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Tissue References Search Tool

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Intestine
Mouse

Mouse

Kumar, S. , Suman, S. , Li, H. , Angdisen, J. , Datta, K. and Fornace, A.
High-linear Energy Transfer Radiation Disrupts Natural Killer Cell Surveillance of Senescent Intestinal Cells in the Mouse Intestine. , Mol Biomed Vol. 7 , 2026
Cells
Organoids
Enzymes
Medium
HBSS
Intestine
Mouse

Mouse, adult

Kumar, S. , Suman, S. , Angdisen, J. , Moon, B. , Kallakury, B. , Datta, K. and Fornace, A.
Effects of High-Linear-Energy-Transfer Heavy Ion Radiation on Intestinal Stem Cells: Implications for Gut Health and Tumorigenesis. ,
Cancers
 16 , 2024
Cells
Intestinal stem
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse
Emgard, J. , Kammoun, H. , Garcia-Cassani, B. , Chesne, J. , Parigi, S. , Jacob, J. , Cheng, H, Evren, E. , Das, S. , Czarnewski, P. , Sleiers, N. Melo-Gonzalez, F. , Kvedaraite, E. Svensson, M. and Scandella, E.
Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. ,
Immunity
 48 , 120-132.e8 , 2018
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse, embryonic
Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J. and Ming L
Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase. ,
Braz J Med Biol Res
 50 , e5831 , 2017
Cells
Crypt
Enzymes
Collagenase Type 1 : 200 u/ml
Hyaluronidase : 100u/ml
Medium
DMEM
Intestine
Mouse
Mouse
O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
 6 , 2016
Cells
Intestinal organoids
Enzymes
Medium
Basal Medium
Intestine
Mouse
Mouse
Seo, S. , Kuffa, P. , Kitamoto, S. , Nagao-Kitamoto, H. , Rousseau, J. , Kim, Y. , Nunez, G. and Kamada, N.
Intestinal Macrophages Arising from CCR2(+) Monocytes Control Pathogen Infection by Activating Innate Lymphoid Cells. ,
Nat Commun
 6 , 8010 , 2015
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse
Beura, L. , Anderson, K. , Schenkel, J. , Locquiao, J. , Fraser, K. , Vezys, V. , Pepper, M. and Masopust, D.
Lymphocytic Choriomeningitis Virus Persistence Promotes Effector-Like Memory Differentiation and Enhances Mucosal T cell Distribution. ,
J Leukoc Biol
 97 , 217 , 2015
Cells
Lymphocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
RPMI-1640
Intestine
Mouse
Zhang, Y. and Hu, W.
Mouse Enteric Neuronal Cell Culture. ,
Methods Mol Biol
 1078 , 55-63 , 2013
Cells
Enteric neurons
Enzymes
Trypsin : 0.05%
Deoxyribonuclease I : 400 u/ml
Medium
DMEM/F12
Intestine
Mouse
Mouse, P4
Hotta, R. , Stamp, L. , Foong, J. , McConnell, S. , Bergner, A. , Anderson, R. , Enomoto, H. , Newgreen, D. , Obermayr, F. , Furness, J. and Young, H.
Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon. ,
J Clin Invest
 123 , 1182 , 2013
Cells
Enteric neural crest progenitors
Enzymes
Medium
DMEM/F12
Intestine
Mouse
Mouse
Xue, X. and Shah, Y.
In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
 , e50210 , 2013
Cells
Colon tumor organoids
Enzymes
Medium
DMEM
Intestine
Mouse
Mouse
Geem, D. , Medina-Contreras, O. , Kim, W. , Huang, C. and Denning T.
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine. ,
J Vis Exp
 63 , e4040 , 2012
Cells
Dendritic, macrophages
Enzymes
Collagenase : 0.15%
Medium
HBSS
Intestine
Mouse
Mouse, 2-3 month
Becker, L. , Kulkarni, S. , Tiwari, G. , Micci, M. and Pasricha, P.
Divergent Fate and Origin of Neurosphere-Like Bodies from Different Layers of the Gut. ,
Am J Physiol Gastrointest Liver Physiol
 302 , G958-65 , 2012
Cells
Enteric neural stem cells
Enzymes
Medium
M199
Intestine
Mouse
Mouse
Barthel, E. , Speer, A. , Levin, D. , Sala, F. , Hou, X. , Torashima, Y. , Wigfall, C. and Grikscheit, T.
Tissue Engineering of the Intestine in a Murine Model. ,
J Vis Exp
 70 , e4279 , 2012
Cells
Intestinal organoids
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Intestine
Mouse
Mouse
Ruiz, V. , Sachdev, M. , Zhang, S. , Wen, S. and Moss, S.
Isolating, Immunophenotyping and Ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes During Murine Helicobacter Pylori Infection. ,
J Immunol Methods
 384 , 157-63 , 2012
Cells
Lyphocytes
Enzymes
Medium
RPMI 1640
Intestine
Mouse
Mouse, neonatal and adult
Joseph, N. , He, S. , Quintana, E. , Kim, Y. , Nunez, G. and Morrison, S.
Enteric Glia are Multipotent in Culture but Primarily Form Glia in the Adult Rodent Gut. ,
J Clin Invest
 121 , 3398 , 2011
Cells
Myenteric plexus
Enzymes
Collagenase Type 4 : 0.025-0.1%
Papain : 10 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
HBSS
Intestine
Mouse
Mouse
Ito, Y. , Kanai, T. , Totsuka, T. , Okamoto, R. , Tsuchiya, K. , Nemoto, Y. , Yoshioka, A. , Tomita, T. , Nagaishi, T. , Sakamoto, N. , Sakanishi, T. , Okumura, K. , Yagita, H. and Watanabe, M.
Blockade of NKG2D Signaling Prevents the Development of Murine CD4+ T Cell-Mediated Colitis. ,
Am J Physiol Gastrointest Liver Physiol
 294 , G199 , 2008
Cells
Lamina propria lymphocytes
Enzymes
Medium
RPMI 1640
Intestine
Mouse
Mouse
Forbes, E. , Groschwitz, K. , Abonia, J.P. , Brandt, E. , Cohen, E. , Blanchard, C. , Ahrens, R. , Seidu, L. , McKenzie, A. , Strait, R. , Finkelman, F. , Foster, P. , Matthaei, K. , Rothenberg, M. and Hogan, S.
IL-9- and Mast Cell-Mediated Intestinal Permeability Predisposes to Oral Antigen Hypersensitivity. ,
J Exp Med
 205 , 897 , 2008
Cells
Intestinal mononuclear
Enzymes
Medium
RPMI 1640
Intestine
Mouse
Mouse, Mastomys natalensis
Kidd, M. , Modlin, I. , Eick, G. and Champaneria, M.
Isolation, Functional Characterization, and Transcriptome of Mastomys Ileal Enterochromaffin Cells. ,
Am J Physiol Gastrointest Liver Physiol
 291 , G778-91 , 2006
Cells
Enterochromaffin cells
Enzymes
Collagenase : 0.025%
Pronase E : 0.07%
Medium
HBSS
Intestine
Mouse
Mouse, 7 week
Goto Kazunori, Matsuoka Satoshi, Noma Akinori.
Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine ,
J Physiol
 559 , 411-22 , 2004
Cells
Interstitial cells
Enzymes
Collagenase : 0.04%
Trypsin : 0.02%
Medium
see reference
Intestine
Mouse
Mouse, 9-13 day
Ordag Tamas, Redelman Doug, Miller Lisa J, Horvath Viktor J, Zhong Qiao, Almeida-Porada Graca, Zanjani Esmail D, Horowitz Burton, Sanders Kenton M.
Purification of interstitial cells of Cajal by fluorescence-activated cell sorting ,
Am J Physiol Cell Physiol
 286 , C448-56 , 2004
Cells
Interstitial cells of Cajal
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse, 0-15 day
Lee Young Mee, Kim Byung Joo, Kim Hyun Jin, Yang Dong Ki, Zhu Mei Hong, Lee Kyu Pil, So Insuk, Kim Ki Whan.
TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach ,
Am J Physiol/Gastro
 284 , G604-16 , 2003
Cells
Interstitial cells of Cajal
Enzymes
Medium
Hanks
Intestine
Mouse
Mouse
Wu Y, Wang X, Csencsits KL, Haddad A, Walters N, Pascual DW.
M cell-targeted DNA vaccination ,
Proc Natl Acad Sci U S A
 98 , 9318-23 , 2001
Cells
Lamina propria lymphocytes
Enzymes
Collagenase Type 4 : 300 u/ml
Medium
PBS
Intestine
Mouse
Mouse, F14
Sakagami Y, Inaguma Y, Sakakura T, Nishizuka Y.
Intestine-like remodeling of adult mouse glandular stomach by implanting of fetal intestinal mesenchyme ,
Cancer Res
 44 , 5845-9 , 1984
Cells
Intestinal mesenchymal
Enzymes
Medium
HBSS
Miscellaneous
Mouse

Mouse

Hameed, M. , Rai, P. , Makris, M. and Weger-Lucarelli, J.
Optimized Protocol for Mouse Footpad Immune Cell Isolation for Single-cell RNA Sequencing and Flow Cytometry. ,
Star Protocols
 4 , 102409 , 2023
Cells
Leucocytes
Enzymes
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse
Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Miscellaneous
Mouse
Mouse, embryonic
He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.
Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
 9 , 53085 , 2020
Cells
Trachea
Enzymes
Elastase : 0.1%
Trypsin : 0.125%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse
Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C.
Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. ,
Nat Commun
 11 , 4061 , 2020
Cells
Adhesion fibroblasts
Enzymes
Medium
Medium 199
Miscellaneous
Mouse
Mouse, 8-12 week
Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
 , 2020
Cells
Urinary bladder
Enzymes
Collagenase Type 1 : 50 u/ml
Collagenase Type 3 : 40 u/ml
Papain : 45 u/ml
Collagenase Type 6 : 20 u/ml
Medium
Tyrode solution
Miscellaneous
Mouse
Mouse
Ishida, T. , Suzuki, S. , Lai, C. , Yamazaki, S. , Kakuta, S. , Iwakura, Y. , Nojima, M. , Takeuchi, Y. , Higashihara, M. , Nakauchi, H. and Otsu, M.
Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells. ,
Stem Cells
 35 , 989-1002 , 2017
Cells
Bone marrow stromal
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse
Huang, C. , Chen, C. and Shyr, C.
The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer. ,
Cytotherapy
 19 , 1233-1245 , 2017
Cells
Urothelial
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse
Kim, Y. , Wang, S. , Tymanskyj, S. , Ma, L. , Tao, H. and Zhang, L.
Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. ,
Sci Rep
 6 , 23799 , 2016
Cells
Cochlear
Enzymes
Papain : 20 u/ml
Trypsin : 0.05%
Medium
DMEM
Miscellaneous
Mouse
Mouse
Chavez, M. , Hu, J. , Seidel, K. , Li, C. , Jheon, A. , Naveau, A. , Horst, O. and Klein, O.
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor. ,
J Vis Exp
 , 2014
Cells
Epithelial stem
Enzymes
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse, 10 week
Futami, I. , Ishijima, M. , Kaneko, H. , Tsuji, K. , Ichikawa-Tomikawa, N. , Sadatsuki, R. , Muneta, T. , Arikawa-Hirasawa, E. , Sekiya, I. and Kaneko, K.
Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium. ,
PLoS ONE
 7 , e45517 , 2012
Cells
Synovial mesenchymal
Enzymes
Medium
DMEM
Miscellaneous
Mouse
Mouse, 26-30 g
Smith, T. , Grider, J. , Dewey, W. and Akbarali, H.
Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels. ,
PLoS ONE
 7 , e45251 , 2012
Cells
Myenteric plexus
Enzymes
Medium
Neurobasal A
Miscellaneous
Mouse
Mouse
Jan, T. , Chai, R. , Sayyid, Z. and Cheng, A.
Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry. ,
J Vis Exp
 58 , e3432 , 2011
Cells
Cochleaer
Enzymes
Trypsin : 0.125%
Medium
DMEM/F-12
Miscellaneous
Mouse
Mouse
Stock, A. , Booth, S. and Cerundolo, V.
Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans. ,
J Exp Med
 208 , 761 , 2011
Cells
Dentritic
Enzymes
Medium
DME

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.