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Tissue References Search Tool

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Muscle
Guinea-Pig
Guinea-pig, adult, 250-350 g
Jennings, L. , Xu, Q. , Firth, T. , Nelson, M. and Mawe, G.
Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle ,
Am J Physiol
 277 , G1017 , 1999
Cells
Smooth muscle
Gallbladder
Enzymes
Papain : 0.1%
Medium
NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES
Muscle
Guinea-Pig
Guinea-pig, Dunkin-Hartley, female
Ryder, K. , Bryant, S. and Hart, G.
Membrane Current Changes in Left Ventricular Myocytes Isolated From Guinea-Pigs After Abdominal Aortic Coarctation ,
Cardiovasc Res
 27 , 1278 , 1993
Cells
Myocytes
Enzymes
Protease
Medium
DMEM
Muscle
Guinea-Pig
Guinea-pig
(also rat, rabbit)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Guinea-Pig
Guinea-pig, 200-300 g
Ohya, Y. and Sperelakis, N.
ATP Regulation of the Slow Calcium Channels in Vascular Smooth Muscle Cells ,
Circ Res
 64 , 145 , 1989
Cells
Smooth muscle
Mesenteric artery
Enzymes
Collagenase : 0.3%
Medium
CF solution
Muscle
Guinea-Pig
Guinea-pig, 200-400 g
(also rabbit, frog, dogfish)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Guinea-Pig
Guinea-pig, prepubertal
Ross, R.
The Smooth Muscle Cell . II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers ,
J Cell Biol
 50 , 172 , 1971
Cells
Smooth muscle
Aortic
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt modification of Eagle's
Muscle
Hamster
Hamster, male, 60-70 day
Nakamura, T. , Iwata, Y. , Sampaolesi, M. , Hanada, H. , Saito, N. , Artman, M. , Coetzee, W. and Shigekawa, M.
Stretch-Activated Cation Channels in Skeletal Muscle Myotubes From Sarcoglycan-Deficient Hamsters ,
Am J Physiol Cell Physiol
 281 , C690 , 2001
Cells
Satellite
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
Malysz, J. , Rovner, E. , Wake, R. and Petkov, G.
Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents. ,
J Vis Exp
 , 2020
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Papain : 0.1-0.2%
Medium
Digestion solution (see reference)
File
11789.pdf (1.07 MB)
Muscle
Human
Human
Garcia, S. , Tamaki, S. , Lee, S. , Wong, A. , Jose, A. , Dreux, J. , Kouklis, G. , Sbitany, H. , Seth, R. , Knott, P. , Heaton, C. , Ryan, W. , Kim, E. , Hansen, S. , Hoffman, W. and Pomerantz, J.
High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells. ,
Stem Cell Reports
 10 , 1160-1174 , 2018
Cells
Muscle satellite
Enzymes
Collagenase : 0.1%
Trypsin : 0.25%
Medium
DMEM
File
11609.pdf (5.17 MB)
Muscle
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
File
11440.pdf (4.34 MB)
Muscle
Human
Human
Lu, S. , Sun, X. , Hong, T. , Song, K. , Yang, S. and Wang, C.
Isolation and Culture of Smooth Muscle Cells from Human Acute Type A Aortic Dissection. ,
J Cardiothorac Surg
 8 , 83 , 2013
Cells
Smooth muscle
Enzymes
Medium
DMEM
File
10920.pdf (3.09 MB)
Muscle
Human
Human, male
Stadler, G. , Chen, J. , Wagner, K. , Robin, J. , Shay, J. , Emerson, C. and Wright, W.
Establishment of Clonal Myogenic Cell Lines from Severely Affected Dystrophic Muscles - CDK4 Maintains the Myogenic Population. ,
Skelet Muscle
 1 , 12 , 2011
Cells
Myogenic
Enzymes
Medium
HBSS
Muscle
Human
Human
Nesti, L. , Jackson, W. , Shanti, R. , Koehler, S. , Aragon, A. , Bailey, J. , Sracic, M. , Freedman, B. , Giuliani, J. and Tuan, R.
Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue. ,
J Bone Joint Surg Am
 90 , 2390 , 2008
Cells
Muscle derived multiprogenitor cells
Enzymes
Medium
DMEM
Muscle
Human
Human
Moss, S. , Bates, M. , Parrino, P. and Woods, TC.
Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue. ,
Ochsner J
 7 , 133 , 2007
Cells
Endothelial and vascular smooth muscle
Enzymes
Medium
HBSS
Muscle
Human
Human
Kimuli, M. , Eardley, I. and Southgate, J.
In Vitro Assessment of Decellularized Porcine Dermis as a Matrix for Urinary Tract Reconstruction. ,
BJU Int
 94 , 859 , 2004
Cells
Urinary tract smooth muscle
Enzymes
Collagenase Type 4 : 100 u/ml
Medium
DMEM
Muscle
Human
Human, female
Richardson, M. , Taylor, D. , Casey, M. , MacDonald, P. and Stull, J.
Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture ,
In Vitro Cell Dev Biol
 23 , 21 , 1987
Cells
Smooth muscle
Myometrial
Enzymes
Deoxyribonuclease I : 0.015% and 0.007%
Medium
HBSS
Muscle
Human
Human, female
Casey, M. , MacDonald, P. , Mitchell, M. and Snyder, J.
Maintenance and Characterization of Human Myometrial Smooth Muscle Cells in Monolayer Culture ,
In Vitro
 20 , 396 , 1984
Cells
Smooth muscle
Myometrial
Enzymes
Medium
HBSS
Muscle
Human
Human, fetal
(also bovine)
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Muscle
Human
Human
Eskin, S. , Sybers, H. , Lester, J. , Navarro, L. , Gotto, A. and DeBakey, M.
Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall ,
In Vitro
 17 (8) , 713 , 1981
Cells
Smooth muscle
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
(also rat, guinea-pig, chick, monkey)
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
 59 , 1 , 1979
Cells
Smooth muscle
Enzymes
Trypsin : 0.05% - 0.1%
Medium
HBSS
Muscle
Lizard
Lizard (Anolis carolinensis)
Cox, P. and Simpson, Jr., S.
A Microphotometric STudy of Myogenic Lizard Cells Grown in Vitro ,
Dev Biol
 23 , 433 , 1970
Cells
Myoblasts, tail
Enzymes
Collagenase : 0.2%
Medium
GM III
(see reference)
Muscle
Monkey
Monkey (Macaca nemestrina)
Chait, A. , Ross, R. , Albers, J. and Bierman, E.
Platelet-Derived Growth Factor Stimulates Activity of LDL Receptors ,
Proc Natl Acad Sci U S A
 77 , 4084 , 1980
Cells
Smooth muscle
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt
Muscle
Monkey
Rhesus monkey, 1 year
(also human, rabbits)
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture ,
Cell Tissue Res
 177 , 503 , 1977
Cells
Smooth muscle, saphenous vein
Enzymes
Elastase : 0.05%
Medium
BSS
Muscle
Mouse

Mouse

Ghaibour, K. , Rizk, J. , Ebel, C. , Ye, T. , Philipps, M. , Schreiber, V. , Metzger, D. and Duteil, D.
An Efficient Protocol for CUT&RUN Analysis of FACS-Isolated Mouse Satellite Cells. ,
J Vis Exp
 , 2023
Cells
Satellite cells
Enzymes
Medium
DMEM/F-12
File
Muscle
Mouse
Huttner,S. , Ahrens, H. , Schmidt, M. , Henze, H. , Jung, M. , Schuler. S. and von Maltzahn, J.
Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle. ,
Methods Mol Biol
 , 2019
Cells
Myofiber
Enzymes
Medium
DMEM
File
11701.pdf (395.67 KB)
Muscle
Mouse
Mouse
Eckhardt, J. , Bachmann, C. , Sekulic-Jablanovic, M. , Enzmann, V. , Park, K. , Ma, J. , Takeshima, H. , Zorzato, F. and Treves, S.
Extraocular Muscle Function is Impaired in ryr3−/− Mice ,
J Gen Physiol
 151 , 929-943 , 2019
Cells
Extraocular muscles
Enzymes
Elastase : 0.0125-0.1%
Collagenase Type 2 : 0.03-0.2%
Medium
Ringer
File
11765.pdf (2.44 MB)
Muscle
Mouse

Mouse, adult

Altman, T. , Geller, D. , Kleeblatt, E. , Gradus-Perry, T. and Perlson, E.
An in vitro Compartmental System Underlines the Contribution of Mitochondrial Immobility to the ATP Supply in the NMJ. ,
J Cell Sci
 132 , 2019
Cells
Skeletal muscle
Enzymes
Medium
See reference
File
Altman.2019.pdf (10.3 MB)
Muscle
Mouse
Mouse, 6-8 week
Sachdeva, J. , Mahajan, A. , Cheng, J. , Baeten, J. , Lilly, B. , Kuivaniemi, H. and Hans, C.
Smooth Muscle Cell-Specific Notch1 Haploinsufficiency Restricts the Progression of Abdominal Aortic Aneurysm by Modulating CTGF Expression. ,
PLoS ONE
 12 , e0178538 , 2017
Cells
vascular smooth muscle
Enzymes
Elastase : 0.75 u/ml
Medium
DMEM/F12
File
11761.pdf (4.01 MB)
Muscle
Mouse
Mouse, GFP transgenic
Birbrair, A, Sattiraju, A, Zhu, D, Zulato, G, Batista, I, Nguyen, V, Messi M, Solingapuram Sai, K, Marini, F, Delbono, O. and Mintz, A.
Novel Peripherally Derived Neural-Like Stem Cells as Therapeutic Carriers for Treating Glioblastomas. ,
Stem Cells Transl Med
 , 2016
Cells
Muscle derived NLSC
Enzymes
Medium
DMEM
File
11498.pdf (2.18 MB)
Muscle
Mouse
Mouse, 2-4 month
Randolph, M. , Phillips, B. , Choo, H. , Vest, K. , Vera, Y. and Pavlath, G.
Pharyngeal Satellite Cells Undergo Myogenesis Under Basal Conditions and Are Required for Pharyngeal Muscle Maintenance. ,
Stem Cells
 33 , 3581-95 , 2015
Cells
Pharyngeal myofiber
Enzymes
Collagenase Type 1 : 400-800 u/ml
Medium
DMEM
File
11584.pdf (2.55 MB)
Muscle
Mouse
Mouse, 2 month
Abou-Khalil, R. , Yang, F. , Lieu, S. , Julien, A. , Perry, J. , Pereira, C. , Relaix, F. , Miclau, T. , Marcucio, R. and Colnot, C.
Role of Muscle Stem Cells During Skeletal Regeneration. ,
Stem Cells
 33 , 1501-11 , 2015
Cells
Myoblasts
Enzymes
Collagenase Type 2 : 400 u/ml
Neutral Protease : 0.5 u/ml
Medium
Hams F-10
Muscle
Mouse
Mouse, 6-8 week
Pasut, A. , Jones, A. and Rudnicki, M.
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle. ,
J Vis Exp
 73 , e50074 , 2013
Cells
Myofibers
Enzymes
Medium
DMEM
File
10979.pdf (336.09 KB)
Muscle
Mouse
Mouse, 10 week
Rezk, B. , Yoshida, T. , Semprun-Prieto, L. , Higashi, Y. , Sukhanov, S. and Delafontaine, P.
Angiotensin II Infusion Induces Marked Diaphragmatic Skeletal Muscle Atrophy. ,
PLoS ONE
 7 , e30276 , 2012
Cells
Diaphragm cells
Enzymes
Collagenase Type 2 : 100 u/ml
Pronase : 0.125%
Medium
PBS
File
11078.pdf (2.1 MB)
Muscle
Mouse
Mouse
Weisleder, N. , Zhou, J. and Ma, J.
Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers. ,
Methods Mol Biol
 798 , 395 , 2012
Cells
Skeletal muscle fiber
Enzymes
Medium
Tyrode
File
11084.pdf (1.59 MB)
Muscle
Mouse
Mouse
Metz, R. , Patterson, J. and Wilson, E.
Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization. ,
Methods Mol Biol
 843 , 169 , 2012
Cells
Vascular smooth muscle
Enzymes
Medium
Basal Culture
Muscle
Mouse
Mouse
Shi, H. , Boadu, E. , Mercan, F. , Le, A. , Roth Flach, R. , Zhang, L. , Tyner, K. , Olwin, B. and Bennett, A.
MAP Kinase Phosphatase-1 Deficiency Impairs Skeletal Muscle Regeneration and Exacerbates Muscular Dystrophy. ,
FASEB J
 24 , 2985 , 2010
Cells
Myoblast
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.