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Tissue References Search Tool

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Epithelial
Mouse
Mouse, 8-12 week
Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.
Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
 6 , 2020
Cells
Nasal epithelium
Enzymes
Papain : 20 u/ml
Medium
EBSS
Epithelial
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J.
Parent Stem Cells Can Serve as Niches for Their Daughter Cells. ,
Nature
 523 , 597-601 , 2015
Cells
Tracheal epithelial
Enzymes
Papain : 50 u/ml
Medium
See reference
Epithelial
Mouse
Mouse, adult
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1H.4 , 2011
Cells
Cilary epithelial
Enzymes
PDS kit : per instructions
Medium
EBSS
Epithelial
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Epithelial
Mouse
Mouse
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
 446 , 77-106 , 2008
Cells
Epithelial
Enzymes
Collagenase : 0.025%
Medium
DMEM
Epithelial
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Epithelial
Mouse
Mouse, female
Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y.
Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis ,
Am J Pathol
 155 , 173-81 , 1999
Cells
Salivary gland epithelial
Enzymes
Collagenase Type 1 : 750 u/ml
Hyaluronidase : 500 u/ml
Medium
DMEM/F12
Epithelial
Mouse
Mouse, fetal 12-13 day
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Mouse
Mouse
Durban, E.
Submandibular Salivary Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:2.1 , 1995
Cells
Submandibular salivary
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Katayama, M. and Kan, M.
Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation ,
In Vitro Cell Dev Biol
 27 , 533 , 1991
Cells
Esophageal
Enzymes
Trypsin : 0.25%
Medium
PBS, CMF
Epithelial
Mouse
Mouse, mature, female, 6-8-wk-old
Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.
Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes ,
In Vitro Cell Dev Biol
 27A , 713 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
HBSS
Epithelial
Mouse
Mouse, female, 18 - 20 days
(also 20 - 22 days)
Fukamachi, H. and McLachlan, J.
Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum ,
In Vitro Cell Dev Biol
 27A , 907 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.25%
Medium
HBSS
Epithelial
Mouse
Mouse, (BALB/c), male, 3-5 months
Durban, E.M.
Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix ,
In Vitro Cell Dev Biol
 26 , 33 , 1990
Cells
Epithelial
Submandibular salivary gland
Enzymes
Collagenase Type 3 : 0.16% , 1:1 v/v
Medium
DMEM with 15 mM HEPES
Epithelial
Mouse
Mouse, female
Riser, M. , Huff, B. and Medina, D.
Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
 19 , 730 , 1983
Cells
Epithelial
Enzymes
Pepsin : 0.1% and 0.05%
Pepsin : 0.1%
Medium
HBSS with 0.2% EDTA, CMF
Epithelial
Mouse
Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant
Yang, J. , Flynn, D. , Larson, L. and Hamamoto, S.
Growth in Primary Culture of Mouse Submandibular Epithelial Cells ,
In Vitro
 18 , 435 , 1982
Cells
Epithelial
Submandibular gland
Enzymes
Medium
HBSS
Epithelial
Mouse
Mouse (BALB/cfC3H)
Yang, J. , Guzman, R. , Richards, J. and Nandi, S.
Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels ,
In Vitro
 16 , 502 , 1980
Cells
Mammary tumors
Epithelial
Enzymes
Collagenase : 1.0%
Medium
HBSS
Epithelial
Mouse
Mouse, C3H, 6-8 weeks
Lillehaug, J. , Mondal, S. and Heidelberger, C.
Establishment of Epithelial Cell Lines from Mouse Regenerating Liver ,
In Vitro
 15 , 910 , 1979
Cells
Epithelial
Enzymes
Collagenase : 0.10%
Medium
DMEM
Intestine
Mouse

Mouse

Kumar, S. , Suman, S. , Li, H. , Angdisen, J. , Datta, K. and Fornace, A.
High-linear Energy Transfer Radiation Disrupts Natural Killer Cell Surveillance of Senescent Intestinal Cells in the Mouse Intestine. , Mol Biomed Vol. 7 , 2026
Cells
Organoids
Enzymes
Medium
HBSS
Intestine
Mouse

Mouse, adult

Kumar, S. , Suman, S. , Angdisen, J. , Moon, B. , Kallakury, B. , Datta, K. and Fornace, A.
Effects of High-Linear-Energy-Transfer Heavy Ion Radiation on Intestinal Stem Cells: Implications for Gut Health and Tumorigenesis. ,
Cancers
 16 , 2024
Cells
Intestinal stem
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse
Emgard, J. , Kammoun, H. , Garcia-Cassani, B. , Chesne, J. , Parigi, S. , Jacob, J. , Cheng, H, Evren, E. , Das, S. , Czarnewski, P. , Sleiers, N. Melo-Gonzalez, F. , Kvedaraite, E. Svensson, M. and Scandella, E.
Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. ,
Immunity
 48 , 120-132.e8 , 2018
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse, embryonic
Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J. and Ming L
Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase. ,
Braz J Med Biol Res
 50 , e5831 , 2017
Cells
Crypt
Enzymes
Collagenase Type 1 : 200 u/ml
Hyaluronidase : 100u/ml
Medium
DMEM
Intestine
Mouse
Mouse
O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
 6 , 2016
Cells
Intestinal organoids
Enzymes
Medium
Basal Medium
Intestine
Mouse
Mouse
Seo, S. , Kuffa, P. , Kitamoto, S. , Nagao-Kitamoto, H. , Rousseau, J. , Kim, Y. , Nunez, G. and Kamada, N.
Intestinal Macrophages Arising from CCR2(+) Monocytes Control Pathogen Infection by Activating Innate Lymphoid Cells. ,
Nat Commun
 6 , 8010 , 2015
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Intestine
Mouse
Mouse
Beura, L. , Anderson, K. , Schenkel, J. , Locquiao, J. , Fraser, K. , Vezys, V. , Pepper, M. and Masopust, D.
Lymphocytic Choriomeningitis Virus Persistence Promotes Effector-Like Memory Differentiation and Enhances Mucosal T cell Distribution. ,
J Leukoc Biol
 97 , 217 , 2015
Cells
Lymphocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
RPMI-1640
Intestine
Mouse
Zhang, Y. and Hu, W.
Mouse Enteric Neuronal Cell Culture. ,
Methods Mol Biol
 1078 , 55-63 , 2013
Cells
Enteric neurons
Enzymes
Trypsin : 0.05%
Deoxyribonuclease I : 400 u/ml
Medium
DMEM/F12
Intestine
Mouse
Mouse, P4
Hotta, R. , Stamp, L. , Foong, J. , McConnell, S. , Bergner, A. , Anderson, R. , Enomoto, H. , Newgreen, D. , Obermayr, F. , Furness, J. and Young, H.
Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon. ,
J Clin Invest
 123 , 1182 , 2013
Cells
Enteric neural crest progenitors
Enzymes
Medium
DMEM/F12
Intestine
Mouse
Mouse
Xue, X. and Shah, Y.
In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
 , e50210 , 2013
Cells
Colon tumor organoids
Enzymes
Medium
DMEM
Intestine
Mouse
Mouse
Geem, D. , Medina-Contreras, O. , Kim, W. , Huang, C. and Denning T.
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine. ,
J Vis Exp
 63 , e4040 , 2012
Cells
Dendritic, macrophages
Enzymes
Collagenase : 0.15%
Medium
HBSS
Intestine
Mouse
Mouse, 2-3 month
Becker, L. , Kulkarni, S. , Tiwari, G. , Micci, M. and Pasricha, P.
Divergent Fate and Origin of Neurosphere-Like Bodies from Different Layers of the Gut. ,
Am J Physiol Gastrointest Liver Physiol
 302 , G958-65 , 2012
Cells
Enteric neural stem cells
Enzymes
Medium
M199
Intestine
Mouse
Mouse
Barthel, E. , Speer, A. , Levin, D. , Sala, F. , Hou, X. , Torashima, Y. , Wigfall, C. and Grikscheit, T.
Tissue Engineering of the Intestine in a Murine Model. ,
J Vis Exp
 70 , e4279 , 2012
Cells
Intestinal organoids
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Intestine
Mouse
Mouse
Ruiz, V. , Sachdev, M. , Zhang, S. , Wen, S. and Moss, S.
Isolating, Immunophenotyping and Ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes During Murine Helicobacter Pylori Infection. ,
J Immunol Methods
 384 , 157-63 , 2012
Cells
Lyphocytes
Enzymes
Medium
RPMI 1640
Intestine
Mouse
Mouse, neonatal and adult
Joseph, N. , He, S. , Quintana, E. , Kim, Y. , Nunez, G. and Morrison, S.
Enteric Glia are Multipotent in Culture but Primarily Form Glia in the Adult Rodent Gut. ,
J Clin Invest
 121 , 3398 , 2011
Cells
Myenteric plexus
Enzymes
Collagenase Type 4 : 0.025-0.1%
Papain : 10 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
HBSS
Intestine
Mouse
Mouse
Ito, Y. , Kanai, T. , Totsuka, T. , Okamoto, R. , Tsuchiya, K. , Nemoto, Y. , Yoshioka, A. , Tomita, T. , Nagaishi, T. , Sakamoto, N. , Sakanishi, T. , Okumura, K. , Yagita, H. and Watanabe, M.
Blockade of NKG2D Signaling Prevents the Development of Murine CD4+ T Cell-Mediated Colitis. ,
Am J Physiol Gastrointest Liver Physiol
 294 , G199 , 2008
Cells
Lamina propria lymphocytes
Enzymes
Medium
RPMI 1640
Intestine
Mouse
Mouse
Forbes, E. , Groschwitz, K. , Abonia, J.P. , Brandt, E. , Cohen, E. , Blanchard, C. , Ahrens, R. , Seidu, L. , McKenzie, A. , Strait, R. , Finkelman, F. , Foster, P. , Matthaei, K. , Rothenberg, M. and Hogan, S.
IL-9- and Mast Cell-Mediated Intestinal Permeability Predisposes to Oral Antigen Hypersensitivity. ,
J Exp Med
 205 , 897 , 2008
Cells
Intestinal mononuclear
Enzymes
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.