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Tissue References Search Tool

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Organoids
Human

Human

Gamero-Estevez, E. , Hensel, I. , Steinhauer, M. , Müllertz, O. , Savochkina, E. , Sektioglu, I. , Stoll, B. , Derakhshani, S. , Devriese, S. , Kim, K. and Resnik-Docampo, M.
Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine. ,
Bio Protoc
 15 , e5157 , 2025
Cells
Intestinal organoids
Enzymes
Medium
RPMI
Organoids
Human

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.
Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024
Cells
Cerebral organoids
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Organoids
Human

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.
Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
 15 , 7027 , 2024
Cells
Brain organoids
Enzymes
Papain : 20 u/ml
Medium
EBSS
Organoids
Human

Human

Tresenrider, A. , Sridhar, A. , Eldred, K. , Cuschieri, S. , Hoffer, D. , Trapnell, C. and Reh, T.
Single-cell Sequencing of Individual Retinal Organoids Reveals Determinants of Cell Fate Heterogeneity. , bioRxiv , 2023
Cells
Retinal Organoids
Enzymes
PDS kit : see reference
Medium
DMEM
Organoids
Human

Human

Chiu, M. , Li, C. , Yu, Y. , Liu, X. , Huang, J. , Wan, Z. , Yuen, K. and Zhou, J.
Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids. ,
Bio Protoc
 13 , e4657 , 2023
Cells
Lung Organoids
Enzymes
Medium
Basal medium
Organoids
Human

Human, adolescent

Hemeryck, L. , Hermans, F. , Chappell, J. , Kobayashi, H. , Lambrechts, D. , Lambrichts, I. , Bronckaers, A. and Vankelecom, H.
Organoids from Human Tooth Showing Epithelial Stemness Phenotype and Differentiation Potential. ,
Cell Mol Life Sci
 79 , 153 , 2022
Cells
Epithelial organoids
Enzymes
Medium
MEM
Organoids
Human

Human

Schröter, M. , Wang, C. , Terrigno, M. , Hornauer, P. , Huang, Z. , Jagasia, R. and Hierlemann, A.
Functional Imaging of Brain Organoids Using High-Density Microelectrode Arrays. , MRS Bull Vol. 47 , 530-544 , 2022
Cells
Cerebral organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Uzquiano, A. , Kedaigle, A. , Pigoni, M. , Paulsen, B. , Adiconis, X. , Kim, K. , Faits, T. , Nagaraja, S. , Antón-Bolaños, N. , Gerhardinger, C. , Tucewicz, A. , Murray, E. , Jin, X. , Buenrostro, J. , Chen, F. , Velasco, S. , Regev, A. , Levin, J. and Arlotta, P.
Proper Acquisition of Cell Class Identity in Organoids Allows Definition of Fate Specification Programs of the Human Cerebral Cortex ,
Cell
 185 , 3770-3788.e27 , 2022
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Vaart, J. , Böttinger, L. , Geurts, M. , Wetering, W. , Knoops, K. , Sachs, N. , Begthel, H. , Korving, J. , Lopez-Iglesias, C. , Peters, P. , Eitan, K. , Gileles-Hillel, A. and Clevers, H.
Modelling of Primary Ciliary Dyskinesia using Patient-derived Airway Organoids. , EMBO Rep Vol. 22 , e52058 , 2021
Cells
Airway organoids
Enzymes
Pronase : 0.15%
Collagenase : 0.05%
Medium
Airway medium
Organoids
Human

Human

Marinucci, M. , Ercan, C. , Taha-Mehlitz, S. , Fourie, L. , Panebianco, F. , Bianco, G. , Gallon, J. , Staubli, S. , Soysal, S. , Zettl, A. , Rauthe, S. , Vosbeck, J. , Droeser, R. , Bolli, M. , Peterli, R. , Flüe, M. , Ng, C. , Kollmar, O. , Coto-Llerena, M. and Piscuoglio, S.
Standardizing Patient-Derived Organoid Generation Workflow to Avoid Microbial Contamination From Colorectal Cancer Tissues. , Front Oncol Vol. 11 , 781833 , 2021
Cells
Colorectal cancer organoids
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Schreurs, R. , Baumdick, M. , Drewniak, A. and Bunders, M.
Co-culture of Human Intestinal Organoids and Lamina Propria-derived CD4 T Cells. , STAR Protoc Vol. 2 , 100519 , 2021
Cells
Lamina propria lymphocytes
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Cells
Astrocytes
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Mansour, A. , Gonçalves, J. , Bloyd, C. , Li, H. , Fernandes, S. , Quang, D. , Johnston, S. , Parylak, S. , Jin, X. and Gage, F.
An In Vivo Model of Functional and Vascularized Human Brain Organoids. ,
Nat Biotechnol
 36 , 432-441 , 2018
Cells
Cerebral organoids
Enzymes
Medium
DMEM/F12
Organoids
Human

Human, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
Organoids
Mouse

Mouse

Fujimura, T. , Enomoto, Y. , Katsura, H. , Ogawa, T. , Baba, S. , Ogata, A. , Yamaoka, A. , Shiroguchi, K. and Morimoto, M.
Identifying a Lung Stem Cell Subpopulation by Combining Single-Cell Morphometrics, Organoid Culture, and Transcriptomics. ,
Stem Cells
 41 , 809-820 , 2023
Cells
Lung
Enzymes
Collagenase Type 1 : 450 u/ml
Elastase : 1 u/ml
Neutral Protease : 5 u/ml
Medium
DMEM/F12
Organoids
Mouse

Mouse

Klemke, L. , Blume, J. , Oliveira, T. and Schulz-Heddergott, R.
Preparation and Cultivation of Colonic and Small Intestinal Murine Organoids Including Analysis of Gene Expression and Organoid Viability. ,
Bio Protoc
 12 , e4298 , 2022
Cells
Colonic tumor organoids
Enzymes
Medium
PBS
Organoids
Mouse

Mouse

Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Organoids
Mouse

Mouse

Charifou, E. , Sumbal, J. , Koledova, Z. , Li, H. and Chiche, A.
A Robust Mammary Organoid System to Model Lactation and Involution-like Processes. ,
Bio Protoc
 11 , e3996 , 2021
Cells
Mammary organoids
Enzymes
Trypsin : 0.2%
Medium
DMEM/F12
Organoids
Mouse

Mouse, adult

Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al
Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
 175 , 1972 , 2018
Cells
Organoids
Enzymes
Collagenase Type 4 : 200-300 u/ml
Medium
DMEM
Organoids
Mouse

Mouse, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
Organoids
Mouse

Mouse

O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
 6 , 2016
Cells
Intestinal organoids
Enzymes
Medium
Basal Medium
Organoids
Mouse

Mouse, 8-12 week

Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E.
Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. ,
EMBO J
 32 , 2708-21 , 2013
Cells
Pancreas organoid
Enzymes
Neutral Protease : 0.012%
Collagenase : 0.012%
Medium
DMEM
Organoids
Mouse

Mouse

Xue, X. and Shah, Y.
In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
 , e50210 , 2013
Cells
Colon tumor organoids
Enzymes
Medium
DMEM
Organoids
Mouse

Mouse

Barthel, E. , Speer, A. , Levin, D. , Sala, F. , Hou, X. , Torashima, Y. , Wigfall, C. and Grikscheit, T.
Tissue Engineering of the Intestine in a Murine Model. ,
J Vis Exp
 70 , e4279 , 2012
Cells
Intestinal organoids
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Pancreas
Bovine
Bovine
Cotton, C. and Al-Nakkash, L.
Isolation and Culture of Bovine Pancreatic Duct Epithelial cells ,
Am J Physiol
 272 , G1328 , 1997
Cells
Duct epithelial
Enzymes
Collagenase : 0.1%
Medium
HEPES
Pancreas
Bovine
Bovine (Bos taurus)
Sato, T. , Sato, M. , Hudson, E. and Jones, R.
Characterization of Bovine Pancreatic Ductal Cells Isolated by a Perfusion-Digestion Technique ,
In Vitro
 19 , 651 , 1983
Cells
Ductal
Enzymes
Medium
EBSS
Pancreas
Bovine
Bovine
Stiles, G. and Lefkowitz, R.
Hormone-Sensitive Adneylate Cyclase ,
J Biol Chem
 257 (11) , 6287 , 1982
Cells
Platelets
Medium
(see reference)
Pancreas
Canine
Canine
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Pancreas
Canine
Canine, adult mongrel, 15-25 kg
Noel, J. , Rabinovitch, A. , Olson, L. , Kyriakides, G. , Miller, J. and Mintz, D.
A Method for Large-Scale, High-Yield Isolation of Canine Pancreatic Islets of Langerhans ,
Metabolism
 31 (2) , 184 , 1982
Cells
Islets
Enzymes
Collagenase Type 4 : 600-1100 u/ml
Deoxyribonuclease I : 10 ug/ml
Medium
RPMI 1640
Pancreas
Fish
Fish, Osphronemus gourami, 3-month-old, 0.5 mg
Schrezenmeir, J. , Laue, C. , Sternheim,E. , Wolbert, K. , Darquy, S. , Chicheportiche, D. , Kirchgessner, J. and Reach, G.
Long-Term Function of Single-Cell Preparations of Piscine Principal Islets in Hollow Fibers ,
Transplant Proc
 24 (6) , 2941 , 1992
Cells
Islets
Enzymes
Collagenase : 0.12 - 0.46 u/ml
Medium
RPMI 1640
Pancreas
Guinea-Pig
Guinea-pig
Schultz, G. , Sarras, Jr, M. , Gunther, G. , Hull, B. , Alicea, H. , Gorelick, F. and Jamieson, J.
Guinea Pig Pancreatic Acini Prepared with Purified collagenase ,
Exp Cell Res
 130 , 49 , 1980
Cells
Acinar
Enzymes
Collagenase Type 3 : 60 u/ml
Medium
Kreb's Ringer
Pancreas
Guinea-Pig
Guinea-pig, Hartley, albino, male, 350-400 g
Gardner, J. , Conlon, T. , Klaeveman, H. , Adams, T. and Ondetti, M.
Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells ,
J Clin Invest
 56 , 366 , 1975
Cells
Acinar
Enzymes
Medium
Kreb's Ringer
Pancreas
Guinea-Pig
Guinea-pig
Amsterdam, J. and Jamieson, J.
Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells ,
Proc Natl Acad Sci U S A
 69 (10) , 3028 , 1972
Cells
Exocrine
Enzymes
Hyaluronidase : 0.15% - 0.2%
Medium
Kreb's Ringer
Pancreas
Hamster
Hamster
Feldman, J. and Chapman, B.
Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique ,
Acta Diabetol
 12 , 208 , 1975
Cells
Islets
Enzymes
Collagenase Type 4 : 1.3% - 2.0%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.