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Tissue References Search Tool

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Neural
Rat
Rat, 1-4 day old
Twitchell, W. , Brown, S. and Mackie, K.
Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons ,
J Neurophysiol
 78 , 43 , 1997
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
MEM
Neural
Rat
Rat, E18
Liu QY, Schaffner AE, Li YX, Dunlap V, Barker JL.
Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons ,
J Neurosci
 16 , 2912-23 , 1996
Cells
Hippocampal neurons
Enzymes
Papain : 20 u/ml
Medium
MEM
Neural
Rat
Rat, 1-6 day
Robertson, S. , Rae, M. , Rowan, E. and Kennedy, C.
Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia. ,
Br J Pharmacol
 118 , 951 , 1996
Cells
DRG neurons
Enzymes
Papain : 20 u/ml
Collagenase Type 1 : 150 u/ml
Medium
HBSS
File
10618.pdf (2.97 MB)
Neural
Rat
Rat, pups, 14 day old
McFarlane, S. and Cooper, E.
Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons ,
J Neurosci
 13 (6) , 2591 , 1993
Cells
Neurons, sympathetic
Enzymes
Medium
HBSS
Neural
Rat
Rats, SD, 11-14 day old, 29-32 g
Allen, T. , Sim, J. and Brown, D.
The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat ,
J Physiol
 460 , 91 , 1993
Cells
Basal forebrain neurons
Enzymes
Trypsin : 0.125%
Medium
Gey's BSS
Neural
Rat
Rat, Wistar-Hanover, 7-12 day
Rayport, S. , Sulzer, D. , Shi, W. , Sawasdikosol, S. , Monaco, J. , Batson, D. and Rajendran, G.
Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology ,
J Neurosci
 12 (11) , 4264 , 1992
Cells
Postnatal dopamine neurons
Enzymes
Trypsin : 0.035%
Medium
(see reference)
Neural
Rat
Rat
Stemple, D. and Anderson, D.
Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest ,
Cell
 71 , 973 , 1992
Cells
Stem, neural crest
Enzymes
Collagenase : 0.075%
Medium
Ringer's solution
Neural
Rat
Rat, SD, 18-day-old, fetus
Cheng, B. and Mattson, M.
IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage ,
J Neurosci
 12 (4) , 1558 , 1992
Cells
Hippocampal
Enzymes
Trypsin : 0.2%
Medium
HBSS
Neural
Rat
Rat, SD, pregnant, Charles River
Buchhalter, J. and Dichter, M.
Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus ,
Brain Res Bull
 26 , 333 , 1991
Cells
Pyramidal neurons
Nonpyramidal neurons
Enzymes
Trypsin : 0.027%
Medium
HEPES
Neural
Rat
Rat, SD, embryos, 19-21 days gestation
Mithen, F. , Reiker, M. and Birchem, R.
Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture ,
In Vitro Cell Dev Biol
 26 , 129 , 1990
Cells
Schwann, dorsal root ganglia
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Rat
Rat
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Neural
Rat
Rat, SD, 8 day old pups
Novelli, A. , Reilly, J. , Lysko, P. and Henneberry, R.
Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced ,
Brain Res
 451 , 205 , 1988
Cells
Cerebellar neurons
Enzymes
Trypsin : 0.025%
Medium
Eagle's MEM
Neural
Rat
Rat, fetuses, 18 day old
Mattson, M. and Kater, S.
Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda ,
Int J Dev Neurosci
 6 (5) , 439 , 1988
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Medium
Eagle's MEM
Neural
Rat
Rat, postnatal
Hatanaka,H. , Tsukui, H. and Nihonmatsu, I.
Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor ,
Neurosci Lett
 79 , 85 , 1987
Cells
Septal neurons
Enzymes
Papain : 0.05%
Medium
PBS, CMF
Neural
Rat
Rat, SD, female, 4 month old
Wood, P. and Bunge, R.
Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals ,
Nature
 320 , 756 , 1986
Cells
CNS cells
Enzymes
Trypsin : 0.25%
Medium
EBSS
Neural
Rat
Rat, Long Evans, 1-15 days
Huettner, J. and Baughman, R.
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
 6 , 3044 , 1986
Cells
Neurons, visual cortex
Enzymes
Papain : 20 u/ml
Medium
BSS
(see reference)
Neural
Rat
Rat, embryo, 15 day
Jahr, C. and Jessell, T.
Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate ,
J Neurosci
 5 , 2281 , 1985
Cells
Dorsal horn neurons
Spinal
Enzymes
Trypsin : 0.025%
Medium
Ham's F-12
Neural
Rat
Rat, postnatal
Leifer, D. , Lipton, S. , Barnstable, C. and Masland, R.
Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture ,
Science
 224 , 303 , 1984
Cells
Ganglion, retina
Enzymes
Papain : 12.5 u/ml
Medium
HBSS w/5 mM HEPES
Neural
Rat
Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
 4 , 1944 , 1984
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Neural
Rat
Rat, W/FU, 5-8 day
Raff, M. , Fields, K. , Hakomori, S. , Mirsky, R. , Pruss, R. and Winter, J.
Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture ,
Brain Res
 174 , 283 , 1979
Cells
Neurons and glial
Enzymes
Trypsin : 0.25%
Medium
MEM
(see reference)
Neural
Rat
Rat, fetus
Wakshull, E. , Johnson, M. and Burton, H.
Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons ,
J Neurophysiol
 42 , 1410 , 1979
Cells
Neurons, sympathetic
Enzymes
Trypsin : 0.25%
Medium
L-15 or HBSS, CMF
Neural
Rat
Rat, Wistar/Furth, newborn
(also bovine)
Brockes, J. , Fields, K. and Raff, M.
Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve ,
Brain Res
 165 , 105 , 1979
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryo
Dichter, M.
Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation ,
Brain Res
 149 , 279 , 1978
Cells
Neurons, cortical
Enzymes
Trypsin : 0.027%
Medium
MEM
Neural
Rat
Rat, newborn
Reichardt, L. and Patterson, P.
Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures ,
Nature
 270 , 147 , 1977
Cells
Neurons, sympathetic
Enzymes
Medium
Hank's solution, CF
Neural
Rat
Rat, neonatal
Mains, R. and Patterson, P.
Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties ,
J Cell Biol
 59 , 329 , 1973
Cells
Neurons, superior cervical ganglia
Enzymes
Trypsin : 0.1%
Medium
Basal L-15 medium
Neural
Salamander
Salamander, 18-25 cm
Townes-Anderson, E. , MacLeish, P. and Raviola, E.
Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase ,
J Cell Biol
 100 , 175 , 1985
Cells
Retina
Enzymes
Papain : 14 u/ml
Medium
Saline
Neural
Salamander
Salamander (A.tigrinum)
Bader, C. , MacLeish, P. and Schwartz, E.
Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina ,
Proc Natl Acad Sci U S A
 75 , 3507 , 1978
Cells
Photoreceptors, retina
Enzymes
Papain : 0.05%
Medium
(see reference)
Neural
Shellfish
Aplysia californica
Lee, A. , Decourt, B. and Suter, D.
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones. ,
J Vis Exp
 12 , 662 , 2008
Cells
Neurons
Enzymes
Medium
L-15-ASW
File
10491.pdf (280.15 KB)
Neural
Shellfish
Snails (Helisoma trivolis), albino, adult
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Neural
Shellfish
Pond snail (Helisoma) albino, adult
Haydon, P.
The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata ,
J Neurosci
 8 , 1032 , 1988
Cells
Somata, buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Antibiotic saline, Leibowitz 50%
Neural
Shellfish
Helisoma trivovlis
Cohan, C. , Haydon, P. and Kater, S.
Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma ,
J Neurosci Res
 13 , 285 , 1985
Cells
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
L-15 medium
Neural
Shellfish
Mollusc, juvenile 1-2 gm or adult 50-100 gm
Schacher, S. and Proshanksy, E.
Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment ,
J Neurosci
 3 (12) , 2403 , 1983
Cells
Neurons
LUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Neural
Shellfish
Aplysia californica
Camardo, J. , Proshansky, E. and Schacher, S.
Identified Aplysia Neurons Form Specific Chemical Synapses in Culture ,
J Neurosci
 3 (12) , 2614 , 1983
Cells
LUQ cells
RUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Neural
Turtle
Turtle (Pseudemys scripta elegans)
Lam, D.
Biosynthesis of Acetylcholine in Turtle Photoreceptors ,
Proc Natl Acad Sci U S A
 69 , 1987 , 1972
Cells
Retinal
Enzymes
Papain : 0.1% (13.5 u/mg)
Medium
Kreb's Ringer
Organoids
Human

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.
Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
 15 , 7027 , 2024
Cells
Brain organoids
Enzymes
Papain : 20 u/ml
Medium
EBSS
File

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.