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Tissue References Search Tool

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Adrenal
Rat
Rat
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Rat
Rat
Gilabert, J, Montalvo, G. and Artalejo A.
Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays ,
Nat Protoc
 , 294 , 2006
Cells
Chromaffin
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD
Gilabert, J.
Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture , Cell Biology of the Chromaffin Cell , Borges, R. and Gandia, L. , Instituto Teofilo Hernando , 2004
Cells
Chromaffin cells
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD, male
Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel.
An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells ,
Am J Physiol Regul Integr Comp Physiol
 284 , R1631-5 , 2003
Cells
Zona fasciculata/reticularis
Enzymes
Collagenase : 0.4%
Medium
Krebs-HEPES
Adrenal
Rat
Rat, Wistar, newborn
Zhang L, Castell A, Avila E, Drucker-Colín R, Escobar A.
Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons ,
J Neuropathol Exp Neurol
 59 , 170-4 , 2000
Cells
Chromaffin cells
Enzymes
Medium
DMEM
Adrenal
Rat
Rat, SD, male
Sayed, S. , Whitehouse, B. and Jones, P.
Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis ,
J Endocrinol
 154 , 449 , 1997
Cells
ZG
ZFR
Enzymes
Medium
Kreb's
Adrenal
Rat
Rat, Fischer, male, 10-16 weeks
Roskelley, C.D. and Auersperg, N.
Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture ,
In Vitro Cell Dev Biol
 26 , 493 , 1990
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, SD, 2-4 day old
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Adrenal
Rat
Rat, male, 120-160 g,
Rat, SD, male, 400-450 g
Ng, T. and Liu, W.
Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis ,
In Vitro Cell Dev Biol
 26 , 24 , 1990
Cells
Leydig
Adrenal
Enzymes
Collagenase Type 2 : 0.03% (adrenal)
Medium
Krebs Ringer bicarbonate buffer
Adrenal
Rat
Rat, Long-Evans, female, 150-200 g
Payet, N. , Deziel, Y. and Lehoux, J.-G.
Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture ,
J Steroid Biochem
 20 , 449 , 1984
Cells
Glomerulosa
Enzymes
Collagenase : 0.2%
Medium
MEM-d-Val
Adrenal
Rat
Rat, Fischer, male, 1-10 months
Leonard, R.K. , Auersperg, N. and Parkes, C.O.
Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells ,
In Vitro
 19 , 46 , 1983
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, SD, male, 400-450 g
Li, C.H. , Ng, T.B. and Cheng, C.H.K.
Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells ,
Int J Pept Protein Res
 19 , 361 , 1982
Cells
Decapular
Capsular
Glomerulosa
Enzymes
Medium
Medium 199
Adrenal
Rat
Rat, Hanover-Wistar, 2nd postnatal week (also guinea-pig, cattle)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Rat
Rat , SD, female, 200 g
Braley, L. , Williams, G. and Bradwin, G.
The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells ,
Endocrinology
 106 (1) , 50 , 1980
Cells
Glomerulosa
Enzymes
Medium
Kreb's
Adrenal
Rat
Rat
Englert, D.F.
An Optical Study of Isolated Rat Adrenal Chromaffin Cells ,
Exp Cell Res
 125 , 369 , 1980
Cells
Chromaffin
Enzymes
Trypsin : 0.10%
Medium
Ham's F-12 w/HEPES
Adrenal
Rat
Rat
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Adrenal
Rat
Rat, Wistar-Hanover, 7-12 day
Unsicker, K. , Krisch, B. , Otten, U. and Thoenen, H.
Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids ,
Proc Natl Acad Sci U S A
 75 (7) , 3498 , 1978
Cells
Medullary
Enzymes
Trypsin : 0.125%
Medium
HBSS
Adrenal
Rat
Rat, SD, male
Barofsky, A. , Feinstein, M. and Halkerston, I.
Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands ,
Exp Cell Res
 79 , 263 , 1973
Cells
Cortical
Enzymes
Trypsin : 0.25%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Rat
Rat, Holtzman, male, 180-250 g
Kloppenborg, P. , Island, D. , Liddle, G. , Michelakis, A. and Nicholson, W.
A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism ,
Endocrinology
 82 , 1053 , 1968
Cells
Adrenal
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Neural
Rat
Rat, 2-3 week
Lin, Y. and Chen, J.
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release. ,
J Vis Exp
 , 2018
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Rat
Rat, SD, 3 month
Andersen, N. and Monje, P.
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers. ,
Methods Mol Biol
 1739 , 49-66 , 2018
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, SD, day 2
Maurel, P.
Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies. ,
Methods Mol Biol
 1739 , 17-37 , 2018
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Rat
Rat, Wistar, 3 day
Fangmann, L. , Teller, S. , Stupakov, P. , Friess, H. , Ceyhan, G. and Demir, I.
3D Cancer Migration Assay with Schwann Cells. ,
Methods Mol Biol
 1739 , 317-325 , 2018
Cells
Dorsal root ganglia neurons
Enzymes
Medium
MEM
Neural
Rat
Rat, adult
George, D. , Ahrens, P. and Lambert, S.
Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells. ,
Glia
 , 2018
Cells
Schwann
Enzymes
Stemxyme 1 : 0.05%
Medium
DMEM
Neural
Rat
Rat, SD, p7
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, E17
Hayakawa, K. , Esposito, E. , Wang, X. , Terasaki, Y. , Liu, Y. , Xing, C. , Ji. X. and Lo, E.
Transfer of Mitochondria from Astrocytes to Neurons after Stroke. ,
Nature
 535 , 551-5 , 2016
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Rat
Rat, SD
Andersen, N. , Srinivas, S. , Pinero, G. and Monje, P.
A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves. ,
Sci Rep
 6 , 31781 , 2016
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, SD
Schuh, C. , Hercher, D. , Stainer, M. , Hopf, R. , Teuschl, A. , Schmidhammer, R. and Redl, H.
Extracorporeal Shockwave Treatment: A Novel Tool to Improve Schwann Cell Isolation and Culture. ,
Cytotherapy
 18 , 760-70 , 2016
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, 6-8 week
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Neural
Rat
Rat, embryonic
Oyanagi, K. , Tashiro, T. and Negishi, T.
Cell-Type-Specific and Differentiation-Status-Dependent Variations in Cytotoxicity of Tributyltin in Cultured Rat Cerebral Neurons and Astrocytes. ,
J Toxicol Sci
 40 , 459-68 , 2015
Cells
Cerebral neurons
Enzymes
Papain : 2 u/ml
Medium
DMEM-PBS
Neural
Rat
Rat, SD, 6-9 week
Murayama, C. , Watanabe, S. and Nakamura, M. and, Norimoto, H.
Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons. ,
Molecules
 20 , 14959-69 , 2015
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Rat
Rat, embryonic and neonatal
Rheaume, C. , Cai, B. , Wang, J. , Fernandez-Salas, E. , Aoki, K. , Francis, J. and Broide, R.
A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25. ,
Toxins
 7 , 2354-70 , 2015
Cells
Cortical neurons, DRG
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Neural
Rat
Rat
Yu, R. , Seymour, V. , Berecki, G. , Jia, X. , Akca, M. , Adams, D. , Kaas, Q. and Craik, D.
Less is More: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic α-Conotoxin Vc1.1. ,
Sci Rep
 5 , 13264 , 2015
Cells
Dorsal root ganglion neurons
Enzymes
Medium
HBSS
Neural
Rat
Rat, SD
Wei, X. , Yan, J. , Tillu, D. , Asiedu, M. , Weinstein, N. , Melemedjian, O. , Price, T. and Dussor, G.
Meningeal Norepinephrine Produces Headache Behaviors in Rats via Actions Both on Dural Afferents and Fibroblasts. ,
Cephalalgia
 35 , 1054-64 , 2015
Cells
Trigeminal ganglion
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Neural
Rat
Rat, neonatal
Stettner, M. , Lohmann, B. , Wolffram, K. , Weinberger, J. , Dehmel, T. , Hartung, H. , Mausberg, A. and Kieseier, B.
Interleukin-17 Impedes Schwann Cell-Mediated Myelination. ,
J Neuroinflammation
 11 , 63 , 2014
Cells
Schwann cells
Enzymes
Collagenase : 0.05-0.1%
Trypsin : 0.125-0.25%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.