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Tissue References Search Tool

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Endothelial
Mouse
Mouse, 4 week
Esteban, S. , Clemente, C. , Koziol, A. , Gonzalo, P. , Rius, C. , Martinez, F. , Linares, P. , Chaparro, M. , Urzainqui, A. , Andres, V. , Seiki, M. , Gisbert, J. and Arroyo, A.
Endothelial MT1-MMP Targeting Limits Intussusceptive Angiogenesis and Colitis via TSP1/Nitric Oxide Axis. ,
EMBO Mol Med
 12 , e10862 , 2020
Cells
Aortic endothelial
Enzymes
Elastase : 0.25%
Medium
DMEM
Endothelial
Mouse
Mouse, 2 month
Sabbagh, M. and Nathans, J.
A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture ,
Elife
 , 1 , 2020
Cells
Endothelial, CNS
Enzymes
PDS kit : with modifications
Stemxyme 1 : 0.1%
Medium
EC growth medium
Endothelial
Mouse
Mouse
Luo, S. , Truong, A. and Makino, A.
Isolation of Mouse Coronary Endothelial Cells. ,
J Vis Exp
 , 2016
Cells
Coronary endothelial
Enzymes
Medium
Kreb's
Endothelial
Mouse
Mouse
Wang, J. , Sun, C. , Gerdes, N. , Liu, C. , Liao, M. , Liu, J. , Shi, M. , He, A. , Zhou, Y. , Sukhova, G. , Chen, H. , Cheng, X. , Kuzuya, M. , Murohara, T. , Zhang, J. , Cheng, X. , Jiang, M. , Shull, G. and Rogers, S.
Interleukin 18 Function in Atherosclerosis is Mediated by the Interleukin 18 Receptor and the Na-Cl Co-Transporter. ,
Nat Med
 21 , 820-6 , 2015
Cells
Endothelial lung
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.
Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
 3 , 2014
Cells
Cerebral artery endothelial
Enzymes
Neutral Protease : 4 u/ml
Elastase : 1 u/ml
Collagenase Type 1 : 120 u/ml
Medium
DMEM
Endothelial
Mouse
Mouse
Kazerounian, S. , Gerald, D. , Huang, M. , Chin, R, Udayakumar, D, Zheng, N. , O'Donnell, R. , Perruzzi, C. , Mangiante, L. , Pourat, J. , Phung, T. , Bravo-Nuevo, A. , Shechter, S. , McNamara, S. and Duhadaway, K.
RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis. ,
Cancer Res
 73 , 50 , 2013
Cells
Tumor endothelial
Enzymes
Medium
HBSS
Endothelial
Mouse
Mouse, 8-14 week
Imoukhuede, P. and Popel, A.
Expression of VEGF Receptors on Endothelial Cells in Mouse Skeletal Muscle. ,
PLoS ONE
 7 , e44791 , 2012
Cells
Endothelial
Enzymes
Medium
HBSS
Endothelial
Mouse
Mouse
Daneman, R. , Zhou, L. , Agalliu, D. , Cahoy, J. , Kaushal, A. and Barres, B.
The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells. ,
PLoS ONE
 5 , e13741 , 2010
Cells
Endothelial
Enzymes
Papain : 40 u/ml
Deoxyribonuclease I : 125 u/ml
Medium
DPBS
Endothelial
Mouse
Mouse, 7-10 week
Follenzi, A. , Benten, D. , Novikoff, P. , Faulkner, L. , Raut, S. and Gupta, S.
Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. ,
J Clin Invest
 118 , 935 , 2008
Cells
Liver endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Kondo, S. , Scheef, E. , Sheibani, N. and Sorenson, C.
PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis. ,
Am J Physiol Cell Physiol
 292 , C2070 , 2007
Cells
Endothelial kidney
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, embryonic or yolk sac
Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong.
Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation ,
J Cell Biol
 172 , 151-62 , 2006
Cells
Endothelial
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, male
Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K. and Miyasaka M
Expression profile of active genes in mouse lymph node high endothelial cells ,
Int Immunol
 11(12) , 1989 , 1999
Cells
Endothelial cells from lymph node
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, 2-4 week
Sapatino, B. , Welsh, C. , Smith, C. , Bebo, B. and Linticum, D.
Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme ,
In Vitro Cell Dev Biol
 29A , 923 , 1993
Cells
Cerebrovascular Endothelial
Enzymes
Medium
PBS
Heart
Mouse

Mouse, neonatal

Angelini, A. , Pi, X. and Xie, L.
Evaluation of Long-Chain Fatty Acid Respiration in Neonatal Mouse Cardiomyocytes using SeaHorse Instrument. ,
Star Protocols
 3 , 101392 , 2022
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.05%
CLSPA : 150 u/ml
Medium
L-15
Heart
Mouse

Mouse, adult

Zhang, Z. , Zheng, W. , He, D. , Hu, Z. , Xie, Q. , Huang, M. , Li, W. and Huang, Z.
An Improved Procedure for Isolating Adult Mouse Cardiomyocytes for Epicardial Activation Mapping. ,
J Cell Mol Med
 25 , 11257-11263 , 2021
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 35-50 u/ml
Protease : 0.005%
Medium
Tyrode solution
Heart
Mouse
Mouse
Hu, D. , Yin, C. , Mohanta, S. , Weber, C. and Habenicht, A.
Prepartaion of Single Cell Suspensions from Mouse Aorta ,
Bio Protoc
 6 , 11 , 2016
Cells
Aortic
Enzymes
Collagenase Type 1 : 400 u/ml
Collagenase : 120 u/ml
Hyaluronidase : 60 u/ml
Medium
DPBS
Heart
Mouse
Mouse, male, 12-18 week
Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R. and Chen-Izu, Y
In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. ,
PLoS ONE
 11 , e0160605 , 2016
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease XIV : 0.004%
Medium
Perfusion solution (see reference)
Heart
Mouse
Mouse, 4-6 week
Xu, J. , Lee, Y. , Ran, X. , Liao, S. , Yang, J. , Au, K. , Lai, W. , Esteban, M. and Tse, H.
Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration. ,
Stem Cells
 34 , 2693-2706 , 2016
Cells
Cardiospheres
Enzymes
Medium
PBS
Heart
Mouse

Mouse, 8-12 week

Ackers-Johnson, M. , Li, P. , Holmes, A. , O'Brien, S. , Pavlovic, D. and Foo, R.
A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. ,
Circ Res
 119 , 909-20 , 2016
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.005%
Medium
Perfusion buffer (see reference)
Heart
Mouse
Mouse, 15-30 gm
Henn, M. , Janjua, M. , Kanter, E. , Makepeace, C. , Schuessler, R. , Nichols, C. and Lawton, J.
Adenosine Triphosphate-Sensitive Potassium Channel Kir Subunits Implicated in Cardioprotection by Diazoxide. ,
J Am Heart Assoc
 4 , 2015
Cells
Ventricular myocytes
Enzymes
Medium
see reference
Heart
Mouse
Mouse, adult
Santos Nascimento, D, Mosqueira, D, Sousa, L, Teixeira, M, Filipe, M, Resende, T, Araujo, A, Valente, M, Almeida, J, Martins, J, Santos, J, Barcia, R, Cruz, P, Cruz, H. and Pinto-d-O, P
Human Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Attenuate Remodeling after Myocardial Infarction by Proangiogenic, Antiapoptotic, and Endogenous Cell-Activation Mechanisms. ,
Stem Cell Res Ther
 5 , 5 , 2014
Cells
Cardiac Progenitor
Enzymes
Medium
MEM
Heart
Mouse
Mouse, 3-4 week
Wang, H. , Chen, H. , Feng, B. , Wang, X. , He, X. , Hu, R. , Yin, M. , Wang, W. , Fu, W. and Xu, Z.
Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue. ,
BMC Biotechnol
 14 , 75 , 2014
Cells
Cardiac progenitor
Enzymes
Medium
DMEM
Heart
Mouse
Mouse
Zafiriou, M. , Noack, C. , Unsold, B. , Didie, M. , Pavlova, E. , Fischer, H. , Reichardt, H. , Bergmann, M. , El-Armouche, A. , Zimmermann, W. and Zelarayan, L.
Erythropoietin Responsive Cardiomyogenic Cells Contribute to Heart Repair Post Myocardial Infarction. ,
Stem Cells
 32 , 2480 , 2014
Cells
Cardiac progenitor
Enzymes
Medium
HBSS
Heart
Mouse
Mouse
Touchberry, C. , Green, T. , Tchikrizov, V. , Mannix, J. , Mao, T. , Carney, B. , Girgis, M. , Vincent, R. , Wetmore, L. , Dawn, B. , Bonewald, L. , Stubbs, J. and Wacker, M.
FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy. ,
Am J Physiol Endocrinol Metab
 304 , E863 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 360 u/ml
Papain : 0.4 u/ml
Medium
L-15
Heart
Mouse
Mouse, adult
Kohncke, C. Lisewski, U. , Schleussner, L. , Gaertner, C. , Reichert, S. and Roepke, T.
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes. ,
J Vis Exp
 73 , e50145 , 2013
Cells
Cardiomyocytes
Enzymes
Medium
see reference
Heart
Mouse
Mouse
Hennessey, J. , Wei, E. and Pitt, G.
Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels. ,
Circ Res
 113 , 381-8 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
MEM
Heart
Mouse
Mouse
Pratumvinit, B. , Reesukumal, K. , Janebodin, K. , Ieronimakis, N. and Reyes, M.
Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells. ,
Biomed Res Int
 2013 , 359412 , 2013
Cells
Cardiac endothelial
Enzymes
Medium
PBS
Heart
Mouse
Mouse, 2-6 month
Christel, C. , Cardona, N. , Mesirca, P. , Herrmann, S. , Hofmann, F. , Striessnig, J. , Ludwig, A. , Mangoni, M. and Lee, A.
Distinct Localization and Modulation of Cav1.2 and Cav1.3 L-type Ca2+ Channels in Mouse Sinoatrial Node. ,
J Physiol
 590 , 6327 , 2012
Cells
Sinoatrial node
Enzymes
Collagenase Type 2 : 229 u/ml
Elastase : 1.9 u/ml
Protease Type XIV : 0.9 u/ml
Medium
Tyrode's solution
Heart
Mouse
Mouse, adult
Flynn, J. , Santana, L. and Melov, S.
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes. ,
J Vis Exp
 58 , e3302 , 2011
Cells
Cardiomyocytes
Enzymes
Collagenase Type 1 : 0.1-0.4%
Protease Type XIV : 0.004-.02%
Medium
see reference
Heart
Mouse
Mouse, male, 8-25 week
Carley, A. and Kleinfeld, A.
Fatty Acid (FFA) Transport in Cardiomyocytes Revealed by Imaging Unbound FFA is Mediated by an FFA Pump Modulated by the CD36 Protein. ,
J Biol Chem
 286 , 4589-97 , 2011
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 59 u/ml
Medium
MEM
Heart
Mouse
Mouse
Butcher, M. , Herre, M. , Ley, K. and Galkina, E.
Flow Cytometry Analysis of Immune Cells Within Murine Aortas. ,
J Vis Exp
 53 , e2828 , 2011
Cells
Aortic adventitial leukocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5.6 u/ml
Medium
PBS
Heart
Mouse
Mouse, male, 6 week
Makino, A. , Suarez, J. , Wang, H. , Belke, D. , Scott, B. and Dillmann, W.
Thyroid Hormone Receptor-beta is Associated With Coronary Angiogenesis During Pathological Cardiac Hypertrophy. ,
Endocrinology
 150 , 2008 , 2009
Cells
Coronary endothelial cells
Enzymes
Medium
M199
Heart
Mouse
Mouse, adult
Zhang, Y, Kanter, E. , Laing, J. , Aprhys, C. , Johns, D. , Kardami, E. and Yamada, K.
Connexin43 Expression Levels Influence Intercellular Coupling and Cell Proliferation of Native Murine Cardiac Fibroblasts. ,
Cell Commun Adhes
 15 , 289 , 2008
Cells
Ventricular myoctes
Enzymes
Collagenase : 0.1%
Medium
M199

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.