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Tissue References Search Tool

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Stem
Human
Human
Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V. and De Kock, J
Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods. ,
Cytotherapy
 16 , 1220-8 , 2014
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
File
11264.pdf (3.44 MB)
Stem
Human
Human
Douvaras, P. , Wang, J. , Zimmer, M. , Hanchuk, S. , O'Bara, M. , Sadiq, S. , Sim, F. , Goldman, J. and Fossati, V.
Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. ,
Stem Cell Reports
 3 , 250 , 2014
Cells
Skin fibroblasts
Enzymes
Collagenase Type 1 : 1000 u/ml
Medium
DMEM
File
11453.pdf (5.29 MB)
Stem
Human
Human
Hang, H. , Yu, Y. , Wu, N. , Huang, Q. , Xia, Q. and Bian, J.
Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction. ,
PLoS ONE
 9 , e104133 , 2014
Cells
Umbilical mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM
File
11073.pdf (3.59 MB)
Stem
Human
Human, adult
Koellensperger, E. , Bollinger, N. , Dexheimer, V. , Gramley, F. , Germann, G. and Leimer, U.
Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities. ,
Cytotherapy
 16 , 789 , 2014
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Stem
Human
Human, adult
Al-Saqi, S. , Saliem, M. , Asikainen, S. , Quezada, H. , Ekblad, A. , Hovatta, O. , Le Blanc, K. , Jonasson, A. and Gotherstrom, C.
Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 16 , 915 , 2014
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
see reference
Stem
Human
Human
Avolio, E. , Gianfranceschi, G. , Cesselli, D. , Caragnano, A. , Athanasakis, E. , Katare, R. , Meloni, M. , Palma, A. , Barchiesi, A. , Vascotto, C. , Toffoletto, B. , Mazzega, E. , Finato, N. , Aresu, G. and Livi, U.
Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction. ,
Stem Cells
 32 , 2373 , 2014
Cells
Cardiac stem
Enzymes
Medium
Joklik modified Eagle’s
File
11468.pdf (6.86 MB)
Stem
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
File
11040.pdf (5.93 MB)
Stem
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
File
11538.pdf (301.63 KB)
Stem
Human
Human
Han, Y. , Tao, R. , Sun, T. , Chai, J. , Xu, G. and Liu, J.
Optimization of Human Umbilical Cord Mesenchymal Stem Cell Isolation and Culture Methods. ,
Cytotechnology
 65 , 819 , 2013
Cells
Umbilical cord mesenchymal stem
Enzymes
Collagenase : 0.1-0.2%
Trypsin : 0.1%
Medium
DMEM
File
11452.pdf (506.14 KB)
Stem
Human
Human, adult
Nagel, S. , Rohr, F. , Weber, C. , Kier, J. , Siemers, F. , Kruse, C. , Danner, S. , Brandenburger, M. and Matthiessen, A.
Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro. ,
PLoS ONE
 8 , e78365 , 2013
Cells
Epidermal stem cells
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 2.5%
Medium
DMEM
File
11024.pdf (2.66 MB)
Stem
Human
Human
Salmon, B. , Bardet, C, Khaddam, M. Naji, J. , Coyac, B. , Baroukh, B. , Letourneur, F. , Lesieur, J. , Decup, F. , Le Denmat, D. , Nicoletti, A. , Poliard, A, Rowe, P. , Huet, E. , Vital, S. and Linglart, A.
MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia. ,
PLoS ONE
 8 , e56749 , 2013
Cells
Dental pulp stem cells
Enzymes
Medium
DMEM
Stem
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Stem
Human
Human
Doi, K. , Tanaka, S. , Iida, H. , Eto, H. , Kato, H. , Aoi, N. , Kuno, S. , Hirohi, T. and Yoshimura, K.
Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis. ,
J Tissue Eng Regen Med
 7 , 864 , 2013
Cells
Stromal vascular fraction
Enzymes
Medium
PBS
File
11052.pdf (437.6 KB)
Stem
Human
Human
Bonnamain, V. , Thinard, R. , Sergent-Tanguy, S. , Huet, P. , Bienvenu, G. , Naveilhan, P. , Farges, J. and Alliot-Licht, B.
Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium. ,
Front Physiol
 4 , 357 , 2013
Cells
Dental pulp stem
Enzymes
Medium
DMEM/F12
File
11025.pdf (1.24 MB)
Stem
Human
Human
Carvalho, P. , Gimble, J. , Dias, I. , Gomes, M. and Reis, R.
Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells. ,
Tiss Eng
 19 , 473-8 , 2013
Cells
Adipose stromal stem
Enzymes
Medium
DMEM/Hams F-12
File
10891.pdf (866.48 KB)
Stem
Human
Human
Welte, Y. , Davies, C. , Schafer, R. and Regenbrecht, C.
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma. ,
J Vis Exp
 73 , e50200 , 2013
Cells
Melanoma
Enzymes
Medium
PBS
File
11032.pdf (822.27 KB)
Stem
Human
Human
Sakai, K. , Yamamoto, A. , Matsubara, K. , Nakamura, S. , Naruse, M. , Yamagata, M. , Sakamoto, K. , Tauchi, R. , Wakao, N. , Imagama, S. , Hibi, H. , Kadomatsu, K. , Ishiguro, N. and Ueda, M
Human Dental Pulp-Derived Stem Cells Promote Locomotor Recovery After Complete Transection of the Rat Spinal Cord by Multiple Neuro-Regenerative Mechanisms. ,
J Clin Invest
 122 , 80 , 2012
Cells
Dental pulp derived stem
Enzymes
Medium
DMEM
Stem
Human
Human
Cervelli, V. , Scioli, M. , Gentile, P. , Doldo, E. , Bonanno, E. , Spagnoli, L. and Orlandi, A.
Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance. ,
Stem Cells Transl Med
 1 , 206-20 , 2012
Cells
Adipose-derived stem
Enzymes
Medium
PBS
Stem
Human
Human
Gentile, P. , Orlandi, A. , Scioli, M. , Di Pasquali, C. , Bocchini, I. and Cervelli, V.
Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery. ,
Stem Cells Transl Med
 1 , 230-6 , 2012
Cells
Adipose derived stromal vascular
Enzymes
Medium
PBS
Stem
Human
Human
Salehinejad, P. , Alitheen, N. , Ali, A. , Omar, A. , Mohit, M. , Janzamin, E. , Samani, F. , Torshizi, Z. and Nematollahi-Mahani, S.
Comparison of Different Methods for the Isolation of Mesenchymal Stem Cells from Human Umbilical Cord Wharton's Jelly. ,
In Vitro Cell Dev Biol Anim
 48 , 75 , 2012
Cells
Wharton jelly mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM
Stem
Human
Human
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta ,
Regenerative Research
 1 , 48 , 2012
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F12
File
10916.pdf (791.32 KB)
Stem
Human
Human
Nazarov, I. , Lee, J. , Soupene, E. , Etemad, S. , Knapik, D. , Green, W. , Bashkirova, E. , Fang, X. , Matthay, M. , Kuypers, F. and Serikov, V.
Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. ,
Stem Cells Transl Med
 1 , 359-72 , 2012
Cells
Chorionic mesenchymal
Enzymes
Neutral Protease : 2.5 u/ml
Trypsin : 0.25%
Medium
MEM
Stem
Human
Human
James, A. , Zara, J. , Corselli, M. , Askarinam, A. , Zhou, A. , Hourfar, A. , Nguyen, A. , Megerdichian, S. , Asatrian, G. , Pang, S. , Stoker, D. , Zhang, X. , Wu, B. , Ting, K. , Peault, B. and Soo, C.
An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering. ,
Stem Cells Transl Med
 1 , 673 , 2012
Cells
Perivascular stem
Enzymes
Medium
DMEM
Stem
Human
Human
Vellasamy, S. , Sandrasaigaran, P. , Vidyadaran, S. , George, E. and Ramasamy, R.
Isolation and Characterisation of Mesenchymal Stem cells Derived from Human Placenta Tissue. ,
World J Stem Cells
 4 , 53-61 , 2012
Cells
Placental mesenchymal stem
Enzymes
Medium
DMEM
File
11457.pdf (291.83 KB)
Stem
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
File
11031.pdf (467.49 KB)
Stem
Human
Human, male 40-60 years
Blasi, A. , Martino, C. , Balducci, L. , Saldarelli, M. , Soleti, A. , Navone, S. , Canzi, L. , Cristini, S. , Invernici, G. , Parati, E. and Alessandri, G.
Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential ,
Vasc Cell
 3 , 5 , 2011
Cells
Adipose derived stem cells
Enzymes
Collagenase : 0.25%
Medium
PBS
Stem
Human
Human
Yang, X. , He, X. , He, J. , Zhang, L. , Su, X. , Dong, Z. , Xu, Y. , Li, Y. and Li, YL.
High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells. ,
J Biomed Sci
 18 , 59 , 2011
Cells
Adipose derived mesenchymal
Enzymes
Medium
L-DMEM
File
10930.pdf (7.71 MB)
Stem
Human
Human
Klein, D. , Weisshardt, P. , Kleff, V. , Jastrow, H. , Jakob, H. and Ergun, S.
Vascular Wall-Resident CD44+ Multipotent Stem Cells Vive Rise to Pericytes and Smooth Muscle Cells and Contribute to New Vessel Maturation. ,
PLoS ONE
 6 , e20540 , 2011
Cells
Vascular wall-resident multipotent stem cells
Enzymes
Elastase : 5 u/ml
Medium
see reference
File
10879.pdf (1009.69 KB)
Stem
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
File
11598.pdf (768.38 KB)
Stem
Human
Human
Farias, V. , Linares-Fernandez, J. , Penalver, J. , Paya Colmenero, J. , Ferron, G. , Duran, E. , Fernandez, R. , Olivares, E. , O'Valle, F. , Puertas, A. , Oliver, F. and Ruiz de Almodovar, J.
Human Umbilical Cord Stromal Stem Cell Express CD10 and Exert Contractile Properties. ,
Placenta
 32 , 86 , 2011
Cells
Umbilical cord stromal stem
Enzymes
Medium
DMEM
Stem
Human
Human
Yu, G. Floyd, ZE. , Wu, X. , Halvorsen, Y. and Gimble, J.
Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates. ,
Methods Mol Biol
 702 , 17-27 , 2011
Cells
Adipose derived stem
Enzymes
Medium
DMEM/Ham's F-12
Stem
Human
Human
Roig, A. , Eskiocak, U. , Hight, S. , Kim, S. , Delgado, O. , Souza, R. , Spechler, S. , Wright, W. and Shay, J.
Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro. ,
Gastroenterol
 138 , 1012 , 2010
Cells
Colonic epithelial
Enzymes
Collagenase : 150 u/ml
Neutral Protease : 0.04 mg/ml
Medium
Basal X media
Stem
Human
Human
Zeddou, M. , Briquet, A. , Relic, B. , Josse, C. , Malaise, M. , Gothot, A. , Lechanteur, C. and Beguin, Y.
The Umbilical Cord Matrix is a Better Source of Mesenchymal Stem Cells (MSC) Than the Umbilical Cord Blood. ,
Cell Biol Int
 34 , 693-701 , 2010
Cells
Umbilical cord mesenchymal
Enzymes
Hyaluronidase : 0.05%
Collagenase : 0.08%
Medium
DMEM
File
10929.pdf (709.43 KB)
Stem
Human
Human
Du, Y. , Roh, D. , Funderburgh, M. , Mann, M. , Marra, K. , Rubin, J. , Li, X. and Funderburgh, J.
Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. ,
Mol Vis
 16 , 2680 , 2010
Cells
Corneal stromal stem
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase : 0.1%
Medium
DMEM
File
10602.pdf (1.08 MB)
Stem
Human
Human
Hjelmeland, A. , Wu, Q. , Wickman, S. , Eyler, C. , Heddleston, J. , Shi, Q. , Lathia, J. , Macswords, J. , Lee, J. , McLendon, R. and Rich, J.
Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth. ,
PLoS Biol
 8 , e1000319 , 2010
Cells
Glioma stem cells
Enzymes
PDS kit : per instructions
Medium
neurobasal medium
Stem
Human
Human
Huang, G. , Yamaza, T. , Shea, L. , Djouad, F. , Kuhn, N. , Tuan, R. and Shi, S.
Stem/Progenitor Cell-Mediated De Novo Regeneration of Dental Pulp with Newly Deposited Continuous Layer of Dentin in an In Vivo Model. ,
Tissue Eng Part A
 16 , 605 , 2010
Cells
Dental pulp and apical papilla stem cells
Enzymes
Medium
MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.