Worthington Tissue Dissociation Guide
Dissociating Enzymes: Chymotrypsin
Chymotrypsin is a protease which preferentially catalyzes the hydrolysis of peptide bonds involving the aromatic amino acids tyrosine, phenylalanine, and tryptophan. In addition it acts upon the peptide bonds of leucyl, methionyl, asparagenyl and glutamyl residues, and the amides and esters of susceptible amino acids.
Chymotrypsin is used to a limited extent in tissue dissociation, usually in combination with trypsin and elastase.
The Worthington Tissue Tables provide references useful to researchers interested in tissue dissociation and cell harvesting procedures. The references are organized by Tissue and Species type and linked to PubMed citations. The Cell type, Enzymes, and Medium for each reference is provided.
To search by specific criteria, use the Tissue References Search Tool.