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Tissue References Search Tool

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Miscellaneous
Mouse

Mouse

Hameed, M. , Rai, P. , Makris, M. and Weger-Lucarelli, J.
Optimized Protocol for Mouse Footpad Immune Cell Isolation for Single-cell RNA Sequencing and Flow Cytometry. ,
Star Protocols
 4 , 102409 , 2023
Cells
Leucocytes
Enzymes
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse
Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Miscellaneous
Mouse
Mouse, embryonic
He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.
Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
 9 , 53085 , 2020
Cells
Trachea
Enzymes
Elastase : 0.1%
Trypsin : 0.125%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse
Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C.
Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. ,
Nat Commun
 11 , 4061 , 2020
Cells
Adhesion fibroblasts
Enzymes
Medium
Medium 199
Miscellaneous
Mouse
Mouse, 8-12 week
Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
 , 2020
Cells
Urinary bladder
Enzymes
Collagenase Type 1 : 50 u/ml
Collagenase Type 3 : 40 u/ml
Papain : 45 u/ml
Collagenase Type 6 : 20 u/ml
Medium
Tyrode solution
Miscellaneous
Mouse
Mouse
Ishida, T. , Suzuki, S. , Lai, C. , Yamazaki, S. , Kakuta, S. , Iwakura, Y. , Nojima, M. , Takeuchi, Y. , Higashihara, M. , Nakauchi, H. and Otsu, M.
Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells. ,
Stem Cells
 35 , 989-1002 , 2017
Cells
Bone marrow stromal
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse
Huang, C. , Chen, C. and Shyr, C.
The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer. ,
Cytotherapy
 19 , 1233-1245 , 2017
Cells
Urothelial
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse
Kim, Y. , Wang, S. , Tymanskyj, S. , Ma, L. , Tao, H. and Zhang, L.
Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. ,
Sci Rep
 6 , 23799 , 2016
Cells
Cochlear
Enzymes
Papain : 20 u/ml
Trypsin : 0.05%
Medium
DMEM
Miscellaneous
Mouse
Mouse
Chavez, M. , Hu, J. , Seidel, K. , Li, C. , Jheon, A. , Naveau, A. , Horst, O. and Klein, O.
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor. ,
J Vis Exp
 , 2014
Cells
Epithelial stem
Enzymes
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse, 10 week
Futami, I. , Ishijima, M. , Kaneko, H. , Tsuji, K. , Ichikawa-Tomikawa, N. , Sadatsuki, R. , Muneta, T. , Arikawa-Hirasawa, E. , Sekiya, I. and Kaneko, K.
Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium. ,
PLoS ONE
 7 , e45517 , 2012
Cells
Synovial mesenchymal
Enzymes
Medium
DMEM
Miscellaneous
Mouse
Mouse, 26-30 g
Smith, T. , Grider, J. , Dewey, W. and Akbarali, H.
Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels. ,
PLoS ONE
 7 , e45251 , 2012
Cells
Myenteric plexus
Enzymes
Medium
Neurobasal A
Miscellaneous
Mouse
Mouse
Jan, T. , Chai, R. , Sayyid, Z. and Cheng, A.
Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry. ,
J Vis Exp
 58 , e3432 , 2011
Cells
Cochleaer
Enzymes
Trypsin : 0.125%
Medium
DMEM/F-12
Miscellaneous
Mouse
Mouse
Stock, A. , Booth, S. and Cerundolo, V.
Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans. ,
J Exp Med
 208 , 761 , 2011
Cells
Dentritic
Enzymes
Medium
DME
Miscellaneous
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse, 6-8 week
Xu, S. , De Becker, A. , Van Camp, B. , Vanderkerken, K. and Van Riet, I.
An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells. ,
J Biomed Biotechnol
 2010 , 105940 , 2010
Cells
Bone marrow mesenchymal stem
Enzymes
Medium
RPMI 1640
Miscellaneous
Mouse
Mouse, 3-6 week
Staszkiewicz, J. , Gimble, J. , Manuel, J. and Gawronska-Kozak, B.
IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential. ,
Stem Cells
 26 , 2666 , 2008
Cells
Ear mesenchymal stem
Enzymes
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse, 6-10 week
Ji, H. , Rintelen, F. , Waltzinger, C. , Meier, D, Bilancio, A. , Pearce, W. , Hirsch, E. , Wymann, M. , Ruckle, T. , Camps, M. , Vanhaesebroeck, B. , Okkenhaug, K. and Rommel, C.
Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation. ,
Blood
 110 , 2940 , 2007
Cells
Salivary gland and stomach
Enzymes
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse
Bertoncello, I. and Williams, B.
Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining. ,
Methods Mol Biol
 263 , 181 , 2004
Cells
Bone marrow
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse, 25-30 g
Minamoto Kanji, Pinsky DavidJ.
Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts ,
J Exp Med
 196 , 1321-33 , 2002
Cells
Tracheal inflammatory cells
Enzymes
Medium
RPMI 1640
Organoids
Mouse

Mouse

Fujimura, T. , Enomoto, Y. , Katsura, H. , Ogawa, T. , Baba, S. , Ogata, A. , Yamaoka, A. , Shiroguchi, K. and Morimoto, M.
Identifying a Lung Stem Cell Subpopulation by Combining Single-Cell Morphometrics, Organoid Culture, and Transcriptomics. ,
Stem Cells
 41 , 809-820 , 2023
Cells
Lung
Enzymes
Collagenase Type 1 : 450 u/ml
Elastase : 1 u/ml
Neutral Protease : 5 u/ml
Medium
DMEM/F12
Organoids
Mouse

Mouse

Klemke, L. , Blume, J. , Oliveira, T. and Schulz-Heddergott, R.
Preparation and Cultivation of Colonic and Small Intestinal Murine Organoids Including Analysis of Gene Expression and Organoid Viability. ,
Bio Protoc
 12 , e4298 , 2022
Cells
Colonic tumor organoids
Enzymes
Medium
PBS
Organoids
Mouse

Mouse

Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Organoids
Mouse

Mouse

Charifou, E. , Sumbal, J. , Koledova, Z. , Li, H. and Chiche, A.
A Robust Mammary Organoid System to Model Lactation and Involution-like Processes. ,
Bio Protoc
 11 , e3996 , 2021
Cells
Mammary organoids
Enzymes
Trypsin : 0.2%
Medium
DMEM/F12
Organoids
Mouse

Mouse, adult

Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al
Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
 175 , 1972 , 2018
Cells
Organoids
Enzymes
Collagenase Type 4 : 200-300 u/ml
Medium
DMEM
Organoids
Mouse

Mouse, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
Organoids
Mouse

Mouse

O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
 6 , 2016
Cells
Intestinal organoids
Enzymes
Medium
Basal Medium
Organoids
Mouse

Mouse, 8-12 week

Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E.
Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. ,
EMBO J
 32 , 2708-21 , 2013
Cells
Pancreas organoid
Enzymes
Neutral Protease : 0.012%
Collagenase : 0.012%
Medium
DMEM
Organoids
Mouse

Mouse

Xue, X. and Shah, Y.
In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
 , e50210 , 2013
Cells
Colon tumor organoids
Enzymes
Medium
DMEM
Organoids
Mouse

Mouse

Barthel, E. , Speer, A. , Levin, D. , Sala, F. , Hou, X. , Torashima, Y. , Wigfall, C. and Grikscheit, T.
Tissue Engineering of the Intestine in a Murine Model. ,
J Vis Exp
 70 , e4279 , 2012
Cells
Intestinal organoids
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.