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Tissue References Search Tool

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Epithelial
Mouse
Mouse, 8-12 week
Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.
Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
 6 , 2020
Cells
Nasal epithelium
Enzymes
Papain : 20 u/ml
Medium
EBSS
Epithelial
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J.
Parent Stem Cells Can Serve as Niches for Their Daughter Cells. ,
Nature
 523 , 597-601 , 2015
Cells
Tracheal epithelial
Enzymes
Papain : 50 u/ml
Medium
See reference
Epithelial
Mouse
Mouse, adult
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1H.4 , 2011
Cells
Cilary epithelial
Enzymes
PDS kit : per instructions
Medium
EBSS
Epithelial
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Epithelial
Mouse
Mouse
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
 446 , 77-106 , 2008
Cells
Epithelial
Enzymes
Collagenase : 0.025%
Medium
DMEM
Epithelial
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Epithelial
Mouse
Mouse, female
Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y.
Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis ,
Am J Pathol
 155 , 173-81 , 1999
Cells
Salivary gland epithelial
Enzymes
Collagenase Type 1 : 750 u/ml
Hyaluronidase : 500 u/ml
Medium
DMEM/F12
Epithelial
Mouse
Mouse, fetal 12-13 day
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Mouse
Mouse
Durban, E.
Submandibular Salivary Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:2.1 , 1995
Cells
Submandibular salivary
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Katayama, M. and Kan, M.
Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation ,
In Vitro Cell Dev Biol
 27 , 533 , 1991
Cells
Esophageal
Enzymes
Trypsin : 0.25%
Medium
PBS, CMF
Epithelial
Mouse
Mouse, mature, female, 6-8-wk-old
Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.
Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes ,
In Vitro Cell Dev Biol
 27A , 713 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
HBSS
Epithelial
Mouse
Mouse, female, 18 - 20 days
(also 20 - 22 days)
Fukamachi, H. and McLachlan, J.
Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum ,
In Vitro Cell Dev Biol
 27A , 907 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.25%
Medium
HBSS
Epithelial
Mouse
Mouse, (BALB/c), male, 3-5 months
Durban, E.M.
Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix ,
In Vitro Cell Dev Biol
 26 , 33 , 1990
Cells
Epithelial
Submandibular salivary gland
Enzymes
Collagenase Type 3 : 0.16% , 1:1 v/v
Medium
DMEM with 15 mM HEPES
Epithelial
Mouse
Mouse, female
Riser, M. , Huff, B. and Medina, D.
Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
 19 , 730 , 1983
Cells
Epithelial
Enzymes
Pepsin : 0.1% and 0.05%
Pepsin : 0.1%
Medium
HBSS with 0.2% EDTA, CMF
Epithelial
Mouse
Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant
Yang, J. , Flynn, D. , Larson, L. and Hamamoto, S.
Growth in Primary Culture of Mouse Submandibular Epithelial Cells ,
In Vitro
 18 , 435 , 1982
Cells
Epithelial
Submandibular gland
Enzymes
Medium
HBSS
Epithelial
Mouse
Mouse (BALB/cfC3H)
Yang, J. , Guzman, R. , Richards, J. and Nandi, S.
Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels ,
In Vitro
 16 , 502 , 1980
Cells
Mammary tumors
Epithelial
Enzymes
Collagenase : 1.0%
Medium
HBSS
Epithelial
Mouse
Mouse, C3H, 6-8 weeks
Lillehaug, J. , Mondal, S. and Heidelberger, C.
Establishment of Epithelial Cell Lines from Mouse Regenerating Liver ,
In Vitro
 15 , 910 , 1979
Cells
Epithelial
Enzymes
Collagenase : 0.10%
Medium
DMEM
Pancreas
Mouse
Mouse
Low, L. , Zavortink, M. , Mitchell, J. , Gan, W. , Do, O. , Schwiening, C. , Gaisano, H. and Thorn, P.
Insulin Secretion from Beta Cells in Intact Mouse Islets is Targeted Towards the Vasculature ,
Diabetologia
 57 , 1655 , 2014
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Mouse
Mouse
Koh, D. , Moody, M. and Jo, J.
Collection of Islets of Langerhans using an Equilibrium Method. ,
Biotechniques
 55 , 34-7 , 2013
Cells
Islets
Enzymes
Collagenase : 0.1-0.25%
Medium
HBSS
Pancreas
Mouse
Mouse, 8-12 week
Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E.
Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. ,
EMBO J
 32 , 2708-21 , 2013
Cells
Pancreas organoid
Enzymes
Neutral Protease : 0.012%
Collagenase : 0.012%
Medium
DMEM
Pancreas
Mouse
Mouse, embryonic
Greggio, C. , De Franceschi, F. , Figueiredo-Larsen, M. , Gobaa, S. , Ranga, A. , Semb, H. , Lutolf, M. and Grapin-Botton, A.
Artificial Three-Dimensional Niches Deconstruct Pancreas Development In Vitro. ,
Development
 140 , 4452-62 , 2013
Cells
Pancreatic progenitor
Enzymes
Neutral Protease : 0.125%
Medium
DMEM
Pancreas
Mouse
Mouse
Bertera, S. , Balamurugan, A. , Bottino, R. , He, J. and Trucco, M.
Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin. ,
J Transplant
 2012 , 856386 , 2012
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
RPMI 1640
Pancreas
Mouse
Mouse
Ding, G. , Wang, F. , Shu, J. , Tian, S. , Jiang, Y. , Zhang, D. , Wang, N. , Luo, Q. , Zhang, Y. , Jin, F. , Leung, P. , Sheng, J. and Huang, H.
Transgenerational Glucose Intolerance with Igf2/H19 Epigenetic Alterations in Mouse Islet Induced by Intrauterine Hyperglycemia. ,
Diabetes
 61 , 1133-42 , 2012
Cells
Islet
Enzymes
Medium
RPMI-1640
Pancreas
Mouse
Mouse, 10 week
Kobayashi, T. , Yamaguchi, T. , Hamanaka, S. , Kato-Itoh, M. , Yamazaki, Y. , Ibata, M. , Sato, H. , Lee, Y. , Usui, J. , Knisely, A. , Hirabayashi, M. and Nakauchi, H.
Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells. ,
Cell
 142 , 787 , 2010
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, 6-8 month
Taguchi, Y. , Tasaki, Y. , Terakado, K. , Kobayashi, K. , Machida, T. and Kobayashi, T.
Impaired Insulin Secretion from the Pancreatic Islets of Hypothyroidal Growth-Retarded Mice. ,
J Endocrinol
 206 , 195-204 , 2010
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Mouse
Mouse
Li, D. , Yuan, Y. , Tu, H. , Liang, Q. and Dai, L.
A Protocol for Islet Isolation from Mouse Pancreas. ,
Nat Protoc
 4 , 1649 , 2009
Cells
Islets
Enzymes
Collagenase : 1,000 u/ml
Medium
RPMI 1640
Pancreas
Mouse
Mouse
Carter, J. , Dula, S. , Corbin, K. , Wu, R. and Nunemaker, C.
A Practical Guide to Rodent Islet Isolation and Assessment. ,
Biol Proced Online
 11 , 3 , 2009
Cells
Islets
Enzymes
Collagenase : 0.14%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 3-4 week, 20-24 g
Huang, H. , Xie, Q. , Kang, M. , Zhang, B. , Zhang, H. , Chen, J. , Zhai, C. , Yang, D. , Jiang, B. and Wu, Y.
Labeling Transplanted Mice Islet with Polyvinylpyrrolidone Coated Superparamagnetic Iron Oxide Nanoparticles for In Vivo Detection By Magnetic Resonance Imaging. ,
Nanotechnology
 20 , 365101 , 2009
Cells
Islets
Enzymes
Medium
RPMI 1540
Pancreas
Mouse
Mouse
Szot, G. , Koudria, P. and Bluestone, J.
Murine Pancreatic Islet Isolation. ,
J Vis Exp
 7 , 255 , 2007
Cells
Islets
Enzymes
Collagenase : 0.03-0.08%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, adult
Voronina, S. , Barrow, S. , Gerasimenko, O. , Petersen, O. and Tepikin, A.
Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi. ,
J Biol Chem
 279 , 27327 , 2004
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
see reference
Pancreas
Mouse
Mouse, 11-12 week
Astrof, S. , Crowley, D. , George, E. , Fukuda, T. , Sekiguchi, K. , Hanahan, D. and Hynes, R.
Direct Test of Potential Roles of EIIIA and EIIIB Alternatively Spliced Segments of Fibronectin in Physiological and Tumor Angiogenesis. ,
Mol Cell Biol
 24 , 8662-70 , 2004
Cells
Islets
Enzymes
Medium
RPMI
Pancreas
Mouse
Mouse
Wu Yulian, Han Bing, Luo Hongyu, Roduit Raphael, Salcedo TheodoraW, Moore PaulA, Zhang Jun, Wu Jiangping.
DcR3/TR6 effectively prevents islet primary nonfunction after transplantation ,
Diabetes
 52 , 2279-86 , 2003
Cells
Islets
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.