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Tissue References Search Tool

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Mammary
Mouse
Mouse, BALB/cfC3H/Crgl, virgin, female
DeOme, K. , Miyamoto, M. , Osborn, R. , Guzman, R. and Lum, K.
Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice ,
Cancer Res
 38 , 2103 , 1978
Cells
Nodule-transformed
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Mouse
Mouse (BALB/cCrgl), virgin, female, 4-8 month
White, M. , Hu, A. , Hamamoto, S. and Nandi
In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: fibroblast-free growth and serial passage ,
In Vitro
 14 , 271 , 1978
Cells
Epithelial
Mammary gland
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, BALB/cCrgl, female
(also guinea-pig)
Asch, B. and Medina, D.
Concanavalin A-Induced Agglutinability of Normal, Preneoplastic, and Neoplastic Mouse Mammary Cells ,
J Natl Cancer Inst
 61 (6) , 1423 , 1978
Cells
Swiss 3T3
Enzymes
Medium
PBS CMF
Mammary
Mouse
Mouse, (C3H/Crgl or BALB/cCrgl), 8-10 day pregnant
Emerman, J. , Enami, J. , Pitelka, D. and Nandi, S.
Hormonal Effects on Intracellular and Secreted Casein in Cultures of Mouse Mammary Epithelial Cells on Floating Collagen Membranes ,
Proc Natl Acad Sci U S A
 74 (10) , 4466 , 1977
Cells
Epithelial
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, lactating
Kerkof, P. and Abraham, S.
Preparation of Adipose Cell-Free Suspensions of Mammary Gland Parenchymal Cells from Lactating Mice ,
Methods Enzymol
 69 , 693 , 1976
Cells
Parenchymal
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Mammary
Mouse
Mouse, female, early pregnancy <10 days
Moore, D. and Lasfargues, E.
Method for the Continuous Cultivation of Mammary Epithelium ,
In Vitro
 7 , 21 , 1971
Cells
Epithelial
Enzymes
Collagenase : 0.1%
Medium
Eagle's MEM
Mammary
Mouse
Mouse, CBA, virgin, 9/10 wk
Prop, F. and Wiepjes, G.
Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse ,
Exp Cell Res
 61 , 451 , 1970
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
BSS, CMF
Mammary
Mouse
Mouse, lactating, 14-18 day
Pitelka, D. , Kerkof, P. , Gagne, H. , Smith, S. and Abraham, S.
Characteristics of Cells Dissociated from Mouse Mammary Glands. I. Method of Separation and Morphology of Parenchymal Cells from Lactating Glands ,
Exp Cell Res
 57 , 43 , 1969
Cells
Parenchymal
Enzymes
Collagenase : 0.33%
Medium
Kreb's buffer
Mammary
Mouse
Mouse, lactating, 14 day
Kopelovich, L. , Abraham, S. , McGrath, H. , DeOme, K. and Chaikoff, I.
Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland ,
Cancer Res
 26 , 1534 , 1966
Cells
Mammary
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, pregnant, 14-17 day
Daniel, C. and DeOme, K.
Growth of Mouse Mammary Glands In Vivo After Monolayer Culture ,
Science
 149 , 634 , 1965
Cells
Mammary
Enzymes
Collagenase : 0.05% - 0.1%
Medium
HBSS
Mammary
Mouse
Mouse, adult, 1-10 days pregnant
Lasfargues, E.
Cultivation and Behavior In Vitro of the Normal Mammary Epithelium of the Adult Mouse. II. Observations on the Secretory Activity ,
Exp Cell Res
 13 , 553 , 1957
Cells
Epithelial
Mammary
Enzymes
Collagenase : 0.02%
Medium
Simm's
Mammary
Rat
Rat
Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.
The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. ,
Toxicol Sci
 115 , 412 , 2010
Cells
Mammary epithelial
Enzymes
Medium
HBSS
Mammary
Rat
Rat, female, 50 day
Maffini M. , Soto A. , Calabro J. , Ucci A. and Sonnenschein C.
The Stroma as a Crucial Target in Rat Mammary Gland Carcinogenesis ,
J Cell Sci
 117 , 1495 , 2004
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat, female
Djuric Z. , Lewis S. , Mayhugh M. , Naegeli L. , Tang N. and Hart R.
Effect of Varying Caloric Restriction Levels on Female Rat Growth and 5-hydroxymethyl-2'-deoxyuridine in DNA ,
Toxicol Sci
 66 , 125 , 2002
Cells
Mammary gland epithelial
Enzymes
Medium
see reference
Mammary
Rat
Rat, SD
Brake P. , Zhang L. and Jefcoate C.
Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids ,
Mol Pharmacol
 54 , 825 , 1998
Cells
Mammary fibroblasts
Enzymes
Medium
DMEM/F-12
Mammary
Rat
Rat, SD, 50 day
Varela L. , Darcy K. and Ip M.
The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells ,
Endocrinology
 138 , 3891 , 1997
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat
(also human)
Soriano, J. , Pepper, M. , Nakamura, T. , Orci, L. and Montesano, R.
Hepatocyte Growth Factor Stimulates Extensive Development of Branching Duct-like Structures by Cloned Mammary Gland Epithelial Cells ,
J Cell Sci
 108 , 413-430 , 1995
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Rat
Rat, SD, female, 60-90 day
Laduca, J. and Sinha, D.
In Vitro Carcinogenesis of Mammary Epithelial Cells by N-Nitroso-N-Methylurea Using a Collagen Gel Matrix Culture ,
In Vitro Cell Dev Biol
 29A , 789 , 1993
Cells
Epithelial
Enzymes
Medium
EBSS
Mammary
Rat
Rat, Lewis, female, 90 days
Lin, T. , Hom, Y.K. , Richards, J. and Nandi, S.
Effects of Antioxidants and Reduced Oxygen Tension on Rat Mammary Epithelial Cells in Culture ,
In Vitro Cell Dev Biol
 27A , 191 , 1991
Cells
Epithelial
Mammary
Enzymes
Collagenase : 0.05%
Medium
Medium 199
Mammary
Rat
Rat, SD, female, 55 day
Ehmann, U. , Osborn, R. , Guzman, R. and Fajardo, L.
Cultured Proliferating Rat Mammary Epithelial Cells ,
In Vitro Cell Dev Biol
 27 , 749 , 1991
Cells
Epithelial
Enzymes
Neutral Protease : 3 u/ml
Medium
Medium 199
Mammary
Rat
Rat, 50-60 days
Hahm, H.A. and Ip, M.M.
Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors ,
In Vitro Cell Dev Biol
 26 , 791 , 1990
Cells
Epithelial
Enzymes
Medium
EBSS
Mammary
Rat
Rat, Fisher 344, virgin, 80-100 day
McGrath, M. , Palmer, S. and Nandi, S.
Differential Response of Normal Rat Mammary Epithelial Cells to Mammogenic Hormones and EGF ,
J Cell Physiol
 125 , 182 , 1985
Cells
Epithelial
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Rat
Rat, LEW, virgin, female, 45-50 day
Ethler, S.
Primary Culture and Serial Passage of Normal and Carcinogen-Treated Rat Mammary Epithelial Cells In Vitro ,
J Natl Cancer Inst
 74 (6) , 1307 , 1985
Cells
Epithelial
Enzymes
Collagenase : 0.1%
Medium
Medium 199
Mammary
Rat
Rat, SD, female
Cohen, L.
Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma ,
In Vitro
 18 , 565 , 1982
Cells
Epithelial, cancer and tumor
Enzymes
Collagenase : 0.1%
Medium
Eagles's MEM
Mammary
Rat
Rat, inbred LEW, female, 50-60 day old
Richards, J. and Nandi, S.
Primary Culture of Rat Mammary Epithelial Cells. I. Effect of Plating Density, Horomones, and Serum on DNA Synthesis ,
J Natl Cancer Inst
 61 (3) , 765 , 1978
Cells
Epithelial
Enzymes
Medium
Medium 199
Mammary
Rat
Rat, Wistar, 13-18 day postpartum
Katz, J. , Wals, P. and Van de Velde, R
Lipogenesis by Acini from Mammary Gland of Lactating Rats ,
J Biol Chem
 249 , 7348 , 1974
Cells
Acini
Enzymes
Collagenase : 0.05%
Medium
HBSS
Mammary
Rat
Rat, SD, 15-20 day postpartum
Martin, R. and Baldwin, R.
Effects of Insulin on Isolated Rat Mammary Cell Metabolism: Glucose Utilization and Metabolite Patterns ,
Endocrinology
 89 , 1263 , 1971
Cells
Mammary
Enzymes
Collagenase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Mammary
Rat
Rat, virgin
Moon, R. , Janns, D. and Young, S.
Preparation of Fat Cell-"Free" Rat Mammary Gland ,
J Histochem Cytochem
 17 (3) , 182 , 1969
Cells
Mammary
Enzymes
Collagenase : 0.35%
Medium
Medium 199
Miscellaneous
Bovine
Bovine
Lee, J. , Cheung, K. and Leung, V.
Systematic Study of Cell Isolation from Bovine Nucleus Pulposus: Improving Cell Yield and Experiment Reliability. ,
J Orthop Res
 33 , 1743-55 , 2015
Cells
Nucleus pulposus
Enzymes
Medium
DMEM
File
11670.pdf (6.9 MB)
Miscellaneous
Equine
Equine
Nemoto, M. , Kizaki, K. , Yamamoto, Y. , Oonuma, T. and Hashizume, K.
Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration. ,
J Equine Sci
 24 , 17-24 , 2013
Cells
Tendon Cells
Enzymes
Medium
DMEM
File
11259.pdf (104 KB)
Miscellaneous
Goat
Goat
Arora, A. , Sriram, M. , Kothari, A. and Katti, D.
Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering. ,
Cytotherapy
 19 , 881-894 , 2017
Cells
Infrapatellar fat pad mesenchymal stromal
Enzymes
Collagenase : 0.15%
Medium
DMEM
File
11573.pdf (867.29 KB)
Miscellaneous
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
File
Miscellaneous
Human
Human
Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C.
Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. ,
Nat Commun
 11 , 4061 , 2020
Cells
Adhesion fibroblasts
Enzymes
Medium
Medium 199
File
11769.pdf (9.79 MB)
Miscellaneous
Human

Human

Yoon, S. , Elahi, L. , Pașca, A. , Marton, R. , Gordon, A. , Revah, O. , Miura, Y. , Walczak, E. , Holdgate, G. , Fan, H. , Huguenard, J. , Geschwind, D. and Pașca, S.
Reliability of Human Cortical Organoid Generation. ,
Nat Methods
 16 , 75-78 , 2019
Cells
Cortical spheroids
Enzymes
Papain : 40 u/ml
Medium
EBSS
File
Miscellaneous
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
File
Miscellaneous
Human
Human
Sun, Y. , Lv, M. , Zhou, L. , Tam, V. , Lv, F. , Chan, D. , Wang, H. , Zheng, Z. , Cheung, K. and Leung, V.
Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation. ,
Osteoarthritis Cartilage
 23 , 1194-203 , 2015
Cells
Nucleus pulposus
Enzymes
Pronase : 0.25%
Collagenase Type 2 : 600 u/ml
Medium
DMEM
File
11674.pdf (3.08 MB)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.