 0.2%
Medium
DMEM
Intestine
Mouse

Mouse

Kumar, S. , Suman, S. , Li, H. , Angdisen, J. , Datta, K. and Fornace, A.
High-linear Energy Transfer Radiation Disrupts Natural Killer Cell Surveillance of Senescent Intestinal Cells in the Mouse Intestine. , Mol Biomed Vol. 7 , 2026
Cells
Organoids
Enzymes
Medium
HBSS
Intestine
Mouse

Mouse, adult

Kumar, S. , Suman, S. , Angdisen, J. , Moon, B. , Kallakury, B. , Datta, K. and Fornace, A.
Effects of High-Linear-Energy-Transfer Heavy Ion Radiation on Intestinal Stem Cells: Implications for Gut Health and Tumorigenesis. ,
Cancers
 16 , 2024
Cells
Intestinal stem
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse
Emgard, J. , Kammoun, H. , Garcia-Cassani, B. , Chesne, J. , Parigi, S. , Jacob, J. , Cheng, H, Evren, E. , Das, S. , Czarnewski, P. , Sleiers, N. Melo-Gonzalez, F. , Kvedaraite, E. Svensson, M. and Scandella, E.
Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. ,
Immunity
 48 , 120-132.e8 , 2018
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse, embryonic
Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J. and Ming L
Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase. ,
Braz J Med Biol Res
 50 , e5831 , 2017
Cells
Crypt
Enzymes
Collagenase Type 1 : 200 u/ml
Hyaluronidase : 100u/ml
Medium
DMEM
Intestine
Mouse
Mouse
O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
 6 , 2016
Cells
Intestinal organoids
Enzymes
Medium
Basal Medium
Intestine
Mouse
Mouse
Seo, S. , Kuffa, P. , Kitamoto, S. , Nagao-Kitamoto, H. , Rousseau, J. , Kim, Y. , Nunez, G. and Kamada, N.
Intestinal Macrophages Arising from CCR2(+) Monocytes Control Pathogen Infection by Activating Innate Lymphoid Cells. ,
Nat Commun
 6 , 8010 , 2015
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse
Beura, L. , Anderson, K. , Schenkel, J. , Locquiao, J. , Fraser, K. , Vezys, V. , Pepper, M. and Masopust, D.
Lymphocytic Choriomeningitis Virus Persistence Promotes Effector-Like Memory Differentiation and Enhances Mucosal T cell Distribution. ,
J Leukoc Biol
 97 , 217 , 2015
Cells
Lymphocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
RPMI-1640
Intestine
Mouse
Zhang, Y. and Hu, W.
Mouse Enteric Neuronal Cell Culture. ,
Methods Mol Biol
 1078 , 55-63 , 2013
Cells
Enteric neurons
Enzymes
Trypsin : 0.05%
Deoxyribonuclease I : 400 u/ml
Medium
DMEM/F12
Intestine
Mouse
Mouse, P4
Hotta, R. , Stamp, L. , Foong, J. , McConnell, S. , Bergner, A. , Anderson, R. , Enomoto, H. , Newgreen, D. , Obermayr, F. , Furness, J. and Young, H.
Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon. ,
J Clin Invest
 123 , 1182 , 2013
Cells
Enteric neural crest progenitors
Enzymes
Medium
DMEM/F12
Intestine
Mouse
Mouse
Xue, X. and Shah, Y.
In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
 , e50210 , 2013
Cells
Colon tumor organoids
Enzymes
Medium
DMEM
Intestine
Mouse
Mouse
Geem, D. , Medina-Contreras, O. , Kim, W. , Huang, C. and Denning T.
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine. ,
J Vis Exp
 63 , e4040 , 2012
Cells
Dendritic, macrophages
Enzymes
Collagenase : 0.15%
Medium
HBSS
Intestine
Mouse
Mouse, 2-3 month
Becker, L. , Kulkarni, S. , Tiwari, G. , Micci, M. and Pasricha, P.
Divergent Fate and Origin of Neurosphere-Like Bodies from Different Layers of the Gut. ,
Am J Physiol Gastrointest Liver Physiol
 302 , G958-65 , 2012
Cells
Enteric neural stem cells
Enzymes
Medium
M199
Intestine
Mouse
Mouse
Barthel, E. , Speer, A. , Levin, D. , Sala, F. , Hou, X. , Torashima, Y. , Wigfall, C. and Grikscheit, T.
Tissue Engineering of the Intestine in a Murine Model. ,
J Vis Exp
 70 , e4279 , 2012
Cells
Intestinal organoids
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Intestine
Mouse
Mouse
Ruiz, V. , Sachdev, M. , Zhang, S. , Wen, S. and Moss, S.
Isolating, Immunophenotyping and Ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes During Murine Helicobacter Pylori Infection. ,
J Immunol Methods
 384 , 157-63 , 2012
Cells
Lyphocytes
Enzymes
Medium
RPMI 1640
Intestine
Mouse
Mouse, neonatal and adult
Joseph, N. , He, S. , Quintana, E. , Kim, Y. , Nunez, G. and Morrison, S.
Enteric Glia are Multipotent in Culture but Primarily Form Glia in the Adult Rodent Gut. ,
J Clin Invest
 121 , 3398 , 2011
Cells
Myenteric plexus
Enzymes
Collagenase Type 4 : 0.025-0.1%
Papain : 10 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
HBSS
Intestine
Mouse
Mouse
Ito, Y. , Kanai, T. , Totsuka, T. , Okamoto, R. , Tsuchiya, K. , Nemoto, Y. , Yoshioka, A. , Tomita, T. , Nagaishi, T. , Sakamoto, N. , Sakanishi, T. , Okumura, K. , Yagita, H. and Watanabe, M.
Blockade of NKG2D Signaling Prevents the Development of Murine CD4+ T Cell-Mediated Colitis. ,
Am J Physiol Gastrointest Liver Physiol
 294 , G199 , 2008
Cells
Lamina propria lymphocytes
Enzymes
Medium
RPMI 1640
Intestine
Mouse
Mouse
Forbes, E. , Groschwitz, K. , Abonia, J.P. , Brandt, E. , Cohen, E. , Blanchard, C. , Ahrens, R. , Seidu, L. , McKenzie, A. , Strait, R. , Finkelman, F. , Foster, P. , Matthaei, K. , Rothenberg, M. and Hogan, S.
IL-9- and Mast Cell-Mediated Intestinal Permeability Predisposes to Oral Antigen Hypersensitivity. ,
J Exp Med
 205 , 897 , 2008
Cells
Intestinal mononuclear
Enzymes
Medium
RPMI 1640
Intestine
Mouse
Mouse, Mastomys natalensis
Kidd, M. , Modlin, I. , Eick, G. and Champaneria, M.
Isolation, Functional Characterization, and Transcriptome of Mastomys Ileal Enterochromaffin Cells. ,
Am J Physiol Gastrointest Liver Physiol
 291 , G778-91 , 2006
Cells
Enterochromaffin cells
Enzymes
Collagenase : 0.025%
Pronase E : 0.07%
Medium
HBSS
Intestine
Mouse
Mouse, 7 week
Goto Kazunori, Matsuoka Satoshi, Noma Akinori.
Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine ,
J Physiol
 559 , 411-22 , 2004
Cells
Interstitial cells
Enzymes
Collagenase : 0.04%
Trypsin : 0.02%
Medium
see reference
Intestine
Mouse
Mouse, 9-13 day
Ordag Tamas, Redelman Doug, Miller Lisa J, Horvath Viktor J, Zhong Qiao, Almeida-Porada Graca, Zanjani Esmail D, Horowitz Burton, Sanders Kenton M.
Purification of interstitial cells of Cajal by fluorescence-activated cell sorting ,
Am J Physiol Cell Physiol
 286 , C448-56 , 2004
Cells
Interstitial cells of Cajal
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse, 0-15 day
Lee Young Mee, Kim Byung Joo, Kim Hyun Jin, Yang Dong Ki, Zhu Mei Hong, Lee Kyu Pil, So Insuk, Kim Ki Whan.
TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach ,
Am J Physiol/Gastro
 284 , G604-16 , 2003
Cells
Interstitial cells of Cajal
Enzymes
Medium
Hanks
Intestine
Mouse
Mouse
Wu Y, Wang X, Csencsits KL, Haddad A, Walters N, Pascual DW.
M cell-targeted DNA vaccination ,
Proc Natl Acad Sci U S A
 98 , 9318-23 , 2001
Cells
Lamina propria lymphocytes
Enzymes
Collagenase Type 4 : 300 u/ml
Medium
PBS
Intestine
Mouse
Mouse, F14
Sakagami Y, Inaguma Y, Sakakura T, Nishizuka Y.
Intestine-like remodeling of adult mouse glandular stomach by implanting of fetal intestinal mesenchyme ,
Cancer Res
 44 , 5845-9 , 1984
Cells
Intestinal mesenchymal
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.