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Tumor
Mouse
Mouse, 30 week
Berger, M. , Bergers, G. , Arnold, B. , Hammerling, G. and Ganss, R.
Regulator of G-protein Signaling-5 Induction in Pericytes Coincides With Active Vessel Remodeling During Neovascularization ,
Blood
 105 , 1094 , 2005
Cells
Tumor endothelial
Enzymes
Medium
PBS
Tumor
Mouse
Mouse
Oliver, T. , Read, T. , Kessler, J. , Mehmeti, A. , Wells, J. , Huynh, T. , Lin, S. and Wechsler-Reya, R.
Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma. ,
Development
 132 , 2425 , 2005
Cells
Granule cell precursors, pre-neoplastic and tumor cells
Enzymes
Papain : 10 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Neurobasal/B27
Tumor
Mouse
Mouse, 8-10 week
Hida Kyoko, Hida Yasuhiro, Amin Dhara N, Flint Alan F, Panigrahy Dipak, Morton Cynthia C, Klagsbrun Michael.
Tumor-associated endothelial cells with cytogenetic abnormalities ,
Cancer Res
 64 , 8249-55 , 2004
Cells
Tumor associated endothelial cells
Enzymes
Medium
see reference
Tumor
Mouse
Mouse
Bergers Gabriele, Song Steven, Meyer-Morse Nicole, Bergsland Emily, Hanahan Douglas.
Benefits of targeting both pericytes and endothelial cells in the tumor vasculature with kinase inhibitors ,
J Clin Invest
 111 , 1287-95 , 2003
Cells
Pancreatic tumor
Enzymes
Medium
PBS
Tumor
Mouse
Mouse
Uekusa Yasuhiro, Yu Wen-Gong, Mukai Takao, Gao Ping, Yamaguchi Nobuya, Murai Masako, Matsushima Kouji, Obika Satoshi, Imanishi Takeshi, Higashibata Yuji, Nomura Shintaro, Kitamura Yukihiko, Fujiwara Hiromi, Hamaoka Toshiyuki.
A pivotal role for CC chemokine receptor 5 in T-cell migration to tumor sites induced by interleukin 12 treatment in tumor-bearing mice ,
Cancer Res
 62 , 3751-8 , 2002
Cells
Tumor-infiltrating lymphocyte
Enzymes
Collagenase : 200 u/ml
Medium
RPMI 1640
Tumor
Mouse
Mouse, male
Arbiser JL, Raab G, Rohan RM, Paul S, Hirschi K, Flynn E, Price ER, Fisher DE, Cohen C, Klagsbrun M.
Isolation of mouse stromal cells associated with a human tumor using differential diphtheria toxin sensitivity ,
Am J Pathol
 155 , 723-9 , 1999
Cells
Melanoma tumor cells
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse (BALB/cfC3H)
Yang, J. , Guzman, R. , Richards, J. and Nandi, S.
Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels ,
In Vitro
 16 , 502 , 1980
Cells
Mammary tumors
Epithelial
Enzymes
Collagenase : 1.0%
Medium
HBSS
Tumor
Mouse
Mouse, BALB/cfC3H/Crgl
Hosick, H.
A Note on Growth Patterns of Epithelial Tumor Cells in Primary Culture ,
Cancer Res
 34 , 259 , 1974
Cells
Neoplastic
Epithelial tumor
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse, lactating, 14 day
Kopelovich, L. , Abraham, S. , McGrath, H. , DeOme, K. and Chaikoff, I.
Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland ,
Cancer Res
 26 , 1534 , 1966
Cells
Mammary
Enzymes
Medium
HBSS
Tumor
Rat
Rat, adult
Duarte, S. , Momier, D. , Baque, P. , Casanova, V. , Loubat, A. , Samson, M. , Guigonis, J. , Staccini, P. , Saint-Paul, M. , De Lima, M. , Carle, G. and Pierrefite-Carle, V.
Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model. ,
Stem Cells
 31 , 423-32 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Tumor
Rat
Rat
Mehta, N. , Lopez, P. , Vyas, A. and Schnaar, R.
Gangliosides and Nogo Receptors Independently Mediate Myelin-Associated Glycoprotein Inhibition of Neurite Outgrowth in Different Nerve Cells. ,
J Biol Chem
 282 , 27875-86 , 2007
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
Hibernate A
File
11605.pdf (1.18 MB)
Tumor
Rat
Rat, male 12 week
Sharma N, Luo J, Kirschmann DA, O'Malley Y, Robbins ME, Akporiaye ET, Lubaroff DM, Heidger PM, Hendrix MJ.
A novel immunological model for the study of prostate cancer ,
Cancer Res
 59 , 2271-6 , 1999
Cells
Sponge infiltrating cells
Enzymes
Medium
RPMI 1640
Tumor
Rat
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months, rat, (ACI/MNs) male, 8 weeks
Masuda, A. , Ohtsuka, K. and Matsuyama, M.
Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
 26 , 713 , 1990
Cells
Epthelial
Enzymes
Medium
Eagle's MEM Serum-free
Tumor
Rat
Rat
Brennan, M. , Oldberg, A. , Hayman, E. and Ruoslahti, E.
Effect of a Proteoglycan Produced by Rat Tumor Cells on Their Adhesion to Fibronectin-Collagen Substrata ,
Cancer Res
 43 , 4302 , 1983
Cells
Yolk sac tumor
Enzymes
Trypsin : 0.01%
Medium
DMEM
Tumor
Rat
Rat, SD, female
Cohen, L.
Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma ,
In Vitro
 18 , 565 , 1982
Cells
Epithelial, cancer and tumor
Enzymes
Collagenase : 0.1%
Medium
Eagles's MEM
Tumor
Rat
Rat
Gazdar, A. , Chick, W. , Oie, H. , Sims, H. , King, D. , Weir, G. and Lauris, V.
Continuous, Clonal, Insulin-and Somatostatin-Secreting Cell Lines Established from a Transplantable Rat Islet Cell Tumor ,
Proc Natl Acad Sci U S A
 77 (6) , 3519 , 1980
Cells
Tumor, islet
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Tumor
Rat
Rat
Essner, E.
Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands ,
Exp Cell Res
 7 , 430 , 1954
Cells
Ascites hepatoma
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer (see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.