Tissue References Search Tool

Muscle

Rat

Rat, Wistar Kyoto, 10-15 weeks

McGuire, P. , Walker-Caprioglio, H. , Little, S. and McGuffee, L.

Isolation and Culture of Rat Superior Mesenteric Artery Smooth Muscle Cells ,
In Vitro Cell Dev Biol
29 , 135 , 1993

Cells

Smooth muscle, mesenteric artery

Enzymes

Trypsin : 0.05%

Medium

MEM

Muscle

Rat

Rat
(also rabbit, guinea-pig)

Hu, S. and Kim, H.

Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
6 , A383 , 1992

Cells

Smooth muscle

Enzymes

Trypsin : 0.1%

Medium

Potassium buffer solution

Muscle

Rat

Rat, SD, 19 days

Loch-Caruso, R. , Pahl, M. and Juberg, D.

Rat Myometrial Smooth Muscle Cells Show High Levels of Gap Junctional Communication Under a Variety of Culture Conditions ,
In Vitro Cell Dev Biol
28 , 97 , 1992

Cells

Smooth muscle, myometrial

Enzymes

Trypsin : 150 µg/ml

Medium

HBSS or PSS, CMF

Muscle

Rat

Rat, SHRs and WKY, male, 10 - 14 weeks

Bolzon, B. and Cheung, D.

Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
14 , 137 , 1989

Cells

Smooth muscle, tail arteries

Enzymes

Papain : 0.1%

Medium

HEPES buffer

(see reference)

Muscle

Rat

Rat, 1-3 day

Barone, L. , Wolfe, L. , Faris, B. and Franzblau, C.

Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat ,
Biochemistry
27 , 3175 , 1988

Cells

Smooth muscle, aortic

Enzymes

Elastase : 0.0125%

Medium

DMEM

Muscle

Rat

Bean, B. , Sturek, M. , Puga, A. and Hermsmeyer, K.

Calcium Channels in Muscle Cells Isolated From Rat Mesenteric Arteries: Modulation by Dihydropyridine Drugs ,
Circ Res
59 , 229 , 1986

Cells

Muscle, mesenteric arteries

Enzymes

Trypsin : 0.05%

Medium

HEPES KG solution

(see reference)

Muscle

Rat

Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders

Gordon, D. , Mohai, L. and Schwartz, S.

Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells ,
Circ Res
59 , 633 , 1986

Cells

Endothelial, aortic

Enzymes

Elastase : 0.05%

Medium

Waymouth's culture medium

Muscle

Rat

Rat, male, 150-250 g

Cole, O. , Fan, T. and Lewis, G.

Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta ,
Cell Biol Int Rep
10 (6) , 399 , 1986

Cells

Endothelial, aortic

Medium

RPMI 1640

Muscle

Rat

Rat, SD, male, 200 - 250 g

Brock, T. , Alexander, R. , Ekstein, L. , Atkinson, W. and Gimbrone, M.

Angiotensin Increases Cytosolic Free Calcium in Cultured Vascular Smooth Muscle Cells ,
Hypertension
7 , 105 , 1985

Cells

Smooth muscle, thoracic aorta

Enzymes

Trypsin : 0.0375%

Medium

Eagle's MEM with calcium

Muscle

Rat

Rat, Wistar, female, 10 weeks

Boulanger-Saunier, C. , Kattenburg, D. and Stoclet, J.

Cyclic AMP-dependent Phosphorylation of a 16kDa Protein in a Plasma Membrane-enriched Fraction of Rat Aortic Myocytes ,
F.E.B.S. Lett.
193 , 283 , 1985

Cells

Myocytes

Enzymes

Collagenase : 0.1%

Medium

HBSS

Muscle

Rat

Rat, SD, 225-250g

Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.

Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
92 , 289 , 1982

Cells

Mesenteric artery smooth muscle cells

Enzymes

Elastase : .0125%

Soybean Trypsin Inhibitor : 0.025%

Collagenase Type 1 : 0.1%

Medium

HBSS

Muscle

Rat

Rat, 3-4 day

Kasten, F.

Rat Myocardial Cells In Vitro: Mitosis and Differentiated Properties ,
In Vitro
8 , 128 , 1972

Cells

Myocardial

Enzymes

Trypsin NF 1:250 : 0.125%

Medium

HBSS CMF

Muscle

Rat

Rat, 200 g

Kono, T.

Roles of Collagenases and Other Proteolytic Enzymes in the Dispersal of Animal Tissues ,
Biochim Biophys Acta
178 , 397 , 1969

Cells

Muscle

Enzymes

Trypsin : 0.05%

Medium

Kreb's Henseleit bicarbonate buffer

Muscle

Rat

Rat, Wistar, 3-10 day

Harary, I. and Farley, B.

In Vitro Studies on Single Beating Rat Heart Cells ,
Exp Cell Res
29 , 451 , 1963

Cells

Heart

Enzymes

Trypsin NF 1:250 : 250: 0.1%

Medium

Saline A

(See reference)

Tumor

Canine

Canine, adult

Petrouškova, P. , Hudáková, N. , Almášiová, V. , Valenčáková, A. , Horňáková, L. , Huniadi, M. and Čížková, D.

Establishment of Primary Cell Cultures from Canine Mammary Gland Malignant Tumours: A Preliminary Study. , J Vet Res Vol. 69 , 159-168 , 2025

Cells

Mammary gland tumor

Enzymes

Collagenase Type 4 : 0.05%

Medium

EBSS

Tumor

Hamster

Hamster, 90-100 g

Gonzalez, A. , Oberley, T. , Schultz, J. , Ostrom, J. and Li, J.

In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney ,
In Vitro Cell Dev Biol
29A , 562 , 1993

Cells

Tumor

Enzymes

Hyaluronidase : 0.1%

Medium

Waymouth's MB

Tumor

Hamster

Hamster, 6 week old

Min, B. , Kim, K. , Cherrick, H. and Park, N.

Three Cell Lines from Hamster Buccal Pouch Tumors Induced by Topical 7,12-Dimethylbenz(a)Anthracene, Alone or in Conjunction with Herpes Simplex Virus Inoculation ,
In Vitro Cell Dev Biol
27A , 128 , 1991

Cells

Buccal pouch

Enzymes

Neutral Protease : 0.24%

Medium

CMF HBSS

Tumor

Human

Song, H. , Weinstein, H. , Allegakoen, P. , Wadsworth, M. , Xie, J. , Yang, H. , Castro, E. , Lu, K. , Stohr, B. , Feng, F. , Carroll, P. , Wang, B. , Cooperberg, M. , Shalek, A. and Huang, F.

Single-cell Analysis of Human Primary Prostate Cancer Reveals the Heterogeneity of Tumor-associated Epithelial Cell States. ,
Nat Commun
13 , 141 , 2022

Cells

Prostate cells

Enzymes

Collagenase Type 4 : 1,000 u/ml

Medium

RPMI

Tumor

Human

Jeske, S. , Schuler, P. , Doescher, J. , Theodoraki, M. , Laban, S. , Brunner, C. , Hoffman, T. and Wigand, M.

Age-related changes in T lymphocytes of patients with head and neck squamous cell carcinoma ,
Immun Ageing
17 , 3 , 2020

Cells

Tumor infiltrating lympocytes

Enzymes

Collagenase Type 1 : 200 u/ml

Medium

RPMI

Tumor

Human

Ayuso, J. , Vitek, R. , Swick, A. , Skala, M. , Wisinski, K. , Kimple, R. , Lambert, P. and Beebe, D.

Effects of Culture Method on Response to EGFR Therapy in Head and Neck Squamous Cell Carcinoma Cells ,
Sci Rep
9 , 12480 , 2019

Cells

Fibroblasts

Enzymes

Collagenase Type 1 : 0.5%

Neutral Protease : 0.1%

Deoxyribonuclease I : 1,000 u/ml

Medium

FM Fibroblast media

Tumor

Human

Phan, N. , Hong, J. , Tofig, B. , Mapua, M. , Elashoff, D. , Moatamed, N. , Huang, J. , Memarzadeh, S. , Damoiseaux, R. and Soragni, A.

A Simple High-Throughput Approach Identifies Actionable Drug Sensitivities in Patient-Derived Tumor Organoids. ,
Commun Biol
2 , 78 , 2019

Cells

Tumor

Enzymes

Collagenase Type 4 : 200 u/ml

Medium

DMEM

Tumor

Human

Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al

Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
175 , 1972 , 2018

Cells

Organoids

Enzymes

Collagenase Type 4 : 200-300 u/ml

Medium

DMEM

Tumor

Human

Jiang, C. , Zhang, Q. , Shanti, R. , Shi, S. , Chang, T. , Carrasco, L. , Alawi, F. and Le, A.

Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells. ,
Stem Cells
35 , 2083-2094 , 2017

Cells

Ameloblastoma epithelial

Enzymes

Collagenase : 0.1%

Neutral Protease : 50 u/ml

Medium

MEM

Tumor

Human

Human, 25-81 yr

Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,

A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
17 , 30 , 2016

Cells

Brain tumor

Enzymes

Neutral Protease : 0.11 u/ml

Collagenase Type 4 : 0.05%

Hyaluronidase : 1,000 u/ml

Deoxyribonuclease I : 5 u/ml

Medium

HBSS

Tumor

Human

Pham, K. , Delitto, D. , Knowlton, A. , Hartlage, E. , Madhavan, R. , Gonzalo, D. , Thomas, R. , Behrns, K. , George, T. , Hughes, S. , Wallet, S. , Liu, C. and Trevino, J.

Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. ,
Am J Pathol
186 , 2016

Cells

Pancreatic cancer

Enzymes

Stemxyme 1 : 0.2%

Medium

HBSS

Tumor

Human

Baldan, V. , Griffiths, R. , Hawkins, R. and Gilham, D

Efficient and Reproducible Generation of Tumour-Infiltrating Lymphocytes for Renal Cell Carcinoma ,
Br J Cancer
112 , 1510 , 2015

Cells

Tumour infiltrating lymphocytes

Enzymes

Collagenase : 1%

Hyaluronidase : 1%

Deoxyribonuclease I : 3,000 u/ml

Medium

RPMI 1640

Tumor

Human

Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.

Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
, e52532 , 2015

Cells

Colon

Enzymes

Trypsin : 0.1%

Collagenase : 0.1%

Medium

HBSS

Tumor

Human

Liang, S. , Marti, T. , Dorn, P. , Froment, L. , Hall, S. , Berezowska, S. , Kocher, G. , Schmid, R. and Peng, R.

Blocking the Epithelial-to-Mesenchymal Transition Pathway Abrogates Resistance to Anti-Folate Chemotherapy in Lung Cancer. ,
Cell Death Dis
6 , e1824 , 2015

Cells

Non-small cell lung tumor

Enzymes

Collagenase Type 1 : 0.1%

Collagenase Type 2 : 0.25%

Medium

RPMI-1640

Tumor

Human

Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.

An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
97 , 201 , 2015

Cells

Lung tumor

Enzymes

Collagenase Type 1 : 45-60 u/ml

Collagenase Type 2 : 15-20 u/ml

Collagenase Type 4 : 45-60 u/ml

Elastase : 0.002%

Deoxyribonuclease I : 0.002%

Medium

DMEM/F12

Tumor

Human

Yan, K. , Wu, Q. , Yan, D. , Lee, C. , Rahim, N. , Tritschler, I. , DeVecchio, J. , Kalady, M. , Hjelmeland, A. and Rich, J.

Glioma Cancer Stem Cells Secrete Gremlin1 to Promote their Maintenance Within the Tumor Hierarchy. ,
Genes Dev
28 , 1085-100 , 2014

Cells

Glioma cancer stem

Enzymes

PDS kit : per instructions

Medium

Neurobasal

Tumor

Human

Hasselbach, L. , Irtenkauf, S. , Lemke, N. , Nelson, K. , Berezovsky, A. , Carlton, E. , Transou, A. , Mikkelsen, T. and deCarvalho, A.

Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. ,
J Vis Exp
, e51088 , 2014

Cells

Glioblastoma tumor

Enzymes

Trypsin : 0.025%

Collagenase Type 4 : 500 u/ml

Medium

HBSS

Tumor

Human

Dobbin, Z. and Landen, C.

Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
63 , Unit 14.28. , 2013

Cells

Cancer stem

Enzymes

Trypsin : 0.25%

Collagenase : 0.05%

Hyaluronidase : 0.005%

Medium

RPMI

Tumor

Human

Chou, J. , Fitzgibbon, M. , Mortales, C. , Towlerton, A. , Upton, M. , Yeung, R. , McIntosh, M. and Warren, E.

Phenotypic and Transcriptional Fidelity of Patient-Derived Colon Cancer Xenografts in Immune-Deficient Mice. ,
PLoS ONE
8 , e79874 , 2013

Cells

Tumor

Enzymes

Collagenase Type 1 : 0.1%

Hyaluronidase : 0.01%

Deoxyribonuclease I : 0.01%

Medium

DMEM/F12

Tumor

Human

Welte, Y. , Davies, C. , Schafer, R. and Regenbrecht, C.

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma. ,
J Vis Exp
73 , e50200 , 2013

Cells

Melanoma

Enzymes

Collagenase Type 4 : 0.1%

Deoxyribonuclease I : 0.01%

Medium

PBS

Tumor

Human

Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.

Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
13 , 70-83 , 2011

Cells

Brain tumor

Enzymes

Collagenase Type 1 : 0.04%

Hyaluronidase : 0.01%

Deoxyribonuclease I : 0.02%

Neutral Protease : 0.008%

Medium

DMEM/F12

Tumor

Human