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Tissue References Search Tool

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Neural
Avian
Quail and Chicken, embryonic
Rollo, B. , Zhang, D. , Simkin, J. , Menheniott, T. and Newgreen, D.
Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells. ,
F1000Res
 4 , 113 , 2015
Cells
Midgut DRG
Enzymes
Medium
Ham's F12
Neural
Avian
Chick, White Leghorn, embryos
Temburni, M. , Rosenberg, M. , Pathak, N. , McConnell, R. and Jacob, M.
Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein. ,
J Neurosci
 24 , 6776 , 2004
Cells
Cilary ganglion neurons
Enzymes
Medium
PBS
Neural
Bovine
Bovine
(also rat)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Neural
Bovine
Bovine
Bowman, P. , Betz, A. and Goldstein, G.
Primary Culture of Microvascular Endothelial Cells From Bovine Retina ,
In Vitro
 18 (7) , 626 , 1982
Cells
Microvascular endothelial
Enzymes
Medium
MEM
Neural
Bovine
Calf
(also lamb)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Neural
Canine
Canine
Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.
Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
 7 , 13915 , 2017
Cells
Satellite glial
Enzymes
Medium
HBSS
Neural
Chicken
Chick, White Leghorn, embryos
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Neural
Chicken
Chicken, White Leghorn, embryos, 17-21 day
Raman, I. and Trussell, L.
The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus ,
Neuron
 9 , 173 , 1992
Cells
Neurons
Enzymes
Papain : 40 u/ml
Medium
HEPES
Neural
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Neural
Chicken
Chick, White Leghorn, embryo
(also rat, SD, embryo)
Schnaar, R. and Schaffner, A.
Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions ,
J Neurosci
 1 , 204 , 1981
Cells
Spinal cord
Enzymes
Trypsin : 0.05%
Medium
Phosphate buffer
(see reference)
Neural
Chicken
Chick
Choi, D. and Fiscbach, G.
GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture ,
J Neurophysiol
 45 , 605 , 1981
Cells
Dorsal root ganglion neurons
Spinal cord
Enzymes
Trypsin : 0.1%
Medium
Puck's saline, CMF
Neural
Chicken
Chick, White Leghorn embryo, 8 day
Bottenstein, J. , Skaper, S. , Varon, S. and Sato, G.
Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium ,
Exp Cell Res
 125 , 183 , 1980
Cells
Neurons, ganglia
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Chicken
Chicken, White Leghorn, fertile eggs
Tuttle, J. , Suszkiw, J. and Ard, M.
Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture ,
Brain Res
 183 , 161 , 1980
Cells
Ciliary ganglion neurons
Enzymes
Trypsin : 0.25%
Medium
Eagle's MEM
Neural
Chicken
Chicken, embryos, 9-10 days old
Mudge, A. , Leeman, S. and Fischbach, G.
Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration ,
Proc Natl Acad Sci U S A
 76 (1) , 526 , 1979
Cells
Dorsal root ganglia neurons
Enzymes
Collagenase : 0.01%
Medium
Eagle's MEM
Neural
Chicken
Chick embryos
McCarthy, K. and Partlow, L.
Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates ,
Brain Res
 114 , 391 , 1976
Cells
Ganglion chains, sympathetic ganglia
Enzymes
Trypsin : 0.25%
Medium
Krebs Phosphosaline
Neural
Chicken
Chick, White Leghorn, embryos, 7 day
Wiseman, L. and Hammond, W.
The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
 197 , 429 , 1976
Cells
Neural retina
Enzymes
Collagenase : 0.25%
Elastase : 0.2%
Hyaluronidase : 1.0%
Papain : 1.0%
Protease : 0.1%
Trypsin : 0.05%
Medium
HBSS
Neural
Fish
Zebrafish, embryonic
Spiro, Z. , Koh, A. , Tay, S. , See, K. and Winkler, C.
Transcriptional Enhancement of Smn Levels in Motoneurons is Crucial for Proper Axon Morphology in Zebrafish. ,
Sci Rep
 6 , 27470 , 2016
Cells
Motorneurons
Enzymes
PDS kit : per instructions
Medium
L-15
Neural
Fish
Zebrafish
Sakowski, S. , Lunn, J. , Busta, A. , Palmer, M. , Dowling, J. and Feldman, E.
A Novel Approach to Study Motor Neurons from Zebrafish Embryos and Larvae in Culture. ,
J Neurosci Methods
 205 , 277-82 , 2012
Cells
Motor neurons
Enzymes
Medium
See reference
Neural
Fish
Zebrafish, Dania rerio, embryo
Won, Y. , Ono, F. and Ikeda, S.
Identification and Modulation of Voltage-Gated Ca2+ Currents in Zebrafish Rohon-Beard Neurons. ,
J Neurophysiol
 105 , 442-53 , 2011
Cells
Rohon-Beard neurons
Enzymes
Trypsin : 0.2%
Medium
L-15/Hepes
Neural
Fish
Zebrafish, Danio rerio, embryo
Cerda, G. , Hargrave, M. and Lewis, K.
RNA Profiling of FAC-sorted Neurons from the Developing Zebrafish Spinal Cord. ,
Dev Dyn
 238 , 150-61 , 2009
Cells
Neurons
Enzymes
PDS kit : with modifications
Medium
EBSS
Neural
Fish
Black ghose knige fish, adult (Apteronotus albifrons)
Anderson, M.J.
Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro ,
In Vitro Cell Dev Biol
 29A , 145 , 1993
Cells
Neurons, spinal cord
Enzymes
Trypsin : 0.4%
Medium
PBS, CMF
Neural
Frog
Xenopus, embryonic
Takahashi, T. , Nakajima, Y. , Hirosawa, K. , Nakajima, S. and Onodera, K.
Structure and Physiology of Developing Neuromuscular Synapses in Culture ,
J Neurosci
 7 , 473 , 1987
Cells
Neuron
Enzymes
Medium
Steinberg's solution
Neural
Guinea-Pig
Guinea-pig, newborn
Jessen, K, McConnell, J. , Purves, R. , Burnstock, G. and Chamley-Campbell, J.
Tissue Culture of Mammalian Enteric Neurons ,
Brain Res
 152 , 573 , 1978
Cells
Neuron, enteric
Enzymes
Trypsin : 0.125%
Medium
Medium 199
Neural
Hamster
Hamster, male
Liman ER.
Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons ,
J Physiol
 548 , 777 , 2003
Cells
Vomeronasal organ neurons
Enzymes
Trypsin : 0.02%
Medium
PBS
Neural
Human

Human

David, E. , Yousuf, M. , Mei, H. , Jain, A. , Krishnagiri, S. , Elahi, H. , Venkatesan, R. , Srikanth, K. , Dussor, G. , Dalva, M. and Price, T.
Ephrin-B2 promotes Nociceptive Plasticity and Hyperalgesic Priming Through EphB2-MNK-eIF4E Signaling in Both Mice and Humans. , Pharmacol Res Vol. 206 , 107284 , 2024
Cells
DRG neurons
Enzymes
Medium
HBSS
Neural
Human

Human

Park, S. , Eber, M. , Fonseca, M. , Patel, C. , Cunnane, K. , Ding, H. , Hsu, F. and Peters, C.
Usefulness of the Measurement of Neurite Outgrowth of Primary Sensory Neurons to Study Cancer-Related Painful Complications. ,
Biochem Pharmacol
 188 , 114520 , 2021
Cells
Dorsal root ganglia
Neural
Enzymes
Papain : 30 u/ml
Medium
HBSS
Neural
Human
Human
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Neural
Human
Human
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Human
Human, 9 month
Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.
EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy ,
Nat Med
 11 , 277 , 2005
Cells
Neurons
Enzymes
Papain : 0.07 u/ml
Neutral Protease : 1 mg/ml
Medium
DMEM/F12
Neural
Human
Human, 26 week
Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.
Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells ,
J Neurosci Res
 75 , 782 , 2004
Cells
Neural progenitor cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Neural
Human
Human, adult
Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA.
Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone ,
J Neurosci Res
 59 , 321 , 2000
Cells
Ventricular epithelial
Enzymes
Papain : 11.4 u/ml
Medium
DMEM/F12
Neural
Human
Human, 5-65 years
Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
 69 , 97 , 1999
Cells
Retinal pigment epithelial (RPE)
Enzymes
Trypsin : 0.25%
Medium
HBSS
Neural
Human
Human
Dib-Hajj SD, Tyrrell L, Cummins TR, Black JA, Wood PM, Waxman SG.
Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons ,
F.E.B.S. Lett.
 462 , 117 , 1999
Cells
Dorsal root ganglion neurons
Enzymes
Medium
DMEM/F-12
Neural
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Neural
Insect
Drosophila
Sanchez-Soriano, N. , Bottenberg, W. , Fiala, A. , Haessler, U. , Kerassoviti, A. , Knust, E. , Lohr, R. and Prokop, A.
Are Dendrites in Drosophila Homologous to Vertebrate Dendrites? ,
Dev Biol
 288 , 126 , 2005
Cells
Dendrites
Enzymes
Collagenase : 0.05%
Medium
HBSS
Neural
Insect
Gryllus Bimaculatus
Kloppenburg, P. and Horner, M.
Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus ,
J Exp Biol
 201 (Pt 17) , 2529 , 1998
Cells
Giant interneurons
Enzymes
Collagenase : 0.05%
Medium
Leibovitz's L15

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.