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Tissue References Search Tool

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Neural
Mouse
Mouse, 6-8 week
Malin, S. , Davis, B. and Molliver, D.
Production of Dissociated Sensory Neuron Cultures and Considerations for their use in Studying Neuronal Function and Plasticity ,
Nat Protoc
 2 , 152 , 2007
Cells
Sensory neurons, DRG
Enzymes
Papain : 20 u/ml
Medium
HBSS
Neural
Mouse
Mouse
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Mouse
Mouse, adult
Gonzalez John M, Bergmann Cornelia C, Fuss Babette, Hinton David R, Kangas Cindy, Macklin Wendy B, Stohlman Stephen A.
Expression of a dominant negative IFN-gammareceptor on mouse oligodendrocytes ,
Glia
 51 , 22-34 , 2005
Cells
Brain and spinal cord cells
Enzymes
Trypsin : 0.25%
Medium
PBS
Neural
Mouse
Mouse, C57BL/6, 1 year
Eide, L. and McMurray, C
Culture of Adult Mouse Neurons ,
Biotechniques
 38(1) , 99-104 , 2005
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
DMEM
Neural
Mouse
Mouse, neonatal
Gill JC, Moenter SM, Tsai PS.
Developmental regulation of gonadotropin-releasing hormone neurons by fibroblast growth factor signaling ,
Endocrinology
 145 , 3830 , 2004
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
EBSS
Neural
Mouse
Mouse, C57BL
Okano-Uchida T, Himi T, Komiya Y. and Ishizaki Y
Cerebellar granule cell precursors can differentiate into astroglial cells ,
Proc Natl Acad Sci U S A
 101 , 1211 , 2004
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 16.5 u/ml
Medium
Dulbecco's PBS
Neural
Mouse
Mouse, 1-3 day
Savchenko V, Sung U, Blakely RD.
Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody ,
Mol Cell Neurosci
 24 , 1131 , 2003
Cells
Neurons, ganglia
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 100 u/ml
Collagenase : 0.3%
Trypsin : 0.05%
Medium
HBSS
Neural
Mouse
Mouse, male
Roberts, L. and MacDonald, C.and Mark, C.
Anandamide is a Partial Agonist at Native Vanilloid Receptors in Acutely Isolated Mouse Trigeminal Sensory Neurons. ,
Br J Pharmacol
 137 , 421 , 2002
Cells
Trigeminal senesory neurons
Enzymes
Papain : 20 u/ml
Medium
HEPES buffered saline
Neural
Mouse
Mouse, postnatal-day-1-old (P1)
Deshmukh, M. , Kuida, K. and Johnson Jr., E.
Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization ,
J Cell Biol
 150 (1) , 131 , 2000
Cells
Neurons
Enzymes
Trypsin : 0.25%
Medium
NGF-containing medium
Neural
Mouse
Mouse embryos
Lee, K. , Davies, A. and Jaenisch, R.
P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF ,
Development
 120 , 1027 , 1994
Cells
Neurons, DRG and SCG
Enzymes
Trypsin : 0.25%
Medium
L-15 medium
Neural
Mouse
mouse, 65 days
Richards L. , Kilpatrick T. and Bartlett P.
De Novo Generation of Neuronal Cells from the Adult Mouse Brain ,
Proc Natl Acad Sci U S A
 89 , 8591 , 1992
Cells
Neurons, neuronal precursors
Enzymes
Trypsin : 0.1%
Medium
DMEM
Neural
Mouse
Mouse, CD-1, neonate
Quinn, S. and De Boni, U.
Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro ,
In Vitro Cell Dev Biol
 27 , 55 , 1991
Cells
Neurons, dorsal root ganglion
Enzymes
Trypsin : 0.25%
Medium
HBSS
Neural
Mouse
Mouse, fetal
Kitani, H. , Shiurba, R. , Sakakura, T. and Tomooka, Y.
Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture ,
In Vitro Cell Dev Biol
 27 , 615 , 1991
Cells
Precursor
Enzymes
Trypsin : 0.5%
Medium
PBS
Neural
Mouse
Mouse (SWR or CF1), 1-3 months
Spielman, A. , Mody, I. , Brand, J. , Whitney, G. , MacDonald, J. and Salter, M.
A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells ,
Brain Res
 503 , 326 , 1989
Cells
Papillae, taste receptor
Enzymes
Pronase E : 0.15%
Medium
Carbonate-Phosphate buffer
(see reference)
Neural
Mouse
Mouse, neonatal
(also chick)
Varon, S. , Skaper, S. , Barbin, G. , Selak, I. and Manthorpe, M.
Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System ,
J Neurosci
 4 (3) , 654 , 1984
Cells
PNS test neurons
Enzymes
Trypsin : 0.08%
Medium
Eagle's Basal Medium (see reference)
Neural
Mouse
Mouse (BALB/c), adult
Eagleson, K. and Bennett, M.
Survival of Purified Motor Neurons In Vitro: Effects of Skeletal Muscle-Conditioned Medium ,
Neurosci Lett
 38 , 187 , 1983
Cells
Neurons, spinal cord
Enzymes
Medium
Hank's BSS, CMF
Neural
Mouse
Mouse, 0-30 day
Shrier, B. , Wilson, S. and Nirenberg, M.
Cultured Cell Systems and Methods for Neurobiology , Vol. 32 , 765 , 1974
Cells
Neural
Enzymes
Medium
BSS
Neural
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Neural
Porcine
Porcine, adult, 60-100 kg
Si ML, Lee TJ.
Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries ,
J Pharmacol Exp Ther
 298 , 122 , 2001
Cells
Superior cervical ganglia
Enzymes
Papain : 2 u/ml
Collagenase : 0.12%
Medium
HBSS
Neural
Quail
Quail
Sieber-Blum, M. and Cohen, A.
Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells ,
Dev Biol
 80 , 96 , 1980
Cells
Neural crest
Enzymes
Trypsin : 0.05%
Medium
MEM, HBSS
Neural
Rat
Rat, 2-3 week
Lin, Y. and Chen, J.
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release. ,
J Vis Exp
 , 2018
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
File
11814.pdf (1.07 MB)
Neural
Rat
Rat, SD, 3 month
Andersen, N. and Monje, P.
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers. ,
Methods Mol Biol
 1739 , 49-66 , 2018
Cells
Schwann
Enzymes
Medium
DMEM
File
11612.pdf (854.59 KB)
Neural
Rat
Rat, SD, day 2
Maurel, P.
Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies. ,
Methods Mol Biol
 1739 , 17-37 , 2018
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
File
11615.pdf (837.77 KB)
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
File
11611.pdf (3.73 MB)
Neural
Rat
Rat, Wistar, 3 day
Fangmann, L. , Teller, S. , Stupakov, P. , Friess, H. , Ceyhan, G. and Demir, I.
3D Cancer Migration Assay with Schwann Cells. ,
Methods Mol Biol
 1739 , 317-325 , 2018
Cells
Dorsal root ganglia neurons
Enzymes
Medium
MEM
File
11614.pdf (311.34 KB)
Neural
Rat
Rat, adult
George, D. , Ahrens, P. and Lambert, S.
Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells. ,
Glia
 , 2018
Cells
Schwann
Enzymes
Stemxyme 1 : 0.05%
Medium
DMEM
File
11631.pdf (1.14 MB)
Neural
Rat
Rat, SD, p7
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
File
11677.pdf (7.17 MB)
Neural
Rat
Rat, E17
Hayakawa, K. , Esposito, E. , Wang, X. , Terasaki, Y. , Liu, Y. , Xing, C. , Ji. X. and Lo, E.
Transfer of Mitochondria from Astrocytes to Neurons after Stroke. ,
Nature
 535 , 551-5 , 2016
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
File
11680.pdf (3.94 MB)
Neural
Rat
Rat, SD
Andersen, N. , Srinivas, S. , Pinero, G. and Monje, P.
A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves. ,
Sci Rep
 6 , 31781 , 2016
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
File
11697.pdf (3.48 MB)
Neural
Rat
Rat, SD
Schuh, C. , Hercher, D. , Stainer, M. , Hopf, R. , Teuschl, A. , Schmidhammer, R. and Redl, H.
Extracorporeal Shockwave Treatment: A Novel Tool to Improve Schwann Cell Isolation and Culture. ,
Cytotherapy
 18 , 760-70 , 2016
Cells
Schwann
Enzymes
Medium
DMEM
File
11564.pdf (1.2 MB)
Neural
Rat
Rat, 6-8 week
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
File
11551.pdf (632.51 KB)
Neural
Rat
Rat, embryonic
Oyanagi, K. , Tashiro, T. and Negishi, T.
Cell-Type-Specific and Differentiation-Status-Dependent Variations in Cytotoxicity of Tributyltin in Cultured Rat Cerebral Neurons and Astrocytes. ,
J Toxicol Sci
 40 , 459-68 , 2015
Cells
Cerebral neurons
Enzymes
Papain : 2 u/ml
Medium
DMEM-PBS
File
11424.pdf (1.5 MB)
Neural
Rat
Rat, SD, 6-9 week
Murayama, C. , Watanabe, S. and Nakamura, M. and, Norimoto, H.
Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons. ,
Molecules
 20 , 14959-69 , 2015
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
File
11445.pdf (2.96 MB)
Neural
Rat
Rat, embryonic and neonatal
Rheaume, C. , Cai, B. , Wang, J. , Fernandez-Salas, E. , Aoki, K. , Francis, J. and Broide, R.
A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25. ,
Toxins
 7 , 2354-70 , 2015
Cells
Cortical neurons, DRG
Enzymes
PDS kit : per instructions
Medium
Neurobasal
File
11421.pdf (2.49 MB)
Neural
Rat
Rat
Yu, R. , Seymour, V. , Berecki, G. , Jia, X. , Akca, M. , Adams, D. , Kaas, Q. and Craik, D.
Less is More: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic α-Conotoxin Vc1.1. ,
Sci Rep
 5 , 13264 , 2015
Cells
Dorsal root ganglion neurons
Enzymes
Medium
HBSS
File
11450.pdf (1.23 MB)
Neural
Rat
Rat, SD
Wei, X. , Yan, J. , Tillu, D. , Asiedu, M. , Weinstein, N. , Melemedjian, O. , Price, T. and Dussor, G.
Meningeal Norepinephrine Produces Headache Behaviors in Rats via Actions Both on Dural Afferents and Fibroblasts. ,
Cephalalgia
 35 , 1054-64 , 2015
Cells
Trigeminal ganglion
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
File
11606.pdf (691.01 KB)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.