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Miscellaneous
Mouse

Mouse

Hameed, M. , Rai, P. , Makris, M. and Weger-Lucarelli, J.
Optimized Protocol for Mouse Footpad Immune Cell Isolation for Single-cell RNA Sequencing and Flow Cytometry. ,
Star Protocols
 4 , 102409 , 2023
Cells
Leucocytes
Enzymes
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse
Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Miscellaneous
Mouse
Mouse, embryonic
He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.
Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
 9 , 53085 , 2020
Cells
Trachea
Enzymes
Elastase : 0.1%
Trypsin : 0.125%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse
Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C.
Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. ,
Nat Commun
 11 , 4061 , 2020
Cells
Adhesion fibroblasts
Enzymes
Medium
Medium 199
Miscellaneous
Mouse
Mouse, 8-12 week
Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
 , 2020
Cells
Urinary bladder
Enzymes
Collagenase Type 1 : 50 u/ml
Collagenase Type 3 : 40 u/ml
Papain : 45 u/ml
Collagenase Type 6 : 20 u/ml
Medium
Tyrode solution
Miscellaneous
Mouse
Mouse
Ishida, T. , Suzuki, S. , Lai, C. , Yamazaki, S. , Kakuta, S. , Iwakura, Y. , Nojima, M. , Takeuchi, Y. , Higashihara, M. , Nakauchi, H. and Otsu, M.
Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells. ,
Stem Cells
 35 , 989-1002 , 2017
Cells
Bone marrow stromal
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse
Huang, C. , Chen, C. and Shyr, C.
The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer. ,
Cytotherapy
 19 , 1233-1245 , 2017
Cells
Urothelial
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse
Kim, Y. , Wang, S. , Tymanskyj, S. , Ma, L. , Tao, H. and Zhang, L.
Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. ,
Sci Rep
 6 , 23799 , 2016
Cells
Cochlear
Enzymes
Papain : 20 u/ml
Trypsin : 0.05%
Medium
DMEM
Miscellaneous
Mouse
Mouse
Chavez, M. , Hu, J. , Seidel, K. , Li, C. , Jheon, A. , Naveau, A. , Horst, O. and Klein, O.
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor. ,
J Vis Exp
 , 2014
Cells
Epithelial stem
Enzymes
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse, 10 week
Futami, I. , Ishijima, M. , Kaneko, H. , Tsuji, K. , Ichikawa-Tomikawa, N. , Sadatsuki, R. , Muneta, T. , Arikawa-Hirasawa, E. , Sekiya, I. and Kaneko, K.
Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium. ,
PLoS ONE
 7 , e45517 , 2012
Cells
Synovial mesenchymal
Enzymes
Medium
DMEM
Miscellaneous
Mouse
Mouse, 26-30 g
Smith, T. , Grider, J. , Dewey, W. and Akbarali, H.
Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels. ,
PLoS ONE
 7 , e45251 , 2012
Cells
Myenteric plexus
Enzymes
Medium
Neurobasal A
Miscellaneous
Mouse
Mouse
Jan, T. , Chai, R. , Sayyid, Z. and Cheng, A.
Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry. ,
J Vis Exp
 58 , e3432 , 2011
Cells
Cochleaer
Enzymes
Trypsin : 0.125%
Medium
DMEM/F-12
Miscellaneous
Mouse
Mouse
Stock, A. , Booth, S. and Cerundolo, V.
Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans. ,
J Exp Med
 208 , 761 , 2011
Cells
Dentritic
Enzymes
Medium
DME
Miscellaneous
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse, 6-8 week
Xu, S. , De Becker, A. , Van Camp, B. , Vanderkerken, K. and Van Riet, I.
An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells. ,
J Biomed Biotechnol
 2010 , 105940 , 2010
Cells
Bone marrow mesenchymal stem
Enzymes
Medium
RPMI 1640
Miscellaneous
Mouse
Mouse, 3-6 week
Staszkiewicz, J. , Gimble, J. , Manuel, J. and Gawronska-Kozak, B.
IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential. ,
Stem Cells
 26 , 2666 , 2008
Cells
Ear mesenchymal stem
Enzymes
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse, 6-10 week
Ji, H. , Rintelen, F. , Waltzinger, C. , Meier, D, Bilancio, A. , Pearce, W. , Hirsch, E. , Wymann, M. , Ruckle, T. , Camps, M. , Vanhaesebroeck, B. , Okkenhaug, K. and Rommel, C.
Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation. ,
Blood
 110 , 2940 , 2007
Cells
Salivary gland and stomach
Enzymes
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse
Bertoncello, I. and Williams, B.
Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining. ,
Methods Mol Biol
 263 , 181 , 2004
Cells
Bone marrow
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse, 25-30 g
Minamoto Kanji, Pinsky DavidJ.
Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts ,
J Exp Med
 196 , 1321-33 , 2002
Cells
Tracheal inflammatory cells
Enzymes
Medium
RPMI 1640
Reproductive
Mouse
Mouse, 4 week
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
 11 , 55 , 2020
Cells
Trophoblast
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, adult
Lee, A, Rusch, J. , Lima, A. , Usmani, A. , Huang, N. , Lepamets, M. , Vigh-Conrad, K. , Worthington, R. and et al.
Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility. ,
Nat Commun
 10 , 4626 , 2019
Cells
Testes
Enzymes
Medium
MEM
Reproductive
Mouse
Mouse, 1-7 day
Kang, H. , Chen, L. , Lichti-Kaiser, K. , Liao, G. , Gerrish, K. , Bortner, C. , Yao, Hu. , Eddy, E. and Jetten, A.
Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis. ,
Stem Cells
 34 , 2772-2783 , 2016
Cells
Testicular
Enzymes
Medium
HBSS
Reproductive
Mouse
Mouse, adult
Martin, L. and Seandel, M.
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines. ,
J Vis Exp
 72 , e50017 , 2013
Cells
Spermatogonial stem
Enzymes
Trypsin : 0.05%
Medium
DMEM
Reproductive
Mouse
Mouse, female
Jiang, J. and Kelly, K.
Isolation of Lymphocytes from Mouse Genital Tract Mucosa. ,
J Vis Exp
 67 , e4391 , 2012
Cells
Lymphocytes
Enzymes
Collagenase : 450 u/ml
Medium
RPMI
Reproductive
Mouse
Mouse
White, Y. , Woods, D. , Takai, Y. , Ishihara, O. , Seki, H. and Tilly, J.
Oocyte Formation by Mitotically Active Germ Cells Purified from Ovaries of Reproductive-Age Aomen. ,
Nat Med
 18 , 413 , 2012
Cells
Ovarian
Enzymes
Collagenase Type 4 : 800 u/ml
Medium
HBSS
Reproductive
Mouse
Mouse
Venkataraman, L. , Lenis, A. , Couri, B. , Damaser, M. and Ramarurthi, A
Induced Regenerative Elastic Matrix Repair in LOXL 1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse ,
J Tissue Sci Eng
 3 , 3 , 2012
Cells
Vaginal smooth muscle
Enzymes
Collagenase Type 2 : 175 u/ml
Elastase : 0.025%
Medium
DMEM/F12
Reproductive
Mouse
Mouse
Yamatoya, K. , Ito, C. , Araki, M. , Furuse, R. and Toshimori, K.
One-Step Collagenase Method for Zona Pellucida Removal in Unfertilized Eggs: Easy and Gentle Method for Large-Scale Preparation. ,
Reprod Med Bio
 10 , 97-103 , 2011
Cells
Zona pellucid free eggs
Enzymes
Collagenase : 0.1%
Medium
THY-HEPES
Reproductive
Mouse
Mouse, male
Getun, I. , Torres, B. and Bois, P.
Flow Cytometry Purification of Mouse Meiotic Cells. ,
J Vis Exp
 50 , 2602 , 2011
Cells
Testis, meiotic
Enzymes
Collagenase Type 1 : 120 u/ml
Trypsin : 0.1%
Medium
Gey's BSS
Reproductive
Mouse
Mouse
Chen, L. , Belton, R. and Nowak, R.
Basigin-Mediated Gene Expression Changes in Mouse Uterine Stromal Cells During Implantation. ,
Endocrinology
 150 , 966 , 2009
Cells
Uterine stomal
Enzymes
Medium
DMEM/F12
Reproductive
Mouse
Mouse, embryonic
Gekas, C. , Rhodes, K. and Mikkola, H.
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta. ,
J Vis Exp
 16 , e742 , 2008
Cells
Hematopoietic stem cells
Enzymes
Collagenase : 0.1%
Medium
PBS
Reproductive
Mouse
Mouse
Breault, D. , Min, I. , Carlone, D. , Farilla, L. , Ambruzs, D. , Henderson, D. , Algra, S. , Montgomery, R. , Wagers, A. and Hole, N.
Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells. ,
Proc Natl Acad Sci U S A
 105 , 10420 , 2008
Cells
Germ cells
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
HBSS
Reproductive
Mouse
Mouse, adult, neonatal and fetal
O'Shaughnessy PJ, Fleming LM, Jackson G, Hochgeschwender U, Reed P, Baker PJ.
Adrenocorticotropic hormone directly stimulates testosterone production by the fetal and neonatal mouse testis ,
Endocrinology
 144 , 3279-84 , 2003
Cells
Testicular cells
Enzymes
Medium
DMEM/F-12
Reproductive
Mouse
Mouse, 6 day
Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta.
Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse ,
Biol Reprod
 69 , 985-94 , 2003
Cells
Sertoli
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, male, 3.5 months old
Faldikova, L. , Diblikova, I. , Canderle, J. , Zraly, Z. , Veznik, Z. and Sulcova, A.
Effects of Nutrition, Social Factors and Chronic Stress on the Mouse Leydig Cell Testosterone Production ,
Vet Med
 46 (6) , 160 , 2001
Cells
Leydig
Enzymes
Collagenase : 0.06%
Medium
Medium E 199
Reproductive
Mouse
Mouse, male, 12-14-week-old
Lin, Q. , Sirotkin, A. and Skoultchi, A.
Normal Spermatogenesis in Mice Lacking the Testis-Specific Linker Histone H1t ,
Mol Cell Biol
 20 (6) , 2122 , 2000
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
DMEM
Reproductive
Mouse
Mouse, 6-12 day
Eppig JJ, O'Brien MJ.
Development in vitro of mouse oocytes from primordial follicles ,
Biol Reprod
 54 , 197-207 , 1996
Cells
Oocyte-granulosa
Enzymes
Medium
Waymouth

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.