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Adipose/Fat
Mouse

Mouse, Adult

Hay, A. , Murphy, T. , Popichak, K. , Zabel, M. and Moreno, J.
Adipose-derived mesenchymal stromal cells decrease prion-induced glial inflammation in vitro. ,
Sci Rep
 12 , 22567 , 2022
Cells
Mesenchymal stromal
Enzymes
Stemxyme 1 : 400u/ml
Medium
DMEM/F12
Adipose/Fat
Mouse
Mouse
Jones, J. , Rabhi, N. , Orofino, J. , Gamini, R. , Perissi, V. , Vernochet, C. and Farmer, S.
The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. ,
Sci Rep
 10 , 2380 , 2020
Cells
Adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Bowles, A. , Strong, A. , Wise, R. , Thomas, R. , Gerstein, B. , Dutreil, M. , Hunter, R. , Gimble, J. and Bunnell, B.
Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis. ,
Stem Cells
 35 , 532-544 , 2017
Cells
Adipose stromal
Enzymes
Medium
HBSS
Adipose/Fat
Mouse
Mouse
Yao, W. , Lay, Y. , Kot, A. , Liu, R. , Zhang, H. , Chen, H. , Lam, K. and Lane, N.
Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference. ,
Stem Cells
 34 , 2587-2600 , 2016
Cells
Adipose stromal
Enzymes
Medium
PBS
Adipose/Fat
Mouse
Mouse
Maria, O. , Shalaby, M. , Syme, A. , Eliopoulos, N. and Muanza, T.
Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis. ,
Cytotherapy
 18 , 1129-45 , 2016
Cells
Adipose mesenchyal stromal
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Jang, H. , Bhasin, S. , Guarneri, T. , Serra, C. , Schneider, M. , Lee, M. , Guo, W. , Fried, S. , Pencina, K. and Jasuja, R.
The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life. ,
Endocrinology
 156 , 3737 , 2015
Cells
Adipocytes
Enzymes
Collagenase : 0.1%
Medium
Krebs
Adipose/Fat
Mouse
Mouse
Seaman, S. , Tannan, S. , Cao, Y. , Peirce, S. and Lin, K.
Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts. ,
Plast Reconstr Surg
 136 , 189e-199e , 2015
Cells
Adipocytes
Enzymes
Medium
PBS
Adipose/Fat
Mouse
Mouse, 20 week
Kondo, T, Toyoshima, Y. , Ishii, Y. and Kyuwa, S.
Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice. ,
Exp Anim
 62 , 319 , 2013
Cells
Stromal vascular
Enzymes
Medium
RPMI-1640
Adipose/Fat
Mouse
Mouse
Takahashi, H. , Haraguchi, N. , Nishikawa, S. , Miyazaki, S. , Suzuki, Y. , Mizushima, T. , Nishimura, J. , Takemasa, I. , Yamamoto, H. , Mimori, K. , Ishii, H. , Doki, Y. and Mori, M.
Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair. ,
Stem Cells Transl Med
 1 , 803 , 2012
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Han, J. , Koh, Y. , Moon, H. , Ryoo, H. , Cho, C. , Kim, I. and Koh, G.
Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells. ,
Blood
 115 , 957 , 2010
Cells
Stem and progenitor
Enzymes
Medium
HBSS
Adipose/Fat
Mouse
Mouse
Wong, K. , Szeto, F, Zhang, W. , Ye, H. , Kong, J. , Zhang, Z. , Sun, X. and Li, Y
Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins. ,
Am J Physiol/Endo
 296 , 820 , 2009
Cells
White adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Thangarajah, H. , Vial, I. , Chang, E. , El-Ftesi, S. , Januszyk, M. , Chang, E. , Paterno, J. , Neofytou, E. , Longaker, M. and Gurtner, G.
IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia. ,
Stem Cells
 27 , 266 , 2009
Cells
Adipose stromal
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse, 3 week
De Matteis, R. , Zingaretti, M. , Murano, I. , Vitali, A. , Frontini, A. , Giannulis, I. , Barbatelli, G. , Marcucci, F. , Bordicchia, M. , Sarzani, R. , Raviola, E. and Cinti, S.
In Vivo Physiological Transdifferentiation of Adult Adipose Cells. ,
Stem Cells
 27 , 2761 , 2009
Cells
Adipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Mouse
Mouse
Cho, K. , Park, H. , Park, H. , Jung, J. , Jeon, S. , Kim, Y. and Roh, H.
IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model. ,
Stem Cells
 27 , 259-65 , 2009
Cells
Adipose tissue-derived stem
Enzymes
Medium
Modified Eagles
Adipose/Fat
Mouse
Mouse, C57Bl/6J
Aoyagi T, Shimba S, Tezuka M.
Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes ,
J Health Sci
 51 , 21 , 2005
Cells
Adipocytes
Enzymes
Medium
DMEM/F12
Adipose/Fat
Mouse
Mouse
Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis.
Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives ,
Circulation
 109 , 656-63 , 2004
Cells
Stromal vascular, adipocytes
Enzymes
Collagenase : 0.2%
Medium
DMEM/F12
Adipose/Fat
Mouse
Mouse, 3-6 day and 2-3 month
Cowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T.
Adipose-derived adult stromal cells heal critical-size mouse calvarial defects ,
Nat Biotechnol
 22 , 560-7 , 2004
Cells
Adipose-derived stromal cells
Enzymes
Medium
PBS
Adipose/Fat
Mouse
Mouse, C57BL/6J and FVB, male, 8-9 week
Ruan, H. , Zarnowski, MJ. , Cushman, S. and Lodish, H.
Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes ,
J Biol Chem
 278 , 47585 , 2003
Cells
Adipocytes
Enzymes
Collagenase : see reference
Medium
DMEM
Adipose/Fat
Mouse
Mouse, both sexes
Nadler, S. , Stoehr, J. , Rabaglia, M. , Schueler, K. , Birnbaum, M. and Attie, A.
Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice ,
Am J Physiol/Endo
 281 , E1249 , 2001
Cells
Adipocytes
Enzymes
Collagenase : 0.05%
Medium
Krebs-Ringer Phosphate HEPES (KRPH)
Adipose/Fat
Mouse
Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male
Launder, T. , Gegen, N. , Knedler, A. and Harbeck, R.
The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue ,
J Immunol Methods
 102 , 45 , 1987
Cells
Vascular endothelial
Enzymes
Collagenase : 0.2%
Medium
PBS
Epithelial
Mouse
Mouse, 8-12 week
Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.
Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
 6 , 2020
Cells
Nasal epithelium
Enzymes
Papain : 20 u/ml
Medium
EBSS
Epithelial
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J.
Parent Stem Cells Can Serve as Niches for Their Daughter Cells. ,
Nature
 523 , 597-601 , 2015
Cells
Tracheal epithelial
Enzymes
Papain : 50 u/ml
Medium
See reference
Epithelial
Mouse
Mouse, adult
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1H.4 , 2011
Cells
Cilary epithelial
Enzymes
PDS kit : per instructions
Medium
EBSS
Epithelial
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Epithelial
Mouse
Mouse
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
 446 , 77-106 , 2008
Cells
Epithelial
Enzymes
Collagenase : 0.025%
Medium
DMEM
Epithelial
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Epithelial
Mouse
Mouse, female
Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y.
Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis ,
Am J Pathol
 155 , 173-81 , 1999
Cells
Salivary gland epithelial
Enzymes
Collagenase Type 1 : 750 u/ml
Hyaluronidase : 500 u/ml
Medium
DMEM/F12
Epithelial
Mouse
Mouse, fetal 12-13 day
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Mouse
Mouse
Durban, E.
Submandibular Salivary Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:2.1 , 1995
Cells
Submandibular salivary
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Katayama, M. and Kan, M.
Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation ,
In Vitro Cell Dev Biol
 27 , 533 , 1991
Cells
Esophageal
Enzymes
Trypsin : 0.25%
Medium
PBS, CMF
Epithelial
Mouse
Mouse, mature, female, 6-8-wk-old
Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.
Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes ,
In Vitro Cell Dev Biol
 27A , 713 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
HBSS
Epithelial
Mouse
Mouse, female, 18 - 20 days
(also 20 - 22 days)
Fukamachi, H. and McLachlan, J.
Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum ,
In Vitro Cell Dev Biol
 27A , 907 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.25%
Medium
HBSS
Epithelial
Mouse
Mouse, (BALB/c), male, 3-5 months
Durban, E.M.
Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix ,
In Vitro Cell Dev Biol
 26 , 33 , 1990
Cells
Epithelial
Submandibular salivary gland
Enzymes
Collagenase Type 3 : 0.16% , 1:1 v/v
Medium
DMEM with 15 mM HEPES
Epithelial
Mouse
Mouse, female
Riser, M. , Huff, B. and Medina, D.
Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
 19 , 730 , 1983
Cells
Epithelial
Enzymes
Pepsin : 0.1% and 0.05%
Pepsin : 0.1%
Medium
HBSS with 0.2% EDTA, CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.