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Tissue References Search Tool

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Spleen
Mouse
Mouse
Siragam, V. , Crow, A. , Brinc, D. , Song, S. , Freedman, J. and Lazarus, A.
Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells. ,
Nat Med
 12 , 688 , 2006
Cells
Leukocytes
Enzymes
Collagenase Type 4 : 43 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L
ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
 196 , 1415 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse, 4-6 week
McLellan, A. , Kapp, M. , Eggert, A. , Linden, C. , Bommhardt, U. , Brocker, E. , Kammerer, U. and Kampgen, E.
Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression ,
Blood
 99 , 2084-93 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI-1640
Spleen
Mouse
Mouse
Stagg, AJ, Burke, F, Hill, S. and Knight, S.
Isolation of Mouse Spleen Dendritic Cells. ,
Methods Mol Med
 64 , 9-22 , 2001
Cells
Dentritic cells
Enzymes
Medium
HEPES/RPMI 1640
File
11527.pdf (127.78 KB)
Spleen
Mouse
Mouse
Brasel K, De Smedt T, Smith JL, Maliszewski CR.
Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures ,
Blood
 96 , 3029-39 , 2000
Cells
Dentritic
Enzymes
Collagenase : 100 u/ml
Medium
HBSS
Stem
Canine
23-33 kg
Williams, S. , Kosnik, P. , Kleinert, L. , Vossman, E. , Lye, K. and Shine, M.
Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts. ,
Tissue Eng Part A
 19 , 1295-302 , 2013
Cells
Stromal vascular
Enzymes
Collagenase : see reference
Medium
Media 199E
File
11705.pdf (717.79 KB)
Stem
Canine
Canine
Park, S. , Seo, M. , Kim, H. and Kang, K.
Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells. ,
PLoS ONE
 7 , e44693 , 2012
Cells
Amniotic membrane MSC
Enzymes
Trypsin : 0.25%
Medium
LG-DMEM
File
10917.pdf (558.9 KB)
Stem
Canine
Canine, 20-25 kg
Fischer, L. , McIlhenny, S. , Tulenko, T. , Golesorkhi, N. , Zhang, P. , Larson, R. , Lombardi, J. , Shapiro, I. and DiMuzio, P.
Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force. ,
J Surg Res
 152 , 157 , 2009
Cells
Adipose stem cells
Enzymes
Collagenase : see reference
Medium
Media-199
File
10599.pdf (2.34 MB)
Stem
Equine
Equine
Vidal, M. , Robinson, S. , Lopez, M. , Paulsen, D. , Borkhsenious, O. , Johnson, J. , Moore, R. and Gimble, J.
Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow. ,
Vet Surg
 37 , 713 , 2008
Cells
Adipose derived stem cells
Enzymes
Medium
PBS
Stem
Equine
Equine, 1-5 year
Vidal, M. , Kilroy, G. , Lopez, M. , Johnson, J. , Moore, R. and Gimble, J.
Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells. ,
Vet Surg
 36 , 613 , 2007
Cells
Adipose derived stem cells
Enzymes
Medium
PBS
Stem
Human
Human
Alstrup, T. , Eijken, M. , Bohn, A. , Moller, B. and Damsgaard, T.
Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. ,
Curr Protoc Stem Cell Biol
 48 , e68 , 2019
Cells
Adipose derived stem
Enzymes
Collagenase Type 4 : 150-300 u/ml
Medium
RPMI-1640
File
11767.pdf (5.17 MB)
Stem
Human
Human
Yam, G. , Teo, E. , Setiawan, M. , Lovatt, M. , Yusoff, N. , Fuest, M. , Goh, B. and Mehta, J.
Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy. ,
J Cell Mol Med
 , 2018
Cells
Postnatal periodontal ligament
Enzymes
Medium
DMEM/F12
File
11613.pdf (1.35 MB)
Stem
Human
Human
Matsumura, E. , Tsuji, K. , Komori, K. , Koga, H. , Sekiya, I. and Muneta, T.
Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 19 , 181-193 , 2017
Cells
Synovial mesenchymal stem
Enzymes
Collagenase : 0.3%
Medium
MEM
File
11566.pdf (1.64 MB)
Stem
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS
File
11721.pdf (1.67 MB)
Stem
Human
Human
de Witte, S. , Lambert, E. , Merino, A. , Strini, T. , Douben, H. , O'Flynn, L. , Elliman, S. , de Klein, A. , Newsome, P. , Baan, C. and Hoogduijn, M.
Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion. ,
Cytotherapy
 19 , 798-807 , 2017
Cells
Umbilical cord mesenchymal stromal
Enzymes
Medium
MEM
File
11570.pdf (570.31 KB)
Stem
Human
Human
Oppliger, B. , Joerger-Messerli, M. , Simillion, C. , Mueller, M. , Surbek, D. and Schoeberlein, A.
Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact. ,
Cytotherapy
 19 , 829-838 , 2017
Cells
Wharton jelly mesenchymal stromal
Enzymes
Collagenase Type 2 : 270 u/ml
Medium
DMEM/F12
File
11568.pdf (1.09 MB)
Stem
Human
Human
Rigamonti, A. , Repetti, G. , Sun, C. , Price, F. , Reny, D. , Rapino, F. , Weisinger, K. , Benkler, C. , Peterson, Q. , Davidow, L. , Hansson, E. and Rubin, L.
Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System. ,
Stem Cell Reports
 6 , 993-1008 , 2016
Cells
Motor neurons
Enzymes
Papain : 20 u/ml
Medium
EBSS
File
11514.pdf (331.19 KB)
Stem
Human
Human
Randelli, P. , Menon, A. , Ragone, V. , Creo P. , Alfieri, M. , Perucca, O. , Banfi, G. , Cabitza, P. , Tettamanti, G. and Anastasia, L.
Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study. ,
BMC Complement Altern Med
 16 , 293 , 2016
Cells
Tendon stem
Enzymes
Medium
MEM
File
11512.pdf (2.2 MB)
Stem
Human
Human
Cheng, N. , Hsieh, T. , Lai, H. and Young, T.
High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells ,
Cytotherapy
 18 , 371-83 , 2016
Cells
Adipose stem
Enzymes
Medium
DMEM/F12
File
11571.pdf (782.51 KB)
Stem
Human
Human
Lim, J. , Razi, Z. , Law, J. , Nawi, A. , Idrus, R. and Ng, M.
MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord. ,
Cytotherapy
 18 , 1493-1502 , 2016
Cells
Wharton jelly mesenchymal stromal
Enzymes
Medium
DMEM
File
11565.pdf (1.9 MB)
Stem
Human
Human
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
File
11551.pdf (632.51 KB)
Stem
Human
Human
Wu, Z. , Wang, J. , Dong, R. , Wang, L. , Fan, Z. , Liu, D. and Wang, S.
Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells. ,
Int J Clin Exp Med
 8 , 7220-30 , 2015
Cells
Dental mesenchymal stem
Enzymes
Medium
DMEM
File
11426.pdf (941.35 KB)
Stem
Human
Human
Kusuma, G. , Menicanin, D. , Gronthos, S. , Manuelpillai, U. , Abumaree, M. , Pertile, M. , Brennecke, S. and Kalionis, B.
Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua. ,
PLoS ONE
 10 , e0141246 , 2015
Cells
Decidua mesenchymal stem
Enzymes
Medium
PBS
File
11455.pdf (1.2 MB)
Stem
Human
Human
Satish, L. , Krill-Burger, J. , Gallo, P. , Etages, S. , Liu, F. , Philips, B. , Ravuri, S. , Marra, K. , LaFramboise, W. , Kathju, S. and Rubin, J.
Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage. ,
BMC Med Genomics
 8 , 41 , 2015
Cells
Adipose derived stem cells
Enzymes
Medium
HBSS
File
11422.pdf (1.74 MB)
Stem
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
File
11440.pdf (4.34 MB)
Stem
Human
Human
Lee, S. , Park, B. , Kim, J. , Jekarl, D. , Choi, H. , Lee, S. , Kim, M. , Kim, Y. and Park, M.
The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells. ,
Cytotherapy
 17 , 1723-31 , 2015
Cells
Wharton jelly mesenchymal stem
Enzymes
Medium
PBS
File
11562.pdf (1.54 MB)
Stem
Human
Human
Choudhery,M, Badowski, M, Muise, A. and Harris, D
Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 359-68 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
File
11265.pdf (4.11 MB)
Stem
Human
Human
Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V. and De Kock, J
Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods. ,
Cytotherapy
 16 , 1220-8 , 2014
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
File
11264.pdf (3.44 MB)
Stem
Human
Human
Douvaras, P. , Wang, J. , Zimmer, M. , Hanchuk, S. , O'Bara, M. , Sadiq, S. , Sim, F. , Goldman, J. and Fossati, V.
Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. ,
Stem Cell Reports
 3 , 250 , 2014
Cells
Skin fibroblasts
Enzymes
Collagenase Type 1 : 1000 u/ml
Medium
DMEM
File
11453.pdf (5.29 MB)
Stem
Human
Human
Hang, H. , Yu, Y. , Wu, N. , Huang, Q. , Xia, Q. and Bian, J.
Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction. ,
PLoS ONE
 9 , e104133 , 2014
Cells
Umbilical mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM
File
11073.pdf (3.59 MB)
Stem
Human
Human, adult
Koellensperger, E. , Bollinger, N. , Dexheimer, V. , Gramley, F. , Germann, G. and Leimer, U.
Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities. ,
Cytotherapy
 16 , 789 , 2014
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Stem
Human
Human, adult
Al-Saqi, S. , Saliem, M. , Asikainen, S. , Quezada, H. , Ekblad, A. , Hovatta, O. , Le Blanc, K. , Jonasson, A. and Gotherstrom, C.
Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 16 , 915 , 2014
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
see reference
Stem
Human
Human
Avolio, E. , Gianfranceschi, G. , Cesselli, D. , Caragnano, A. , Athanasakis, E. , Katare, R. , Meloni, M. , Palma, A. , Barchiesi, A. , Vascotto, C. , Toffoletto, B. , Mazzega, E. , Finato, N. , Aresu, G. and Livi, U.
Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction. ,
Stem Cells
 32 , 2373 , 2014
Cells
Cardiac stem
Enzymes
Medium
Joklik modified Eagle’s
File
11468.pdf (6.86 MB)
Stem
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
File
11040.pdf (5.93 MB)
Stem
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
File
11538.pdf (301.63 KB)
Stem
Human
Human
Han, Y. , Tao, R. , Sun, T. , Chai, J. , Xu, G. and Liu, J.
Optimization of Human Umbilical Cord Mesenchymal Stem Cell Isolation and Culture Methods. ,
Cytotechnology
 65 , 819 , 2013
Cells
Umbilical cord mesenchymal stem
Enzymes
Collagenase : 0.1-0.2%
Trypsin : 0.1%
Medium
DMEM
File
11452.pdf (506.14 KB)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.