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Tissue References Search Tool

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Mammary
Mouse
Mouse (BALB/cCrgl), mature
Bandyopadhyay, G. , Imagawa, W. , Wallace, D. and Nandi, S.
Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture ,
J Biol Chem
 263 , 7567 , 1988
Cells
Epithelial
Enzymes
Pronase : 0.01%
Medium
Hepes buffered medium 199
Mammary
Mouse
Mouse, BALB/c, female, 6-8 week
Ehmann, U. , Peterson, W. and Misfeldt, D.
To Grow Mouse Mammary Epithelial Cells in Culture ,
J Cell Biol
 98 , 1026 , 1984
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Mouse
Mouse (C3H/HeN), female
Taketani, Y. and Oka, T.
EGF Stimulates Cell Proliferation and Inhibits Functional Differentiation of Mouse Mammalian Dipithelial Cells in Culture ,
Endocrinology
 113 , 871 , 1983
Cells
Epithelial
Enzymes
Medium
Medium 199
Mammary
Mouse
Mouse, (BALB/c/Crgl Me), female, pregnant
Riser, M. , Huff, B. and Medina, D.
Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
 19 , 730 , 1983
Cells
Epithelial
Enzymes
Pepsin : 0.1% and 0.05%
Pepsin : 0.1%
Medium
HBSS with 0.2% EDTA, CMF
Mammary
Mouse
Mouse, female
Jones, W. and Hallowes, R.
Isolation of the Epithelial Subcomponents of the Mouse Mammary Gland for Tissue-Level Culture Studies ,
J Tiss Cul Meth
 8 (1) , 17 , 1983
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Mouse
Mouse (NMuMG), female, 2 months
Ehmann, U. and Misfeldt, D.
Mouse Mammary Cells in D-Valine Medium ,
In Vitro
 18 , 407 , 1982
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Mouse
Mouse, BALB/cCr1, female
Asch, B. , Burstein, N. , Vidrich, A. and Sun, T.
Identification of Mouse Mammary Epithelial Cells by Immunofluorescence With Rabbit and Guinea-Pig Antikeratin Antisera ,
Proc Natl Acad Sci U S A
 78 , 5643 , 1981
Cells
Epithelial
Enzymes
Medium
HBSS/DMEM
Mammary
Mouse
Mouse (BALB/cfC3H)
Yang, J. , Guzman, R. , Richards, J. and Nandi, S.
Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels ,
In Vitro
 16 , 502 , 1980
Cells
Mammary tumors
Epithelial
Enzymes
Collagenase : 1.0%
Medium
HBSS
Mammary
Mouse
Mouse, pregnant 8-12 day
Yang J, Richards J, Guzman R, Imagawa W. and Nandi S.
Sustained growth in primary culture of normal mammary epithelial cells embedded in collagen gels ,
Proc Natl Acad Sci U S A
 77 , 2088 , 1980
Cells
Mammary
Medium
HBSS
Mammary
Mouse
Mouse (BALB/c), pregnant, 60-80 days
Ceriani, R. , Peterson, J. and Abraham, S.
Removal of Cell Surface Material by Enzymes Used to Dissociate Mammary Gland Cells ,
In Vitro
 14 , 887 , 1978
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
CMF medium
Mammary
Mouse
Mouse, BALB/cfC3H/Crgl, virgin, female
DeOme, K. , Miyamoto, M. , Osborn, R. , Guzman, R. and Lum, K.
Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice ,
Cancer Res
 38 , 2103 , 1978
Cells
Nodule-transformed
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Mouse
Mouse (BALB/cCrgl), virgin, female, 4-8 month
White, M. , Hu, A. , Hamamoto, S. and Nandi
In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: fibroblast-free growth and serial passage ,
In Vitro
 14 , 271 , 1978
Cells
Epithelial
Mammary gland
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, BALB/cCrgl, female
(also guinea-pig)
Asch, B. and Medina, D.
Concanavalin A-Induced Agglutinability of Normal, Preneoplastic, and Neoplastic Mouse Mammary Cells ,
J Natl Cancer Inst
 61 (6) , 1423 , 1978
Cells
Swiss 3T3
Enzymes
Medium
PBS CMF
Mammary
Mouse
Mouse, (C3H/Crgl or BALB/cCrgl), 8-10 day pregnant
Emerman, J. , Enami, J. , Pitelka, D. and Nandi, S.
Hormonal Effects on Intracellular and Secreted Casein in Cultures of Mouse Mammary Epithelial Cells on Floating Collagen Membranes ,
Proc Natl Acad Sci U S A
 74 (10) , 4466 , 1977
Cells
Epithelial
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, lactating
Kerkof, P. and Abraham, S.
Preparation of Adipose Cell-Free Suspensions of Mammary Gland Parenchymal Cells from Lactating Mice ,
Methods Enzymol
 69 , 693 , 1976
Cells
Parenchymal
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Mammary
Mouse
Mouse, female, early pregnancy <10 days
Moore, D. and Lasfargues, E.
Method for the Continuous Cultivation of Mammary Epithelium ,
In Vitro
 7 , 21 , 1971
Cells
Epithelial
Enzymes
Collagenase : 0.1%
Medium
Eagle's MEM
Mammary
Mouse
Mouse, CBA, virgin, 9/10 wk
Prop, F. and Wiepjes, G.
Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse ,
Exp Cell Res
 61 , 451 , 1970
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
BSS, CMF
Mammary
Mouse
Mouse, lactating, 14-18 day
Pitelka, D. , Kerkof, P. , Gagne, H. , Smith, S. and Abraham, S.
Characteristics of Cells Dissociated from Mouse Mammary Glands. I. Method of Separation and Morphology of Parenchymal Cells from Lactating Glands ,
Exp Cell Res
 57 , 43 , 1969
Cells
Parenchymal
Enzymes
Collagenase : 0.33%
Medium
Kreb's buffer
Mammary
Mouse
Mouse, lactating, 14 day
Kopelovich, L. , Abraham, S. , McGrath, H. , DeOme, K. and Chaikoff, I.
Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland ,
Cancer Res
 26 , 1534 , 1966
Cells
Mammary
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, pregnant, 14-17 day
Daniel, C. and DeOme, K.
Growth of Mouse Mammary Glands In Vivo After Monolayer Culture ,
Science
 149 , 634 , 1965
Cells
Mammary
Enzymes
Collagenase : 0.05% - 0.1%
Medium
HBSS
Mammary
Mouse
Mouse, adult, 1-10 days pregnant
Lasfargues, E.
Cultivation and Behavior In Vitro of the Normal Mammary Epithelium of the Adult Mouse. II. Observations on the Secretory Activity ,
Exp Cell Res
 13 , 553 , 1957
Cells
Epithelial
Mammary
Enzymes
Collagenase : 0.02%
Medium
Simm's
Miscellaneous
Goat
Goat
Arora, A. , Sriram, M. , Kothari, A. and Katti, D.
Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering. ,
Cytotherapy
 19 , 881-894 , 2017
Cells
Infrapatellar fat pad mesenchymal stromal
Enzymes
Collagenase : 0.15%
Medium
DMEM
Miscellaneous
Mouse

Mouse

Hameed, M. , Rai, P. , Makris, M. and Weger-Lucarelli, J.
Optimized Protocol for Mouse Footpad Immune Cell Isolation for Single-cell RNA Sequencing and Flow Cytometry. ,
Star Protocols
 4 , 102409 , 2023
Cells
Leucocytes
Enzymes
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse
Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Miscellaneous
Mouse
Mouse, embryonic
He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.
Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
 9 , 53085 , 2020
Cells
Trachea
Enzymes
Elastase : 0.1%
Trypsin : 0.125%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse
Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C.
Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. ,
Nat Commun
 11 , 4061 , 2020
Cells
Adhesion fibroblasts
Enzymes
Medium
Medium 199
Miscellaneous
Mouse
Mouse, 8-12 week
Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
 , 2020
Cells
Urinary bladder
Enzymes
Collagenase Type 1 : 50 u/ml
Collagenase Type 3 : 40 u/ml
Papain : 45 u/ml
Collagenase Type 6 : 20 u/ml
Medium
Tyrode solution
Miscellaneous
Mouse
Mouse
Ishida, T. , Suzuki, S. , Lai, C. , Yamazaki, S. , Kakuta, S. , Iwakura, Y. , Nojima, M. , Takeuchi, Y. , Higashihara, M. , Nakauchi, H. and Otsu, M.
Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells. ,
Stem Cells
 35 , 989-1002 , 2017
Cells
Bone marrow stromal
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse
Huang, C. , Chen, C. and Shyr, C.
The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer. ,
Cytotherapy
 19 , 1233-1245 , 2017
Cells
Urothelial
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse
Kim, Y. , Wang, S. , Tymanskyj, S. , Ma, L. , Tao, H. and Zhang, L.
Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. ,
Sci Rep
 6 , 23799 , 2016
Cells
Cochlear
Enzymes
Papain : 20 u/ml
Trypsin : 0.05%
Medium
DMEM
Miscellaneous
Mouse
Mouse
Chavez, M. , Hu, J. , Seidel, K. , Li, C. , Jheon, A. , Naveau, A. , Horst, O. and Klein, O.
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor. ,
J Vis Exp
 , 2014
Cells
Epithelial stem
Enzymes
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse, 10 week
Futami, I. , Ishijima, M. , Kaneko, H. , Tsuji, K. , Ichikawa-Tomikawa, N. , Sadatsuki, R. , Muneta, T. , Arikawa-Hirasawa, E. , Sekiya, I. and Kaneko, K.
Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium. ,
PLoS ONE
 7 , e45517 , 2012
Cells
Synovial mesenchymal
Enzymes
Medium
DMEM
Miscellaneous
Mouse
Mouse, 26-30 g
Smith, T. , Grider, J. , Dewey, W. and Akbarali, H.
Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels. ,
PLoS ONE
 7 , e45251 , 2012
Cells
Myenteric plexus
Enzymes
Medium
Neurobasal A
Miscellaneous
Mouse
Mouse
Jan, T. , Chai, R. , Sayyid, Z. and Cheng, A.
Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry. ,
J Vis Exp
 58 , e3432 , 2011
Cells
Cochleaer
Enzymes
Trypsin : 0.125%
Medium
DMEM/F-12
Miscellaneous
Mouse
Mouse
Stock, A. , Booth, S. and Cerundolo, V.
Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans. ,
J Exp Med
 208 , 761 , 2011
Cells
Dentritic
Enzymes
Medium
DME
Miscellaneous
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.