Tissue References Search Tool

Human

Emerman, J. and Wilkinson, D.

Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
26 , 1186 , 1990

Cells

Epithelial

Enzymes

Collagenase : 2.0%

Medium

DMEM/Ham's F-12

Epithelial

Human

Gruenert, D.C. , Basbaum, C.B. and Widdicombe, J.H.

Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions ,
In Vitro Cell Dev Biol
26 , 411 , 1990

Cells

Epithelial

Enzymes

Pronase : 0.1%

Medium

PBS

Epithelial

Human

Wood, L. and Neufeld, E.

A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux ,
J Biol Chem
265 , 12796 , 1990

Cells

Epithelial

Sweat gland

Enzymes

Collagenase Type 2 : 0.2%

Medium

(see reference)

Epithelial

Human

Human
(also bovine)

Munson, L. , Chandler, S. and Schlafer, D.

Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells ,
J Tiss Cul Meth
11 (3) , 129 , 1988

Cells

Endometrial epithelial

Enzymes

Trypsin

Medium

DMEM/Ham's F-12

Epithelial

Human

Widdicombe, J.H. , Coleman, D.L. , Finkbeiner, W.E. and Tuet, I.K.

Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa ,
J Appl Physiol
58 , 1729 , 1985

Cells

Epithelial

Enzymes

Deoxyribonuclease I : 0.01%

Medium

HEPES with 5.9mM Glucose,5mM DTT

Epithelial

Human

Yankaskas, J. , Cotton, C. , Knowles, M. , Gatzy, J. and Boucher, R.

Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports ,
Am Rev Respir Dis
132 , 1281 , 1985

Cells

Epithelial

Enzymes

Protease Type XIV : 0.1%

Medium

Eagle's MEM

Epithelial

Human

Human, women, 27-49 years

Auersperg, N. , Siemens, C.H. and Myrdal, S.E.

Human Ovarian Surface Epithelium In Primary Culture ,
In Vitro
20 , 743 , 1984

Cells

Epithelial

Ovary

Enzymes

Trypsin : 0.125%

Medium

HBSS, CMF

Epithelial

Human

Human, infant and neonate

Lechner, J. , Babcock, M. , Marnell, M. , Narayan, K. and Kaighn, M.

Normal Human Prostate Epithelial Cell Cultures ,
Methods Cell Biol
21 , 195 , 1980

Cells

Epithelial

Prostate

Enzymes

Trypsin : 0.1%

Medium

HBSS

Epithelial

Mouse

Mouse, 8-12 week

Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.

Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
6 , 2020

Cells

Nasal epithelium

Enzymes

Papain : 20 u/ml

Deoxyribonuclease I : 0.005%

Medium

EBSS

File

11787.pdf (4.95 MB)

Epithelial

Mouse

Sun, F. , Xiao, G. and Qu, Z.

Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
7 , 2017

Cells

Alveolar epithelial

Enzymes

Neutral Protease : 0.1%

Deoxyribonuclease I : 0.01%

Medium

DMEM

File

11524.pdf (669.07 KB)

Epithelial

Mouse

Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J.

Parent Stem Cells Can Serve as Niches for Their Daughter Cells. ,
Nature
523 , 597-601 , 2015

Cells

Tracheal epithelial

Enzymes

Papain : 50 u/ml

Medium

See reference

File

11556.pdf (3.75 MB)

Epithelial

Mouse

Mouse, adult

Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.

Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
Chapter 1 , Unit 1H.4 , 2011

Cells

Cilary epithelial

Enzymes

PDS kit : per instructions

Medium

EBSS

Epithelial

Mouse

Mouse, male, 8-16 week

Breggia, A. and Himmelfarb, J.

Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
1 , 33 , 2008

Cells

Renal tubular epithelial

Enzymes

Collagenase : 200 u/ml

Soybean Trypsin Inhibitor : see reference

Medium

HBSS

Epithelial

Mouse

Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.

Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
446 , 77-106 , 2008

Cells

Epithelial

Enzymes

Collagenase : 0.025%

Neutral Protease : 0.25%

Medium

DMEM

File

11599.pdf (370.99 KB)

Epithelial

Mouse

Mouse, 6-8 week

Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.

Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
168(1) , 411 , 2002

Cells

Lamina propria mononuclear cells

Enzymes

Collagenase Type 2 : 0.015%

Deoxyribonuclease I : 0.01%

Medium

RPMI

Epithelial

Mouse

Mouse, 11 week

Mueller, S. , Clark, J. , Myers, P. and Korach, K.

Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
143 , 2357 , 2002

Cells

Epithelial

Enzymes

Collagenase Type 3 : 25 u/ml

Hyaluronidase : 0.1%

Protease XIV : 0.05%

Deoxyribonuclease I : 0.04%

Medium

DMEM/F12

Epithelial

Mouse

Mouse, female

Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y.

Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis ,
Am J Pathol
155 , 173-81 , 1999

Cells

Salivary gland epithelial

Enzymes

Collagenase Type 1 : 750 u/ml

Hyaluronidase : 500 u/ml

Medium

DMEM/F12

Epithelial

Mouse

Mouse, fetal 12-13 day

Simon-Assmann, P. and Kedinger, M

Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995

Cells

Intestinal mesenchymal and epithelial

Enzymes

Collagenase : 0.03%

Medium

DMEM

Epithelial

Mouse

Durban, E.

Submandibular Salivary Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:2.1 , 1995

Cells

Submandibular salivary

Enzymes

Collagenase Type 2 or 3 : 0.16%

Medium

DMEM

Epithelial

Mouse

Katayama, M. and Kan, M.

Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation ,
In Vitro Cell Dev Biol
27 , 533 , 1991

Cells

Esophageal

Enzymes

Trypsin : 0.25%

Medium

PBS, CMF

Epithelial

Mouse

Mouse, mature, female, 6-8-wk-old

Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.

Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes ,
In Vitro Cell Dev Biol
27A , 713 , 1991

Cells

Uterine

Enzymes

Trypsin : 0.2%

Medium

HBSS

Epithelial

Mouse

Mouse, female, 18 - 20 days
(also 20 - 22 days)

Fukamachi, H. and McLachlan, J.

Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum ,
In Vitro Cell Dev Biol
27A , 907 , 1991

Cells

Uterine

Enzymes

Trypsin : 0.25%

Medium

HBSS

Epithelial

Mouse

Mouse, (BALB/c), male, 3-5 months

Durban, E.M.

Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix ,
In Vitro Cell Dev Biol
26 , 33 , 1990

Cells

Epithelial

Submandibular salivary gland

Enzymes

Collagenase Type 3 : 0.16% , 1:1 v/v

Medium

DMEM with 15 mM HEPES

Epithelial

Mouse

Mouse, female

Riser, M. , Huff, B. and Medina, D.

Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
19 , 730 , 1983

Cells

Epithelial

Enzymes

Pepsin : 0.1% and 0.05%

Pepsin : 0.1%

Medium

HBSS with 0.2% EDTA, CMF

File

532.pdf (1.61 MB)

Epithelial

Mouse

Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant

Yang, J. , Flynn, D. , Larson, L. and Hamamoto, S.

Growth in Primary Culture of Mouse Submandibular Epithelial Cells ,
In Vitro
18 , 435 , 1982

Cells

Epithelial

Submandibular gland

Enzymes

Collagenase Type 3 : 0.1%

Medium

HBSS

Epithelial

Mouse

Mouse (BALB/cfC3H)

Yang, J. , Guzman, R. , Richards, J. and Nandi, S.

Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels ,
In Vitro
16 , 502 , 1980

Cells

Mammary tumors

Epithelial

Enzymes

Collagenase : 1.0%

Medium

HBSS

Epithelial

Mouse

Mouse, C3H, 6-8 weeks

Lillehaug, J. , Mondal, S. and Heidelberger, C.

Establishment of Epithelial Cell Lines from Mouse Regenerating Liver ,
In Vitro
15 , 910 , 1979

Cells

Epithelial

Enzymes

Collagenase : 0.10%

Medium

DMEM

Epithelial

Porcine

Porcine, 5-60 kg

Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M.

Growth of Cultured Porcine Retinal Pigment Epithelial Cells ,
Acta Opthalmol Scand
81(2) , 170 , 2003

Cells

Retinal pigment epithelial cells

Enzymes

Collagenase : 2%

Medium

DMEM

Epithelial

Porcine

De Buysscher, E. , Kennedy, J. and Mendicino, J.

Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis ,
In Vitro
20 , 433 , 1984

Cells

Trachea

Enzymes

Neutral Protease : 0.2%

Medium

HBSS

File

534.pdf (3.77 MB)

Epithelial

Rabbit

Rabbit, New Zealand white, adult

Vidrich, A. , Racindranath, R. , Farsi, K. and Targan, S.

A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures ,
In Vitro Cell Dev Biol
24 (3) , 188 , 1988

Cells

Colon

Enzymes

Neutral Protease : 0.3%

Medium

PBS

Epithelial

Rabbit

Rabbit, New Zealand white, male, 4-5lb.

Chew, C.S. and Brown,M.R.

Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa ,
Biochim Biophys Acta
888 , 116 , 1986

Cells

Gastric

Parietal and chief

Enzymes

Collagenase Type 2 : 0.08%

Medium

Sodium phosphate buffer

Epithelial

Rabbit

Rabbit, New Zealand white estrous, female, 4-5 months

Nicosia, S. , Johnson, J. and Streibel, E.

Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) ,
Int J Gynecol Pathol
3 , 348 , 1984

Cells

Mesothelial and surface epithelial

Ovaries

Enzymes

Trypsin : 0.125%-0.5%

Medium

Medium 199

Epithelial

Rabbit

Rabbit, fetal

Logsdon, C.D. , Bisbee, C.A. , Rutten, M.J. and Machen, T.E.

Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel ,
In Vitro
18 , 233 , 1981

Cells

Epithelial

Gastric

Enzymes

Collagenase Type 3 : 0.10%

Medium

HBSS

Epithelial

Rat

Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.

The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. ,
Toxicol Sci
115 , 412 , 2010

Cells

Mammary epithelial

Enzymes

Collagenase Type 3 : 0.35%

Medium

HBSS

Epithelial

Rat

Rat, SD, adult, male, 8-10 weeks old

Abou-Haila, A. and Tulsiani, D.

Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
3 (1) , 35 , 2001