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Tissue References Search Tool

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Skin
Human
Human, ages 16-30
Pedersen, P.
Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis ,
In Vitro Cell Dev Biol
 25 (4) , 342 , 1989
Cells
Human sweat duct
Enzymes
Medium
RPMI 1640
(see reference)
Skin
Human
Human
Peacocke, M. , Yaar, M. , Mansur, C. , Chao, M. and Gilchrest, B.
Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes ,
Proc Natl Acad Sci U S A
 85 , 5282 , 1988
Cells
Melanocytes, skin/ foreskin
Enzymes
Trypsin : 0.25%
Medium
PBS
Skin
Human
Human
Lee, C. , Carpenter, F. , Coaker, T. and Kealey, T.
The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands ,
J Cell Sci
 83 , 103 , 1986
Cells
Human sweat glands
Enzymes
Collagenase : 0.2%
Medium
HBSS
Skin
Human
Human
Dover, R. and Potten, C.
Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes ,
J Invest Dermatol
 80 , 423 , 1983
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, 18-30 years, male, female
McCoy, B. , Galdun, J. and Cohen, I.
Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts ,
In Vitro
 18 (1) , 79 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.1%
Medium
HBSS
Skin
Human
Human, adult
Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A.
Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells ,
J Cell Biol
 94 , 497-505 , 1982
Cells
Epidermal keratinocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.2%
Medium
Eagle's MEM
Skin
Human
Human, fetal
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Skin
Human
Human, newborn
Liu, S. , Eaton, M. and Karasek, M.
Growth Characteristics of Human Epidermal Keratinocytes from Newborn Foreskin in Primary and Serial Cultures ,
In Vitro
 15 (10) , 813 , 1979
Cells
Keratinocytes
Enzymes
Trypsin : 0.3%
Medium
DMEM
Skin
Human
Human
Rheinwald, J. and Green, H.
Serial Cultivation of Strains of Human Epidermal Kerotinocytes: The Formation of Keratinizing Colonies from Single Cells ,
Cell
 6 , 331 , 1975
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
(see reference)
Skin
Mouse
Mouse
Spidale, N. , Malhotra, N. , Frascoli, M. , Sylvia, K. , Miu, B. , Freeman, C. , Stadinski, B. , Huseby, E. and Kang, J.
Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent Spontaneous Atopic Dermatitis ,
Elife
 9 , 5118 , 2020
Cells
Keratinocytes
Enzymes
Medium
DMEM
File
11729.pdf (6.68 MB)
Skin
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media
File
11675.pdf (9.69 MB)
Skin
Mouse
Mouse
Rinkevich, Y. , Walmsley, G. , Hu, M. , Maan, Z. , Newman, A. , Drukker, M. , Januszyk, M. , Krampitz, G. , Gurtner, G. , Lorenz, H. , Weissman, I. and Longaker, M.
Identification and Isolation of a Dermal Lineage with Intrinsic Fibrogenic Potential. ,
Science
 348 , aaa2151 , 2015
Cells
Dermal fibroblasts
Enzymes
Elastase : 0.012%
Medium
DMEM
File
11625.pdf (4.88 MB)
Skin
Mouse
Mouse
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
File
11796.pdf (2.39 MB)
Skin
Mouse
Mouse
Jensen, U. , Ghazizadeh, S. and Owens, D.
Isolation and Characterization of Cutaneous Epithelial Stem Cells. ,
Methods Mol Biol
 989 , 61-9 , 2013
Cells
Epithelial stem
Enzymes
Trypsin : 0.25%
Medium
HBSS
File
11456.pdf (386.28 KB)
Skin
Mouse
Mouse
Gu, D. , Fan, Q. and Xie, J.
Cell Population Analyses During Skin Carcinogenesis. ,
J Vis Exp
 , e50311 , 2013
Cells
Epidermal
Enzymes
Medium
DMEM
File
11061.pdf (216.27 KB)
Skin
Mouse
Mouse
Antsiferova, M. , Martin, C. , Huber, M. , Feyerabend, T. , Forster, A. , Hartmann, K. , Rodewald, H. , Hohl, D. and Werner, S.
Mast Cells are Dispensable for Normal and Activin-Promoted Wound Healing and Skin Carcinogenesis. ,
J Immunol
 191 , 6147-55 , 2013
Cells
Mast
Enzymes
Medium
IMDM
File
11627.pdf (1.93 MB)
Skin
Mouse
Mouse
Scheibye-Knudsen, M. , Ramamoorthy, M. , Sykora, P. , Maynard, S. , Lin, P. , Minor, R. , Wilson, D. , Cooper, M. , Spencer, R. , de Cabo, R. , Croteau, D. and Bohr, V.
Cockayne Syndrome Group B Protein Prevents the Accumulation of Damaged Mitochondria by Promoting Mitochondrial Autophagy. ,
J Exp Med
 209 , 855 , 2012
Cells
Dermal fibroblasts
Enzymes
Collagenase : 400 u/ml
Medium
AminoMAX II
File
10934.pdf (6.3 MB)
Skin
Mouse
Mouse, 2-3 day
Farina, G. , York, M. , Di Marzio, M. , Collins, C. , Meller, S. , Homey, B. , Rifkin, I. , Marshak-Rothstein, A. , Radstake, T. and Lafyatis, R.
Poly(I:C) Drives Type I IFN- and TGFβ-Mediated Inflammation and Dermal Fibrosis Simulating Altered Gene Expression in Systemic Sclerosis. ,
J Invest Dermatol
 130 , 2583-93 , 2010
Cells
Dermal fibroblasts
Enzymes
Trypsin : 0.025%
Medium
DME
File
11013.pdf (1.72 MB)
Skin
Mouse
Mouse
King, I. , Kroenke, M. and Segal, B.
GM-CSF-Dependent, CD103+ Dermal Dendritic Cells Play a Critical Role in Th Effector Cell Differentiation After Subcutaneous Immunization. ,
J Exp Med
 207 , 953 , 2010
Cells
Epidermal and dermal
Enzymes
Medium
PBS
File
10588.pdf (4.98 MB)
Skin
Mouse
Mouse
Eidsmo, L. , Allan, R. , Caminschi, I. , Van Rooijen, N. , Heath, W. and Carbone, F.
Differential Migration of Epidermal and Dermal Dendritic Cells During Skin Infection. ,
J Immunol
 182 , 3165 , 2009
Cells
Dermal
Enzymes
Medium
HBSS
File
10587.pdf (935.27 KB)
Skin
Mouse
Mouse, neonatal
Crigler Lauren, Kazhanie Amita, Yoon Tae-Jin, Zakhari Julia, Anders Joanna, Taylor Barbara, Virador VictoriaM.
Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages ,
FASEB J
 21 , 2050-63 , 2007
Cells
Dermal
Enzymes
Collagenase : 0.35%
Deoxyribonuclease I : see reference
Medium
PBS
Skin
Mouse
mouse
Baxter Ruth M, Crowell Thomas P, McCrann Margaret E, Frew Erica M, Gardner Humphrey.
Analysis of the tight skin (Tsk1/+) mouse as a model for testing antifibrotic agents ,
Lab Invest
 85 , 1199-209 , 2005
Cells
Dermal fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Skin
Mouse
Mouse, male 6-24 week
Montanaro F, Liadaki K, Volinski J, Flint A. and Kunkel LM.
Skeletal muscle engraftment potential of adult mouse skin side population cells ,
Proc Natl Acad Sci U S A
 100 , 9336 , 2003
Cells
Skin side population
Enzymes
Medium
PBS
Skin
Mouse
Mouse
Takanami-Ohnishi Yoko, Amano Shinya, Kimura Sadao, Asada Sachie, Utani Atsushi, Maruyama Masumi, Osada Hiroyuki, Tsunoda Hajime, Irukayama-Tomobe Yoko, Goto Katsutoshi, Karin Michael, Sudo Tatsuhiko, Kasuya Yoshitoshi.
Essential role of p38 mitogen-activated protein kinase in contact hypersensitivity ,
J Biol Chem
 277 , 37896-903 , 2002
Cells
Ear epidermal
Enzymes
Trypsin : 0.1%
Collagenase : 0.2%
Medium
PBS
Skin
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Skin
Porcine
Porcine, 3-4 month
Ando, W. , Kutcher, J. , Krawetz, R. , Sen, A. , Nakamura, N. , Frank, C. and Hart, D.
Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage. ,
Cytotherapy
 16 , 776 , 2014
Cells
Synovial membrane and skin stem
Enzymes
Collagenase : 0.2%
Medium
DMEM
Skin
Porcine
Porcine, 2-6 month
Regauer S, Compton C.
Cultured porcine epithelial grafts: an improved method ,
J Invest Dermatol
 94 , 230-4 , 1990
Cells
Keratinocytes
Enzymes
Medium
Dulbecco-Vogt MEM
Skin
Rabbit

Rabbit, Leporidae

Santos, F. , Carvalho, C. , Almeida, I. , Fagulha, T. , Rammos, F. , Barros, S. , Henriques, M. , Luís, T. and Duarte, M.
Simple Method for Establishing Primary Leporidae Skin Fibroblast Cultures. ,
Cells
 10 , 2021
Cells
Fibroblast
Enzymes
Trypsin : 0.25%
Medium
DMEM/HAMSF12
File
Santos2021.pdf (1.93 MB)
Skin
Rat
Rat, Wistar, 4-8 day
Sugihara, H. and Toda, S
Primary Tissue Intact and Dissociated Cell Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 3A:2.1 , 1995
Cells
Dermal fibroblasts and keratinocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Skin
Rat
Rat, SD, male, 40-60 days
Laurent, S. , Mednieks, M. and Rosenfield, R.
Growth of Sebaceous Cells in Monolayer Culture ,
In Vitro Cell Dev Biol
 28 , 83 , 1992
Cells
Sebaceous
Enzymes
Trypsin : 0.2%
Medium
DMEM with FBS
PBS
Skin
Rat
Rat, neonatal, 12-24 hr post partum
Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.
Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
 7 , 265 , 1991
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
HEPES buffered DMEM
Skin
Rat
Rat, albino, one day old, CFN
Vaughan, F. , Gray, R. and Bernstein, I.
Growth and Differentiation of Primary Rat Keratinocytes on Synthetic Membranes ,
In Vitro Cell Dev Biol
 22 (3) , 141 , 1986
Cells
Keratinocytes
Enzymes
Trypsin : 1%
Medium
EBSS
Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
File
Comeau.2023.pdf (4.03 MB)
Spleen
Mouse
Mouse, 4 week
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
 11 , 55 , 2020
Cells
Trophoblast
Enzymes
Medium
DMEM
File
11723.pdf (5.09 MB)
Spleen
Mouse
Mouse
Villarreal, E. , Hewgley, W. , Lang, W. , Morton, C. , Mao, S. , Wu, J. and Sandoval, J.
In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation? ,
J Surg Res
 218 , 209-216 , 2017
Cells
Speen
Enzymes
Medium
HBSS
File
11826.pdf (758.25 KB)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.