Tissue References Search Tool

Mouse

Mouse, 8-12 week

Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.

Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
6 , 2020

Cells

Nasal epithelium

Enzymes

Papain : 20 u/ml

Deoxyribonuclease I : 0.005%

Medium

EBSS

Epithelial

Mouse

Sun, F. , Xiao, G. and Qu, Z.

Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
7 , 2017

Cells

Alveolar epithelial

Enzymes

Neutral Protease : 0.1%

Deoxyribonuclease I : 0.01%

Medium

DMEM

Epithelial

Mouse

Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J.

Parent Stem Cells Can Serve as Niches for Their Daughter Cells. ,
Nature
523 , 597-601 , 2015

Cells

Tracheal epithelial

Enzymes

Papain : 50 u/ml

Medium

See reference

Epithelial

Mouse

Mouse, adult

Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.

Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
Chapter 1 , Unit 1H.4 , 2011

Cells

Cilary epithelial

Enzymes

PDS kit : per instructions

Medium

EBSS

Epithelial

Mouse

Mouse, male, 8-16 week

Breggia, A. and Himmelfarb, J.

Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
1 , 33 , 2008

Cells

Renal tubular epithelial

Enzymes

Collagenase : 200 u/ml

Soybean Trypsin Inhibitor : see reference

Medium

HBSS

Epithelial

Mouse

Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.

Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
446 , 77-106 , 2008

Cells

Epithelial

Enzymes

Collagenase : 0.025%

Neutral Protease : 0.25%

Medium

DMEM

Epithelial

Mouse

Mouse, 6-8 week

Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.

Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
168(1) , 411 , 2002

Cells

Lamina propria mononuclear cells

Enzymes

Collagenase Type 2 : 0.015%

Deoxyribonuclease I : 0.01%

Medium

RPMI

Epithelial

Mouse

Mouse, 11 week

Mueller, S. , Clark, J. , Myers, P. and Korach, K.

Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
143 , 2357 , 2002

Cells

Epithelial

Enzymes

Collagenase Type 3 : 25 u/ml

Hyaluronidase : 0.1%

Protease XIV : 0.05%

Deoxyribonuclease I : 0.04%

Medium

DMEM/F12

Epithelial

Mouse

Mouse, female

Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y.

Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis ,
Am J Pathol
155 , 173-81 , 1999

Cells

Salivary gland epithelial

Enzymes

Collagenase Type 1 : 750 u/ml

Hyaluronidase : 500 u/ml

Medium

DMEM/F12

Epithelial

Mouse

Mouse, fetal 12-13 day

Simon-Assmann, P. and Kedinger, M

Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995

Cells

Intestinal mesenchymal and epithelial

Enzymes

Collagenase : 0.03%

Medium

DMEM

Epithelial

Mouse

Durban, E.

Submandibular Salivary Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:2.1 , 1995

Cells

Submandibular salivary

Enzymes

Collagenase Type 2 or 3 : 0.16%

Medium

DMEM

Epithelial

Mouse

Katayama, M. and Kan, M.

Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation ,
In Vitro Cell Dev Biol
27 , 533 , 1991

Cells

Esophageal

Enzymes

Trypsin : 0.25%

Medium

PBS, CMF

Epithelial

Mouse

Mouse, mature, female, 6-8-wk-old

Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.

Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes ,
In Vitro Cell Dev Biol
27A , 713 , 1991

Cells

Uterine

Enzymes

Trypsin : 0.2%

Medium

HBSS

Epithelial

Mouse

Mouse, female, 18 - 20 days
(also 20 - 22 days)

Fukamachi, H. and McLachlan, J.

Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum ,
In Vitro Cell Dev Biol
27A , 907 , 1991

Cells

Uterine

Enzymes

Trypsin : 0.25%

Medium

HBSS

Epithelial

Mouse

Mouse, (BALB/c), male, 3-5 months

Durban, E.M.

Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix ,
In Vitro Cell Dev Biol
26 , 33 , 1990

Cells

Epithelial

Submandibular salivary gland

Enzymes

Collagenase Type 3 : 0.16% , 1:1 v/v

Medium

DMEM with 15 mM HEPES

Epithelial

Mouse

Mouse, female

Riser, M. , Huff, B. and Medina, D.

Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
19 , 730 , 1983

Cells

Epithelial

Enzymes

Pepsin : 0.1% and 0.05%

Pepsin : 0.1%

Medium

HBSS with 0.2% EDTA, CMF

Epithelial

Mouse

Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant

Yang, J. , Flynn, D. , Larson, L. and Hamamoto, S.

Growth in Primary Culture of Mouse Submandibular Epithelial Cells ,
In Vitro
18 , 435 , 1982

Cells

Epithelial

Submandibular gland

Enzymes

Collagenase Type 3 : 0.1%

Medium

HBSS

Epithelial

Mouse

Mouse (BALB/cfC3H)

Yang, J. , Guzman, R. , Richards, J. and Nandi, S.

Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels ,
In Vitro
16 , 502 , 1980

Cells

Mammary tumors

Epithelial

Enzymes

Collagenase : 1.0%

Medium

HBSS

Epithelial

Mouse

Mouse, C3H, 6-8 weeks

Lillehaug, J. , Mondal, S. and Heidelberger, C.

Establishment of Epithelial Cell Lines from Mouse Regenerating Liver ,
In Vitro
15 , 910 , 1979

Cells

Epithelial

Enzymes

Collagenase : 0.10%

Medium

DMEM

Organoids

Mouse

Fujimura, T. , Enomoto, Y. , Katsura, H. , Ogawa, T. , Baba, S. , Ogata, A. , Yamaoka, A. , Shiroguchi, K. and Morimoto, M.

Identifying a Lung Stem Cell Subpopulation by Combining Single-Cell Morphometrics, Organoid Culture, and Transcriptomics. ,
Stem Cells
41 , 809-820 , 2023

Cells

Lung

Enzymes

Collagenase Type 1 : 450 u/ml

Elastase : 1 u/ml

Neutral Protease : 5 u/ml

Deoxyribonuclease I : 0.005%

Medium

DMEM/F12

Organoids

Mouse

Klemke, L. , Blume, J. , Oliveira, T. and Schulz-Heddergott, R.

Preparation and Cultivation of Colonic and Small Intestinal Murine Organoids Including Analysis of Gene Expression and Organoid Viability. ,
Bio Protoc
12 , e4298 , 2022

Cells

Colonic tumor organoids

Enzymes

Collagenase Type 1 : 0.2%

Medium

PBS

Organoids

Mouse

Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.

Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021

Cells

Organoid cultures

Enzymes

Stemxyme 1 : 0.1%

Medium

PBS

Organoids

Mouse

Charifou, E. , Sumbal, J. , Koledova, Z. , Li, H. and Chiche, A.

A Robust Mammary Organoid System to Model Lactation and Involution-like Processes. ,
Bio Protoc
11 , e3996 , 2021

Cells

Mammary organoids

Enzymes

Collagenase Type 1 : 0.2%

Trypsin : 0.2%

Medium

DMEM/F12

Organoids

Mouse

Mouse, adult

Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al

Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
175 , 1972 , 2018

Cells

Organoids

Enzymes

Collagenase Type 4 : 200-300 u/ml

Medium

DMEM

Organoids

Mouse

Mouse, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.

Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
6 , 2017

Cells

Lung organoids

Enzymes

Neutral Protease : 24 u/ml

Deoxyribonuclease I : 0.001%

Medium

DMEM/F12

Organoids

Mouse

O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.

Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
6 , 2016

Cells

Intestinal organoids

Enzymes

Collagenase Type 4 : 500 u/ml

Deoxyribonuclease I : 15 u/ml

Medium

Basal Medium

Organoids

Mouse

Mouse, 8-12 week

Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E.

Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. ,
EMBO J
32 , 2708-21 , 2013

Cells

Pancreas organoid

Enzymes

Neutral Protease : 0.012%

Collagenase : 0.012%

Medium

DMEM

Organoids

Mouse

Xue, X. and Shah, Y.

In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
, e50210 , 2013